Review



blocking buffer  (Novus Biologicals)


Bioz Verified Symbol Novus Biologicals is a verified supplier
Bioz Manufacturer Symbol Novus Biologicals manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 95

    Structured Review

    Novus Biologicals blocking buffer
    Blocking Buffer, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 95/100, based on 58 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsa+buffer/CD63+Antibody+(H5C6)+-+BSA+Free/pm41974939-174-25-19
    Average 95 stars, based on 58 article reviews
    blocking buffer - by Bioz Stars, 2026-11
    95/100 stars

    Images

    Related Articles

    Blocking Assay:

    Article Title: Chromosomal instability promotes cell migration and invasion via EFEMP1 secretion into extracellular vesicles.
    Article Snippet: Permeabilization was performed using 0.1% Triton X-100 (Sigma-Aldrich, 9036-19-5) in PBS for 5–10 min at room temperature. .. After blocking with 3% FBS in PBS for 30 min, cells were incubated overnight with a CD63 antibody (H5C6; Novus Biologicals, NBP2-42225) diluted in the blocking buffer. .. Thereafter, cells were washed and incubated with a secondary anti-mouse Alexa Fluor 488 antibody (Invitrogen, A21110) and Phalloidin-iFluor 555 (Abcam, ab176756) for F-actin staining.

    Incubation:

    Article Title: Chromosomal instability promotes cell migration and invasion via EFEMP1 secretion into extracellular vesicles.
    Article Snippet: Permeabilization was performed using 0.1% Triton X-100 (Sigma-Aldrich, 9036-19-5) in PBS for 5–10 min at room temperature. .. After blocking with 3% FBS in PBS for 30 min, cells were incubated overnight with a CD63 antibody (H5C6; Novus Biologicals, NBP2-42225) diluted in the blocking buffer. .. Thereafter, cells were washed and incubated with a secondary anti-mouse Alexa Fluor 488 antibody (Invitrogen, A21110) and Phalloidin-iFluor 555 (Abcam, ab176756) for F-actin staining.

    Article Title: MicroRNA-625-3p Increases Chemosensitivity in Ovarian Cancer Cells Through Decreasing SSX2IP-Mediated Cisplatin Export in Extracellular Vesicles.
    Article Snippet: Proteins were separated on SDS–polyacrylamide gels and electrophoretically transferred to an immobilon polyvinylidene fluoride membrane (EMD Millipore, Billerica, MA, USA). .. The membranes were incubated with Anti-SSX2IP (1:100; HPA027306; SigmaAldrich Co.), anti-CD63 (1:1000; NBP2-42225; Novus Biologicals, Centennial, CO, USA), and β-actin (loading control, 1:5000; clone AC-15, Sigma-Aldrich Co.) overnight at 4 ◦C and then incubated with the appropriate horseradish peroxidase-conjugated secondary https://doi.org/10.3390/cancers18050872 antibodies at a 1:10,000 dilution (Thermo Fisher Scientific) for 1 h at ambient temperature. .. Signals were developed using enhanced chemiluminescence detection reagents (Denville Scientific Inc., Holliston, MA, USA) and visualized on X-ray film (Fujifilm, Tokyo, Japan).

    Control:

    Article Title: MicroRNA-625-3p Increases Chemosensitivity in Ovarian Cancer Cells Through Decreasing SSX2IP-Mediated Cisplatin Export in Extracellular Vesicles.
    Article Snippet: Proteins were separated on SDS–polyacrylamide gels and electrophoretically transferred to an immobilon polyvinylidene fluoride membrane (EMD Millipore, Billerica, MA, USA). .. The membranes were incubated with Anti-SSX2IP (1:100; HPA027306; SigmaAldrich Co.), anti-CD63 (1:1000; NBP2-42225; Novus Biologicals, Centennial, CO, USA), and β-actin (loading control, 1:5000; clone AC-15, Sigma-Aldrich Co.) overnight at 4 ◦C and then incubated with the appropriate horseradish peroxidase-conjugated secondary https://doi.org/10.3390/cancers18050872 antibodies at a 1:10,000 dilution (Thermo Fisher Scientific) for 1 h at ambient temperature. .. Signals were developed using enhanced chemiluminescence detection reagents (Denville Scientific Inc., Holliston, MA, USA) and visualized on X-ray film (Fujifilm, Tokyo, Japan).

    Western Blot:

    Article Title: Chromosomal instability promotes cell migration and invasion via EFEMP1 secretion into extracellular vesicles.
    Article Snippet: Following electrophoresis, proteins were transferred onto PVDF membranes, which were then blocked using Odyssey blocking buffer (LI-COR Biosciences) or 5% non-fat milk in TBS-0.1%T to minimize non-specific binding. .. The primary antibodies for Western blots used in this study were EFEMP1 (1:300, Novus Biologicals, NBP1-77040), β-Actin (1:2000, Cell Signaling Technology, 4970 or 3700S), STAT1 (1:1000, Cell Signaling Technology, 9172S), CD63 (1:1000, Novus Biologicals, NBP2-42225), CD81 (1:1000, Novus Biologicals, NB100-65805SS), vinculin (1:10,000, Sigma-Aldrich, V9131200UL) and Calnexin (1:1000, Novus Biologicals, NB100-1965SS). ..

    other:

    Article Title:
    Article Snippet: Mouse anti-human CD63 (Clone H5C6) , Novus Biologicals , Cat# NBP2-42225; RRID: AB_2884028.



    Similar Products

    96
    Thermo Fisher superblock blocking buffers
    Superblock Blocking Buffers, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsa+buffer/BSA+Blocking+Buffer%2C+3%25+in+TBS/pmc13019076-408-27-30
    Average 96 stars, based on 1 article reviews
    superblock blocking buffers - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    97
    Miltenyi Biotec staining buffer
    Staining Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsa+buffer/MACS+BSA+Stock+Solution/10__1038_slash_s41598___026___69945___x-344-19-25
    Average 97 stars, based on 1 article reviews
    staining buffer - by Bioz Stars, 2026-11
    97/100 stars
      Buy from Supplier

    96
    Elabscience Biotechnology bsa
    Bsa, supplied by Elabscience Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsa+buffer/5%25+Blocking+Buffer/10__5114_slash_ceji_slash_222384-124-6-7
    Average 96 stars, based on 1 article reviews
    bsa - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    97
    Thermo Fisher blocking buffer
    Blocking Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsa+buffer/BSA+blocking+buffer/pm42543390-124-36-38
    Average 97 stars, based on 1 article reviews
    blocking buffer - by Bioz Stars, 2026-11
    97/100 stars
      Buy from Supplier

    97
    Miltenyi Biotec macs buffer
    Macs Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsa+buffer/MACS+BSA+Stock+Solution/bio_rxiv__64898__2026__07__07__736803-387-1-17
    Average 97 stars, based on 1 article reviews
    macs buffer - by Bioz Stars, 2026-11
    97/100 stars
      Buy from Supplier

    97
    Miltenyi Biotec separation buffer
    Separation Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsa+buffer/MACS+BSA+Stock+Solution/pmc13336565-52-14-23
    Average 97 stars, based on 1 article reviews
    separation buffer - by Bioz Stars, 2026-11
    97/100 stars
      Buy from Supplier

    97
    Miltenyi Biotec facs buffer
    Facs Buffer, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsa+buffer/MACS+BSA+Stock+Solution/pmc13349761-121-11-21
    Average 97 stars, based on 1 article reviews
    facs buffer - by Bioz Stars, 2026-11
    97/100 stars
      Buy from Supplier

    96
    Thermo Fisher bsa in pbs
    Bsa In Pbs, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bsa+buffer/BSA+Blocking+Buffer%2C+5%25+in+PBS/pm42348369-98-18-24
    Average 96 stars, based on 1 article reviews
    bsa in pbs - by Bioz Stars, 2026-11
    96/100 stars
      Buy from Supplier

    Image Search Results