Recombinant:Article Title: Myocardial Fibrosis and Cardiomyopathy Risk: A Genetic Link in the MESA
Article Snippet: Circ Heart Fail.. 2023;16:e010262.. DOI: 10.1161/CIRCHEARTFAILURE.122.010262 September 2023 Correspondence to: Naveen L. Pereira, MD, Department of Cardiovascular Medicine, Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, 200 First St SW, Rochester, MN 55905, Email pereira.naveen@mayo.edu; or Joao A. C. Lima, MBA, MD, Division of Cardiology, Department of Medicine, Johns Hopkins Hospital, 600 N Wolfe St, Baltimore, MD, 21287-0409, Email jlima@jhmi.edu *M. Shabani and M. Wang contributed equally.
Article Title: Chemical Synthesis and Antifibrotic Properties of (±)-Cochlearol T, (±)-Ganocochlearin A, and (±)-Cochlearol Y
Article Snippet: .. Recombinant human TGF-β1 (human cell-expressed) protein (#7754-BH-005) was purchased from R&D System/bio-techne (Minneapolis, MN) and reconstituted in Reconstitution Buffer 4 (BSA/HCl) (R&D System, #RB04). ..
Article Title: Pharmacological Characterization of Zasocitinib (TAK-279): An Oral, Highly Selective, and Potent Allosteric TYK2 Inhibitor.
Article Snippet: .. Buffers and antibodies for flow cytometric analysis of signal transducer and activator of transcription (STAT) phosphorylation were obtained from BD Biosciences (San Diego, CA), BioLegend (San Diego, CA), and eBioscience (San Diego, CA), Miltenyi Biotec (Westphalia, Germany), R&D Systems (Minneapolis, MN), Rockland Immunochemicals (Limerick, PA), and Thermo Fisher Scientific as follows: BD Phosflow Lyse/Fix buffer (catalog number 558049), BD Phosflow Perm Buffer III (catalog number 558050), BD Brilliant Stain Buffer (catalog number 566349), BD BSA stain buffer (catalog number 554657), Phycoerythrin-conjugated mouse antihuman CD42a antibody (catalog number 558819), FITC-conjugated mouse antihuman CD3 antibody (catalog number 561807), and Alexa Fluor 647econjugated mouse antihuman Stat5 pY694 antibody (catalog number 562076) from BD Biosciences; phycoerythrin-conjugated mouse anti-STAT4 (pY693) (catalog number 941204), Alexa Fluor 647econjugated mouse antihuman CD161 antibody (catalog number 339910), Alexa Fluor 647econjugated mouse antihuman CD61 antibody (catalog number 336408), Brilliant Violet 421econjugated mouse antihuman STAT3 Phospho (Tyr705) antibody (catalog number 651010), Brilliant Violet 605econjugated mouse antihuman CD62P antibody (catalog number 304920), Alexa Fluor 647econjugated mouse antihuman STAT4 Phospho (Tyr693) antibody (catalog number 941204), APCCy7econjugated mouse antihuman CD41 antibody (catalog number 303716), and Fc receptor blocking solution (catalog number 422302) from BioLegend; Fc receptor binding inhibitor polyclonal antibody (catalog number 14-9161-71) from eBioscience; AutoMACS running buffer (catalog number 130-091-221) from Miltenyi Biotec; reconstitution buffer (catalog number RB05 or RB02), Rockland Immunochemicals BSA 30% solution (lot number 49664), BD BSA stain buffer (catalog number BSA-30), and FACS buffer comprising PBS þ 1% BSA from R&D Systems; and Hanks’ Balanced Salt Solution (catalog number 14025092), FITC-conjugated mouse antihuman STAT3 Phospho (Tyr705) antibody (catalog number 11-9033-42), and recombinant human ILs from Thermo Fisher Scientific. .. Type 1 IFN and thrombopoietin were obtained as follows: recombinant human IL-2 (catalog number PHC00267, Life Technologies, Carlsbad, CA), recombinant human IL-12 (catalog number 200-12, PeproTech US, Cranbury, NJ), recombinant human IL-18 (catalog number 9124-IL-050, R&D Systems), HumanKine recombinant human IL-23 (catalog number HZ-1254, Proteintech, Rosemont, IL), recombinant human type 1 IFN (catalog number 11200-1, PBL Assay Science, Piscataway, NJ), and recombinant human thrombopoietin (catalog number 300-18, Thermo Fisher Scientific).
Sterility:Article Title: Myocardial Fibrosis and Cardiomyopathy Risk: A Genetic Link in the MESA
Article Snippet: Circ Heart Fail.. 2023;16:e010262.. DOI: 10.1161/CIRCHEARTFAILURE.122.010262 September 2023 Correspondence to: Naveen L. Pereira, MD, Department of Cardiovascular Medicine, Department of Molecular Pharmacology and Experimental Therapeutics, Mayo Clinic, 200 First St SW, Rochester, MN 55905, Email pereira.naveen@mayo.edu; or Joao A. C. Lima, MBA, MD, Division of Cardiology, Department of Medicine, Johns Hopkins Hospital, 600 N Wolfe St, Baltimore, MD, 21287-0409, Email jlima@jhmi.edu *M. Shabani and M. Wang contributed equally.
Phospho-proteomics:Article Title: Pharmacological Characterization of Zasocitinib (TAK-279): An Oral, Highly Selective, and Potent Allosteric TYK2 Inhibitor.
Article Snippet: .. Buffers and antibodies for flow cytometric analysis of signal transducer and activator of transcription (STAT) phosphorylation were obtained from BD Biosciences (San Diego, CA), BioLegend (San Diego, CA), and eBioscience (San Diego, CA), Miltenyi Biotec (Westphalia, Germany), R&D Systems (Minneapolis, MN), Rockland Immunochemicals (Limerick, PA), and Thermo Fisher Scientific as follows: BD Phosflow Lyse/Fix buffer (catalog number 558049), BD Phosflow Perm Buffer III (catalog number 558050), BD Brilliant Stain Buffer (catalog number 566349), BD BSA stain buffer (catalog number 554657), Phycoerythrin-conjugated mouse antihuman CD42a antibody (catalog number 558819), FITC-conjugated mouse antihuman CD3 antibody (catalog number 561807), and Alexa Fluor 647econjugated mouse antihuman Stat5 pY694 antibody (catalog number 562076) from BD Biosciences; phycoerythrin-conjugated mouse anti-STAT4 (pY693) (catalog number 941204), Alexa Fluor 647econjugated mouse antihuman CD161 antibody (catalog number 339910), Alexa Fluor 647econjugated mouse antihuman CD61 antibody (catalog number 336408), Brilliant Violet 421econjugated mouse antihuman STAT3 Phospho (Tyr705) antibody (catalog number 651010), Brilliant Violet 605econjugated mouse antihuman CD62P antibody (catalog number 304920), Alexa Fluor 647econjugated mouse antihuman STAT4 Phospho (Tyr693) antibody (catalog number 941204), APCCy7econjugated mouse antihuman CD41 antibody (catalog number 303716), and Fc receptor blocking solution (catalog number 422302) from BioLegend; Fc receptor binding inhibitor polyclonal antibody (catalog number 14-9161-71) from eBioscience; AutoMACS running buffer (catalog number 130-091-221) from Miltenyi Biotec; reconstitution buffer (catalog number RB05 or RB02), Rockland Immunochemicals BSA 30% solution (lot number 49664), BD BSA stain buffer (catalog number BSA-30), and FACS buffer comprising PBS þ 1% BSA from R&D Systems; and Hanks’ Balanced Salt Solution (catalog number 14025092), FITC-conjugated mouse antihuman STAT3 Phospho (Tyr705) antibody (catalog number 11-9033-42), and recombinant human ILs from Thermo Fisher Scientific. .. Type 1 IFN and thrombopoietin were obtained as follows: recombinant human IL-2 (catalog number PHC00267, Life Technologies, Carlsbad, CA), recombinant human IL-12 (catalog number 200-12, PeproTech US, Cranbury, NJ), recombinant human IL-18 (catalog number 9124-IL-050, R&D Systems), HumanKine recombinant human IL-23 (catalog number HZ-1254, Proteintech, Rosemont, IL), recombinant human type 1 IFN (catalog number 11200-1, PBL Assay Science, Piscataway, NJ), and recombinant human thrombopoietin (catalog number 300-18, Thermo Fisher Scientific).
Staining:Article Title: Pharmacological Characterization of Zasocitinib (TAK-279): An Oral, Highly Selective, and Potent Allosteric TYK2 Inhibitor.
Article Snippet: .. Buffers and antibodies for flow cytometric analysis of signal transducer and activator of transcription (STAT) phosphorylation were obtained from BD Biosciences (San Diego, CA), BioLegend (San Diego, CA), and eBioscience (San Diego, CA), Miltenyi Biotec (Westphalia, Germany), R&D Systems (Minneapolis, MN), Rockland Immunochemicals (Limerick, PA), and Thermo Fisher Scientific as follows: BD Phosflow Lyse/Fix buffer (catalog number 558049), BD Phosflow Perm Buffer III (catalog number 558050), BD Brilliant Stain Buffer (catalog number 566349), BD BSA stain buffer (catalog number 554657), Phycoerythrin-conjugated mouse antihuman CD42a antibody (catalog number 558819), FITC-conjugated mouse antihuman CD3 antibody (catalog number 561807), and Alexa Fluor 647econjugated mouse antihuman Stat5 pY694 antibody (catalog number 562076) from BD Biosciences; phycoerythrin-conjugated mouse anti-STAT4 (pY693) (catalog number 941204), Alexa Fluor 647econjugated mouse antihuman CD161 antibody (catalog number 339910), Alexa Fluor 647econjugated mouse antihuman CD61 antibody (catalog number 336408), Brilliant Violet 421econjugated mouse antihuman STAT3 Phospho (Tyr705) antibody (catalog number 651010), Brilliant Violet 605econjugated mouse antihuman CD62P antibody (catalog number 304920), Alexa Fluor 647econjugated mouse antihuman STAT4 Phospho (Tyr693) antibody (catalog number 941204), APCCy7econjugated mouse antihuman CD41 antibody (catalog number 303716), and Fc receptor blocking solution (catalog number 422302) from BioLegend; Fc receptor binding inhibitor polyclonal antibody (catalog number 14-9161-71) from eBioscience; AutoMACS running buffer (catalog number 130-091-221) from Miltenyi Biotec; reconstitution buffer (catalog number RB05 or RB02), Rockland Immunochemicals BSA 30% solution (lot number 49664), BD BSA stain buffer (catalog number BSA-30), and FACS buffer comprising PBS þ 1% BSA from R&D Systems; and Hanks’ Balanced Salt Solution (catalog number 14025092), FITC-conjugated mouse antihuman STAT3 Phospho (Tyr705) antibody (catalog number 11-9033-42), and recombinant human ILs from Thermo Fisher Scientific. .. Type 1 IFN and thrombopoietin were obtained as follows: recombinant human IL-2 (catalog number PHC00267, Life Technologies, Carlsbad, CA), recombinant human IL-12 (catalog number 200-12, PeproTech US, Cranbury, NJ), recombinant human IL-18 (catalog number 9124-IL-050, R&D Systems), HumanKine recombinant human IL-23 (catalog number HZ-1254, Proteintech, Rosemont, IL), recombinant human type 1 IFN (catalog number 11200-1, PBL Assay Science, Piscataway, NJ), and recombinant human thrombopoietin (catalog number 300-18, Thermo Fisher Scientific).
Blocking Assay:Article Title: Pharmacological Characterization of Zasocitinib (TAK-279): An Oral, Highly Selective, and Potent Allosteric TYK2 Inhibitor.
Article Snippet: .. Buffers and antibodies for flow cytometric analysis of signal transducer and activator of transcription (STAT) phosphorylation were obtained from BD Biosciences (San Diego, CA), BioLegend (San Diego, CA), and eBioscience (San Diego, CA), Miltenyi Biotec (Westphalia, Germany), R&D Systems (Minneapolis, MN), Rockland Immunochemicals (Limerick, PA), and Thermo Fisher Scientific as follows: BD Phosflow Lyse/Fix buffer (catalog number 558049), BD Phosflow Perm Buffer III (catalog number 558050), BD Brilliant Stain Buffer (catalog number 566349), BD BSA stain buffer (catalog number 554657), Phycoerythrin-conjugated mouse antihuman CD42a antibody (catalog number 558819), FITC-conjugated mouse antihuman CD3 antibody (catalog number 561807), and Alexa Fluor 647econjugated mouse antihuman Stat5 pY694 antibody (catalog number 562076) from BD Biosciences; phycoerythrin-conjugated mouse anti-STAT4 (pY693) (catalog number 941204), Alexa Fluor 647econjugated mouse antihuman CD161 antibody (catalog number 339910), Alexa Fluor 647econjugated mouse antihuman CD61 antibody (catalog number 336408), Brilliant Violet 421econjugated mouse antihuman STAT3 Phospho (Tyr705) antibody (catalog number 651010), Brilliant Violet 605econjugated mouse antihuman CD62P antibody (catalog number 304920), Alexa Fluor 647econjugated mouse antihuman STAT4 Phospho (Tyr693) antibody (catalog number 941204), APCCy7econjugated mouse antihuman CD41 antibody (catalog number 303716), and Fc receptor blocking solution (catalog number 422302) from BioLegend; Fc receptor binding inhibitor polyclonal antibody (catalog number 14-9161-71) from eBioscience; AutoMACS running buffer (catalog number 130-091-221) from Miltenyi Biotec; reconstitution buffer (catalog number RB05 or RB02), Rockland Immunochemicals BSA 30% solution (lot number 49664), BD BSA stain buffer (catalog number BSA-30), and FACS buffer comprising PBS þ 1% BSA from R&D Systems; and Hanks’ Balanced Salt Solution (catalog number 14025092), FITC-conjugated mouse antihuman STAT3 Phospho (Tyr705) antibody (catalog number 11-9033-42), and recombinant human ILs from Thermo Fisher Scientific. .. Type 1 IFN and thrombopoietin were obtained as follows: recombinant human IL-2 (catalog number PHC00267, Life Technologies, Carlsbad, CA), recombinant human IL-12 (catalog number 200-12, PeproTech US, Cranbury, NJ), recombinant human IL-18 (catalog number 9124-IL-050, R&D Systems), HumanKine recombinant human IL-23 (catalog number HZ-1254, Proteintech, Rosemont, IL), recombinant human type 1 IFN (catalog number 11200-1, PBL Assay Science, Piscataway, NJ), and recombinant human thrombopoietin (catalog number 300-18, Thermo Fisher Scientific).
Binding Assay:Article Title: Pharmacological Characterization of Zasocitinib (TAK-279): An Oral, Highly Selective, and Potent Allosteric TYK2 Inhibitor.
Article Snippet: .. Buffers and antibodies for flow cytometric analysis of signal transducer and activator of transcription (STAT) phosphorylation were obtained from BD Biosciences (San Diego, CA), BioLegend (San Diego, CA), and eBioscience (San Diego, CA), Miltenyi Biotec (Westphalia, Germany), R&D Systems (Minneapolis, MN), Rockland Immunochemicals (Limerick, PA), and Thermo Fisher Scientific as follows: BD Phosflow Lyse/Fix buffer (catalog number 558049), BD Phosflow Perm Buffer III (catalog number 558050), BD Brilliant Stain Buffer (catalog number 566349), BD BSA stain buffer (catalog number 554657), Phycoerythrin-conjugated mouse antihuman CD42a antibody (catalog number 558819), FITC-conjugated mouse antihuman CD3 antibody (catalog number 561807), and Alexa Fluor 647econjugated mouse antihuman Stat5 pY694 antibody (catalog number 562076) from BD Biosciences; phycoerythrin-conjugated mouse anti-STAT4 (pY693) (catalog number 941204), Alexa Fluor 647econjugated mouse antihuman CD161 antibody (catalog number 339910), Alexa Fluor 647econjugated mouse antihuman CD61 antibody (catalog number 336408), Brilliant Violet 421econjugated mouse antihuman STAT3 Phospho (Tyr705) antibody (catalog number 651010), Brilliant Violet 605econjugated mouse antihuman CD62P antibody (catalog number 304920), Alexa Fluor 647econjugated mouse antihuman STAT4 Phospho (Tyr693) antibody (catalog number 941204), APCCy7econjugated mouse antihuman CD41 antibody (catalog number 303716), and Fc receptor blocking solution (catalog number 422302) from BioLegend; Fc receptor binding inhibitor polyclonal antibody (catalog number 14-9161-71) from eBioscience; AutoMACS running buffer (catalog number 130-091-221) from Miltenyi Biotec; reconstitution buffer (catalog number RB05 or RB02), Rockland Immunochemicals BSA 30% solution (lot number 49664), BD BSA stain buffer (catalog number BSA-30), and FACS buffer comprising PBS þ 1% BSA from R&D Systems; and Hanks’ Balanced Salt Solution (catalog number 14025092), FITC-conjugated mouse antihuman STAT3 Phospho (Tyr705) antibody (catalog number 11-9033-42), and recombinant human ILs from Thermo Fisher Scientific. .. Type 1 IFN and thrombopoietin were obtained as follows: recombinant human IL-2 (catalog number PHC00267, Life Technologies, Carlsbad, CA), recombinant human IL-12 (catalog number 200-12, PeproTech US, Cranbury, NJ), recombinant human IL-18 (catalog number 9124-IL-050, R&D Systems), HumanKine recombinant human IL-23 (catalog number HZ-1254, Proteintech, Rosemont, IL), recombinant human type 1 IFN (catalog number 11200-1, PBL Assay Science, Piscataway, NJ), and recombinant human thrombopoietin (catalog number 300-18, Thermo Fisher Scientific).
FACS:Article Title: Pharmacological Characterization of Zasocitinib (TAK-279): An Oral, Highly Selective, and Potent Allosteric TYK2 Inhibitor.
Article Snippet: .. Buffers and antibodies for flow cytometric analysis of signal transducer and activator of transcription (STAT) phosphorylation were obtained from BD Biosciences (San Diego, CA), BioLegend (San Diego, CA), and eBioscience (San Diego, CA), Miltenyi Biotec (Westphalia, Germany), R&D Systems (Minneapolis, MN), Rockland Immunochemicals (Limerick, PA), and Thermo Fisher Scientific as follows: BD Phosflow Lyse/Fix buffer (catalog number 558049), BD Phosflow Perm Buffer III (catalog number 558050), BD Brilliant Stain Buffer (catalog number 566349), BD BSA stain buffer (catalog number 554657), Phycoerythrin-conjugated mouse antihuman CD42a antibody (catalog number 558819), FITC-conjugated mouse antihuman CD3 antibody (catalog number 561807), and Alexa Fluor 647econjugated mouse antihuman Stat5 pY694 antibody (catalog number 562076) from BD Biosciences; phycoerythrin-conjugated mouse anti-STAT4 (pY693) (catalog number 941204), Alexa Fluor 647econjugated mouse antihuman CD161 antibody (catalog number 339910), Alexa Fluor 647econjugated mouse antihuman CD61 antibody (catalog number 336408), Brilliant Violet 421econjugated mouse antihuman STAT3 Phospho (Tyr705) antibody (catalog number 651010), Brilliant Violet 605econjugated mouse antihuman CD62P antibody (catalog number 304920), Alexa Fluor 647econjugated mouse antihuman STAT4 Phospho (Tyr693) antibody (catalog number 941204), APCCy7econjugated mouse antihuman CD41 antibody (catalog number 303716), and Fc receptor blocking solution (catalog number 422302) from BioLegend; Fc receptor binding inhibitor polyclonal antibody (catalog number 14-9161-71) from eBioscience; AutoMACS running buffer (catalog number 130-091-221) from Miltenyi Biotec; reconstitution buffer (catalog number RB05 or RB02), Rockland Immunochemicals BSA 30% solution (lot number 49664), BD BSA stain buffer (catalog number BSA-30), and FACS buffer comprising PBS þ 1% BSA from R&D Systems; and Hanks’ Balanced Salt Solution (catalog number 14025092), FITC-conjugated mouse antihuman STAT3 Phospho (Tyr705) antibody (catalog number 11-9033-42), and recombinant human ILs from Thermo Fisher Scientific. .. Type 1 IFN and thrombopoietin were obtained as follows: recombinant human IL-2 (catalog number PHC00267, Life Technologies, Carlsbad, CA), recombinant human IL-12 (catalog number 200-12, PeproTech US, Cranbury, NJ), recombinant human IL-18 (catalog number 9124-IL-050, R&D Systems), HumanKine recombinant human IL-23 (catalog number HZ-1254, Proteintech, Rosemont, IL), recombinant human type 1 IFN (catalog number 11200-1, PBL Assay Science, Piscataway, NJ), and recombinant human thrombopoietin (catalog number 300-18, Thermo Fisher Scientific).
other:Article Title: An efficient method to generate kidney organoids at the air-liquid interface.
Article Snippet: 9 Geltrex (Thermo fisher Scientific, Cat. # A1413301) 9 DMEM/F12 (Thermo fisher Scientific, Cat. # 11330-032) 9 StemFit (amsbio, Cat. # SFB-500) 9 FGF2 (R&D systems, Cat. # 234-FSE-025) 9 Rock inhibitor Y-27632 (EMD Millipore, Cat. # 688002-1mg) 9 H9 cell line (WiCell, Cat. # WA09) 9 Accutase (STEMCELL Technologies, Cat. # 7920) 9 Advanced RPMI 1640 (Thermo fisher Scientific, Cat. # 12633- 012) 9 Glutamax (Thermo fisher Scientific, Cat. # 35050061) 9 CHIR99021 IN SOLUTION (Reprocell, Cat. # 04-0004-10) 9 Activin A (R&D systems, Cat # 338-AC-010) 9 FGF9 (R&D systems, Cat. # 234-F9-025) 9 Heparin, (Sigma-Aldrich, Cat. # H3393-25KU) 9 TrypLe express ((Thermo fisher Scientific, Cat. # 12563029) 9 FBS (Atlanta Biologicals, Cat # S11550) 9 APEL2 (STEMCELL Technologies, Cat. # 5270) 9 PFHM II (Thermo fisher Scientific, Cat. # 12040077) 9 BMP7 (R&D systems, Cat. # 354-BP-010) 9 Reconstitution Buffer 4, BSA/HCl (R&D systems, Cat. # RB04) 9 Dextran, AF 488, 10000 MW (Thermo fisher Scientific, Cat. # D22910) 9 Paraformaldehyde (Sigma-Aldrich, Cat. # P6148-500G) 9 DPBS (Thermo fisher Scientific, Cat. # 14190144) 9 Triton X-100 (Sigma-Aldrich, Cat. # X100-100ML) 9 Normal Donkey Serum (Jackson ImmunoResearch, Cat. # 017-000-121) 9 Normal goat Serum (Jackson ImmunoResearch, Cat. # 005- 000-121) 9 BRN1 (Santa Cruz Biotechnology, Cat. # sc-6028-R) 9 CDH1 (Abcam, Cat. # ab11512) 9 CD31 (Cell Signaling Technology, Cat. # 3528) 9 DBA (Vector Laboratories, Cat. # B-1035) 9 GATA3 (Cell Signaling Technology, Cat. # 5852S) 9 HNF4A (Cell Signaling Technology, Cat. # 11F12) 9 LTL (Vector Laboratories, Cat. # B-1325) 9 Meis1 (Active Motif, Cat. # 39795) 9 PDGFRB (Abcam, Cat. # ab32570) 9 Podocalyxin (R&D systems, Cat. # AF1658) 9 DAPI (Thermo Fisher Scientific, Cat. # D1306) 9 Donkey anti-Goat IgG, Alexa Fluor 568 (Thermo Fisher Scientific, Cat. # A-11057) 9 Donkey anti-rat IgG, Alexa Fluor 488 (Thermo Fisher Scien- tific, Cat. # A-21208) 9 Streptavidin, Alexa Fluor (Thermo Fisher Scientific, Cat. # S21374) 9 Goat anti-Mouse IgG1, Alexa Fluor 568, (Thermo Fisher Scientific, Cat. # A-21124) 9 Donkey anti-Rabbit IgG, Alexa Fluor 488 (Thermo Fisher Scientific, Cat. # A-21206)
Control:
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