blasticidin (Thermo Fisher)
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Thermo Fisher
blasticidin
Blasticidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/buffer+a/Blasticidin+S+hydrochloride%2C+98%2B%25%2C+in+1M+HEPES+buffer+soln/pm42259351-86-15-21
Average 97 stars, based on 1 article reviews
Blasticidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/buffer+a/Blasticidin+S+hydrochloride%2C+98%2B%25%2C+in+1M+HEPES+buffer+soln/pm42259351-86-15-21
Average 97 stars, based on 1 article reviews
blasticidin - by Bioz Stars,
2026-10
97/100 stars
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Cell Culture:Article Title: Genomic landscape of drug binding and pharmacogenetic variation across diverse populations using SNPdrug3D. Article Snippet: .. HEK293 Flp-In, T-ReX cell line was purchased from Invitrogen (#R78007) and cultured in DMEM containing 10% FBS (Excell), 1% NEAA, 1% Penicillin/Streptomycin (Gibco), 15g/ml Transduction:Article Title: Inflammatory cytokines induce new cancer dependencies. Article Snippet: Collins K. Cheruiyot, Sarah Y. Kim, Juan Dubrot , Sarah K. Lane-Reticker, Alex Miranda, Ashwin V. Kammula , Jonathan J. Perera , Peter P. Du , Cun Lan Chuong, Rachel A. Fetterman, Nelson H. Knudsen , Aiping Jiang , Juliette S. M. T. Suermondt, Lomax F. Pass, Cong Fu, Meng-Ju Wu , Lei Shi , Seth Anderson , Audrey J. Muscato , Omar I. Avila , Ian C. Kohnle, Emily A. Kessler , Hans W. Pope, Sarah G. Noel , Kira E. Olander , Jooho Chung , Kayla J. Colvin, Nabeel Bardeesy, Kathleen B. Yates & Robert T. Manguso Article Title: Mapping ST3GAL Transferase Specificities in the Glycosylation Landscape of N/TERT-1 Keratinocytes Using Glycogenomics and Mass Spectrometry. Article Snippet: Virus-containing supernatants were collected 48-72 h post-transfection, filtered (0.45 μm pore size), mixed 1:1 with fresh complete K-FSM, supplemented with polybrene (1:1000, Sigma), and the N/TERT-1 keratinocytes were transduced overnight. .. Selection of KO populations was initiated between 48 and 96 h after transduction, using either 5 μg/ml Selection:Article Title: Mapping ST3GAL Transferase Specificities in the Glycosylation Landscape of N/TERT-1 Keratinocytes Using Glycogenomics and Mass Spectrometry. Article Snippet: Virus-containing supernatants were collected 48-72 h post-transfection, filtered (0.45 μm pore size), mixed 1:1 with fresh complete K-FSM, supplemented with polybrene (1:1000, Sigma), and the N/TERT-1 keratinocytes were transduced overnight. .. Selection of KO populations was initiated between 48 and 96 h after transduction, using either 5 μg/ml Article Title: ETS-guided iPSC-endothelial models recapitulate malaria pathogenesis. Article Snippet: These entry vectors were © The Author(s) EMBO Molecular Medicine 15 then recombined into a piggyBac destination vector containing an antibiotic resistance cassette using the MultiSite Gateway Pro system (Thermo Fisher) (Woltjen et al, 2009). .. The resulting constructs were electroporated into IMR90-4 iPSCs using the Amaxa 4D-Nucleofector protocol (Lonza), followed by antibiotic selection with puromycin (Merck), geneticin (Gibco) and Subculturing Assay:Article Title: Mapping ST3GAL Transferase Specificities in the Glycosylation Landscape of N/TERT-1 Keratinocytes Using Glycogenomics and Mass Spectrometry. Article Snippet: Virus-containing supernatants were collected 48-72 h post-transfection, filtered (0.45 μm pore size), mixed 1:1 with fresh complete K-FSM, supplemented with polybrene (1:1000, Sigma), and the N/TERT-1 keratinocytes were transduced overnight. .. Selection of KO populations was initiated between 48 and 96 h after transduction, using either 5 μg/ml Stable Transfection:Article Title: Widespread circulation of Alongshan virus in Austria and serological evidence for infection in humans: a nationwide molecular and serological observational study. Article Snippet: Stable S2 cell lines were established by calcium phosphate transfection with the respective expression plasmid and a blasticidin resistance plasmid, following the manufacturer’s protocol (Invitrogen Calcium Phosphate Transfection Kit, Thermo Fisher Scientific). .. Stably transfected S2 cells were then selected by supplementing the culture medium with 25 μg/mL Transfection:Article Title: Widespread circulation of Alongshan virus in Austria and serological evidence for infection in humans: a nationwide molecular and serological observational study. Article Snippet: Stable S2 cell lines were established by calcium phosphate transfection with the respective expression plasmid and a blasticidin resistance plasmid, following the manufacturer’s protocol (Invitrogen Calcium Phosphate Transfection Kit, Thermo Fisher Scientific). .. Stably transfected S2 cells were then selected by supplementing the culture medium with 25 μg/mL Article Title: Cells, islets, and organoids that evade immune detection and autoimmunity, methods of production and use thereof Article Snippet: .. Seventy-two (72) hours after transfection, INS-1 cells were treated with 5 μg/ml Construct:Article Title: ETS-guided iPSC-endothelial models recapitulate malaria pathogenesis. 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The resulting constructs were electroporated into IMR90-4 iPSCs using the Amaxa 4D-Nucleofector protocol (Lonza), followed by antibiotic selection with puromycin (Merck), geneticin (Gibco) and Expressing:Article Title: Cells, islets, and organoids that evade immune detection and autoimmunity, methods of production and use thereof Article Snippet: .. Seventy-two (72) hours after transfection, INS-1 cells were treated with 5 μg/ml Article Title: A conserved mechanism for regulation of mtDNA copy number in eukaryotes Article Snippet: Viral supernatant was filter sterilised and added to SLC25A39KO3 and the control clone cells for 48 hours after addition of 6μg/mL polybrene (Merck, TR-1003). .. Cells with integrated plasmid were selected using 10ug/ml Plasmid Preparation:Article Title: A conserved mechanism for regulation of mtDNA copy number in eukaryotes Article Snippet: Viral supernatant was filter sterilised and added to SLC25A39KO3 and the control clone cells for 48 hours after addition of 6μg/mL polybrene (Merck, TR-1003). .. Cells with integrated plasmid were selected using 10ug/ml Western Blot:Article Title: A conserved mechanism for regulation of mtDNA copy number in eukaryotes Article Snippet: Viral supernatant was filter sterilised and added to SLC25A39KO3 and the control clone cells for 48 hours after addition of 6μg/mL polybrene (Merck, TR-1003). .. Cells with integrated plasmid were selected using 10ug/ml |