anti src primary antibody (Santa Cruz Biotechnology)
Structured Review
Anti Src Primary Antibody, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 97/100, based on 1296 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+src/c-Src+Antibody/pm41872167-236-0-6
Average 97 stars, based on 1296 article reviews
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Incubation:Article Title: Cytoplasmic LIM domain only 2 enhances tumor endothelial cell migration through integrin β1-mediated focal adhesion signaling. Article Snippet: Equal amounts of protein were separated by SDS-polyacrylamide gel electrophoresis (PAGE) and transferred onto polyvinylidene fluoride (PVDF) membranes (BSP0861; Pall Corporation, USA). .. Membranes were blocked with 5% non-fat dry milk and incubated with primary antibodies at the indicated dilutions: anti-LMO2 (1:500) (NB110-78626; Novus Biologicals), anti-β-actin (1:10 000) (sc47778; Santa Cruz, USA), anti-ITGB1 (1:2 000) (A19072; ABclonal, South Korea), anti-p- AR TIC LE IN PR ES S VEGFR2 (Y1175; 1:1 000) (2478S; Cell Signaling, Danvers, MA, USA), anti-VEGFR2 (1:500) (2479S; Cell Signaling Technology, USA), anti-HIF1α (1:1 000) (ab51608; Abcam, Cambridge, England), anti-ITGA5 (1:1 000) (ab150361; Abcam), anti-Talin (1:1 000) (T3287; SigmaAldrich), anti-Paxillin (1:10 000) (ab32084; Abcam), anti-p-FAK (Y397, 1:500) (AP0302; ABclonal), anti-FAK (1:1 000) (A11195; ABclonal), anti-p-Src (Y416; 1:1 000) (2101S; Cell Signaling Technology), Article Title: Cytoplasmic LIM domain only 2 enhances tumor endothelial cell migration through integrin β1-mediated focal adhesion signaling Article Snippet: Equal amounts of protein were separated by SDS–polyacrylamide gel electrophoresis (PAGE) and transferred onto polyvinylidene fluoride (PVDF) membranes (BSP0861; Pall Corporation, USA). .. Membranes were blocked with 5% non-fat dry milk and incubated with primary antibodies at the indicated dilutions: anti-LMO2 (1:500) (NB110-78626; Novus Biologicals), anti-β-actin (1:10 000) (sc-47778; Santa Cruz, USA), anti-ITGB1 (1:2 000) (A19072; ABclonal, South Korea), anti-p-VEGFR2 (Y1175; 1:1 000) (2478S; Cell Signaling, Danvers, MA, USA), anti-VEGFR2 (1:500) (2479S; Cell Signaling Technology, USA), anti-HIF1α (1:1 000) (ab51608; Abcam, Cambridge, England), anti-ITGA5 (1:1 000) (ab150361; Abcam), anti-Talin (1:1 000) (T3287; Sigma-Aldrich), anti-Paxillin (1:10 000) (ab32084; Abcam), anti-p-FAK (Y397, 1:500) (AP0302; ABclonal), anti-FAK (1:1 000) (A11195; ABclonal), anti-p-Src (Y416; 1:1 000) (2101S; Cell Signaling Technology), Article Title: Extracellular vesicles shed by multidrug resistant cells contribute to the identification of SRC inhibitors as chemosensitizers in non-small cell lung cancer Article Snippet: Blocking was performed for at least 30 min in one of the following: 5% (w/v) non-fat dry milk in TBS-T (Tris-buffered saline, pH 7.4, with 0.1% Tween-20), 5% (w/v) bovine serum albumin (BSA) in TBS-T, or EveryBlot Blocking Buffer (Bio-Rad). .. Membranes were then incubated with the following primary antibodies for 90 min at RT or overnight at 4 °C: mouse anti-Alix (sc-53540), mouse anti-syntenin-1 (sc-100336), mouse anti-p-SRC (sc-81521), Blocking Assay:Article Title: Sustained monocyte activation by persistent challenges with either oxLDL or free cholesterol and underlying mechanisms. Article Snippet: .. After blocking with 10% goat serum, the samples were stained with Article Title: Sustained monocyte activation by persistent challenges with either oxLDL or free cholesterol and underlying mechanisms Article Snippet: .. After blocking with 10% goat serum, the samples were stained with Staining:Article Title: Sustained monocyte activation by persistent challenges with either oxLDL or free cholesterol and underlying mechanisms. Article Snippet: .. After blocking with 10% goat serum, the samples were stained with Article Title: Sustained monocyte activation by persistent challenges with either oxLDL or free cholesterol and underlying mechanisms Article Snippet: .. After blocking with 10% goat serum, the samples were stained with other:Article Title: Deciphering the STAT3-PXN positive feedback loop in GBM, IDH-wildtype: transcriptional regulation and inhibition of YB-1 ubiquitination. Article Snippet: |

