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carl ks400 camera  (Carl Zeiss)


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    Carl Zeiss carl ks400 camera
    tPA administration accelerates revascularization in a HL ischemia model. (A) tPA or vehicle was injected intraperitoneally daily from day 0 to day 2 into tPA+/+ and tPA−/− mice after HL ischemia induction. The frequency of circulating CD45+CD11b+ cells was measured (n = 3 per group). The single dagger (†) symbol denotes a significant difference (P < .05) between day 0 and day 2 in HL tPA+/+ mice. (B) C57BL/6 mice were treated with tPA or vehicle. Macroscopic pictures of the lower limb region were taken on day 15 after HL induction. Images were acquired with 0.5× and 2.5×/0.1 numeric aperture (NA) lenses (corresponding to 5× and 25× magnification), an Olympus SZX7 camera, and Adobe Photoshop CS3. (C) The ischemic/normal blood flow ratio, determined by analysis of the whole cross-section of the limb by laser Doppler perfusion imaging analysis, is shown as a function of time. (D-G) Evaluation of necrotic areas (D-E, after 15 days; bar = 200 μm) and capillary density in sections (F-G, after 28 days; bar = 50 μm) of HL-induced mice treated with or without tPA (n = 6 per group). Panels D and F were acquired with a 20×/0.50 NA and a Carl Zeiss <t>KS400</t> camera, and analyzed using Axio Vision (Carl Zeiss). (H-I) Quantification of VEGF-A+ cells (arrows) in ischemic tissues (n = 6 per group, after 15 days; bar = 20 μm). Images were acquired with a 40×/0.75 NA and a Carl Zeiss KS400 camera, and analyzed using Axio Vision (Carl Zeiss). (J) Immunohistochemical staining of VEGFR-1 and VEGF-A of ischemic muscle tissues derived from vehicle- or tPA-treated HL ischemic mice and nonischemic mice. Arrows indicate VEGFR-1+VEGF-A+ cells (bar = 20 μm). Images were acquired with a 40×/0.75 NA and a Carl Zeiss KS400 camera, and analyzed using Axio Vision (Carl Zeiss). (K-L) tPA or vehicle was injected intraperitoneally daily into tPA (K) or Plg (L) wild-type and deficient mice after induction of HL ischemia. Functional perfusion measurements of the collateral region were performed (n = 3 per group). A significant difference between tPA+/+ and tPA−/− mice treated with BSA-PBS is shown. If not otherwise mentioned, asterisks denote significant differences between BSA-PBS– and tPA-treated groups. *P < .05; **P < .001; values represent the mean ± SEM.
    Carl Ks400 Camera, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c2+eclipse+ti+microscope/ks400+software/pmc02879105-313-10-8
    Average 90 stars, based on 1 article reviews
    carl ks400 camera - by Bioz Stars, 2026-10
    90/100 stars

    Images

    1) Product Images from "Tissue type plasminogen activator regulates myeloid-cell dependent neoangiogenesis during tissue regeneration"

    Article Title: Tissue type plasminogen activator regulates myeloid-cell dependent neoangiogenesis during tissue regeneration

    Journal: Blood

    doi: 10.1182/blood-2009-08-236851

    tPA administration accelerates revascularization in a HL ischemia model. (A) tPA or vehicle was injected intraperitoneally daily from day 0 to day 2 into tPA+/+ and tPA−/− mice after HL ischemia induction. The frequency of circulating CD45+CD11b+ cells was measured (n = 3 per group). The single dagger (†) symbol denotes a significant difference (P < .05) between day 0 and day 2 in HL tPA+/+ mice. (B) C57BL/6 mice were treated with tPA or vehicle. Macroscopic pictures of the lower limb region were taken on day 15 after HL induction. Images were acquired with 0.5× and 2.5×/0.1 numeric aperture (NA) lenses (corresponding to 5× and 25× magnification), an Olympus SZX7 camera, and Adobe Photoshop CS3. (C) The ischemic/normal blood flow ratio, determined by analysis of the whole cross-section of the limb by laser Doppler perfusion imaging analysis, is shown as a function of time. (D-G) Evaluation of necrotic areas (D-E, after 15 days; bar = 200 μm) and capillary density in sections (F-G, after 28 days; bar = 50 μm) of HL-induced mice treated with or without tPA (n = 6 per group). Panels D and F were acquired with a 20×/0.50 NA and a Carl Zeiss KS400 camera, and analyzed using Axio Vision (Carl Zeiss). (H-I) Quantification of VEGF-A+ cells (arrows) in ischemic tissues (n = 6 per group, after 15 days; bar = 20 μm). Images were acquired with a 40×/0.75 NA and a Carl Zeiss KS400 camera, and analyzed using Axio Vision (Carl Zeiss). (J) Immunohistochemical staining of VEGFR-1 and VEGF-A of ischemic muscle tissues derived from vehicle- or tPA-treated HL ischemic mice and nonischemic mice. Arrows indicate VEGFR-1+VEGF-A+ cells (bar = 20 μm). Images were acquired with a 40×/0.75 NA and a Carl Zeiss KS400 camera, and analyzed using Axio Vision (Carl Zeiss). (K-L) tPA or vehicle was injected intraperitoneally daily into tPA (K) or Plg (L) wild-type and deficient mice after induction of HL ischemia. Functional perfusion measurements of the collateral region were performed (n = 3 per group). A significant difference between tPA+/+ and tPA−/− mice treated with BSA-PBS is shown. If not otherwise mentioned, asterisks denote significant differences between BSA-PBS– and tPA-treated groups. *P < .05; **P < .001; values represent the mean ± SEM.
    Figure Legend Snippet: tPA administration accelerates revascularization in a HL ischemia model. (A) tPA or vehicle was injected intraperitoneally daily from day 0 to day 2 into tPA+/+ and tPA−/− mice after HL ischemia induction. The frequency of circulating CD45+CD11b+ cells was measured (n = 3 per group). The single dagger (†) symbol denotes a significant difference (P < .05) between day 0 and day 2 in HL tPA+/+ mice. (B) C57BL/6 mice were treated with tPA or vehicle. Macroscopic pictures of the lower limb region were taken on day 15 after HL induction. Images were acquired with 0.5× and 2.5×/0.1 numeric aperture (NA) lenses (corresponding to 5× and 25× magnification), an Olympus SZX7 camera, and Adobe Photoshop CS3. (C) The ischemic/normal blood flow ratio, determined by analysis of the whole cross-section of the limb by laser Doppler perfusion imaging analysis, is shown as a function of time. (D-G) Evaluation of necrotic areas (D-E, after 15 days; bar = 200 μm) and capillary density in sections (F-G, after 28 days; bar = 50 μm) of HL-induced mice treated with or without tPA (n = 6 per group). Panels D and F were acquired with a 20×/0.50 NA and a Carl Zeiss KS400 camera, and analyzed using Axio Vision (Carl Zeiss). (H-I) Quantification of VEGF-A+ cells (arrows) in ischemic tissues (n = 6 per group, after 15 days; bar = 20 μm). Images were acquired with a 40×/0.75 NA and a Carl Zeiss KS400 camera, and analyzed using Axio Vision (Carl Zeiss). (J) Immunohistochemical staining of VEGFR-1 and VEGF-A of ischemic muscle tissues derived from vehicle- or tPA-treated HL ischemic mice and nonischemic mice. Arrows indicate VEGFR-1+VEGF-A+ cells (bar = 20 μm). Images were acquired with a 40×/0.75 NA and a Carl Zeiss KS400 camera, and analyzed using Axio Vision (Carl Zeiss). (K-L) tPA or vehicle was injected intraperitoneally daily into tPA (K) or Plg (L) wild-type and deficient mice after induction of HL ischemia. Functional perfusion measurements of the collateral region were performed (n = 3 per group). A significant difference between tPA+/+ and tPA−/− mice treated with BSA-PBS is shown. If not otherwise mentioned, asterisks denote significant differences between BSA-PBS– and tPA-treated groups. *P < .05; **P < .001; values represent the mean ± SEM.

    Techniques Used: Injection, Imaging, Immunohistochemical staining, Staining, Derivative Assay, Functional Assay

    Transplantation of tPA-treated CD11b+ and VEGFR-1+ myeloid cells improve neoangiogenesis. (A-F) tPA- or BSA-PBS–mobilized PBMCs from GFP mice or carrier (no cells) were injected intravenously into HL ischemia–induced recipients. (A) Blood flow was determined. (B-F) Ischemic tissues of lower limbs isolated 15 days after injection and HL ischemia induction were stained with (B) H&E and (C) antibodies against CD31 for capillary density evaluation (n = 3 per group). Images were acquired with 20×/0.50 NA and a Carl Zeiss KS400 camera, and analyzed using Axio Vision (Carl Zeiss). (D-E) Within ischemic muscle tissues GFP+ donor cells (arrows) were quantified. Inserts depict a magnified view. Images were acquired with a 20×/0.50 NA and a 63×/1.3 oil objective using a Carl Zeiss KS400 camera. (E) Quantification of the number of GFP+ cells and GFP+ cells coexpressing CD11b in ischemic tissues (n = 8 per group). (F) CD11b (Mac-1) staining of lower limb ischemic tissue from mice receiving carrier injections, from mice that received a transplant with PBMCs, or from BSA-PBS–treated or tPA-treated donor GFP mice. Arrows indicate transplanted GFP+CD11b+ cells in ischemic tissues (bar = 20 μm). Images were acquired with 40×/0.75 NA and a Carl Zeiss KS400 camera, and analyzed using Axio Vision (Carl Zeiss). (G-H) PB-derived CD11b+ or CD11b− cells isolated from tPA- or BSA-PBS–treated donors were transplanted into HL ischemia–induced recipients for 3 days (n = 4 per group). (G) Blood flow was determined. (H) Capillary density was evaluated. (I-J) HL ischemic C57BL/6 mice were treated with tPA and coinjected with anti-CD11b or control monoclonal antibodies. (I) Blood flow was analyzed by laser Doppler analysis. (J) Capillary density was evaluated. (K) PB-derived VEGFR-1+ or VEGFR-1− cells, isolated from tPA- or BSA-PBS–treated donors, were injected into HL ischemia–induced recipients, and blood flow was examined (n = 4 per group). Single asterisk (P < .05) and double asterisk (P < .001) indicate a significant difference between the groups. Values represent mean ± SEM.
    Figure Legend Snippet: Transplantation of tPA-treated CD11b+ and VEGFR-1+ myeloid cells improve neoangiogenesis. (A-F) tPA- or BSA-PBS–mobilized PBMCs from GFP mice or carrier (no cells) were injected intravenously into HL ischemia–induced recipients. (A) Blood flow was determined. (B-F) Ischemic tissues of lower limbs isolated 15 days after injection and HL ischemia induction were stained with (B) H&E and (C) antibodies against CD31 for capillary density evaluation (n = 3 per group). Images were acquired with 20×/0.50 NA and a Carl Zeiss KS400 camera, and analyzed using Axio Vision (Carl Zeiss). (D-E) Within ischemic muscle tissues GFP+ donor cells (arrows) were quantified. Inserts depict a magnified view. Images were acquired with a 20×/0.50 NA and a 63×/1.3 oil objective using a Carl Zeiss KS400 camera. (E) Quantification of the number of GFP+ cells and GFP+ cells coexpressing CD11b in ischemic tissues (n = 8 per group). (F) CD11b (Mac-1) staining of lower limb ischemic tissue from mice receiving carrier injections, from mice that received a transplant with PBMCs, or from BSA-PBS–treated or tPA-treated donor GFP mice. Arrows indicate transplanted GFP+CD11b+ cells in ischemic tissues (bar = 20 μm). Images were acquired with 40×/0.75 NA and a Carl Zeiss KS400 camera, and analyzed using Axio Vision (Carl Zeiss). (G-H) PB-derived CD11b+ or CD11b− cells isolated from tPA- or BSA-PBS–treated donors were transplanted into HL ischemia–induced recipients for 3 days (n = 4 per group). (G) Blood flow was determined. (H) Capillary density was evaluated. (I-J) HL ischemic C57BL/6 mice were treated with tPA and coinjected with anti-CD11b or control monoclonal antibodies. (I) Blood flow was analyzed by laser Doppler analysis. (J) Capillary density was evaluated. (K) PB-derived VEGFR-1+ or VEGFR-1− cells, isolated from tPA- or BSA-PBS–treated donors, were injected into HL ischemia–induced recipients, and blood flow was examined (n = 4 per group). Single asterisk (P < .05) and double asterisk (P < .001) indicate a significant difference between the groups. Values represent mean ± SEM.

    Techniques Used: Transplantation Assay, Injection, Isolation, Staining, Derivative Assay

    Administration of recombinant VEGF-A partially rescues the angiogenic defect observed in Plg- and MMP-9–deficient mice. (A-B) Plasma VEGF-A levels were analyzed in tPA-treated and untreated HL-induced C57BL/6 (A) and chimeric (B) mice (n = 3 per group). Chimerism was achieved as follows: Lethally irradiated Plg+/+ or Plg−/− mice received a transplant with Plg+/+ or Plg−/− donor BM cells. Six weeks later, HL ischemia–induced chimeric mice were treated intraperitoneally with/without tPA. Asterisks and single dagger indicate a significant difference between tPA-treated and BSA-PBS–treated groups. (C) Immunohistochemical staining for CD11b and VEGF-A of ischemic muscle tissue derived from tPA-treated and vehicle-treated HL ischemic mice. Arrows indicate CD11b+ VEGF-A+ cells (bar = 25 μm). Images were acquired with 40×/0.75 NA and a Carl Zeiss KS400 camera, and analyzed using Axio Vision (Carl Zeiss). (D-H) HL-induced wild-type control (D-E,H), Plg−/− (F,H), and MMP-9−/− (G,H) mice were injected intraperitoneally daily with tPA, mVEGF-A, or BSA-PBS from day 0 until day 2. (D) Functional perfusion measurements of the collateral region with the use of a laser scan at the indicated time points in wild-type animals. Representative laser Doppler perfusion images are shown of the regions analyzed. Blood perfusion (red to yellow) was augmented in tPA-treated mice compared with vehicle-injected controls (green to blue = reduced perfusion signal). (E-G) Blood flow recovery was determined by laser Doppler perfusion imaging in the ischemic limbs (n = 3; *P < .05, **P < .001). (H) Capillary density was evaluated on CD31-stained sections 15 days after ligation (n = 3; **P < .001). Asterisks indicate a significant difference between the indicated groups. Error bars represent SEM.
    Figure Legend Snippet: Administration of recombinant VEGF-A partially rescues the angiogenic defect observed in Plg- and MMP-9–deficient mice. (A-B) Plasma VEGF-A levels were analyzed in tPA-treated and untreated HL-induced C57BL/6 (A) and chimeric (B) mice (n = 3 per group). Chimerism was achieved as follows: Lethally irradiated Plg+/+ or Plg−/− mice received a transplant with Plg+/+ or Plg−/− donor BM cells. Six weeks later, HL ischemia–induced chimeric mice were treated intraperitoneally with/without tPA. Asterisks and single dagger indicate a significant difference between tPA-treated and BSA-PBS–treated groups. (C) Immunohistochemical staining for CD11b and VEGF-A of ischemic muscle tissue derived from tPA-treated and vehicle-treated HL ischemic mice. Arrows indicate CD11b+ VEGF-A+ cells (bar = 25 μm). Images were acquired with 40×/0.75 NA and a Carl Zeiss KS400 camera, and analyzed using Axio Vision (Carl Zeiss). (D-H) HL-induced wild-type control (D-E,H), Plg−/− (F,H), and MMP-9−/− (G,H) mice were injected intraperitoneally daily with tPA, mVEGF-A, or BSA-PBS from day 0 until day 2. (D) Functional perfusion measurements of the collateral region with the use of a laser scan at the indicated time points in wild-type animals. Representative laser Doppler perfusion images are shown of the regions analyzed. Blood perfusion (red to yellow) was augmented in tPA-treated mice compared with vehicle-injected controls (green to blue = reduced perfusion signal). (E-G) Blood flow recovery was determined by laser Doppler perfusion imaging in the ischemic limbs (n = 3; *P < .05, **P < .001). (H) Capillary density was evaluated on CD31-stained sections 15 days after ligation (n = 3; **P < .001). Asterisks indicate a significant difference between the indicated groups. Error bars represent SEM.

    Techniques Used: Recombinant, Irradiation, Immunohistochemical staining, Staining, Derivative Assay, Injection, Functional Assay, Imaging, Ligation

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    Article Snippet: Afterward, the labelled sections were air-dried and exposed against tritium-sensitive films (Hyperfilm, Amersham, Braunschweig, Germany) together with plastic tritium standards of known radioactivity concentrations (Microscales®, Amersham) for 4–18 weeks. .. Each cell-body-stained section was scanned using a light microscope (Axioplan 2 imaging, Zeiss, Germany) equipped with a motor-operated stage controlled by the KS400 (Zeiss, Germany) image-analyzing system (version 3.0) and Axiovision (version 4.6), or a TISSUEscope HS (Huron, Canada), resulting in 8-bit images with an in-plane resolution of 1 μm per pixel (Fig. 1C). .. Using the KS400-system and in-house scripts in MatLab (The MathWorks, Natick, MA), digitized histological sections were converted into grey level index (GLI) images, which reflect the volume fraction of cell bodies (Schleicher et al., 2000) after adaptive thresholding (Palomero-Gallagher and Zilles, 2018).

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    Article Snippet: Sections were then fixed 5 min in a T-Max fixative (Kodak, two parts of T-Max and seven parts of distilled water), dehydrated in ascending grades of alcohol (70%, 96%, 100%) for 5 min in each dilution followed by two 5 min immersions in xylene before coverslipping with DePex mounting medium. .. Sections were scanned with a light microscope (Axioplan 2 imaging, Zeiss, Germany) equipped with a motor-operated stage controlled by the KS400 and Axiovision (Zeiss) image analysing systems applying a 6.3 ×1.25 objective (Planapo, Zeiss) and a CCD camera (Axiocam MRm, Zeiss). ..

    Imaging:

    Article Title: Multimodal mapping of macaque monkey somatosensory cortex.
    Article Snippet: Afterward, the labelled sections were air-dried and exposed against tritium-sensitive films (Hyperfilm, Amersham, Braunschweig, Germany) together with plastic tritium standards of known radioactivity concentrations (Microscales®, Amersham) for 4–18 weeks. .. Each cell-body-stained section was scanned using a light microscope (Axioplan 2 imaging, Zeiss, Germany) equipped with a motor-operated stage controlled by the KS400 (Zeiss, Germany) image-analyzing system (version 3.0) and Axiovision (version 4.6), or a TISSUEscope HS (Huron, Canada), resulting in 8-bit images with an in-plane resolution of 1 μm per pixel (Fig. 1C). .. Using the KS400-system and in-house scripts in MatLab (The MathWorks, Natick, MA), digitized histological sections were converted into grey level index (GLI) images, which reflect the volume fraction of cell bodies (Schleicher et al., 2000) after adaptive thresholding (Palomero-Gallagher and Zilles, 2018).

    Article Title: Cytoarchitectonic, receptor distribution and functional connectivity analyses of the macaque frontal lobe
    Article Snippet: Sections were then fixed 5 min in a T-Max fixative (Kodak, two parts of T-Max and seven parts of distilled water), dehydrated in ascending grades of alcohol (70%, 96%, 100%) for 5 min in each dilution followed by two 5 min immersions in xylene before coverslipping with DePex mounting medium. .. Sections were scanned with a light microscope (Axioplan 2 imaging, Zeiss, Germany) equipped with a motor-operated stage controlled by the KS400 and Axiovision (Zeiss) image analysing systems applying a 6.3 ×1.25 objective (Planapo, Zeiss) and a CCD camera (Axiocam MRm, Zeiss). ..

    Software:

    Article Title: Stromal Ki67 Expression Might be a Useful Marker for Distinguishing Fibroadenoma From Benign Phyllodes Tumor of the Breast.
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    Article Title: Phenotypic and molecular characterization of Prototheca wickerhamii from a Brazilian case of human systemic protothecosis.
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    Membrane:

    Article Title: Stromal Ki67 Expression Might be a Useful Marker for Distinguishing Fibroadenoma From Benign Phyllodes Tumor of the Breast.
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    Article Title: Magnetic recording medium having characterized vertical SFD, magnetic tape cartridge, and magnetic recording reproducing device
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    Article Title: Low dietary carbohydrate induces structural alterations in enterocytes of the chicken ileum.
    Article Snippet: Department of Science and Technology, Graduate School of Medicine, Science and Technology, Shinshu University, Kami-ina, Nagano, Japan Laboratory of Animal Functional Anatomy (LAFA), Faculty of Agriculture, Shinshu University, Kami-ina, Nagano, Japan Department of Agricultural and Life Sciences, Faculty of Agriculture, Shinshu University, Kami-ina, Nagano, Japan Laboratory of Animal Nutrition, Faculty of Agriculture, Iwate University, Morioka, Iwate, Japan

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    Nikon eclipse ti c2 confocal microscope
    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
    Eclipse Ti C2 Confocal Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c2+eclipse+ti+microscope/C2%2B/pm41330740-47-9-8
    Average 99 stars, based on 1 article reviews
    eclipse ti c2 confocal microscope - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

    99
    Nikon c2 eclipse ti microscope
    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon <t>Eclipse</t> <t>Ti-E</t> <t>C2+</t> <t>microscope.</t> Scale bar = 20 μm.
    C2 Eclipse Ti Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c2+eclipse+ti+microscope/C2%2B/pm41316927-51-11-10
    Average 99 stars, based on 1 article reviews
    c2 eclipse ti microscope - by Bioz Stars, 2026-10
    99/100 stars
      Buy from Supplier

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    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Article Snippet: After five days of static incubation at 30 °C, three-dimensional biofilm structures were observed using an Eclipse Ti-E C2+ confocal laser scanning microscope (Nikon) equipped with CFI Plan Apo VC 20x/1.2 and CFI Plan Apo VC 60x/1.2 WI objective lenses.

    Techniques: Staining, Mutagenesis, Fluorescence, Microscopy

    Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Journal: Biofilm

    Article Title: Inactivation of Cysteine Synthase CysK-A enhances flocculation, biofilm formation, and sensitivity to oxidative stress in Azospirillum brasilense Sp7

    doi: 10.1016/j.bioflm.2025.100335

    Figure Lengend Snippet: Colony and biofilm calcofluor staining assays. Production of exopolysaccharide (EPS) by the wild-type ( A. brasilense Sp7), the cysK-A mutant ( A. brasilense AR), and the complemented ( A . brasilense AR-pAB- cysK ) strains were visualized by staining with calcofluor, which binds to β-linked polysaccharides. ( A ) Colonies were grown for 5 days at 30 °C on solid LB medium (lacking NaCl) supplemented with Calcofluor (200 μg/mL). Images were captured under UV illumination to visualize fluorescence. Scale bar = 10 mm. ( B ) Confocal micrographs of biofilms grown for 5 days at 30 °C on agar-solidified Nfb∗ medium containing Calcofluor (85 μM). The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope. Scale bar = 20 μm.

    Article Snippet: The biofilms were observed with a Nikon Eclipse Ti-E C2+ microscope.

    Techniques: Staining, Mutagenesis, Fluorescence, Microscopy