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NimbleGen Systems GmbH hybridization buffer/hybridization component
Hybridization Buffer/Hybridization Component, supplied by NimbleGen Systems GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/buffer+components/hybridization+buffer+hybridization+component/pmc07396748-119-24-32
Average 90 stars, based on 1 article reviews
hybridization buffer/hybridization component - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Salinomycin triggers endoplasmic reticulum stress through ATP2A3 upregulation in PC-3 cells
Article Snippet: Human 12 × 135 K Gene Expression Arrays (Roche NimbleGen) were hybridized at 42 °C for 16 to 20 h with 4 μg of Cy3 labeled ds-cDNA in NimbleGen hybridization buffer/hybridization component in a hybridization chamber (Hybridization System-NimbleGen Systems, Inc., Madison, WI, USA).

Article Title: Neural Stem Cell Transplantation Promotes Functional Recovery from Traumatic Brain Injury via Brain Derived Neurotrophic Factor-Mediated Neuroplasticity.
Article Snippet: Traumatic brain injury (TBI) induces cognitive impairments, motor and behavioral deficits.. Previous evidences have suggested that neural stem cell (NSC) transplantation could facilitate functional recovery from brain insults, but their underlying mechanisms remains to be elucidated.. Here, we established TBI model by an electromagnetic-controlled cortical impact device in the rats.

Article Title: Whole-Genome Expression Analysis and Signal Pathway Screening of Synovium-Derived Mesenchymal Stromal Cells in Rheumatoid Arthritis
Article Snippet: Microarrays were hybridized with 4 μ g of Cy3-labeled ds-cDNA in NimbleGen Hybridization Buffer/Hybridization Component A in a hybridization chamber (Hybridization System-NimbleGen Systems, Inc.) at 42°C over 16 to 20 h. After hybridization, the 14 microarrays were washed in an ozone-free environment using a NimbleGen Wash Buffer kit (NimbleGen Systems, Inc.).

Article Title: DNA methylation status of imprinted H19 and KvDMR1 genes in human placentas after conception using assisted reproductive technology
Article Snippet: The labeled DNA and chip were hybridized at 42 °C for 16–20 h. The hybridization process was performed in a hybridization cassette using the Nimblegen hybridization buffer/hybridization component A (Hybridisation System - Nimblegen Systems, Inc., Madison, WI, USA).

Article Title: Integrated analyses of a major histocompatibility complex, methylation and transcribed ultra-conserved regions in systemic lupus erythematosus.
Article Snippet: The microarrays were hybridized in Nimblegen hybridization buffer/hybridization component A in a hybridization chamber (Hybridization System-Nimblegen Systems, Inc., Madison, WI, USA).

Article Title: Characterization of microRNA expression profiling in peripheral blood lymphocytes in rats with experimental autoimmune uveitis.
Article Snippet: Objective and design We aimed to investigate the alterations of microRNA (miRNA) genomics in peripheral blood lymphocytes in experimental autoimmune uveitis (EAU) rats versus control samples.. Materials/methods Six Lewis rats received interphotoreceptor retinoid-binding protein (IRBP) emulsion to induce EAU.. On day 12, peripheral blood lymphocytes were isolated, and total RNAs were extracted.

Hybridization:

Article Title: Long non-coding RNA AK001903 regulates tumor progression in cervical cancer
Article Snippet: Next, 100 pmol of deoxynucleoside triphosphates and 100 U of the Klenow fragment (New England Biolabs Inc.) were added and the mixture incubated at 37°C for 2 h. The reaction was stopped by 0.5 mol/l EDTA (0.1 times the volume of the previous mixture), and the labeled ds-cDNA was purified by isopropanol/ethanol precipitation. .. Microarrays were hybridized at 42°C for 16–20 h with 4 μg of Cy3 labelled ds-cDNA in hybridization buffer/hybridization component A (NimbleGen Systems, Inc.) in a hybridization chamber (hybridization system; NimbleGen Systems, Inc.). .. Washing was performed three times using the SeqCap EZ Hybridization and Wash kit (cat. no. 05634261001; NimbleGen Systems, Inc.).



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Image Search Results


Components are represented on sagittal, coronal, and transversal slices of a high-resolution template of the marmoset brain . Brain coordinates of the presented perspective are reported in the left bottom corner of each panel. Components include higher-order visual (HOV), subcortical-visual (SUV), somatomotor (SOM), default mode (DMN), occipitoparietal (OP), primary visual (PVIS), basal ganglia (BG), motor-cerebellar (MCE), and auditory (AUD) networks. Components were selected based on spatiotemporal features and visual inspection. Color indicates voxel-wise z-scores, thresholded at p < 0.5 (posterior probability that a voxel belongs to the active distribution rather than noise).

Journal: Communications Biology

Article Title: Mapping functional homologies between human and marmoset brain networks using movie-driven ultra-high field fMRI

doi: 10.1038/s42003-025-09241-y

Figure Lengend Snippet: Components are represented on sagittal, coronal, and transversal slices of a high-resolution template of the marmoset brain . Brain coordinates of the presented perspective are reported in the left bottom corner of each panel. Components include higher-order visual (HOV), subcortical-visual (SUV), somatomotor (SOM), default mode (DMN), occipitoparietal (OP), primary visual (PVIS), basal ganglia (BG), motor-cerebellar (MCE), and auditory (AUD) networks. Components were selected based on spatiotemporal features and visual inspection. Color indicates voxel-wise z-scores, thresholded at p < 0.5 (posterior probability that a voxel belongs to the active distribution rather than noise).

Article Snippet: A second, largely independent cluster contained the SOM network, which showed weak correlation with the motor-cerebellar component (MCE; r = 0.14) and moderate to strong anticorrelations with all other functional components ( r = –0.32 to –0.96).

Techniques: Process/Product Development