dapi staining solution (Beyotime)

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Beyotime dapi staining solution

CuPaeNs bound to FSCN1. A) Bubble chart of GO enrichment analysis top 10 of cellular component after CuPaeNs treatment. B) <t>Immunofluorescence</t> <t>staining</t> of B16 cells in different groups. <t>DAPI</t> (blue), FSCN1 (green), and F‐actin (red). C) Molecular simulation of CuPaeNs. D) Computational model of active sites related to the potential key target of FSCN1 with paeonol, a single paeonol‐Cu 2+ unit, and CuPaeNs. The residues of the ligand‐binding domain proteins, involved in hydrogen bonds, were illustrated and marked with yellow dotted lines. E) Molecular simulation illustrating the binding affinity and detailed intermolecular binding interactions of FSCN1 with paeonol, a single Cu 2+ ‐paeonol unit, and CuPaeNs, focusing on main hydrogen bonds and hydrophobic interactions. F) Isothermal titration calorimetry result demonstrating the direct binding of CuPaeNs to FSCN1. G) CLSM images showing intracellular FSCN1 binding with CuPaeNs in B16 cells (scale bar = 20 µm) and H) responding pixel intensity plot. I) Assessment of CuPaeNs's impact on FSCN1's actin‐bundling activity using the Co‐IP assay. (J) Protein expression of FSCN1, F‐actin, and β‐actin with B16 cells in different groups, along with quantitative analysis ( n = 3). Statistically significant differences are indicated with their respective p ‐values ( *** p < 0.001).

Dapi Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 2060 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/buffer+a+itc/DAPI+Staining+Solution/pmc13042531-249-2-44
Average 99 stars, based on 2060 article reviews

dapi staining solution - by Bioz Stars, 2026-10

99/100 stars

Images

1) Product Images from "Beyond Catalytic Therapy: Copper‐Paeonol Nanozymes Disrupt Fascin‐Mediated Actin Bundling to Suppress Tumor Growth and Metastasis"

Article Title: Beyond Catalytic Therapy: Copper‐Paeonol Nanozymes Disrupt Fascin‐Mediated Actin Bundling to Suppress Tumor Growth and Metastasis

Journal: Advanced Science

doi: 10.1002/advs.202512186

CuPaeNs bound to FSCN1. A) Bubble chart of GO enrichment analysis top 10 of cellular component after CuPaeNs treatment. B) Immunofluorescence staining of B16 cells in different groups. DAPI (blue), FSCN1 (green), and F‐actin (red). C) Molecular simulation of CuPaeNs. D) Computational model of active sites related to the potential key target of FSCN1 with paeonol, a single paeonol‐Cu 2+ unit, and CuPaeNs. The residues of the ligand‐binding domain proteins, involved in hydrogen bonds, were illustrated and marked with yellow dotted lines. E) Molecular simulation illustrating the binding affinity and detailed intermolecular binding interactions of FSCN1 with paeonol, a single Cu 2+ ‐paeonol unit, and CuPaeNs, focusing on main hydrogen bonds and hydrophobic interactions. F) Isothermal titration calorimetry result demonstrating the direct binding of CuPaeNs to FSCN1. G) CLSM images showing intracellular FSCN1 binding with CuPaeNs in B16 cells (scale bar = 20 µm) and H) responding pixel intensity plot. I) Assessment of CuPaeNs's impact on FSCN1's actin‐bundling activity using the Co‐IP assay. (J) Protein expression of FSCN1, F‐actin, and β‐actin with B16 cells in different groups, along with quantitative analysis ( n = 3). Statistically significant differences are indicated with their respective p ‐values ( *** p < 0.001).

Figure Legend Snippet: CuPaeNs bound to FSCN1. A) Bubble chart of GO enrichment analysis top 10 of cellular component after CuPaeNs treatment. B) Immunofluorescence staining of B16 cells in different groups. DAPI (blue), FSCN1 (green), and F‐actin (red). C) Molecular simulation of CuPaeNs. D) Computational model of active sites related to the potential key target of FSCN1 with paeonol, a single paeonol‐Cu 2+ unit, and CuPaeNs. The residues of the ligand‐binding domain proteins, involved in hydrogen bonds, were illustrated and marked with yellow dotted lines. E) Molecular simulation illustrating the binding affinity and detailed intermolecular binding interactions of FSCN1 with paeonol, a single Cu 2+ ‐paeonol unit, and CuPaeNs, focusing on main hydrogen bonds and hydrophobic interactions. F) Isothermal titration calorimetry result demonstrating the direct binding of CuPaeNs to FSCN1. G) CLSM images showing intracellular FSCN1 binding with CuPaeNs in B16 cells (scale bar = 20 µm) and H) responding pixel intensity plot. I) Assessment of CuPaeNs's impact on FSCN1's actin‐bundling activity using the Co‐IP assay. (J) Protein expression of FSCN1, F‐actin, and β‐actin with B16 cells in different groups, along with quantitative analysis ( n = 3). Statistically significant differences are indicated with their respective p ‐values ( *** p < 0.001).

Techniques Used: Immunofluorescence, Staining, Ligand Binding Assay, Binding Assay, Isothermal Titration Calorimetry, Activity Assay, Co-Immunoprecipitation Assay, Expressing

Bicinchoninic Acid Protein Assay:

Article Title: The host protein SSR4 mediates PRRSV-induced endoplasmic reticulum stress via interaction with Nsp2.
Article Snippet: The following primary antibodies were used: anti-β-actin (Proteintech, 66009-1-Ig), anti-Calnexin (Proteintech, 10427-2-AP), anti-SSR1 (Proteintech, 10583-1-AP), anti-SSR2 (Proteintech, 10278-1-AP), anti-SSR3 (Proteintech, 30851-1-AP), anti-SSR4 (Proteintech, 11655-2-AP), anti-ATF4 (Proteintech, 10835-1-AP), anti-EDEM1 (Proteintech, 26226-1-AP), anti-GRP78 (Beyotime, AF0171), anti-ATF6 (Proteintech, 24169-1-AP), anti-Phospho-eIF2 alpha (Cell Signaling Technology, #3398), anti-eIF2 alpha (Cell Signaling Technol ogy, #5324), anti-Phospho-IRE1 (HUABIO, HA721980), anti-IRE1 (HUABIO, HA723225), anti-Puromycin (ABclonal, A21205), monoclonal anti-PRRSV N protein (generated in-house), and monoclonal anti-PRRSV Nsp2 (generated in-house). .. Key commercial reagents included tunicamycin (Beyotime, SC0393), DTT (Solarbio, 3483-12-3), 4-phenyl butyric acid (MCE, HY-A0281), MK-28 (AbMole, M56016), puromycin (MCE, HY-B1743), cycloheximide (MCE, HY-12320), BCA Protein Assay Kit (Thermo Fisher, 23225), Lipo fectamine 3000 (Thermo Fisher, L3000015), Lipofectamine RNAiMAX (Thermo Fisher, 13778075), ReverTra Ace qPCR RT Kit (TOYOBO, NLQ-101), KOD qPCR SYBR Mix (TOYOBO, QKD-201T), tunicamycin (MedChemExpress, HY-A0098), RIPA lysis buffer (Beyotime, P0013C), DAPI staining solution (Beyotime, C1006), and polyethylenimine (PEI; Yeasen). ..

Article Title: Development of perilla essential oil-based nanoemulsions as anti-psoriatic and dermal delivery platforms for topical therapy
Article Snippet: PEG-40 stearyl ether (S40) was obtained from Croda GmbH (Nett et al., Germany). .. DAPI staining solution, RIPA buffer, hematoxylin and eosin (H&E) staining kit, and bicinchoninic acid (BCA) protein assay kits were obtained from Beyotime Biotechnology (Shanghai, China). .. Antibodies against Ki67, TNF-α, IL-6, IL-1β, IL-17 and a one-step TUNEL assay kit were from Servicebio (Wuhan, China).

Real-time Polymerase Chain Reaction:

Lysis:

Staining:

Article Title: A photodynamically activated nanoplatform relieves glucose-driven immunosuppression to potentiate STING immunotherapy in triple-negative breast cancer
Article Snippet: Pre-screened foetal bovine serum (FBP-C520) was procured from Haixing Biosciences. .. DAPI Staining Solution, Cell Viability/Cytotoxicity assay kit, Cell Cycle and Apoptosis analysis kit, Reactive Oxygen Species (ROS) assay kit, and Mitochondrial Membrane Potential assay kit with JC-1 were all purchased from Beyotime, Inc. .. Cell Counting Kit-8 was obtained from TargetMol, USA.

Article Title: Gastrodin alleviates neuronal damage in epileptic cell models by targeting P2RY12 to inhibit microglial hyperactivation
Article Snippet: .. Then, DAPI staining solution (Beyotime, China, C1005, 1 μg/mL) was added, and the cells were incubated for 5 minutes to stain the nuclei. .. After washing with PBS, the images were acquired using a confocal microscope (Zeiss LSM880).

Article Title: Targeted delivery of lupeol via hyaluronic acid-modified ZIF-8 for anti-hepatic fibrosis therapy
Article Snippet: Quantitative analysis was performed using flow cytometry (Beckman Coulter, USA). .. A DAPI Staining Solution (Beyotime Biotechnology, Shanghai, China) was used to perform the cell apoptosis shape assay on LX-2 cells according to the manufacturer's instructions. .. Cells were seeded into six-well plates at a density of 1.0 × 10 6 cells per well and cultured for 24 h. The next day, cells were treated with different media containing Lupeol, Lupeol@ZIF-8 and HA/Lupeol@ZIF-8 (60 μM lupeol), with blank and model groups included as controls, and incubated for an additional 24 h. For qualitative analysis, DAPI solution was added 24 h after treatment, and fluorescence signals were recorded using a fluorescence microscope (Olympus, Tokyo, Japan).

Article Title: Geometry-driven immunomodulation in 3D-printed bioceramics: Negative curvature promotes macrophage M2 polarization via Ras-MAPK/HIF-1α signaling for vascularized osteogenesis
Article Snippet: The cells types utilized in this study included: hBMSCs (No. 7500, ScienCell, USA.), Raw264.7 (CL-0190, Procell system, China), HUVECs (No. 8000, ScienCell, USA.), rBMSCs (MUBMX-01001, OriCell, USA.) and MS1(CL-0162, Procell system, China). .. The reagents used in the experiment included: H-DMEM(11965118, Gibco, USA.), α-DMEM medium(12571063, Gibco, USA.), TritonX-100(ST1723, Beyotime, China), 4 % paraformaldehyde (BL539A, Biosharp, China),FBS(A5256701, Gibco, USA.),ECM medium (Science Cell, USA.),and DAPI staining solution (C1006, Beyotime, China),BCIP/NBT(C3206, Beyotime, China), reactive oxygen species kit (S0033S, Beyotime, China), BSA (B2064, ≥98 %, Sigma-Aldrich, USA.),CD31 antibody (ab28364, Abcam, USA.), secondary anti-IGg (ab175773, Alexa Fluor® 680, Abcam, USA.), Phalloidin-iFluor 488(ab176753, Abcam, USA.), CCR7(AF5293, Bioss, China), CD206 (bsm-60761R, Bioss, China), iNOS (bs-22924R, Bioss, China), RIPA (P0013, Beyotime, China), p-ERK1/2 (AF3687, Affinity, USA.) and ERK1/2 (#AF0155, Affinity, USA), luminol detection reagent (sc-2048, Santa Cruz, USA.), GAPDH (Cat#KC-5G5, Kangchen Biotechnology, China), Trizol(15596026CN, Invitrogen, USA.), DEPC(R0601, Thermo Scientific, USA.), TBST(R017R.0000, Thermo Scientific, USA.), HIF-1a(GTX127309, GeneTex, USA.), β-Tubulin(10094-1-AP, Proteintech, UK) Adezmapimod (SB 203580, MCE, USA.) medium and Paclitaxel (99.88 %, HY-B0015R, MCE), ELISA Arg-1(E-EL-M3092, ELabSci@, China), TNF-α (E-EL-M3063, ELabSci@, China), OPN(22952-1-AP, Proteintech, UK.), F4/80 (GB11027-100, Servicebio, China) Alkaline phosphatase activity kit (P0321S, Beyotime, China), Matrigel (CLS356234, Corning, USA), Microfill MV120 (Flow tech, USA), EDTA(17892, Thermo Scientific, USA.) xylene(X112051, AR,99 %, Aladdin, China), ethanol (107-21-1, AR,99 %, Aladdin, China). .. All primers were designed and synthesized by Genema (Shanghai) Co., Ltd. 5.2.Fabrication of high-throughput array cell culture chip (HCGC) with Gaussian curvature gradient distribution. (1) Constructing models with different Gaussian curvatures: the multipipe of Grasshopper in Rhinocero software is used to regulate Gaussian curvature of the truss in the diamond framework in .

Article Title: Activation of the TGR5/cAMP/PKA/CREB axis in cholangiocytes mediates epithelial-mesenchymal transition and fibrosis in hepatolithiasis.
Article Snippet: On the following day, cells were washed three times with PBS (5 minutes each), then 313 incubated at 37°C for 30 minutes with fluorescent secondary antibodies (e.g., Alexa Fluor 488/594 314 conjugates; Invitrogen, 1:500). .. After additional PBS washes, DAPI staining solution (Beyotime, 315 Cat# C1002, 1 μg/mL) was added and incubated in the dark for 3 minutes. ..

Article Title: Gastrodin alleviates neuronal damage in epileptic cell models by targeting P2RY12 to inhibit microglial hyperactivation.
Article Snippet: .. Then, DAPI staining solution (Beyotime, China, C1005, 1 μg/mL) was added, and the cells were incubated for 5 minutes to stain the nuclei. .. After washing with PBS, the images were acquired using a confocal microscope (Zeiss LSM880).

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dapi staining solution (Beyotime)

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Images

1) Product Images from "Beyond Catalytic Therapy: Copper‐Paeonol Nanozymes Disrupt Fascin‐Mediated Actin Bundling to Suppress Tumor Growth and Metastasis"

Related Articles

Bicinchoninic Acid Protein Assay:

Real-time Polymerase Chain Reaction:

Lysis:

Staining:

ROS Assay:

Membrane:

Incubation:

Enzyme-linked Immunosorbent Assay:

Activity Assay:

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