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Applygen Technologies w v bovine serum albumin bsa p1621 applygen china
W V Bovine Serum Albumin Bsa P1621 Applygen China, supplied by Applygen Technologies, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bsa/albumin+bovine+serum/pmc12972744-667-6-12
Average 86 stars, based on 1 article reviews
w v bovine serum albumin bsa p1621 applygen china - by Bioz Stars, 2026-10
86/100 stars

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Article Title: Membralin Assembles a MAN1B1–VCP Complex to Target Foreign Glycoproteins from the Endoplasmic Reticulum to Lysosomes for Degradation
Article Snippet: [ ] After 30 h, cells were fixed with 4% paraformaldehyde for 10 min, permeabilized with 0.1% Triton X‐100 (Solarbio Life Sciences, T8200) for 10 min at room temperature, and then blocked with 5% bovine serum albumin (BSA; APPLYGEN, P1622) solution overnight at 4 °C.

Article Title: Artificial mitochondria ameliorates osteoarthritis through restoring cellular energy metabolism homeostasis
Article Snippet: The membranes were blocked with 5% (w/v) bovine serum albumin (BSA) (P1621, Applygen, China) for 1 h at room temperature.

Article Title: Propranolol enhances the oncolytic effect of newcastle disease virus on canine mammary tumor cell by modulating the IFN-I-mediated JAK-STAT signaling pathway.
Article Snippet: At 24 h.p.i, cells were fixed with 4% paraformaldehyde (105008, Xilong Scientific, Shantou, China), permeabilized with 0.1% Triton X-100 (ST797, Beyotime, Shanghai, China) in PBST, and blocked with 5% bovine serum albumin (BSA, Applygen Technologies Inc., P1621, Beijing, China) solution.

Article Title: SENP3 Promotes Hepatocyte Steatosis via De-SUMOylation of SREBP2.
Article Snippet: 20% Bovine serum albumin without free fatty acid Solution (A2000‐2) was purchased from Applygen (Beijing, China), and OA (O108487) was from Aladdin (Shanghai, China).

Article Title: FAM134B isoform 2/RETREG1-2 defines a calnexin–TOLLIP–coupled ER-phagy pathway that restricts Ebola virus glycoprotein and is antagonized by VP40 through macro-autophagy
Article Snippet: After 30 h, cells were fixed with 4% paraformaldehyde for 10 min, permeabilized with 0.1% Triton X-100 (Solarbio Life Sciences, T8200) for 10 min at room temperature, and then blocked with 5% bovine serum albumin (BSA; APPLYGEN, P1622) solution overnight at 4°C.

Article Title: Propranolol enhances the oncolytic effect of newcastle disease virus on canine mammary tumor cell by modulating the IFN-I-mediated JAK-STAT signaling pathway
Article Snippet: Treatments were administered as described in At 24 h.p.i, cells were fixed with 4% paraformaldehyde (105008, Xilong Scientific, Shantou, China), permeabilized with 0.1% Triton X-100 (ST797, Beyotime, Shanghai, China) in PBST, and blocked with 5% bovine serum albumin (BSA, Applygen Technologies Inc., P1621, Beijing, China) solution.

BIA-KA:

Article Title: Early postnatal antibiotic-associated gut microbiota alterations might promote long-term lipid metabolism via brown adipose tissue metabolic programming.
Article Snippet: .. Total protein concentrations were determined by the BCA assay using bovine serum albumin standards (APPLYGEN, Beijing, China) and normalized to equal concentrations. .. TG and TC levels in the supernatants were then quantified using commercial assay kits (APPLYGEN) by a Chemray 800 automated biochemistry analyzer.



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( A ) Representative parasternal long-axis views and M-mode images. ( B ) Echocardiographic analysis of EF, FS, LVESV, LVEDV, LVESD, and LVEDD on days 0, 7, and 14 after MI or sham operation in Sik2 F/F and Sik2 F/F ; LysM Cre mice ( n = 8). ( C ) Masson’s trichrome staining for infarct size measurements in Sik2 F/F and Sik2 F/F ; LysM Cre mice at day 14 after MI or sham operation ( n = 8). ( D ) Post-MI survival analysis. n = 42 in Sik2 F/F MI; n = 38 in Sik2 F/F ; LysM Cre MI. ( E ) Masson’s trichrome and Picrosirius red (PSR) staining of heart transverse sections to detect collagen within the scar of Sik2 F/F and Sik2 F/F ; LysM Cre hearts at day 14 post-MI (scale bar, 100 μm). Collagen density measured as the total collagen area divided by scar area examined ( n = 7). ( F ) Immunostaining analyses of α-SMA (green), <t>Collagen</t> <t>I</t> (green) and DAPI (blue) in the heart tissue at day 7 post-MI ( n = 8) (scale bar, 40 μm). ROI indicates the region of interest. ( G ) Gating strategy for endothelial cells (ECs) and CFs in the infarcted heart. ( H ) Statistics of ECs and CFs in the infarcted heart at day 7 post-MI from Sik2 F/F and Sik2 F/F ; LysM Cre mice ( n = 6). ( I ) Representative fibroblasts immunostaining of α-SMA (green), Collagen I (red), and DAPI (blue). Scale bar, 20 μm.
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Antibodies.

Journal:

Article Title:

doi:

Figure Lengend Snippet: Antibodies.

Article Snippet: Western blot (mouse sample) , Novus Biologicals , NB100-2220.

Techniques: Western Blot

( A ) Representative parasternal long-axis views and M-mode images. ( B ) Echocardiographic analysis of EF, FS, LVESV, LVEDV, LVESD, and LVEDD on days 0, 7, and 14 after MI or sham operation in Sik2 F/F and Sik2 F/F ; LysM Cre mice ( n = 8). ( C ) Masson’s trichrome staining for infarct size measurements in Sik2 F/F and Sik2 F/F ; LysM Cre mice at day 14 after MI or sham operation ( n = 8). ( D ) Post-MI survival analysis. n = 42 in Sik2 F/F MI; n = 38 in Sik2 F/F ; LysM Cre MI. ( E ) Masson’s trichrome and Picrosirius red (PSR) staining of heart transverse sections to detect collagen within the scar of Sik2 F/F and Sik2 F/F ; LysM Cre hearts at day 14 post-MI (scale bar, 100 μm). Collagen density measured as the total collagen area divided by scar area examined ( n = 7). ( F ) Immunostaining analyses of α-SMA (green), Collagen I (green) and DAPI (blue) in the heart tissue at day 7 post-MI ( n = 8) (scale bar, 40 μm). ROI indicates the region of interest. ( G ) Gating strategy for endothelial cells (ECs) and CFs in the infarcted heart. ( H ) Statistics of ECs and CFs in the infarcted heart at day 7 post-MI from Sik2 F/F and Sik2 F/F ; LysM Cre mice ( n = 6). ( I ) Representative fibroblasts immunostaining of α-SMA (green), Collagen I (red), and DAPI (blue). Scale bar, 20 μm.

Journal: Science Advances

Article Title: SIK2 antagonizes postinfarction cardiac repair by driving macrophage proinflammatory and impairing efferocytosis

doi: 10.1126/sciadv.adw6624

Figure Lengend Snippet: ( A ) Representative parasternal long-axis views and M-mode images. ( B ) Echocardiographic analysis of EF, FS, LVESV, LVEDV, LVESD, and LVEDD on days 0, 7, and 14 after MI or sham operation in Sik2 F/F and Sik2 F/F ; LysM Cre mice ( n = 8). ( C ) Masson’s trichrome staining for infarct size measurements in Sik2 F/F and Sik2 F/F ; LysM Cre mice at day 14 after MI or sham operation ( n = 8). ( D ) Post-MI survival analysis. n = 42 in Sik2 F/F MI; n = 38 in Sik2 F/F ; LysM Cre MI. ( E ) Masson’s trichrome and Picrosirius red (PSR) staining of heart transverse sections to detect collagen within the scar of Sik2 F/F and Sik2 F/F ; LysM Cre hearts at day 14 post-MI (scale bar, 100 μm). Collagen density measured as the total collagen area divided by scar area examined ( n = 7). ( F ) Immunostaining analyses of α-SMA (green), Collagen I (green) and DAPI (blue) in the heart tissue at day 7 post-MI ( n = 8) (scale bar, 40 μm). ROI indicates the region of interest. ( G ) Gating strategy for endothelial cells (ECs) and CFs in the infarcted heart. ( H ) Statistics of ECs and CFs in the infarcted heart at day 7 post-MI from Sik2 F/F and Sik2 F/F ; LysM Cre mice ( n = 6). ( I ) Representative fibroblasts immunostaining of α-SMA (green), Collagen I (red), and DAPI (blue). Scale bar, 20 μm.

Article Snippet: Primary antibodies against the following targets were used in these experiments: SIK2 (6919s, 1:200; CST, Danvers, MA, USA), Ly6G (551459, 1:200; BD Biosciences, Franklin Lakes, NJ, USA), CD68 (ab53444, 1:200; Abcam, Cambridge, UK), TNNI3 (SAB2502170, 1:200; Sigma-Aldrich, St. Louis, MO, USA), α-SMA (A5228, 1:700; Sigma-Aldrich), Collagen I (NBP1-30054, 1:200; Novus Biologicals, Littleton, CO, USA), CD31 (ab222783, 1:200; Abcam), NOS2 (13120s, 1:200; CST), and Arginase-1 (93668s, 1:200; CST).

Techniques: Analysis, Staining, Immunostaining, Tissue

( A ) Representative TUNEL/TNNI3/DAPI staining of cardiomyocytes from Sik2 F/F and Sik2 F/F ; LysM Cre mice on day 3 post-MI. White arrows indicate TUNEL + (green) cardiomyocytes (red) and DAPI (blue) (scale bar, 40 μm). Percentage of TUNEL + cardiomyocytes ( n = 6). ( B ) Immunostaining shows colocalization of CD68 + macrophages with TNNI3 + cardiomyocytes (yellow). Scale bar, 40 μm. Macrophages positive for TNNI3 are scored as internalizing cardiomyocyte-derived proteins ( n = 6). ( C ) Heatmap of efferocytosis-related DEGs in cardiac macrophages on day 3 post-MI. ( D ) WT or SIK2-knockout cardiac macrophages were incubated with Calcein-AM + apoptotic cardiomyocytes (AC:Mφ = 2:1 or 5:1) for 1 hour. Efferocytosis was quantified as the total percentage of macrophages positive for Calcein-AM + ACs ( n = 4 for 2:1; n = 6 for 5:1). ( E ) Calcein-AM + ACs were incubated with WT or SIK2-knockout macrophages (5:1) for 1 hour. Efferocytosis was analyzed by fluorescence microscopy for Calcein-AM mean fluorescence intensity (MFI) in macrophage ( n = 7). ( F ) Flow cytometry analysis of efferocytosis in PMs for 2 hours. ( G ) PMs from WT and SIK2-cKO mice were treated with CFSE + RBCs (RBC:Mφ = 50:1 or 100:1) for 2 hours. Phagocytic macrophages were quantified as the total percentage of macrophages positive for CFSE + RBCs ( n = 5). ( H and I ) qPCR analysis of Cd36 , Cx3cr1 , Il-10 , Tgfb1 , and Vegfa mRNA levels in macrophages cocultured with RBCs for 8 hours ( n = 4). ( J ) qPCR analysis of α-SMA and Col1a1 mRNA levels in control and ARN3236 (3 μmol)–treated CFs with or without Tgfb1 (20 ng/ml) ( n = 6). ( K ) Immunostaining staining of α-SMA (green), Collagen I (red), and DAPI (blue) in WT mouse CFs treated with macrophage-conditioned media (ARN3236-treated or control) after efferocytosis. Scale bar, 20 μm. ( L ) qPCR analysis of α-SMA and Col1a1 mRNA levels in WT mouse CFs cultured with macrophage-conditioned media (ARN3236-treated or control) after efferocytosis ( n = 6).

Journal: Science Advances

Article Title: SIK2 antagonizes postinfarction cardiac repair by driving macrophage proinflammatory and impairing efferocytosis

doi: 10.1126/sciadv.adw6624

Figure Lengend Snippet: ( A ) Representative TUNEL/TNNI3/DAPI staining of cardiomyocytes from Sik2 F/F and Sik2 F/F ; LysM Cre mice on day 3 post-MI. White arrows indicate TUNEL + (green) cardiomyocytes (red) and DAPI (blue) (scale bar, 40 μm). Percentage of TUNEL + cardiomyocytes ( n = 6). ( B ) Immunostaining shows colocalization of CD68 + macrophages with TNNI3 + cardiomyocytes (yellow). Scale bar, 40 μm. Macrophages positive for TNNI3 are scored as internalizing cardiomyocyte-derived proteins ( n = 6). ( C ) Heatmap of efferocytosis-related DEGs in cardiac macrophages on day 3 post-MI. ( D ) WT or SIK2-knockout cardiac macrophages were incubated with Calcein-AM + apoptotic cardiomyocytes (AC:Mφ = 2:1 or 5:1) for 1 hour. Efferocytosis was quantified as the total percentage of macrophages positive for Calcein-AM + ACs ( n = 4 for 2:1; n = 6 for 5:1). ( E ) Calcein-AM + ACs were incubated with WT or SIK2-knockout macrophages (5:1) for 1 hour. Efferocytosis was analyzed by fluorescence microscopy for Calcein-AM mean fluorescence intensity (MFI) in macrophage ( n = 7). ( F ) Flow cytometry analysis of efferocytosis in PMs for 2 hours. ( G ) PMs from WT and SIK2-cKO mice were treated with CFSE + RBCs (RBC:Mφ = 50:1 or 100:1) for 2 hours. Phagocytic macrophages were quantified as the total percentage of macrophages positive for CFSE + RBCs ( n = 5). ( H and I ) qPCR analysis of Cd36 , Cx3cr1 , Il-10 , Tgfb1 , and Vegfa mRNA levels in macrophages cocultured with RBCs for 8 hours ( n = 4). ( J ) qPCR analysis of α-SMA and Col1a1 mRNA levels in control and ARN3236 (3 μmol)–treated CFs with or without Tgfb1 (20 ng/ml) ( n = 6). ( K ) Immunostaining staining of α-SMA (green), Collagen I (red), and DAPI (blue) in WT mouse CFs treated with macrophage-conditioned media (ARN3236-treated or control) after efferocytosis. Scale bar, 20 μm. ( L ) qPCR analysis of α-SMA and Col1a1 mRNA levels in WT mouse CFs cultured with macrophage-conditioned media (ARN3236-treated or control) after efferocytosis ( n = 6).

Article Snippet: Primary antibodies against the following targets were used in these experiments: SIK2 (6919s, 1:200; CST, Danvers, MA, USA), Ly6G (551459, 1:200; BD Biosciences, Franklin Lakes, NJ, USA), CD68 (ab53444, 1:200; Abcam, Cambridge, UK), TNNI3 (SAB2502170, 1:200; Sigma-Aldrich, St. Louis, MO, USA), α-SMA (A5228, 1:700; Sigma-Aldrich), Collagen I (NBP1-30054, 1:200; Novus Biologicals, Littleton, CO, USA), CD31 (ab222783, 1:200; Abcam), NOS2 (13120s, 1:200; CST), and Arginase-1 (93668s, 1:200; CST).

Techniques: TUNEL Assay, Staining, Immunostaining, Derivative Assay, Knock-Out, Incubation, Fluorescence, Microscopy, Flow Cytometry, Analysis, Control, Cell Culture