buffer 3 (IonPath Inc)

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IonPath Inc buffer 3
Buffer 3, supplied by IonPath Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/buffer+4/buffer+4/us12020920-453-10-14
Average 90 stars, based on 1 article reviews

buffer 3 - by Bioz Stars, 2026-10

90/100 stars

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Article Title: Source-detector synchronization in multiplexed secondary ion mass spectrometry
Article Snippet: .. To perform this step, the polymer can be suspended in Buffer 1 (available from IONpath, Menlo Park, Calif.), and the metal element of interest added. .. The mixture can then be incubated for 45 minutes at 37° C., and subsequently transferred to a filter (e.g., a 3 kDa molecular-weight cut off (MWCO) Amicon® spin filter, available from MilliporeSigma, Burlington, Mass.) that retains the polymer and enables removal of unbound metal tags by washing the polymer twice with Buffer 1.

Article Title: Source-detector synchronization in multiplexed secondary ion mass spectrometry
Article Snippet: In the third step, the metal-tagged polymer and reduced antibody can be combined and incubated for 60-90 minutes at 37° C. to conjugate the polymer to the antibody. .. Following this incubation, the mixture can be washed three times with Buffer 4 (available from IONpath, Menlo Park, CA) using the same 50 kDa MWCO filter, which retains the conjugated antibody and allows removal of unbound polymer. .. The concentration of the metal-tagged antibody can be determined by measuring optical absorbance at 280 nm (e.g., using a NanoDrop spectrofluorometer, available from ThermoFisher Scientific, Waltham, MA) for a solution of the metal-tagged antibody in Buffer 5 (available from IONpath, Menlo Park, CA), diluted to a concentration of between 200 μg/mL and 500 μg/mL, and stored at a temperature of 4° C. In some embodiments, for preparation of samples consisting of arrays of cells, as shown in FIG. 7, cells in suspension can be augmented with surface marker antibodies and incubated at room temperature for approximately 30 minutes.

Article Title: Source-detector synchronization in multiplexed secondary ion mass spectrometry
Article Snippet: .. To perform this step, the polymer can be suspended in Buffer 1 (available from IONpath, Menlo Park, CA), and the metal element of interest added. .. The mixture can then be incubated for 45 minutes at 37° C. and subsequently transferred to a filter (e.g., a 3 kDa molecular-weight cut off (MWCO) Amicon® spin filter, available from MilliporeSigma, Burlington, MA) that retains the polymer and enables removal of unbound metal tags by washing the polymer twice with Buffer 1.

Article Title: Synthesis, Characterization, and Applications of a Superior Dendrimer-Based Polymer for Multiplexed Ion Beam Imaging Time-of-Flight Analysis.
Article Snippet: High-dimensional single-cell mass spectrometric imaging techniques such as multiplexed ion beam imaging by timeof-flight mass spectrometry (MIBI-TOF), imaging mass cytometry (IMC), and flow cytometry-based CyTOF utilize antibodies conjugated to linear metal-chelating polymers.. Here, we report on the synthesis and characterization of a dendrimer-based polymer and its utilization in tissue imaging using MIBI-TOF.. We compared the staining performance in FFPE tissue of antibodies for lineage-specific immune proteins (CD20, CD3, CD45, FoxP3) that were conjugated with dendrimer or linear polymer.

Incubation:

Article Title: Source-detector synchronization in multiplexed secondary ion mass spectrometry
Article Snippet: Next, the antibody can be incubated with a reducing agent, such as 4 mM tris(2-carboxyethyl)phosphine (TCEP) for 30 minutes at 37° C. to partially reduce the antibody and expose sulfhydryl residues for conjugation with the maleimide-containing polymer. .. Following the incubation, the antibody can be washed twice with Buffer 3 (available from IONpath, Menlo Park, CA) using the same 50 kDa MWCO filter to remove the TCEP and prevent further reduction to the antibody. .. In the third step, the metal-tagged polymer and reduced antibody can be combined and incubated for 60-90 minutes at 37° C. to conjugate the polymer to the antibody.

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