anti raf1 (Cell Signaling Technology Inc)
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Cell Signaling Technology Inc
anti raf1
Anti Raf1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 643 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+raf1/c-Raf+Antibody/pm41664072-84-28-30
Average 96 stars, based on 643 article reviews
Anti Raf1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 643 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+raf1/c-Raf+Antibody/pm41664072-84-28-30
Average 96 stars, based on 643 article reviews
anti raf1 - by Bioz Stars,
2026-11
96/100 stars
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Blocking Assay:Article Title: Bromodomain-containing protein 4 knockdown promotes neuronal ferroptosis in a mouse model of subarachnoid hemorrhage Article Snippet: The protein samples were then separated using the One-Step PAGE Gel Fast Preparation kit (Vazyme, Nanjin, Jiangsu, China; Cat# E302, E304) and transferred onto polyvinylidene difluoride membranes. .. After blocking at ambient temperature for 2 hours, the membranes were incubated with the following primary antibodies for at least 10 hours at 4°C: rabbit-anti-BRD4 (1:200, Abcam, Cambridge, UK; Cat# ab243862, RRID: AB_3096484), mouse anti-glutathione peroxidase 4 (GPX4; 1:1000, Proteintech, Wuhan, Hubei, China; Cat# 67763-1-Ig, RRID: AB_2909469), rabbit anti-solute carrier family 7 member 11 (SLC7A11; Proteintech; Cat# 26864-1-AP, RRID: AB_2880661), rabbit anti-coenzyme Q10 homolog B (CoQ10B; 1:1000, ThermoFisher Scientific; Cat# PA5-90284, RRID: AB_2806000), rabbit anti-SOSTM1/P62 (1:1000, Abclonal, Chongqing, China; Cat# A19700, RRID: AB_2862742), rabbit anti-microtubule-associated proteins 1A/1B light chain 3B (LC3B; 1:1000, Proteintech; Cat# 14600-1-AP, RRID: AB_2137737), rabbit anti-ferritin heavy chain (FTH1; 1:1000, Abmart, Shanghai, China; Cat# TD6278S, RRID: AB_3096423), Incubation:Article Title: Bromodomain-containing protein 4 knockdown promotes neuronal ferroptosis in a mouse model of subarachnoid hemorrhage Article Snippet: The protein samples were then separated using the One-Step PAGE Gel Fast Preparation kit (Vazyme, Nanjin, Jiangsu, China; Cat# E302, E304) and transferred onto polyvinylidene difluoride membranes. .. After blocking at ambient temperature for 2 hours, the membranes were incubated with the following primary antibodies for at least 10 hours at 4°C: rabbit-anti-BRD4 (1:200, Abcam, Cambridge, UK; Cat# ab243862, RRID: AB_3096484), mouse anti-glutathione peroxidase 4 (GPX4; 1:1000, Proteintech, Wuhan, Hubei, China; Cat# 67763-1-Ig, RRID: AB_2909469), rabbit anti-solute carrier family 7 member 11 (SLC7A11; Proteintech; Cat# 26864-1-AP, RRID: AB_2880661), rabbit anti-coenzyme Q10 homolog B (CoQ10B; 1:1000, ThermoFisher Scientific; Cat# PA5-90284, RRID: AB_2806000), rabbit anti-SOSTM1/P62 (1:1000, Abclonal, Chongqing, China; Cat# A19700, RRID: AB_2862742), rabbit anti-microtubule-associated proteins 1A/1B light chain 3B (LC3B; 1:1000, Proteintech; Cat# 14600-1-AP, RRID: AB_2137737), rabbit anti-ferritin heavy chain (FTH1; 1:1000, Abmart, Shanghai, China; Cat# TD6278S, RRID: AB_3096423), Article Title: GPR35 protects against reperfusion injury in ischemic stroke by binding with the CR2 domain of Raf1. Article Snippet: Mouse brain protein lysates were resolved using 10% SDS-PAGE and electrophoretically transferred onto Bio TraceTM NT nitrocellulose membranes (Pall, #66485). .. Membranes were blocked at room temperature for 2 h using bovine serum albumin and subsequently incubated overnight at 4°C with the indicated primary antibodies: anti-GPR35 (1:1000, Novus, NBP2-24640), Cell-Signaling:Article Title: Bromodomain-containing protein 4 knockdown promotes neuronal ferroptosis in a mouse model of subarachnoid hemorrhage Article Snippet: The protein samples were then separated using the One-Step PAGE Gel Fast Preparation kit (Vazyme, Nanjin, Jiangsu, China; Cat# E302, E304) and transferred onto polyvinylidene difluoride membranes. .. After blocking at ambient temperature for 2 hours, the membranes were incubated with the following primary antibodies for at least 10 hours at 4°C: rabbit-anti-BRD4 (1:200, Abcam, Cambridge, UK; Cat# ab243862, RRID: AB_3096484), mouse anti-glutathione peroxidase 4 (GPX4; 1:1000, Proteintech, Wuhan, Hubei, China; Cat# 67763-1-Ig, RRID: AB_2909469), rabbit anti-solute carrier family 7 member 11 (SLC7A11; Proteintech; Cat# 26864-1-AP, RRID: AB_2880661), rabbit anti-coenzyme Q10 homolog B (CoQ10B; 1:1000, ThermoFisher Scientific; Cat# PA5-90284, RRID: AB_2806000), rabbit anti-SOSTM1/P62 (1:1000, Abclonal, Chongqing, China; Cat# A19700, RRID: AB_2862742), rabbit anti-microtubule-associated proteins 1A/1B light chain 3B (LC3B; 1:1000, Proteintech; Cat# 14600-1-AP, RRID: AB_2137737), rabbit anti-ferritin heavy chain (FTH1; 1:1000, Abmart, Shanghai, China; Cat# TD6278S, RRID: AB_3096423), Article Title: Reversibly unlocking the blood–brain barrier: A study on microtubule dynamics modulation using gold nanoparticle-modified reduced graphene oxide Article Snippet: For protein analysis, brain tissues and HCMEC/D3 cells were lysed and separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using protein electrophoresis and blotting equipment (Bio-Rad, CA, USA). .. The primary antibodies were used: α -tubulin (Proteintech 11224-1-AP/66031-1-lg, IL, USA), β -tubulin (Proteintech 10094-1-AP, IL, USA), α -catenin (HuaAn Biotechnology ET1610-46, Hangzhou, China), β -catenin (Cell Signaling Technology 8480, MA, USA), VE-cadherin (Santa Cruz Biotechnology sc-9989, TX, USA), ZO-1 (Proteintech 21773-1-AP, IL, USA), claudin-5 (Abcam AB131259 , Cambridge, UK), occludin (Proteintech 27260-1-AP, IL, USA), tau (Santa Cruz Biotechnology sc-32274, TX, USA), MAP4 (Proteintech 11229-1-AP, IL, USA), p-GSK3 β (HuaAn Biotechnology ET1607-60, Hangzhou, China), GSK3 β (HuaAn Biotechnology ET1607-71, Hangzhou, China), crmp2 (HuaAn Biotechnology ER1802-45, Hangzhou, China), acetylated-tubulin (Proteintech 66200-1-lg, IL, USA), p-ERK (Proteintech 28733-1-AP, IL, USA), ERK (Proteintech 11257-1-AP, IL, USA), p-stathmin (Cell Signaling Technology 4191, MA, USA), stathmin (Proteintech 11157-1-AP, IL, USA), p-c-Raf (Cell Signaling Technology 9427, MA, USA), c-Raf ( Article Title: Proteomics- and BRET- screens identify SPRY2 as a Ras effector that impacts its membrane organization Article Snippet: Mouse monoclonal anti-B-Raf (F-7) , Santa Cruz Biotechnology , sc-5284; RRID: AB_626760. .. Planar Chromatography:Article Title: HIIT-induced lactate/GPR81 signaling with dual branches converging on ERK1/2 contributes to hippocampal synaptic remodeling and memory improvement Article Snippet: After a 15-min blocking step (PS108P, Epizyme, Shanghai, China), the samples were incubated with primary antibodies overnight at 4 °C. .. GPR81, PA5-114741, Thermo; AC (55067-1-AP, Proteintech), PKA (4782S, CST), RAP1 (2399S, CST), PKC (SC-80 Santa), PLC (14247S, CST), other:Article Title: Targeted therapy-induced chromosomal instability dictates mitotic dependency on Aurora Kinase A Article Snippet: Protein concentration was measured using the bicinchoninic acid assay (Thermo Fisher). |
50 , ![Pharmacological inhibition of the RAF1 pathway inhibits HCMV infection. ( A, B ) Regorafenib (Reg) or ( C, D ) sorafenib (Sor) treated ( A, C ) MRC5 or ( B, D ) HFF fibroblasts were treated with the indicated concentration of drugs or DMSO control at the time of infection. ( A–D ) Cells were infected at an MOI of 0.1 with AD169-GFP for 5 days, then fixed. GFP-positive cells were counted per area of the well and plotted against drug concentration [avg ± standard error of the mean (SEM), n = 3]. IC50 values were calculated. The dotted line indicates the average maximal GFP in untreated cells. ( E–H ) Fibroblasts were infected at an MOI of 3.0 in the presence of DMSO or 2.17 µM regorafenib or sorafenib added at the time of viral adsorption. UN is an untreated control. Viral titers from ( E ) MRC5 or ( F ) HFF cells were assessed by TCID50 (avg ± SEM, n = 3) 120 hpi. The dotted line indicates the limit of detection. In ( G ) MRC5 or ( H ) HFF cells, at 24 h post-infection (hpi), phosphorylation of MEK1/2 and ERK1/2 (arrows indicate doublet) was assessed by western blot ( n = 1).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5854/pmc11915854/pmc11915854__jvi.01866-24.f003.jpg)
![Inhibition of the RAF1 pathway negatively impacts viral DNA and protein accumulation. ( A–C ) MRC5 fibroblasts were treated with 2.17 µM of regorafenib or sorafenib or DMSO control at the time of infection with AD169 at an MOI of 3.0 for the indicated amount of time. ( A ) Viral DNA was quantified by RT-qPCR of the viral gene, IE1 [avg ± standard deviation (SD), n = 12]. ( B ) Samples in ( A ) were compared by area under the curve (AUC) to show the significance between treatments over the course of infection (avg ± SD, n = 12). Significance was established by a one-way analysis of variance test, followed by Tukey’s multiple-comparison test. ( C ) Viral protein expression levels at 24, 72, and 96 h post-infection (hpi) were assessed by western blot ( n = 2). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a loading control. ( D–G ) Quantification of western blots in ( C ) (avg ± standard error of the mean, n = 2) normalized to GAPDH and DMSO, 24 hpi sample for viral proteins ( D ) IE1, ( E ) IE2, ( F ) UL44, and ( G ) pp28.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5854/pmc11915854/pmc11915854__jvi.01866-24.f005.jpg)
![RAF1 shRNA-mediated knockdown inhibits HCMV infection. ( A–H ) Indicated shRNA guides were delivered via lentiviral delivery in HFF fibroblast cells. ( A–D ) RAF1 shRNA (shRAF1) were assessed for ( A ) knockdown efficiency in uninfected fibroblasts by RT-qPCR [avg ± standard error of the mean (SEM), n = 3 or 4], ( B ) protein expression was assessed via western blot ( n = 1), and ( C–D ) viral titer was measured by TCID50 (avg ± SEM, n = 4) at ( C ) an MOI of 3.0 at 120 h post-infection, or ( D ) an MOI of 0.01 at 10 days post-infection. ( E–F ) ARAF shRNA (shARAF) were assessed for ( E ) RNA levels via RT-qPCR and ( F ) viral titers via the GFP expression of cells infected at an MOI of 3.0 at 120 h post-infection. ( G–H ) BRAF shRNA (shBRAF) were assessed for ( G ) RNA levels via RT-qPCR and ( H ) viral titers via GFP expression of cells infected at an MOI of 3.0 at 120 h post-infection. ( A, E, G ) RNA levels were normalized to glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and shRNA empty vector (shEV) control in all RT-qPCR experiments. Significance was established by a one-way analysis of variance test, followed by Tukey’s multiple-comparison test.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5854/pmc11915854/pmc11915854__jvi.01866-24.f006.jpg)
![CRISPR-mediated knockout of RAF1 attenuates cell-to-cell spread of HCMV. ( A ) Knockout (KO) scores for CRISPR RAF1 KO cell lines generated. ( B–D ) MRC5 parental (par) and RAF1 polyclonal CRISPR knockout (poly KO) cells were infected at an MOI of 3.0 for ( B ) 96 h, and protein expression was assessed by western blot during mock ( M ) or HCMV ( H ) infection ( n = 1). UL26 is a control for successful viral infection, and glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a protein loading control. ( C ) At 72, 96, and 120 h post infection (hpi), viral titers were assessed by TCID50 [avg ± standard error of the mean (SEM), n = 2]. Significance is based on Student’s t -test at 120 hpi. ( D ) MRC5 (avg ± SEM, n = 72) and ( E ) HFF (avg ± SEM, n = 48), parental, and RAF1 polyclonal CRISPR knockout cell lines were infected with AD169-GFP at an MOI of 0.05 for 10 days. Infected cells per area of the well were counted via GFP expression, and significance was established by a one-way analysis of variance test, followed by Tukey’s multiple-comparison test.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5854/pmc11915854/pmc11915854__jvi.01866-24.f007.jpg)
![Pharmacological inhibition of RAF1 attenuates HCMV TB40/E cell-to-cell spread in fibroblasts and epithelial cells. ( A, B ) MRC5 fibroblasts or ( C ) ARPE19 epithelial cells were treated with 2.17 µM of regorafenib or sorafenib or DMSO control at the time of infection with ( A ) AD169-GFP or ( B, C ) TB40/E-mCherry at an MOI of 0.01. Viral spread was calculated by cells positive for ( A ) GFP or ( B ) mCherry on Day 12 in MRC5 cells or ( C ) mCherry on Days 15 and 30 in ARPE19 cells [avg ± standard error of the mean (SEM), n = 48]. Significance was established by a one-way analysis of variance test, followed by Tukey’s multiple-comparison test. ( D, E ) Mock-infected ARPE19 cells were treated with indicated concentrations of ( D ) regorafenib or ( E ) sorafenib at t = 0. Cells were fixed on Day 1, 5, or 11. Hoechst-stained nuclei were counted and plotted against time (avg ± SEM, n = 3).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5854/pmc11915854/pmc11915854__jvi.01866-24.f008.jpg)
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