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bssh ii  (New England Biolabs)


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    Structured Review

    New England Biolabs bssh ii
    Bssh Ii, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 380 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsshii/BssHII/pmc13030959-35-0-3
    Average 96 stars, based on 380 article reviews
    bssh ii - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Recombinant:

    Article Title: MASTR-seq enables multiplexed analysis of short tandem repeats with sequencing.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples FXS human caudate nucleus brain tissue from NIH donor 5006 (Designated as FXS#5006) NIH NeuroBioBank https://neurobiobank.nih.gov FXS patient postmortem brain tissues #1031-08- GP were obtained UC-Davis (Designated as 103108GP) UC-Davis MIND Institute 1031-08-GP Chemicals, peptides, and recombinant proteins mTeSR Plus media STEMCELL Technology Cat# 100-0276 Matrigel hESC-Qualified Matrix Corning Cat# 354277 1X PBS without calcium and magnesium Corning Cat# 21-040-CV Penicillin-streptomycin Gibco Cat# 15140122 TrypLETM Express Enzyme (1X), phenol red Gibco Cat# 12605010 Versene Solution Gibco Cat# 15040066 10X TAE buffer Invitrogen Cat# 15558042 1M Tris-HCl (pH 8.0) Invitrogen Cat# 15568025 0.5M EDTA (pH 8.0) Invitrogen Cat# 15575020 20% SDS (w/v) solution Fisher Scientific Cat# BP1311-200 RNAse A Sigma-Aldrich Cat# 10109142001 Proteinase K New England Biolabs Cat# P8107S UltraPure Phenol/Chloroform/Isoamyl Alcohol Invitrogen Cat# 15593049 5M Ammonium Acetate Invitrogen Cat# AM9070G 100% Ethanol Decon Labs Cat# 2716 0.75% Agarose Gel Cassette Sage Science Cat# BLF7510 S. pyogenes Cas9 tracrRNA Integrated DNA Technologies Cat# 1072532 Alt-R S. pyogenes HiFi Cas9 nuclease V3 Integrated DNA Technologies Cat# 1081060 Quick Calf Intestinal Phosphatase New England Biolabs Cat# M0525S rCutSmart buffer New England Biolabs Cat# B6004S Nuclease-free water ThermoFisher Cat# AM9937 Taq DNA polymerase New England Biolabs Cat# M0273S 100 mM dATP solution Thermo Scientific, Cat# R0141 Nuclease-free duplex buffer Integrated DNA Technologies Cat# 11-01-03-01 Quick-Load 1 kb Extend DNA Ladder New England Biolabs Cat# N3239S NEB Blunt/TA Ligase Master Mix New England Biolabs Cat# M0367L T4 DNA ligase, from NEBNext Quick Ligation Module New England Biolabs Cat# E6056S EcoRI New England Biolabs Cat# R0101S BssH II New England Biolabs Cat# R0199S AP Detection buffer 10X (Alkaline Phos. buffer) Gene Link Cat# 40-5031-10 AP Substrate CDP Star Tropix Gene Link Cat# 40-5010-10 20X SSC Gene Link Cat# 40-5020-25 DIG Easy Hyb Roche Cat# T3010S Fragile X GeneProberTM GLFXDig1 Probe Digoxigenin labeled Gene Link Cat# 40-2004-41 Blocking solution 10% Gene Link Cat# 40-5026-10 Maleic acid buffer 10X (Buffer M 10X) Gene Link Cat# 40-5025-20 (Continued on next page) e1 Cell Reports Methods 6, 101341, March 23, 2026 Article ll OPEN ACCESS ..

    Agarose Gel Electrophoresis:

    Article Title: MASTR-seq enables multiplexed analysis of short tandem repeats with sequencing.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples FXS human caudate nucleus brain tissue from NIH donor 5006 (Designated as FXS#5006) NIH NeuroBioBank https://neurobiobank.nih.gov FXS patient postmortem brain tissues #1031-08- GP were obtained UC-Davis (Designated as 103108GP) UC-Davis MIND Institute 1031-08-GP Chemicals, peptides, and recombinant proteins mTeSR Plus media STEMCELL Technology Cat# 100-0276 Matrigel hESC-Qualified Matrix Corning Cat# 354277 1X PBS without calcium and magnesium Corning Cat# 21-040-CV Penicillin-streptomycin Gibco Cat# 15140122 TrypLETM Express Enzyme (1X), phenol red Gibco Cat# 12605010 Versene Solution Gibco Cat# 15040066 10X TAE buffer Invitrogen Cat# 15558042 1M Tris-HCl (pH 8.0) Invitrogen Cat# 15568025 0.5M EDTA (pH 8.0) Invitrogen Cat# 15575020 20% SDS (w/v) solution Fisher Scientific Cat# BP1311-200 RNAse A Sigma-Aldrich Cat# 10109142001 Proteinase K New England Biolabs Cat# P8107S UltraPure Phenol/Chloroform/Isoamyl Alcohol Invitrogen Cat# 15593049 5M Ammonium Acetate Invitrogen Cat# AM9070G 100% Ethanol Decon Labs Cat# 2716 0.75% Agarose Gel Cassette Sage Science Cat# BLF7510 S. pyogenes Cas9 tracrRNA Integrated DNA Technologies Cat# 1072532 Alt-R S. pyogenes HiFi Cas9 nuclease V3 Integrated DNA Technologies Cat# 1081060 Quick Calf Intestinal Phosphatase New England Biolabs Cat# M0525S rCutSmart buffer New England Biolabs Cat# B6004S Nuclease-free water ThermoFisher Cat# AM9937 Taq DNA polymerase New England Biolabs Cat# M0273S 100 mM dATP solution Thermo Scientific, Cat# R0141 Nuclease-free duplex buffer Integrated DNA Technologies Cat# 11-01-03-01 Quick-Load 1 kb Extend DNA Ladder New England Biolabs Cat# N3239S NEB Blunt/TA Ligase Master Mix New England Biolabs Cat# M0367L T4 DNA ligase, from NEBNext Quick Ligation Module New England Biolabs Cat# E6056S EcoRI New England Biolabs Cat# R0101S BssH II New England Biolabs Cat# R0199S AP Detection buffer 10X (Alkaline Phos. buffer) Gene Link Cat# 40-5031-10 AP Substrate CDP Star Tropix Gene Link Cat# 40-5010-10 20X SSC Gene Link Cat# 40-5020-25 DIG Easy Hyb Roche Cat# T3010S Fragile X GeneProberTM GLFXDig1 Probe Digoxigenin labeled Gene Link Cat# 40-2004-41 Blocking solution 10% Gene Link Cat# 40-5026-10 Maleic acid buffer 10X (Buffer M 10X) Gene Link Cat# 40-5025-20 (Continued on next page) e1 Cell Reports Methods 6, 101341, March 23, 2026 Article ll OPEN ACCESS ..

    Article Title: CBP-IDRs regulate acetylation and gene expression
    Article Snippet: .. CBP WT -GFP and synthesized sequences were digested with MluI-HF and BssHII (NEB) and purified by agarose gel extraction. .. The digested CBP WT -GFP vector was dephosphorylated using Quick Calf Intestinal Phosphatase (CIP, NEB) before assembly using T4 DNA ligase (NEB).

    Ligation:

    Article Title: MASTR-seq enables multiplexed analysis of short tandem repeats with sequencing.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples FXS human caudate nucleus brain tissue from NIH donor 5006 (Designated as FXS#5006) NIH NeuroBioBank https://neurobiobank.nih.gov FXS patient postmortem brain tissues #1031-08- GP were obtained UC-Davis (Designated as 103108GP) UC-Davis MIND Institute 1031-08-GP Chemicals, peptides, and recombinant proteins mTeSR Plus media STEMCELL Technology Cat# 100-0276 Matrigel hESC-Qualified Matrix Corning Cat# 354277 1X PBS without calcium and magnesium Corning Cat# 21-040-CV Penicillin-streptomycin Gibco Cat# 15140122 TrypLETM Express Enzyme (1X), phenol red Gibco Cat# 12605010 Versene Solution Gibco Cat# 15040066 10X TAE buffer Invitrogen Cat# 15558042 1M Tris-HCl (pH 8.0) Invitrogen Cat# 15568025 0.5M EDTA (pH 8.0) Invitrogen Cat# 15575020 20% SDS (w/v) solution Fisher Scientific Cat# BP1311-200 RNAse A Sigma-Aldrich Cat# 10109142001 Proteinase K New England Biolabs Cat# P8107S UltraPure Phenol/Chloroform/Isoamyl Alcohol Invitrogen Cat# 15593049 5M Ammonium Acetate Invitrogen Cat# AM9070G 100% Ethanol Decon Labs Cat# 2716 0.75% Agarose Gel Cassette Sage Science Cat# BLF7510 S. pyogenes Cas9 tracrRNA Integrated DNA Technologies Cat# 1072532 Alt-R S. pyogenes HiFi Cas9 nuclease V3 Integrated DNA Technologies Cat# 1081060 Quick Calf Intestinal Phosphatase New England Biolabs Cat# M0525S rCutSmart buffer New England Biolabs Cat# B6004S Nuclease-free water ThermoFisher Cat# AM9937 Taq DNA polymerase New England Biolabs Cat# M0273S 100 mM dATP solution Thermo Scientific, Cat# R0141 Nuclease-free duplex buffer Integrated DNA Technologies Cat# 11-01-03-01 Quick-Load 1 kb Extend DNA Ladder New England Biolabs Cat# N3239S NEB Blunt/TA Ligase Master Mix New England Biolabs Cat# M0367L T4 DNA ligase, from NEBNext Quick Ligation Module New England Biolabs Cat# E6056S EcoRI New England Biolabs Cat# R0101S BssH II New England Biolabs Cat# R0199S AP Detection buffer 10X (Alkaline Phos. buffer) Gene Link Cat# 40-5031-10 AP Substrate CDP Star Tropix Gene Link Cat# 40-5010-10 20X SSC Gene Link Cat# 40-5020-25 DIG Easy Hyb Roche Cat# T3010S Fragile X GeneProberTM GLFXDig1 Probe Digoxigenin labeled Gene Link Cat# 40-2004-41 Blocking solution 10% Gene Link Cat# 40-5026-10 Maleic acid buffer 10X (Buffer M 10X) Gene Link Cat# 40-5025-20 (Continued on next page) e1 Cell Reports Methods 6, 101341, March 23, 2026 Article ll OPEN ACCESS ..

    Labeling:

    Article Title: MASTR-seq enables multiplexed analysis of short tandem repeats with sequencing.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples FXS human caudate nucleus brain tissue from NIH donor 5006 (Designated as FXS#5006) NIH NeuroBioBank https://neurobiobank.nih.gov FXS patient postmortem brain tissues #1031-08- GP were obtained UC-Davis (Designated as 103108GP) UC-Davis MIND Institute 1031-08-GP Chemicals, peptides, and recombinant proteins mTeSR Plus media STEMCELL Technology Cat# 100-0276 Matrigel hESC-Qualified Matrix Corning Cat# 354277 1X PBS without calcium and magnesium Corning Cat# 21-040-CV Penicillin-streptomycin Gibco Cat# 15140122 TrypLETM Express Enzyme (1X), phenol red Gibco Cat# 12605010 Versene Solution Gibco Cat# 15040066 10X TAE buffer Invitrogen Cat# 15558042 1M Tris-HCl (pH 8.0) Invitrogen Cat# 15568025 0.5M EDTA (pH 8.0) Invitrogen Cat# 15575020 20% SDS (w/v) solution Fisher Scientific Cat# BP1311-200 RNAse A Sigma-Aldrich Cat# 10109142001 Proteinase K New England Biolabs Cat# P8107S UltraPure Phenol/Chloroform/Isoamyl Alcohol Invitrogen Cat# 15593049 5M Ammonium Acetate Invitrogen Cat# AM9070G 100% Ethanol Decon Labs Cat# 2716 0.75% Agarose Gel Cassette Sage Science Cat# BLF7510 S. pyogenes Cas9 tracrRNA Integrated DNA Technologies Cat# 1072532 Alt-R S. pyogenes HiFi Cas9 nuclease V3 Integrated DNA Technologies Cat# 1081060 Quick Calf Intestinal Phosphatase New England Biolabs Cat# M0525S rCutSmart buffer New England Biolabs Cat# B6004S Nuclease-free water ThermoFisher Cat# AM9937 Taq DNA polymerase New England Biolabs Cat# M0273S 100 mM dATP solution Thermo Scientific, Cat# R0141 Nuclease-free duplex buffer Integrated DNA Technologies Cat# 11-01-03-01 Quick-Load 1 kb Extend DNA Ladder New England Biolabs Cat# N3239S NEB Blunt/TA Ligase Master Mix New England Biolabs Cat# M0367L T4 DNA ligase, from NEBNext Quick Ligation Module New England Biolabs Cat# E6056S EcoRI New England Biolabs Cat# R0101S BssH II New England Biolabs Cat# R0199S AP Detection buffer 10X (Alkaline Phos. buffer) Gene Link Cat# 40-5031-10 AP Substrate CDP Star Tropix Gene Link Cat# 40-5010-10 20X SSC Gene Link Cat# 40-5020-25 DIG Easy Hyb Roche Cat# T3010S Fragile X GeneProberTM GLFXDig1 Probe Digoxigenin labeled Gene Link Cat# 40-2004-41 Blocking solution 10% Gene Link Cat# 40-5026-10 Maleic acid buffer 10X (Buffer M 10X) Gene Link Cat# 40-5025-20 (Continued on next page) e1 Cell Reports Methods 6, 101341, March 23, 2026 Article ll OPEN ACCESS ..

    Blocking Assay:

    Article Title: MASTR-seq enables multiplexed analysis of short tandem repeats with sequencing.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Biological samples FXS human caudate nucleus brain tissue from NIH donor 5006 (Designated as FXS#5006) NIH NeuroBioBank https://neurobiobank.nih.gov FXS patient postmortem brain tissues #1031-08- GP were obtained UC-Davis (Designated as 103108GP) UC-Davis MIND Institute 1031-08-GP Chemicals, peptides, and recombinant proteins mTeSR Plus media STEMCELL Technology Cat# 100-0276 Matrigel hESC-Qualified Matrix Corning Cat# 354277 1X PBS without calcium and magnesium Corning Cat# 21-040-CV Penicillin-streptomycin Gibco Cat# 15140122 TrypLETM Express Enzyme (1X), phenol red Gibco Cat# 12605010 Versene Solution Gibco Cat# 15040066 10X TAE buffer Invitrogen Cat# 15558042 1M Tris-HCl (pH 8.0) Invitrogen Cat# 15568025 0.5M EDTA (pH 8.0) Invitrogen Cat# 15575020 20% SDS (w/v) solution Fisher Scientific Cat# BP1311-200 RNAse A Sigma-Aldrich Cat# 10109142001 Proteinase K New England Biolabs Cat# P8107S UltraPure Phenol/Chloroform/Isoamyl Alcohol Invitrogen Cat# 15593049 5M Ammonium Acetate Invitrogen Cat# AM9070G 100% Ethanol Decon Labs Cat# 2716 0.75% Agarose Gel Cassette Sage Science Cat# BLF7510 S. pyogenes Cas9 tracrRNA Integrated DNA Technologies Cat# 1072532 Alt-R S. pyogenes HiFi Cas9 nuclease V3 Integrated DNA Technologies Cat# 1081060 Quick Calf Intestinal Phosphatase New England Biolabs Cat# M0525S rCutSmart buffer New England Biolabs Cat# B6004S Nuclease-free water ThermoFisher Cat# AM9937 Taq DNA polymerase New England Biolabs Cat# M0273S 100 mM dATP solution Thermo Scientific, Cat# R0141 Nuclease-free duplex buffer Integrated DNA Technologies Cat# 11-01-03-01 Quick-Load 1 kb Extend DNA Ladder New England Biolabs Cat# N3239S NEB Blunt/TA Ligase Master Mix New England Biolabs Cat# M0367L T4 DNA ligase, from NEBNext Quick Ligation Module New England Biolabs Cat# E6056S EcoRI New England Biolabs Cat# R0101S BssH II New England Biolabs Cat# R0199S AP Detection buffer 10X (Alkaline Phos. buffer) Gene Link Cat# 40-5031-10 AP Substrate CDP Star Tropix Gene Link Cat# 40-5010-10 20X SSC Gene Link Cat# 40-5020-25 DIG Easy Hyb Roche Cat# T3010S Fragile X GeneProberTM GLFXDig1 Probe Digoxigenin labeled Gene Link Cat# 40-2004-41 Blocking solution 10% Gene Link Cat# 40-5026-10 Maleic acid buffer 10X (Buffer M 10X) Gene Link Cat# 40-5025-20 (Continued on next page) e1 Cell Reports Methods 6, 101341, March 23, 2026 Article ll OPEN ACCESS ..

    Synthesized:

    Article Title: CBP-IDRs regulate acetylation and gene expression
    Article Snippet: .. CBP WT -GFP and synthesized sequences were digested with MluI-HF and BssHII (NEB) and purified by agarose gel extraction. .. The digested CBP WT -GFP vector was dephosphorylated using Quick Calf Intestinal Phosphatase (CIP, NEB) before assembly using T4 DNA ligase (NEB).

    Purification:

    Article Title: CBP-IDRs regulate acetylation and gene expression
    Article Snippet: .. CBP WT -GFP and synthesized sequences were digested with MluI-HF and BssHII (NEB) and purified by agarose gel extraction. .. The digested CBP WT -GFP vector was dephosphorylated using Quick Calf Intestinal Phosphatase (CIP, NEB) before assembly using T4 DNA ligase (NEB).

    Extraction:

    Article Title: CBP-IDRs regulate acetylation and gene expression
    Article Snippet: .. CBP WT -GFP and synthesized sequences were digested with MluI-HF and BssHII (NEB) and purified by agarose gel extraction. .. The digested CBP WT -GFP vector was dephosphorylated using Quick Calf Intestinal Phosphatase (CIP, NEB) before assembly using T4 DNA ligase (NEB).

    Polymerase Chain Reaction:

    Article Title: Duplication within 14q32.13 implicates a chimeric CLMN :: SYNE3 RNA transcript in cerebellar ataxia
    Article Snippet: .. The CLMN :: SYNE3 chimeric transcript PCR product digested with Xho I (NEB: R0146) and Bss HII (NEB: R0199) was cloned into the corresponding sites in the mEmerald-SYNE3 vector. .. To create the mEmerald-Empty-Vector, the mEmerald-Nesprin3-C-18 was digested with Xho I (NEB: R0146) and Kpn I-HF (NEB: R3142S), blunt ended using T4 DNA Polymerase (NEB: M020) and then re-ligated with the T4 DNA ligase (NEB: M0202), following manufacturer’s instructions.

    Clone Assay:

    Article Title: Duplication within 14q32.13 implicates a chimeric CLMN :: SYNE3 RNA transcript in cerebellar ataxia
    Article Snippet: .. The CLMN :: SYNE3 chimeric transcript PCR product digested with Xho I (NEB: R0146) and Bss HII (NEB: R0199) was cloned into the corresponding sites in the mEmerald-SYNE3 vector. .. To create the mEmerald-Empty-Vector, the mEmerald-Nesprin3-C-18 was digested with Xho I (NEB: R0146) and Kpn I-HF (NEB: R3142S), blunt ended using T4 DNA Polymerase (NEB: M020) and then re-ligated with the T4 DNA ligase (NEB: M0202), following manufacturer’s instructions.

    other:

    Article Title: CBP-IDRs regulate acetylation and gene expression
    Article Snippet: BssHII , NEB , R0199S.

    Plasmid Preparation:

    Article Title: OT-Curtains: an approach for studying protein interactions with DNA ends using optical tweezers and confocal fluorescence microscopy
    Article Snippet: .. The 22 142 bp 4x poly-BbvCI plasmid (see previous section) at a final concentration of 2.7 nM is digested with 1.25 units of BssHII (New England Biolabs, #R0199S) to linearize the DNA in 6.25 μl buffer provided by the manufacturer. .. Simultaneously, the plasmid is treated with 6 units of the nicking enzyme Nt.BbvCI (New England Biolabs, #R0362S).

    Concentration Assay:

    Article Title: OT-Curtains: an approach for studying protein interactions with DNA ends using optical tweezers and confocal fluorescence microscopy
    Article Snippet: .. The 22 142 bp 4x poly-BbvCI plasmid (see previous section) at a final concentration of 2.7 nM is digested with 1.25 units of BssHII (New England Biolabs, #R0199S) to linearize the DNA in 6.25 μl buffer provided by the manufacturer. .. Simultaneously, the plasmid is treated with 6 units of the nicking enzyme Nt.BbvCI (New England Biolabs, #R0362S).



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