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Upstate Biotechnology Inc dominant-negative mutant of c-src c-src dn
Dominant Negative Mutant Of C Src C Src Dn, supplied by Upstate Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c-src+dn/src+kinase/pm16920985-61-2-10
Average 90 stars, based on 1 article reviews
dominant-negative mutant of c-src c-src dn - by Bioz Stars, 2026-09
90/100 stars

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Article Title: Molecular chaperone TRAP1 regulates a metabolic switch between mitochondrial respiration and aerobic glycolysis
Article Snippet: Plasmid expressing WT c-Src was purchased from Upstate Biotechnology.

Article Title: β1 integrin-mediated signaling regulates MT1-MMP phosphorylation to promote tumor cell invasion.
Article Snippet: Control conditions for all experiments were cells treated with the same concentration of a nonspecific IgG (E7-s). cDNAs for human wild-type Src, constitutively active Src (Y529F), and dominant-negative Src (K296R/Y528F) were obtained from Upstate Biotechnology.

Article Title: Src-Mediated Phosphorylation of the Tyrosine Phosphatase PRL-3 Is Required for PRL-3 Promotion of Rho Activation, Motility and Invasion
Article Snippet: To generate SW480 cells stably expressing PRL-3 or mutants, coding sequences were cloned into pBABE-HAII. pUSE-Src was obtained from Upstate Biotechnology (Lake Placid, NY).

Article Title: Combinations of MEK inhibitors and Raf kinase inhibitors and uses thereof
Article Snippet: The ERK2 allele was subcloned from MAPK2/Erk2 cDNA (wild type) in pUSEamp (Upstate Biotechnology, Inc., Waltham, Mass.) into the vector pET21a (Novagen, Madison, Wis.) resulting in an N-terminal histidine-tagged mouse ERK2 allele.

Article Title: A Kinase-Independent Function of c-Src Mediates p130Cas Phosphorylation at the Serine-639 Site in Pressure Overloaded Myocardium
Article Snippet: Briefly, dominant negative c-Src cDNA (K295R, Y527F) and kinase active c-Src cDNA (Y527F) were obtained from Upstate Biotechnology as a cloning vector and subcloned into the cloning vector, pSP72 (Gibco-BRL).

Article Title: Derivatives of N-(arylamino) sulfonamides including polymorphs as inhibitors of MEK as well as compositions, methods of use and methods for preparing the same
Article Snippet: The ERK2 allele was subcloned from MAPK2/Erk2 cDNA (wild type) in pUSEamp (Upstate Biotechnology, Inc., Waltham, Mass.) into the vector pBT21a (Novagen, Madison, Wis.) resulting in an N-terminal histidine-tagged mouse ERK2 allele.

Dominant Negative Mutation:

Article Title: Treatment method using EGFR antibodies and SRC inhibitors and related formulations
Article Snippet: .. A dominant negative (DN), kinase dead Src construct (K296R/Y528F) was obtained from Upstate Biotechnology (Lake Placid, N.Y., USA). .. A Hind III fragment containing the DNSrc was sub-cloned into the pcDNA3.1/Hygro(+) vector obtained from Invitrogen Life Technologies (Carlsbad, Calif.) and the resulting construct transfected into U87MG.Δ2-7 cells b electroporation.

Construct:

Article Title: Treatment method using EGFR antibodies and SRC inhibitors and related formulations
Article Snippet: .. A dominant negative (DN), kinase dead Src construct (K296R/Y528F) was obtained from Upstate Biotechnology (Lake Placid, N.Y., USA). .. A Hind III fragment containing the DNSrc was sub-cloned into the pcDNA3.1/Hygro(+) vector obtained from Invitrogen Life Technologies (Carlsbad, Calif.) and the resulting construct transfected into U87MG.Δ2-7 cells b electroporation.



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