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c 12005  (PromoCell)


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    PromoCell c 12005
    C 12005, supplied by PromoCell, used in various techniques. Bioz Stars score: 96/100, based on 388 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c+12005/Keratinocyte+Growth+Medium+2/pm40410272-246-5-4
    Average 96 stars, based on 388 article reviews
    c 12005 - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    other:

    Article Title: Topical application of urolithin A activates mitophagy and enhances mitochondrial function to promote skin longevity
    Article Snippet: Keratinocyte Growth Medium 2 (ready-to-use) , PromoCell , Cat#C-20011.

    Article Title: Targeting Cancer-Associated PCNA with AOH1996 Induces Mitotic Catastrophe and Enhances Cisplatin Therapy in Cervical Cancer
    Article Snippet: 191 Cell lines were either grown (37C, 5% CO2, humidified) in DMEM (Corning, 10-013-CV), supplemented with 192 10% FBS (VDR Life Sciences, 76308-942) (CaSki, C33a, HeLa, HeLa POLη) or maintained in Keratinocyte 193 Growth Medium 2 (PromoCell) supplemented with the Supplement Mix from PromoCell (HFKs, CaSki, 194 C33a, HeLa, HeLa POLη).

    Incubation:

    Article Title: Soluble bacterial lipopeptides suppress gasdermin D-associated IL-33 release in keratinocytes and atopic dermatitis in mice.
    Article Snippet: .. CFU/mL was adjusted by OD600nm to 10 7 CFU/mL, inoculated in 40 mL of Human Keratinocyte Growth Medium 2 AR TI CL E IN P RE SS (PromoCell) and incubated for 6 – 48 h at 37°C in a shaking incubator (200 rpm). .. CFU/mL was adjusted by OD600nm to 10 7 CFU/mL, inoculated in 40 mL of Human Keratinocyte Growth Medium 2 AR TI CL E IN P RE SS (PromoCell) and incubated for 6 – 48 h at 37°C in a shaking incubator (200 rpm).

    Article Title: Residual monomers and volatile leachable substances of conventional and CAD/CAM-fabricated denture base materials — GC-MS analysis and surface characterization
    Article Snippet: Specimens were UV-C-sterilized (254 nm) on autoclaved glass dishes for 20 min per side (HERAsafe KS 12; Life Technologies GmbH, Darmstadt, Germany). .. Subsequently, 14 specimens were incubated in a closed autoclaved glass bottle containing 11 ml of Keratinocyte Growth Medium 2 with supplements (KGM+; PromoCell, Heidelberg, Germany) on a rocker for 48 h at 37 °C to simulate clinically relevant extraction conditions. ..

    Cell Culture:

    Article Title: Keratinocyte Priming by Staphylococcus aureus Reduces HSV-1 Susceptibility.
    Article Snippet: .. Cells were cultured in keratinocyte growth medium 2 (Keratinocyte Basal Medium 2 plus supplements, PromoCell) without antibiotics at 37°C in a 5% CO2 atmosphere. ..

    Article Title: Radiometric and spectral characterization of multipanel planar light-emitting devices: a generalizable irradiance mapping approach for dose estimation
    Article Snippet: Primary normal human epidermal keratinocytes, isolated from the epidermis of juvenile foreskin or adult skin from pooled donors, were purchased from PromoCell (Heidelberg, Germany; Cat. No. C-12006). .. Cells were maintained in Keratinocyte Growth Medium 2 (KGM2; PromoCell), a serum-free cell culture medium for keratinocytes from the epidermis supplemented with 4 mL/L bovine pituitary extract, 0.125 ng/mL epidermal growth factor (recombinant human), 5 μg / mL insulin (recombinant human), 0.33 μg / mL hydrocortisone, 0.39 μg / mL epinephrine, 10 μg / mL transferrin (recombinant human), 0.06 mM CaCl 2 , 50 I.U./mL penicillin, and 50 μg / mL streptomycin. .. Cells were incubated at 37°C in the dark, in the presence of 5% CO 2 (Sanyo CO 2 incubator, Osaka, Japan) until the confluence reached 90%.

    Article Title: High-Capacity Dexamethasone-Loaded Poly(2-Oxazoline) Micelles for Ocular Surface Applications
    Article Snippet: The clinical efficacy of dexamethasone (DEX) in ocular therapy remains limited by its low aqueous solubility and restricted epithelial permeability.. To address these limitations, we developed high-capacity micellar formulations by a modified thin-film hydration method utilizing two poly(2-oxazoline) (POx)-based ABA triblock copolymers (A-pBuOx-A and A-pPentOx-A).. We achieved drug loading capacities of up to 33 wt.%.

    Glass:

    Article Title: Residual monomers and volatile leachable substances of conventional and CAD/CAM-fabricated denture base materials — GC-MS analysis and surface characterization
    Article Snippet: Specimens were UV-C-sterilized (254 nm) on autoclaved glass dishes for 20 min per side (HERAsafe KS 12; Life Technologies GmbH, Darmstadt, Germany). .. Subsequently, 14 specimens were incubated in a closed autoclaved glass bottle containing 11 ml of Keratinocyte Growth Medium 2 with supplements (KGM+; PromoCell, Heidelberg, Germany) on a rocker for 48 h at 37 °C to simulate clinically relevant extraction conditions. ..

    Extraction:

    Article Title: Residual monomers and volatile leachable substances of conventional and CAD/CAM-fabricated denture base materials — GC-MS analysis and surface characterization
    Article Snippet: Specimens were UV-C-sterilized (254 nm) on autoclaved glass dishes for 20 min per side (HERAsafe KS 12; Life Technologies GmbH, Darmstadt, Germany). .. Subsequently, 14 specimens were incubated in a closed autoclaved glass bottle containing 11 ml of Keratinocyte Growth Medium 2 with supplements (KGM+; PromoCell, Heidelberg, Germany) on a rocker for 48 h at 37 °C to simulate clinically relevant extraction conditions. ..

    Recombinant:

    Article Title: Radiometric and spectral characterization of multipanel planar light-emitting devices: a generalizable irradiance mapping approach for dose estimation
    Article Snippet: Primary normal human epidermal keratinocytes, isolated from the epidermis of juvenile foreskin or adult skin from pooled donors, were purchased from PromoCell (Heidelberg, Germany; Cat. No. C-12006). .. Cells were maintained in Keratinocyte Growth Medium 2 (KGM2; PromoCell), a serum-free cell culture medium for keratinocytes from the epidermis supplemented with 4 mL/L bovine pituitary extract, 0.125 ng/mL epidermal growth factor (recombinant human), 5 μg / mL insulin (recombinant human), 0.33 μg / mL hydrocortisone, 0.39 μg / mL epinephrine, 10 μg / mL transferrin (recombinant human), 0.06 mM CaCl 2 , 50 I.U./mL penicillin, and 50 μg / mL streptomycin. .. Cells were incubated at 37°C in the dark, in the presence of 5% CO 2 (Sanyo CO 2 incubator, Osaka, Japan) until the confluence reached 90%.



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    ( A ) Representative immunofluorescence images of P63, F-actin, and DAPI in both layers of the bilayer normal human epidermal <t>keratinocytes</t> (NHEK) cell culture system. DAPI image is z-depth color-coded. ( B ) Quantification of P63 intensity in Basal and Suprabasal cells (two-tailed unpaired t-test, p<0.0001, n=15 fields of view (FOV) containing 5-20 cells per FOV; 1 independent experiment). ( C ) Schematic of the two different gel constructs containing 100% collagen (Collagen) and 100% collagen gel coated with laminin (Col (LM-coated)). ( D ) Representative immunofluorescence z-stack cross sections of <t>NHEKs</t> cultured on each of the two gel systems. Cells were stained with F-actin and DAPI. ( E ) Representative immunofluorescence images of β-Catenin (CTNNB1) in culture on each gel type. Images are shown of the Suprabasal, Interlayer (between the Suprabasal and Basal), and the Basal cells. Immunofluorescence intensity is displayed using a 16-color scale. ( F ) Quantification of CTNNB1 intensity at Suprabasal junctions, Interlayer (between the Suprabasal and Basal), and Basal junctions in NHEKs cultured on the two gel systems. For each graph, intensity was normalized to the Collagen Gel Group (two-tailed unpaired t test (Suprabasal and Basal) and two-tailed Mann-Whitney test (Interlayer), p = 0.0046 (Suprabasal), 0.2213 (Interlayer), and p<0.0001 (Basal), n = 41-53 cells; 1 independent experiment). ( G ) Representative immunofluorescence images of Desmoplakin (DSP) in culture on each gel type. Images are shown of the Suprabasal, Interlayer (between the Suprabasal and Basal), and the Basal cells. Immunofluorescence intensity is represented as 16 colors. ( H ) Quantification of Desmoplakin intensity at Suprabasal junctions, Interlayer (between the Suprabasal and Basal), and Basal junctions in NHEKs cultured on the two gel systems. For each graph, intensity was normalized to the Collagen Gel Group (two-tailed unpaired t test (Suprabasal and Interlayer) and two-tailed Mann-Whitney test (Basal), p = 0.0042 (Suprabasal) and p<0.0001 (Interlayer and Basal), n = 40-50 cells; 1 independent experiment).
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    Image Search Results


    The ability to negate S. aureus -induced cytokine secretion from keratinocytes is a strain specific effect of M. luteus CFCS. (A, B) NHEK were treated with FSA or co-treated with FSA and skin bacterial CFCS for 24 h before quantifying IL-33 and TSLP in cell culture medium using ELISA. Stimulation of NHEK with FSA caused an increase in IL-33 and TSLP release. (B) Co-treatment with skin isolated M. luteus FAML CFCS negated FSA-induced release of IL-33 and TSLP. (C) Co-treatment with the M. luteus type strain NCTC 2665 had no effect on FSA-induced IL-33 and TSLP release. Data are expressed as mean ± SEM (n≥3). P values determined by one-way ANOVA *P ≤ 0.05 **P ≤ 0.01 ***P ≤ 0.001 ****P ≤ 0.0001 compared with FSA treated NHEK; ns, non-significant.

    Journal: Frontiers in Immunology

    Article Title: A skin isolate of Micrococcus luteus negates the Staphylococcus aureus- induced release of type 2 cytokines from keratinocytes

    doi: 10.3389/fimmu.2026.1711723

    Figure Lengend Snippet: The ability to negate S. aureus -induced cytokine secretion from keratinocytes is a strain specific effect of M. luteus CFCS. (A, B) NHEK were treated with FSA or co-treated with FSA and skin bacterial CFCS for 24 h before quantifying IL-33 and TSLP in cell culture medium using ELISA. Stimulation of NHEK with FSA caused an increase in IL-33 and TSLP release. (B) Co-treatment with skin isolated M. luteus FAML CFCS negated FSA-induced release of IL-33 and TSLP. (C) Co-treatment with the M. luteus type strain NCTC 2665 had no effect on FSA-induced IL-33 and TSLP release. Data are expressed as mean ± SEM (n≥3). P values determined by one-way ANOVA *P ≤ 0.05 **P ≤ 0.01 ***P ≤ 0.001 ****P ≤ 0.0001 compared with FSA treated NHEK; ns, non-significant.

    Article Snippet: Primary Normal Human Epidermal Keratinocytes (NHEK) (PromoCell, Heidelberg, Germany) were isolated from the epidermis of juvenile foreskin from pooled donors and grown in KGM supplemented with KGM SupplementMix (Promocell) at 37 ̊C in a humidified atmosphere of 5% CO 2 .

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Isolation

    The efficacious molecule secreted by M. luteus FAML is a putative protein. M. luteus FAML was cultured for 1, 2, 4, 6 and 24 h before harvesting CFCS. NHEK were co-cultured with FSA and M. luteus FAML CFCS for 24 h before measuring (A) TSLP and (B) IL-33 in cell culture medium using ELISA. (C) M . luteus FAML CFCS collected at 24 h lost activity against FSA-induced IL-33 and TSLP release in NHEK after heat treatment (HT) to 85°C. (D) Proteins within M. luteus FAML CFCS were precipitated using acetone, then reconstituted in cell culture medium before testing for activity using the same model. Activity was retained within the protein precipitate (PP). Data are expressed as mean ± SEM (n≥4). P values determined by one-way ANOVA *P ≤ 0.05 **P ≤ 0.01 ***P ≤ 0.001 ****P ≤ 0.0001 compared with FSA treated NHEK; ns, non-significant.

    Journal: Frontiers in Immunology

    Article Title: A skin isolate of Micrococcus luteus negates the Staphylococcus aureus- induced release of type 2 cytokines from keratinocytes

    doi: 10.3389/fimmu.2026.1711723

    Figure Lengend Snippet: The efficacious molecule secreted by M. luteus FAML is a putative protein. M. luteus FAML was cultured for 1, 2, 4, 6 and 24 h before harvesting CFCS. NHEK were co-cultured with FSA and M. luteus FAML CFCS for 24 h before measuring (A) TSLP and (B) IL-33 in cell culture medium using ELISA. (C) M . luteus FAML CFCS collected at 24 h lost activity against FSA-induced IL-33 and TSLP release in NHEK after heat treatment (HT) to 85°C. (D) Proteins within M. luteus FAML CFCS were precipitated using acetone, then reconstituted in cell culture medium before testing for activity using the same model. Activity was retained within the protein precipitate (PP). Data are expressed as mean ± SEM (n≥4). P values determined by one-way ANOVA *P ≤ 0.05 **P ≤ 0.01 ***P ≤ 0.001 ****P ≤ 0.0001 compared with FSA treated NHEK; ns, non-significant.

    Article Snippet: Primary Normal Human Epidermal Keratinocytes (NHEK) (PromoCell, Heidelberg, Germany) were isolated from the epidermis of juvenile foreskin from pooled donors and grown in KGM supplemented with KGM SupplementMix (Promocell) at 37 ̊C in a humidified atmosphere of 5% CO 2 .

    Techniques: Cell Culture, Enzyme-linked Immunosorbent Assay, Activity Assay

    Recombinant PADP negates FSA-induced IL-33 and TSLP release from NHEK. NHEK were co-cultured with FSA and rPADP for 24 h before measuring IL-33 and TSLP in the cell culture medium using ELISA. The rPADP negated FSA-induced IL-33 and TSLP release in NHEK. Data are expressed as mean ± SEM (n≥3). P values determined by one-way ANOVA *P ≤ 0.05 **P ≤ 0.01 ****P ≤ 0.0001 compared with FSA treated NHEK.

    Journal: Frontiers in Immunology

    Article Title: A skin isolate of Micrococcus luteus negates the Staphylococcus aureus- induced release of type 2 cytokines from keratinocytes

    doi: 10.3389/fimmu.2026.1711723

    Figure Lengend Snippet: Recombinant PADP negates FSA-induced IL-33 and TSLP release from NHEK. NHEK were co-cultured with FSA and rPADP for 24 h before measuring IL-33 and TSLP in the cell culture medium using ELISA. The rPADP negated FSA-induced IL-33 and TSLP release in NHEK. Data are expressed as mean ± SEM (n≥3). P values determined by one-way ANOVA *P ≤ 0.05 **P ≤ 0.01 ****P ≤ 0.0001 compared with FSA treated NHEK.

    Article Snippet: Primary Normal Human Epidermal Keratinocytes (NHEK) (PromoCell, Heidelberg, Germany) were isolated from the epidermis of juvenile foreskin from pooled donors and grown in KGM supplemented with KGM SupplementMix (Promocell) at 37 ̊C in a humidified atmosphere of 5% CO 2 .

    Techniques: Recombinant, Cell Culture, Enzyme-linked Immunosorbent Assay

    ( A ) Representative immunofluorescence images of P63, F-actin, and DAPI in both layers of the bilayer normal human epidermal keratinocytes (NHEK) cell culture system. DAPI image is z-depth color-coded. ( B ) Quantification of P63 intensity in Basal and Suprabasal cells (two-tailed unpaired t-test, p<0.0001, n=15 fields of view (FOV) containing 5-20 cells per FOV; 1 independent experiment). ( C ) Schematic of the two different gel constructs containing 100% collagen (Collagen) and 100% collagen gel coated with laminin (Col (LM-coated)). ( D ) Representative immunofluorescence z-stack cross sections of NHEKs cultured on each of the two gel systems. Cells were stained with F-actin and DAPI. ( E ) Representative immunofluorescence images of β-Catenin (CTNNB1) in culture on each gel type. Images are shown of the Suprabasal, Interlayer (between the Suprabasal and Basal), and the Basal cells. Immunofluorescence intensity is displayed using a 16-color scale. ( F ) Quantification of CTNNB1 intensity at Suprabasal junctions, Interlayer (between the Suprabasal and Basal), and Basal junctions in NHEKs cultured on the two gel systems. For each graph, intensity was normalized to the Collagen Gel Group (two-tailed unpaired t test (Suprabasal and Basal) and two-tailed Mann-Whitney test (Interlayer), p = 0.0046 (Suprabasal), 0.2213 (Interlayer), and p<0.0001 (Basal), n = 41-53 cells; 1 independent experiment). ( G ) Representative immunofluorescence images of Desmoplakin (DSP) in culture on each gel type. Images are shown of the Suprabasal, Interlayer (between the Suprabasal and Basal), and the Basal cells. Immunofluorescence intensity is represented as 16 colors. ( H ) Quantification of Desmoplakin intensity at Suprabasal junctions, Interlayer (between the Suprabasal and Basal), and Basal junctions in NHEKs cultured on the two gel systems. For each graph, intensity was normalized to the Collagen Gel Group (two-tailed unpaired t test (Suprabasal and Interlayer) and two-tailed Mann-Whitney test (Basal), p = 0.0042 (Suprabasal) and p<0.0001 (Interlayer and Basal), n = 40-50 cells; 1 independent experiment).

    Journal: bioRxiv

    Article Title: Epidermal Stem Cells Control Periderm Injury Repair via Matrix-Driven Specialization of Intercellular Junctions

    doi: 10.1101/2025.07.02.662640

    Figure Lengend Snippet: ( A ) Representative immunofluorescence images of P63, F-actin, and DAPI in both layers of the bilayer normal human epidermal keratinocytes (NHEK) cell culture system. DAPI image is z-depth color-coded. ( B ) Quantification of P63 intensity in Basal and Suprabasal cells (two-tailed unpaired t-test, p<0.0001, n=15 fields of view (FOV) containing 5-20 cells per FOV; 1 independent experiment). ( C ) Schematic of the two different gel constructs containing 100% collagen (Collagen) and 100% collagen gel coated with laminin (Col (LM-coated)). ( D ) Representative immunofluorescence z-stack cross sections of NHEKs cultured on each of the two gel systems. Cells were stained with F-actin and DAPI. ( E ) Representative immunofluorescence images of β-Catenin (CTNNB1) in culture on each gel type. Images are shown of the Suprabasal, Interlayer (between the Suprabasal and Basal), and the Basal cells. Immunofluorescence intensity is displayed using a 16-color scale. ( F ) Quantification of CTNNB1 intensity at Suprabasal junctions, Interlayer (between the Suprabasal and Basal), and Basal junctions in NHEKs cultured on the two gel systems. For each graph, intensity was normalized to the Collagen Gel Group (two-tailed unpaired t test (Suprabasal and Basal) and two-tailed Mann-Whitney test (Interlayer), p = 0.0046 (Suprabasal), 0.2213 (Interlayer), and p<0.0001 (Basal), n = 41-53 cells; 1 independent experiment). ( G ) Representative immunofluorescence images of Desmoplakin (DSP) in culture on each gel type. Images are shown of the Suprabasal, Interlayer (between the Suprabasal and Basal), and the Basal cells. Immunofluorescence intensity is represented as 16 colors. ( H ) Quantification of Desmoplakin intensity at Suprabasal junctions, Interlayer (between the Suprabasal and Basal), and Basal junctions in NHEKs cultured on the two gel systems. For each graph, intensity was normalized to the Collagen Gel Group (two-tailed unpaired t test (Suprabasal and Interlayer) and two-tailed Mann-Whitney test (Basal), p = 0.0042 (Suprabasal) and p<0.0001 (Interlayer and Basal), n = 40-50 cells; 1 independent experiment).

    Article Snippet: Normal human epidermal keratinocytes (NHEKs) were purchased from Promo Cell (C-12001) from a 4-year-old Caucasian male donor (Lot #: 494Z030.1).

    Techniques: Immunofluorescence, Cell Culture, Two Tailed Test, Construct, Staining, MANN-WHITNEY

    ( A ) Representative images of immunofluorescence staining specific to Human ECM proteins (rows), produced by the NHEKs when cultured on different animal-derived ECM scaffolds (columns). ( B-D ) Quantification of respective Human ECM proteins produced by NHEKs cultured on each of the gel systems (see ). (two-tailed Welch’s t test, p = 0.5393 (Collagen I), 0.2503 (Collagen IV), and 0.2227 (Laminin), n = 30-32 Field of views; 1 independent experiment). ( E ) Threshold images of interlayer Desmoplakin plaques in NHEKs cultured on each of the gel systems. ( F ) Quantification of Desmoplakin area between cell layers normalized to the collagen gel condition (Mann-Whitney test, p<0.0001, n= 936-1211 plaques; 1 independent experiment).

    Journal: bioRxiv

    Article Title: Epidermal Stem Cells Control Periderm Injury Repair via Matrix-Driven Specialization of Intercellular Junctions

    doi: 10.1101/2025.07.02.662640

    Figure Lengend Snippet: ( A ) Representative images of immunofluorescence staining specific to Human ECM proteins (rows), produced by the NHEKs when cultured on different animal-derived ECM scaffolds (columns). ( B-D ) Quantification of respective Human ECM proteins produced by NHEKs cultured on each of the gel systems (see ). (two-tailed Welch’s t test, p = 0.5393 (Collagen I), 0.2503 (Collagen IV), and 0.2227 (Laminin), n = 30-32 Field of views; 1 independent experiment). ( E ) Threshold images of interlayer Desmoplakin plaques in NHEKs cultured on each of the gel systems. ( F ) Quantification of Desmoplakin area between cell layers normalized to the collagen gel condition (Mann-Whitney test, p<0.0001, n= 936-1211 plaques; 1 independent experiment).

    Article Snippet: Normal human epidermal keratinocytes (NHEKs) were purchased from Promo Cell (C-12001) from a 4-year-old Caucasian male donor (Lot #: 494Z030.1).

    Techniques: Immunofluorescence, Staining, Produced, Cell Culture, Derivative Assay, Two Tailed Test, MANN-WHITNEY