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r0199s  (New England Biolabs)


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    Structured Review

    New England Biolabs r0199s
    R0199s, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 96/100, based on 382 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsshii/BssHII/pmc13013760-51-4-2
    Average 96 stars, based on 382 article reviews
    r0199s - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Mutagenesis:

    Article Title: Live imaging and interactome analysis of Zika and chikungunya viral RNAs via dual-action aptamer tag
    Article Snippet: The pLenti-mCherry-Mango-II x 24 plasmid was obtained from Addgene (#127587), andMango-II triplet inserts were amplified by PCR (Supplementary data 1) using Q5 High-Fidelity 2X Master mix (New England Biolabs, M0492S) with primers containing BssHII or BamHI restriction enzyme digestion sites (Supplementary data 1) and purified using a 10% acrylamide gel. .. A BssHII site was introduced into ZIKV cDNA between NS1-NS2A and a BamHI site was introduced into 181/25 cDNA between nsP3385-386 (Supplementary data 1) by site-directed mutagenesis using Phusion DNA polymerase (New England Biolabs, #MO530), and Mango-II triplet was cloned into the cDNA clone by restriction digestion followed by ligation using T4 DNA ligase (New England Biolabs, 0202S). .. The cDNA plasmid of CHIKV-FP was generated by removing the Mango-II triplet from CHIKV-MFP via sitedirected mutagenesis using Phusion DNA polymerase (New England Biolabs, #MO530).

    Clone Assay:

    Article Title: Live imaging and interactome analysis of Zika and chikungunya viral RNAs via dual-action aptamer tag
    Article Snippet: The pLenti-mCherry-Mango-II x 24 plasmid was obtained from Addgene (#127587), andMango-II triplet inserts were amplified by PCR (Supplementary data 1) using Q5 High-Fidelity 2X Master mix (New England Biolabs, M0492S) with primers containing BssHII or BamHI restriction enzyme digestion sites (Supplementary data 1) and purified using a 10% acrylamide gel. .. A BssHII site was introduced into ZIKV cDNA between NS1-NS2A and a BamHI site was introduced into 181/25 cDNA between nsP3385-386 (Supplementary data 1) by site-directed mutagenesis using Phusion DNA polymerase (New England Biolabs, #MO530), and Mango-II triplet was cloned into the cDNA clone by restriction digestion followed by ligation using T4 DNA ligase (New England Biolabs, 0202S). .. The cDNA plasmid of CHIKV-FP was generated by removing the Mango-II triplet from CHIKV-MFP via sitedirected mutagenesis using Phusion DNA polymerase (New England Biolabs, #MO530).

    Article Title: Duplication within 14q32.13 implicates a chimeric CLMN :: SYNE3 RNA transcript in cerebellar ataxia
    Article Snippet: .. The CLMN :: SYNE3 chimeric transcript PCR product digested with Xho I (NEB: R0146) and Bss HII (NEB: R0199) was cloned into the corresponding sites in the mEmerald-SYNE3 vector. .. To create the mEmerald-Empty-Vector, the mEmerald-Nesprin3-C-18 was digested with Xho I (NEB: R0146) and Kpn I-HF (NEB: R3142S), blunt ended using T4 DNA Polymerase (NEB: M020) and then re-ligated with the T4 DNA ligase (NEB: M0202), following manufacturer’s instructions.

    Restriction Assay:

    Article Title: Live imaging and interactome analysis of Zika and chikungunya viral RNAs via dual-action aptamer tag
    Article Snippet: The pLenti-mCherry-Mango-II x 24 plasmid was obtained from Addgene (#127587), andMango-II triplet inserts were amplified by PCR (Supplementary data 1) using Q5 High-Fidelity 2X Master mix (New England Biolabs, M0492S) with primers containing BssHII or BamHI restriction enzyme digestion sites (Supplementary data 1) and purified using a 10% acrylamide gel. .. A BssHII site was introduced into ZIKV cDNA between NS1-NS2A and a BamHI site was introduced into 181/25 cDNA between nsP3385-386 (Supplementary data 1) by site-directed mutagenesis using Phusion DNA polymerase (New England Biolabs, #MO530), and Mango-II triplet was cloned into the cDNA clone by restriction digestion followed by ligation using T4 DNA ligase (New England Biolabs, 0202S). .. The cDNA plasmid of CHIKV-FP was generated by removing the Mango-II triplet from CHIKV-MFP via sitedirected mutagenesis using Phusion DNA polymerase (New England Biolabs, #MO530).

    Ligation:

    Article Title: Live imaging and interactome analysis of Zika and chikungunya viral RNAs via dual-action aptamer tag
    Article Snippet: The pLenti-mCherry-Mango-II x 24 plasmid was obtained from Addgene (#127587), andMango-II triplet inserts were amplified by PCR (Supplementary data 1) using Q5 High-Fidelity 2X Master mix (New England Biolabs, M0492S) with primers containing BssHII or BamHI restriction enzyme digestion sites (Supplementary data 1) and purified using a 10% acrylamide gel. .. A BssHII site was introduced into ZIKV cDNA between NS1-NS2A and a BamHI site was introduced into 181/25 cDNA between nsP3385-386 (Supplementary data 1) by site-directed mutagenesis using Phusion DNA polymerase (New England Biolabs, #MO530), and Mango-II triplet was cloned into the cDNA clone by restriction digestion followed by ligation using T4 DNA ligase (New England Biolabs, 0202S). .. The cDNA plasmid of CHIKV-FP was generated by removing the Mango-II triplet from CHIKV-MFP via sitedirected mutagenesis using Phusion DNA polymerase (New England Biolabs, #MO530).

    other:

    Article Title: CBP-IDRs regulate acetylation and gene expression
    Article Snippet: Fully assembled CBP WT -GFP was sequenced by Sanger sequencing using sequential primers at 900bp intervals and confirmed using Whole Plasmid Sequencing (Eurofins) to check for correct assembly.

    Article Title: MASTR-seq enables multiplexed analysis of short tandem repeats with sequencing
    Article Snippet: BssH II , New England Biolabs , Cat# R0199S.

    Article Title: CBP-IDRs regulate acetylation and gene expression
    Article Snippet: BssHII , NEB , R0199S.

    Polymerase Chain Reaction:

    Article Title: Duplication within 14q32.13 implicates a chimeric CLMN :: SYNE3 RNA transcript in cerebellar ataxia
    Article Snippet: .. The CLMN :: SYNE3 chimeric transcript PCR product digested with Xho I (NEB: R0146) and Bss HII (NEB: R0199) was cloned into the corresponding sites in the mEmerald-SYNE3 vector. .. To create the mEmerald-Empty-Vector, the mEmerald-Nesprin3-C-18 was digested with Xho I (NEB: R0146) and Kpn I-HF (NEB: R3142S), blunt ended using T4 DNA Polymerase (NEB: M020) and then re-ligated with the T4 DNA ligase (NEB: M0202), following manufacturer’s instructions.

    Plasmid Preparation:

    Article Title: OT-Curtains: an approach for studying protein interactions with DNA ends using optical tweezers and confocal fluorescence microscopy
    Article Snippet: .. The 22 142 bp 4x poly-BbvCI plasmid (see previous section) at a final concentration of 2.7 nM is digested with 1.25 units of BssHII (New England Biolabs, #R0199S) to linearize the DNA in 6.25 μl buffer provided by the manufacturer. .. Simultaneously, the plasmid is treated with 6 units of the nicking enzyme Nt.BbvCI (New England Biolabs, #R0362S).

    Concentration Assay:

    Article Title: OT-Curtains: an approach for studying protein interactions with DNA ends using optical tweezers and confocal fluorescence microscopy
    Article Snippet: .. The 22 142 bp 4x poly-BbvCI plasmid (see previous section) at a final concentration of 2.7 nM is digested with 1.25 units of BssHII (New England Biolabs, #R0199S) to linearize the DNA in 6.25 μl buffer provided by the manufacturer. .. Simultaneously, the plasmid is treated with 6 units of the nicking enzyme Nt.BbvCI (New England Biolabs, #R0362S).



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