src (Proteintech)
Structured Review

Src, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+src/c-SRC+Antibody/pmc13040831-99-19-15
Average 93 stars, based on 43 article reviews
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1) Product Images from "Investigating the mechanisms by which bisphenol A affects osteoarthritis through a novel network toxicology framework and experimental validation"
Article Title: Investigating the mechanisms by which bisphenol A affects osteoarthritis through a novel network toxicology framework and experimental validation
Journal: BMC Pharmacology & Toxicology
doi: 10.1186/s40360-026-01108-0
Figure Legend Snippet: Experimental validation. ( A , C , E , G , I and K ) Relative expression of SRC, ESR1, EGFR, PTGS2, PPARG and HSP90AA1 via RT-qPCR. ( B , D , F , H , J and L ) Typical pictures and scores of the relative expression of SRC, ESR1, EGFR, PTGS2, PPARG and HSP90AA1 via IHC; 20×, Scale bar: 100 μm; 40×, Scale bar: 50 μm. ** P < 0.01, and *** P < 0.001
Techniques Used: Biomarker Discovery, Expressing, Quantitative RT-PCR
Related Articles
Incubation:Article Title: Investigating the effect of tenuigenin on LPS-induced HPMEC dysfunction by inhibiting SRC activation based on network pharmacology and molecular docking Article Snippet: Protein samples were mixed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protein Loading Buffer, denatured at 100°C for 10 min, separated on SurePAGETM Protein Precast Gels (Genscript, Nanjing, China) at 140 V for 1 h, and transferred onto polyvinylidene fluoride (PVDF) membranes (Beyotime). .. Membranes were subsequently closed by the 5% skim milk and incubated with anti-vascular endothelial cadherin (VE-cadherin) (1:1000, #2500, Cell signaling technology, Danvers, MA, USA), anti-Zona occludens 1 (ZO-1) (1:1000, #13663, Cell signaling technology), anti-phosphor-SRC (p-SRC) (1:5000, ab185617, Abcam, Cambridge, United Kingdom (UK)), Article Title: Mechanisms of Yiqi Huoxue Granule in Atherosclerosis Treatment: Insights from UPLC-Q-Exactive Orbitrap-MS Analysis, Network Pharmacology, Molecular Docking, and Experimental Verification Article Snippet: After mixing with loading buffer, protein samples were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred onto a polyvinylidene fluoride membrane (Cat. #IPVH00010, Millipore). .. The membrane was incubated with primary antibodies targeting Article Title: Investigating the mechanisms by which bisphenol A affects osteoarthritis through a novel network toxicology framework and experimental validation Article Snippet: .. The sections were incubated overnight at 4 °C with the following primary antibodies (all from Proteintech, diluted in PBS): Article Title: Mechanisms of Yiqi Huoxue Granule in Atherosclerosis Treatment: Insights from UPLC-Q-Exactive Orbitrap-MS Analysis, Network Pharmacology, Molecular Docking, and Experimental Verification Article Snippet: After mixing with loading buffer, protein samples were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred onto a polyvinylidene fluoride membrane (Cat. #IPVH00010, Millipore). .. The membrane was incubated with primary antibodies targeting Article Title: Investigating the effect of tenuigenin on LPS-induced HPMEC dysfunction by inhibiting SRC activation based on network pharmacology and molecular docking. Article Snippet: Protein samples were mixed with sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) protein Loading Buffer, denatured at 100°C for 10 min, separated on SurePAGETM Protein Precast Gels (Genscript, Nanjing, China) at 140 V for 1 h, and transferred onto polyvinylidene fluoride (PVDF) membranes (Beyotime). .. Membranes were subsequently closed by the 5% skim milk and incubated with anti-vascular endothelial cadherin (VE-cadherin) (1:1000, #2500, Cell signaling technology, Danvers, MA, USA), anti-Zona occludens 1 (ZO-1) (1:1000, #13663, Cell signaling technology), anti-phosphorSRC (p-SRC) (1:5000, ab185617, Abcam, Cambridge, United Kingdom (UK)), Membrane:Article Title: Mechanisms of Yiqi Huoxue Granule in Atherosclerosis Treatment: Insights from UPLC-Q-Exactive Orbitrap-MS Analysis, Network Pharmacology, Molecular Docking, and Experimental Verification Article Snippet: After mixing with loading buffer, protein samples were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred onto a polyvinylidene fluoride membrane (Cat. #IPVH00010, Millipore). .. The membrane was incubated with primary antibodies targeting Article Title: Mechanisms of Yiqi Huoxue Granule in Atherosclerosis Treatment: Insights from UPLC-Q-Exactive Orbitrap-MS Analysis, Network Pharmacology, Molecular Docking, and Experimental Verification Article Snippet: After mixing with loading buffer, protein samples were separated by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred onto a polyvinylidene fluoride membrane (Cat. #IPVH00010, Millipore). .. The membrane was incubated with primary antibodies targeting |
