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phosphate buffered saline d pbs  (Thermo Fisher)


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    Structured Review

    Thermo Fisher phosphate buffered saline d pbs
    Phosphate Buffered Saline D Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/buffer+d/PHOSPHATE+BUFFERED+SALINE+DPBS/pm41754955-102-12-15
    Average 99 stars, based on 1 article reviews
    phosphate buffered saline d pbs - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Saline:

    Article Title: Tubulin polyglutamylation modulates Golgi morphodynamics and neurite branching during neuronal morphogenesis
    Article Snippet: Cells were maintained and cultured as adherent monolayers at 37°C in a humidified incubator with 5% CO 2 under feeder-free conditions in StemFlex medium (A3349401; Gibco) on vitronectin (VTN-N)-coated 6-well plates (VTN-N Recombinant Human Protein, Truncated, A14700; Gibco). .. Plates were coated with vitronectin by diluting 10 μL vitronectin in 1 mL of 1x Dulbecco’s Phosphate-Buffered Saline (DPBS, 14190144; Gibco) per well and incubating for 2 hrs at 37°C. .. The hiPSCs were media changed every 1-2 days and were passaged upon reaching ∼80% confluency using ReLeSR (#100-0484; STEMCELL Technologies) according to manufacturer’s instructions in the presence of 0.5x ROCK inhibitor (RevitaCell Supplement, A2644501; Gibco).

    Article Title: Toxicological effects of acrylamide on oocyte maturation and subsequent embryonic development in a porcine model.
    Article Snippet: A 500 mM stock solution of ACR (A9099; purity ≥ 99%; Sigma-Aldrich) was prepared in distilled water and subsequently diluted with IVM medium to achieve final concentrations of 0, 100, 300, and 500 μM. .. To evaluate nuclear maturation after 44 h of IVM, COCs were transferred to a denuding solution composed of Dulbecco’s phosphatebuffered saline (DPBS; Gibco, Grand Island, NY, USA) supplemented with 100 μg/mL penicillin G, 75 μg/mL streptomycin sulfate, and 0.1% hyaluronidase. .. Following gentle pipetting to remove cumulus cells, denuded oocytes were examined under a microscope (Nikon Corp., Tokyo, Japan) and classified as immature (no polar body extrusion), metaphase II (MII; presence of polar body extrusion), or degenerate.

    Article Title: Development of a multi-targeted sulfonyl-bridged bisselenadiazole derivative as a potent EGFR/PI3K/AKT/mTOR modulator in lung adenocarcinoma
    Article Snippet: .. Attached cells were trypsinized with EDTA and resuspended in 1× Dulbecco's phosphate-buffered saline (DPBS, cat. no. ≠14190144, Gibco, ThermoFisher Scientific, USA). .. The cell suspensions were mixed with 0.4% trypan blue dye 1 : 1 (cat. no. 15250061, Gibco, Thermo Fisher Scientific, USA) and incubated for 3 min at room temperature at RT.

    Article Title: Bifunctional DNA multivalent structure integrating stable capture of urothelial carcinoma cells with CRISPR/Cas12a signal amplification for bladder cancer detection.
    Article Snippet: Noninvasive detection of bladder cancer remains challenging due to the limited analytical performance of current urine-based assays under realistic detection conditions.. Here, we report a linear programmable DNA nanostructure that integrates multivalent aptamer recognition with CRISPR/Cas12a signal transduction for detecting tumor-derived urinary exfoliated cells.. Assembled via hybridization chain reaction, the multivalent scaffold was shown to enhance ligand-cell binding stability under mechanically perturbed detection processes, thereby supporting mechanically stable biological recognition and yielding an approximately 14-fold increase in cellular binding affinity compared with monovalent aptamers.

    Article Title: Incorporation of imidazolium chitosan derivative yields scaffolds with enhanced antioxidant, antimicrobial, and immunomodulatory properties
    Article Snippet: The bacterial strains used in this study included: Escherichia coli (E. coli, ATCC 25922), Staphylococcus aureus (S. aureus, ATCC 29213), Enterococcus faecalis (E. faecalis ATCC 29212), and Pseudomonas aeruginosa (P. aeruginosa, ATCC 27853) all obtained from OxoidTM. .. For cell culture, Dulbecco's Phosphate-Buffered Saline (DPBS), Dulbecco's Modified Eagle Medium (DMEM), and Penicillin/Streptomycin (P/S) were obtained from Gibco. .. 3-(4,5-dimethylthiazol-2yl)-2, 5-diphenyl tetrazolium bromide (MTT) reagent was supplied by Sigma-Aldrich.

    Article Title: Superbase ionic liquid mediated solubilization of curcumin for improved bioavailability and anticancer efficacy.
    Article Snippet: HPLC-grade methanol (>99.9% of purity) was obtained from Fisher Scientific and KH2PO4 (> 99.5%) was purchased from Merck. .. For cell culture we used GIBCO Dulbecco’s phosphate buffered saline (DPBS), fetal (B) Curcumin [mTBNH][OAc] N N HN O O- N N N O O+ O OH O OH O HO O O O OH O HO Isomer bIsomer a Curcumin-enol Curcumin-keto (A ) .. AR TIC LE IN PR ES S ARTICLE IN PRESS bovine serum (FBS; approved by the EU, and sourced from South America), Dulbecco’s modified Eagle’s medium low glucose (DMEM–low Glc), Dulbecco’s modified Eagle’s medium high glucose (DMEM–high Glc), TrypLETM Express, GIBCO antibiotic/antimycotic solution (ATB) containing 10,000 units.mL−1 of penicillin and 10,000 mg.mL−1 of streptomycin, all of which were purchased from Thermo Fisher Scientific (Alfagene, PT).

    Article Title: Development of a multi-targeted sulfonyl-bridged bisselenadiazole derivative as a potent EGFR/PI3K/AKT/mTOR modulator in lung adenocarcinoma.
    Article Snippet: .. Attached cells were trypsinized with EDTA and resuspended in 1× Dulbecco's phosphatebuffered saline (DPBS, cat. no. s14190144, Gibco, RSC Adv., 2026, 16, 19903–19919 | 19905 O pe n A cc es s A rt ic le . ..

    Modification:

    Article Title: Bifunctional DNA multivalent structure integrating stable capture of urothelial carcinoma cells with CRISPR/Cas12a signal amplification for bladder cancer detection.
    Article Snippet: Noninvasive detection of bladder cancer remains challenging due to the limited analytical performance of current urine-based assays under realistic detection conditions.. Here, we report a linear programmable DNA nanostructure that integrates multivalent aptamer recognition with CRISPR/Cas12a signal transduction for detecting tumor-derived urinary exfoliated cells.. Assembled via hybridization chain reaction, the multivalent scaffold was shown to enhance ligand-cell binding stability under mechanically perturbed detection processes, thereby supporting mechanically stable biological recognition and yielding an approximately 14-fold increase in cellular binding affinity compared with monovalent aptamers.

    Article Title: Incorporation of imidazolium chitosan derivative yields scaffolds with enhanced antioxidant, antimicrobial, and immunomodulatory properties
    Article Snippet: The bacterial strains used in this study included: Escherichia coli (E. coli, ATCC 25922), Staphylococcus aureus (S. aureus, ATCC 29213), Enterococcus faecalis (E. faecalis ATCC 29212), and Pseudomonas aeruginosa (P. aeruginosa, ATCC 27853) all obtained from OxoidTM. .. For cell culture, Dulbecco's Phosphate-Buffered Saline (DPBS), Dulbecco's Modified Eagle Medium (DMEM), and Penicillin/Streptomycin (P/S) were obtained from Gibco. .. 3-(4,5-dimethylthiazol-2yl)-2, 5-diphenyl tetrazolium bromide (MTT) reagent was supplied by Sigma-Aldrich.

    Cell Culture:

    Article Title: Incorporation of imidazolium chitosan derivative yields scaffolds with enhanced antioxidant, antimicrobial, and immunomodulatory properties
    Article Snippet: The bacterial strains used in this study included: Escherichia coli (E. coli, ATCC 25922), Staphylococcus aureus (S. aureus, ATCC 29213), Enterococcus faecalis (E. faecalis ATCC 29212), and Pseudomonas aeruginosa (P. aeruginosa, ATCC 27853) all obtained from OxoidTM. .. For cell culture, Dulbecco's Phosphate-Buffered Saline (DPBS), Dulbecco's Modified Eagle Medium (DMEM), and Penicillin/Streptomycin (P/S) were obtained from Gibco. .. 3-(4,5-dimethylthiazol-2yl)-2, 5-diphenyl tetrazolium bromide (MTT) reagent was supplied by Sigma-Aldrich.

    Article Title: Superbase ionic liquid mediated solubilization of curcumin for improved bioavailability and anticancer efficacy.
    Article Snippet: HPLC-grade methanol (>99.9% of purity) was obtained from Fisher Scientific and KH2PO4 (> 99.5%) was purchased from Merck. .. For cell culture we used GIBCO Dulbecco’s phosphate buffered saline (DPBS), fetal (B) Curcumin [mTBNH][OAc] N N HN O O- N N N O O+ O OH O OH O HO O O O OH O HO Isomer bIsomer a Curcumin-enol Curcumin-keto (A ) .. AR TIC LE IN PR ES S ARTICLE IN PRESS bovine serum (FBS; approved by the EU, and sourced from South America), Dulbecco’s modified Eagle’s medium low glucose (DMEM–low Glc), Dulbecco’s modified Eagle’s medium high glucose (DMEM–high Glc), TrypLETM Express, GIBCO antibiotic/antimycotic solution (ATB) containing 10,000 units.mL−1 of penicillin and 10,000 mg.mL−1 of streptomycin, all of which were purchased from Thermo Fisher Scientific (Alfagene, PT).

    Plasmid Preparation:

    Article Title: Unique DNA impacts on transient Expi293 TM PRO expression for enhancing biotherapeutic protein production
    Article Snippet: The transfected Expi293FTM was fed with Expi293TM Transfection Enhancer-1 (Thermo Fisher Scientific, Cat. #100013864) and ExpiFectamineTM 293 Transfection Enhancer-2 (Thermo Fisher Scientific, Cat. #A14350-02), while Expi293 TM PRO were fed with Expi293 TM PRO Enhancer (Thermo Fisher Scientific, Cat. #A40000966) and Expi293 TM PRO Feed (Thermo Fisher Scientific, Cat. #A40001356-02) after 20 h post-transfection and incubated in incubators at 37 °C, 120 rpm, 8% CO 2 for 6 days. .. At 20 h post-transfection for the plasmid DNA encoding the surface-display molecule, the transfected cells were collected and washed with Dulbecco’s phosphate-buffered saline (DPBS) (Thermo Fisher Scientific, Cat. #14-040-133) and labeled with goat anti-human IgG Fc, PE-conjugated (Thermo Fisher Scientific, Cat. #12-4998-82) at a 1:100 dilution with DPBS. .. Doublet cells were excluded, and PE % positive cells and mean fluorescence intensity were acquired by a BD LSRFortessaTM cell analyzer (Beckon Dickinson, Franklin Lakes, NJ, United States) with FACSDiva software (Beckon Dickinson).

    Transfection:

    Article Title: Unique DNA impacts on transient Expi293 TM PRO expression for enhancing biotherapeutic protein production
    Article Snippet: The transfected Expi293FTM was fed with Expi293TM Transfection Enhancer-1 (Thermo Fisher Scientific, Cat. #100013864) and ExpiFectamineTM 293 Transfection Enhancer-2 (Thermo Fisher Scientific, Cat. #A14350-02), while Expi293 TM PRO were fed with Expi293 TM PRO Enhancer (Thermo Fisher Scientific, Cat. #A40000966) and Expi293 TM PRO Feed (Thermo Fisher Scientific, Cat. #A40001356-02) after 20 h post-transfection and incubated in incubators at 37 °C, 120 rpm, 8% CO 2 for 6 days. .. At 20 h post-transfection for the plasmid DNA encoding the surface-display molecule, the transfected cells were collected and washed with Dulbecco’s phosphate-buffered saline (DPBS) (Thermo Fisher Scientific, Cat. #14-040-133) and labeled with goat anti-human IgG Fc, PE-conjugated (Thermo Fisher Scientific, Cat. #12-4998-82) at a 1:100 dilution with DPBS. .. Doublet cells were excluded, and PE % positive cells and mean fluorescence intensity were acquired by a BD LSRFortessaTM cell analyzer (Beckon Dickinson, Franklin Lakes, NJ, United States) with FACSDiva software (Beckon Dickinson).

    Labeling:

    Article Title: Unique DNA impacts on transient Expi293 TM PRO expression for enhancing biotherapeutic protein production
    Article Snippet: The transfected Expi293FTM was fed with Expi293TM Transfection Enhancer-1 (Thermo Fisher Scientific, Cat. #100013864) and ExpiFectamineTM 293 Transfection Enhancer-2 (Thermo Fisher Scientific, Cat. #A14350-02), while Expi293 TM PRO were fed with Expi293 TM PRO Enhancer (Thermo Fisher Scientific, Cat. #A40000966) and Expi293 TM PRO Feed (Thermo Fisher Scientific, Cat. #A40001356-02) after 20 h post-transfection and incubated in incubators at 37 °C, 120 rpm, 8% CO 2 for 6 days. .. At 20 h post-transfection for the plasmid DNA encoding the surface-display molecule, the transfected cells were collected and washed with Dulbecco’s phosphate-buffered saline (DPBS) (Thermo Fisher Scientific, Cat. #14-040-133) and labeled with goat anti-human IgG Fc, PE-conjugated (Thermo Fisher Scientific, Cat. #12-4998-82) at a 1:100 dilution with DPBS. .. Doublet cells were excluded, and PE % positive cells and mean fluorescence intensity were acquired by a BD LSRFortessaTM cell analyzer (Beckon Dickinson, Franklin Lakes, NJ, United States) with FACSDiva software (Beckon Dickinson).



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