Journal: Food Science & Nutrition
Article Title: Exploring the Mechanisms of Total Saponins of Black Ginseng and Ginsenoside Rg3 Against Doxorubicin‐Induced Cardiotoxicity
doi: 10.1002/fsn3.71968
Figure Lengend Snippet: TSF and Rg3 regulate HDAC8/BRCA2/DRP1 pathway inhibits DOX‐induced cardiomyocyte apoptosis. (a) Apoptosis was assessed using TUNEL staining (Scale bar = 50 μm). (b) The results of western blotting for each group. (c) The expression of Bcl‐2 for each group. (d) The expression of DRP1 for each group. (e) The expression of HDAC8 for each group. (f) The expression of BRCA2 for each group. (g) Immunofluorescence staining for observing the colocalization of HDAC8, BRCA2, and DRP1 (Scale bar = 1 mm). Data are expressed as mean ± standard from three individual experiments. Data analysis among groups was performed using ANOVA, with LSD for equal variances and Dunnett’s‐T3 for unequal variances, with significance levels of * p < 0.05, ** p < 0.01, *** p < 0.001.
Article Snippet: Antibodies for BRCA2, DRP1 and Bcl‐2 were sourced from Wanlei Biotechnology Co. Ltd. (Lot: T11052438, T09113028, T11201556), while β ‐actin and HRP‐labeled secondary antibodies were procured from Affinity Biosciences (Lot: T0022, S0001).
Techniques: TUNEL Assay, Staining, Western Blot, Expressing, Immunofluorescence