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Structured Review

Promega 4.0-kb bamhi/bsshii fragment
4.0 Kb Bamhi/Bsshii Fragment, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bsshii/4+0+kb+bamhi+bsshii+fragment/pm09030575-60-33-18
Average 90 stars, based on 1 article reviews
4.0-kb bamhi/bsshii fragment - by Bioz Stars, 2026-10
90/100 stars

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Related Articles

Plasmid Preparation:

Article Title: Isolation and characterization of the 5'-upstream region of the human N-type calcium channel alpha1B subunit gene. Chromosomal localization and promoter analysis.
Article Snippet: Plasmid Construction—The human a1B gene-luciferase fusion plasmids were constructed by subcloning into the polylinker region of pGL2Basic vector (Promega) with the following restriction fragments from the human a1B genomic clone pNAG Sac2-2: a 4.0-kb BamHI/BssHII fragment (23992L), a 1.7-kb XhoI/BssHII fragment (21788L), and 0.1-kb NotI/BssHII fragment (2110L).

Construct:

Article Title: Isolation and characterization of the 5'-upstream region of the human N-type calcium channel alpha1B subunit gene. Chromosomal localization and promoter analysis.
Article Snippet: Plasmid Construction—The human a1B gene-luciferase fusion plasmids were constructed by subcloning into the polylinker region of pGL2Basic vector (Promega) with the following restriction fragments from the human a1B genomic clone pNAG Sac2-2: a 4.0-kb BamHI/BssHII fragment (23992L), a 1.7-kb XhoI/BssHII fragment (21788L), and 0.1-kb NotI/BssHII fragment (2110L).

Subcloning:

Article Title: Isolation and characterization of the 5'-upstream region of the human N-type calcium channel alpha1B subunit gene. Chromosomal localization and promoter analysis.
Article Snippet: Plasmid Construction—The human a1B gene-luciferase fusion plasmids were constructed by subcloning into the polylinker region of pGL2Basic vector (Promega) with the following restriction fragments from the human a1B genomic clone pNAG Sac2-2: a 4.0-kb BamHI/BssHII fragment (23992L), a 1.7-kb XhoI/BssHII fragment (21788L), and 0.1-kb NotI/BssHII fragment (2110L).



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