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wash buffer  (Novus Biologicals)


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    Novus Biologicals wash buffer
    Wash Buffer, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsa+buffer/c-Myc+Antibody+(Myc%2EA7)+-+Azide+and+BSA+Free/us12612461-525-6-16
    Average 93 stars, based on 10 article reviews
    wash buffer - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Single domain antibodies against CLL-1
    Article Snippet: .. Briefly, VHH clones (3 μg/ml as final conc./test) in FACS buffer were added to Fc blocked U937 cells (2.5×10{circumflex over ( )}5), the mixtures were incubated for 30 min at 4° C., then washed twice with FACS buffer by spinning at 500G for 2 min; anti-cMyc (Novus, Cat. #NBP2-37822) was added to cells, the mixture was incubated for 30 min/4° C. and washed twice with FACS buffer by spinning at 500G/2 min, then anti-msIgG-PE (Jackson ImmunoResearch, Cat. #115-116-146) was added to the cells, the mixture was incubated for 30 min/4° C. and washed twice with FACS buffer by spinning at 500G/2 min, 1% PFA (Paraformaldehyde) in PBS was added to cell pellet to fix the cells, followed by FACS analysis. ..

    FACS:

    Article Title: Single domain antibodies against CLL-1
    Article Snippet: .. Briefly, VHH clones (3 μg/ml as final conc./test) in FACS buffer were added to Fc blocked U937 cells (2.5×10{circumflex over ( )}5), the mixtures were incubated for 30 min at 4° C., then washed twice with FACS buffer by spinning at 500G for 2 min; anti-cMyc (Novus, Cat. #NBP2-37822) was added to cells, the mixture was incubated for 30 min/4° C. and washed twice with FACS buffer by spinning at 500G/2 min, then anti-msIgG-PE (Jackson ImmunoResearch, Cat. #115-116-146) was added to the cells, the mixture was incubated for 30 min/4° C. and washed twice with FACS buffer by spinning at 500G/2 min, 1% PFA (Paraformaldehyde) in PBS was added to cell pellet to fix the cells, followed by FACS analysis. ..

    Incubation:

    Article Title: Single domain antibodies against CLL-1
    Article Snippet: .. Briefly, VHH clones (3 μg/ml as final conc./test) in FACS buffer were added to Fc blocked U937 cells (2.5×10{circumflex over ( )}5), the mixtures were incubated for 30 min at 4° C., then washed twice with FACS buffer by spinning at 500G for 2 min; anti-cMyc (Novus, Cat. #NBP2-37822) was added to cells, the mixture was incubated for 30 min/4° C. and washed twice with FACS buffer by spinning at 500G/2 min, then anti-msIgG-PE (Jackson ImmunoResearch, Cat. #115-116-146) was added to the cells, the mixture was incubated for 30 min/4° C. and washed twice with FACS buffer by spinning at 500G/2 min, 1% PFA (Paraformaldehyde) in PBS was added to cell pellet to fix the cells, followed by FACS analysis. ..



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