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anti β tubulin mouse monoclonal antibody (Proteintech)

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Proteintech anti β tubulin mouse monoclonal antibody
Anti β Tubulin Mouse Monoclonal Antibody, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 2517 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/brap/beta+Tubulin+Antibody/pm39155151-258-22-26
Average 96 stars, based on 2517 article reviews

anti β tubulin mouse monoclonal antibody - by Bioz Stars, 2026-10

96/100 stars

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Article Title: Domain-autonomous MKLP1 modules divergently regulate mitotic fidelity and polyploidy in a holocentric system
Article Snippet: rabbit anti-β-tubulin , Proteintech, Wuhan, China , Cat#10094-1-AP;RRID:AB_2210695.

Incubation:

Article Title: Stachyose ameliorates polycystic ovary syndrome through the gut–BAT axis by promoting Alistipes sp. proliferation
Article Snippet: .. Then, proteins were transferred to PVDF membranes followed by incubation in blocking buffer (5% non-fat milk powder/TBS/0.1% Tween-20) at room temperature for 1 h. Anti-β-actin antibody (Proteintech; 66009-1-Ig; dilution, 1:10,000), Anti-GAPDH antibody (CST; 51747; dilution, 1:7,000), Anti-β-tubulin antibody (Proteintech; 10094-1-AP; dilution, 1:5,000), Antiactin antibody (Proteintech; 66009; dilution, 1:5,000), Anti-IOD2 antibody (Immunoway; YN2888; dilution, 1:1,000), Anti-CIDE A antibody (abcam; ab324684; dilution, 1:1,000), anti-UCP1 antibody (Proteintech; 23673-1- AP; dilution 1:1,000), anti-UCP2 antibody (Proteintech; 11081-1-AP; dilution 1:1,000), anti-HSL antibody (SAB; 41039; dilution 1:1,000), antiATGL antibody (Abcam; ab207799; dilution 1:1,000), anti-AMPKα antibody (CST; 2535t; dilution 1:1,000), anti-PGC-1α antibody (Santa Cruz; sc518025; dilution 1:1,000), anti-PGC-1α antibody (Abcam; ab313559; dilution 1:1,000), anti-CYP11A1 antibody (Abcam; ab175408; dilution 1:1,000), anti-CYP17A1 antibody (Abcam; ab134910; dilution 1:1,000), antiHSD3B2 antibody (Abcam; ab191515; dilution 1:1,000), anti-AR antibody (Abcam; ab52615; dilution 1:1,000) were diluted in universal antibody diluent (NCM, WB500D), and immunoblotted the PVDF membranes at 4 °C overnight. .. Then, the PVDF membranes were washed three times for 10min each with TBST, and the HRP-conjugated secondary antibody was npj Biofilms and Microbiomes Article in Press used followedby anECL reagent (Millipore,WBKLS0500) fordetectionand a CCD camera for imaging.

Article Title: Investigating the mechanism of Xiongfu formula and its active ingredient Z-Ligustilide alleviates chronic migraine by modulating the MAPK/ERK/CREB pathway and microglia-mediated neuroinflammation.
Article Snippet: Ethnopharmacological relevance: Chronic migraine is a recurrent throbbing headache, which is often attributed to stagnated Qi and impaired blood circulation from the perspective of traditional Chinese medicine.. Xiongfu formula (CRCR), consisting of Chuanxiong Rhizoma and Cyperi Rhizoma, is a classic herbal formula in treating migraine from the treatise Jingyue Quanshu during the Ming Dynasty.. Aim of the study: This study sought to look at the potential molecular mechanism of CRCR and its active constituents for chronic migraine therapy.

Article Title: Reduced hepatic GLUL expression accompanies altered RIP3/MLKL-pathway protein abundance in early cholestatic liver injury following bile duct ligation
Article Snippet: Equal amounts of protein (20–30 μg) were separated by 10% sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS–PAGE) and transferred onto polyvinylidene difluoride (PVDF) membranes (Millipore, Billerica, MA, United States). .. Membranes were blocked with 5% non-fat milk for 1 h at room temperature and incubated overnight at 4 °C with primary antibodies against glutamine synthetase (GLUL), receptor-interacting protein kinase 3 (RIP3), phospho-RIP3 (p-RIP3), mixed lineage kinase domain-like protein (MLKL), phospho-mixed lineage kinase domain-like protein (p-MLKL), β-tubulin, and GAPDH (Proteintech, Wuhan, China). .. After washing with Tris-buffered saline containing Tween 20 (TBST), membranes were incubated with horseradish peroxidase-conjugated secondary antibodies (Proteintech, Wuhan, China) for 1 h at room temperature.

Blocking Assay:

Binding Assay:

Cell-Signaling:

Recombinant:

Article Title: Gonadotropin‐Inhibitory Hormone (GnIH) and Its Receptor GPR147 Mediates Acute Stress‐Induced Activation of the Locus Coeruleus‐SAM Axis
Article Snippet: In brief, the brainstem and adrenal gland of mice were lysed in RIPA buffer (Cat.89901, Thermo Fisher Scientific Co., Ltd., America). .. The following primary antibodies were used: β‐tubulin (Cat. 66240‐1‐Ig, proteintech, 1:2000 dilution; immunogen: recombinant human Tubulin‐beta protein; species reactivity: mouse), TH (Cat. sc‐25269, Santa Cruz, 1:100 dilution; immunogen: human TH amino acids 1–196; species reactivity: mouse), norepinephrine transporter (NET) (Cat. 680447, Zenbio, 1:1000 dilution; immunogen: a synthetic peptide of human NET; species reactivity: mouse), synaptosomal‐associated protein, 25 kDa (SNAP25) (Cat. R27294 , Zenbio, 1:1000 dilution; immunogen: human SNAP25; species reactivity: mouse), Syntaxin 1A (Cat. R389318, Zenbio, 1:1000 dilution; immunogen: human STX1A; species reactivity: mouse) and Synaptophysin (Cat. R380890, Zenbio, 1:1000 dilution; immunogen: human Synaptophysin amino acids 10–310; species reactivity: mouse). .. After incubation with primary antibodies, the membranes were then incubated with horseradish peroxidase (HRP)‐conjugated goat anti‐rabbit IgG secondary antibody (Cat. 7074P2, 1:20,000 dilution, Cell Signaling Technology) or anti‐mouse IgG secondary antibody (Cat. 7076P2, 1:20,000 dilution, Cell Signaling Technology).

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TSLP expression was increased by down-regulation of BRAP or its mouse homolog. (A) Double immunofluorescence staining by IL-23 and CD11c in skin tissue sections from IMQ treated mice (magnification: 200×); scale bar = 100 μm. Immunostaining with IL-23 antibody was shown as red fluorescence. Immunostaining using antibodies against CD11c was shown as green fluorescence. Nuclei of the cells were localized by DAPI (blue). The orange signals in the merged picture, some of which were shown by red arrows, indicate the co-distribution of IL-23 and CD11c in the cytoplasm of cells. (B) Immunohistochemistry analysis of skin tissue sections using TSLP antibody. Day 0 indicates that the samples were made before the first IMQ application. (C) Daily serum levels of TSLP during the 7-day IMQ treatment as determined by ELISA. (D) Western blot analysis of BRAP expression in HaCaT cells transfected with siRNA to silence C6orf89 expression. (E) ELISA analysis of TSLP content in the culture media of HaCaT cells with C6orf89 expression silenced by siRNA transfection.

Journal: International Journal of Medical Sciences

Article Title: Bombesin receptor-activated protein homolog deficiency altered the pattern of pathological changes of psoriasis - like skin lesion in mice

doi: 10.7150/ijms.89492

Figure Lengend Snippet: TSLP expression was increased by down-regulation of BRAP or its mouse homolog. (A) Double immunofluorescence staining by IL-23 and CD11c in skin tissue sections from IMQ treated mice (magnification: 200×); scale bar = 100 μm. Immunostaining with IL-23 antibody was shown as red fluorescence. Immunostaining using antibodies against CD11c was shown as green fluorescence. Nuclei of the cells were localized by DAPI (blue). The orange signals in the merged picture, some of which were shown by red arrows, indicate the co-distribution of IL-23 and CD11c in the cytoplasm of cells. (B) Immunohistochemistry analysis of skin tissue sections using TSLP antibody. Day 0 indicates that the samples were made before the first IMQ application. (C) Daily serum levels of TSLP during the 7-day IMQ treatment as determined by ELISA. (D) Western blot analysis of BRAP expression in HaCaT cells transfected with siRNA to silence C6orf89 expression. (E) ELISA analysis of TSLP content in the culture media of HaCaT cells with C6orf89 expression silenced by siRNA transfection.

Article Snippet: As illustrated by IHC analysis using an antibody against human BRAP (EPR13621, cat: ab181073, Abcam), BRAP was expressed in various cells from both epidermis and dermis.

Techniques: Expressing, Double Immunofluorescence Staining, Immunostaining, Fluorescence, Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Western Blot, Transfection

The expression of BRAP and its mouse homolog in skin tissues and the skin injury induced by IMQ in both BC004004 -/- mice and their wild type control mice. (A) The expression of BRAP in both normal human skin and the skin of patient with psoriasis by immunohistochemistry analysis of tissue samples with anti-BRAP antibody. BRAP is expressed in the epidermis of both normal skin tissue and skin tissue with psoriasis (magnification: 100×(scale bar = 200 μm) and 400×(scale bar = 50 μm)). (B) BRAP homologous protein was present in skin tissues as revealed by Western blotting in wild-type control mice BC004004 +/+ with imiquimod (IMQ) treatment for 7 day. The total protein extract from one wild type mice skin representing each day of IMQ treatment was loaded in one well. The expression of β-actin from the same sample was used as a loading control. (C) Representative photographs of psoriasis-like lesions on back skin of BC004004 +/+ mice and BC004004 -/- mice after 7 days of IMQ treatment. Vaseline treatment was applied on control groups as a vehicle control. (D) Daily body weights throughout the 7-day IMQ treatment were shown in the upper left panel. The characteristics of skin lesion were depicted as redness, scaling scores and thickness of back skin and scored according to a murine Psoriasis Area and Severity Index (PASI) score table. Data are presented as mean ± SD; n = 9 in each group. * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001.

Journal: International Journal of Medical Sciences

Article Title: Bombesin receptor-activated protein homolog deficiency altered the pattern of pathological changes of psoriasis - like skin lesion in mice

doi: 10.7150/ijms.89492

Figure Lengend Snippet: The expression of BRAP and its mouse homolog in skin tissues and the skin injury induced by IMQ in both BC004004 -/- mice and their wild type control mice. (A) The expression of BRAP in both normal human skin and the skin of patient with psoriasis by immunohistochemistry analysis of tissue samples with anti-BRAP antibody. BRAP is expressed in the epidermis of both normal skin tissue and skin tissue with psoriasis (magnification: 100×(scale bar = 200 μm) and 400×(scale bar = 50 μm)). (B) BRAP homologous protein was present in skin tissues as revealed by Western blotting in wild-type control mice BC004004 +/+ with imiquimod (IMQ) treatment for 7 day. The total protein extract from one wild type mice skin representing each day of IMQ treatment was loaded in one well. The expression of β-actin from the same sample was used as a loading control. (C) Representative photographs of psoriasis-like lesions on back skin of BC004004 +/+ mice and BC004004 -/- mice after 7 days of IMQ treatment. Vaseline treatment was applied on control groups as a vehicle control. (D) Daily body weights throughout the 7-day IMQ treatment were shown in the upper left panel. The characteristics of skin lesion were depicted as redness, scaling scores and thickness of back skin and scored according to a murine Psoriasis Area and Severity Index (PASI) score table. Data are presented as mean ± SD; n = 9 in each group. * p <0.05, ** p <0.01, *** p <0.001, **** p <0.0001.

Article Snippet: The following antibodies and their corresponding dilutions were used: BRAP (Abcam, cat: ab181073; 1:2,000), IL-23 (Santa Cruz, cat: sc-271279,1:1,000;), IL-1β (Santa Cruz, cat: 12742,1:1,000), IL-10 (Santa Cruz, cat: 8438, 1:1,000), IL-17 (Affinity, cat: DF6127, 1:2,000), IL-17 (Santa Cruz, cat: sc-374218, 1:1,000), TNF-α (Santa Cruz, cat: sc-52746, 1:1,000), CK5 (Affinity, cat: AF5479, 1:2,000), β-Actin (Santa Cruz, cat: sc-47778, 1:5,000), CK14 (Santa Cruz, cat: sc-53253, 1:1,000), TGF-β1 (Abcam, cat: ab64715, 1:2,000), HRP-conjugated goat anti-Mouse IgG (H+L) (Proteintech, cat: SA00001-1, 1:5,000), HRP-conjugated goat anti-rabbit IgG(H+L) (SouthernBiotech, cat: 4050-05; 1: 5,000).

Techniques: Expressing, Immunohistochemistry, Western Blot

Journal: International Journal of Medical Sciences

Article Title: Bombesin receptor-activated protein homolog deficiency altered the pattern of pathological changes of psoriasis - like skin lesion in mice

doi: 10.7150/ijms.89492

Techniques: Expressing, Double Immunofluorescence Staining, Immunostaining, Fluorescence, Immunohistochemistry, Enzyme-linked Immunosorbent Assay, Western Blot, Transfection

Figure 2. The analysis of behavioral tests after exposure to chronic unpredictable mild stress (CUMS). (A) Body weight changes throughout the CUMS procedure. (B) Immobility time of bc004004þ/þ and bc004004 / mice in the forced swimming test with treatment of CUMS. (C) Analysis of sucrose preference tests during the CUMS procedure. (D) Immobility time of bc004004þ/þ and bc004004 / mice in the tail suspension test after 21-day CUMS exposure. (E) Total distance traveled in the different regions in the open field test after 21-day CUMS exposure. Two-way ANOVA was performed followed by Tukey’s multiple comparisons. (F) The expres sion of BRAP homologous protein in mice detected by western blot with anti-BRAP antibody. The protein extracts of hippocampus tissues of the mice was used in western blot after 21-day of CUMS treatment. The expression of b-actin from the same sample was used as loading controls. Data are presented as mean ± SD. p <.05, p <.01, p <.001,p <.0001, n ¼ 8.

Journal: Stress (Amsterdam, Netherlands)

Article Title: Lack of bombesin receptor-activated protein homologous protein impairs hippocampal synaptic plasticity and promotes chronic unpredictable mild stress induced behavioral changes in mice.

doi: 10.1080/10253890.2022.2155513

Figure Lengend Snippet: Figure 2. The analysis of behavioral tests after exposure to chronic unpredictable mild stress (CUMS). (A) Body weight changes throughout the CUMS procedure. (B) Immobility time of bc004004þ/þ and bc004004 / mice in the forced swimming test with treatment of CUMS. (C) Analysis of sucrose preference tests during the CUMS procedure. (D) Immobility time of bc004004þ/þ and bc004004 / mice in the tail suspension test after 21-day CUMS exposure. (E) Total distance traveled in the different regions in the open field test after 21-day CUMS exposure. Two-way ANOVA was performed followed by Tukey’s multiple comparisons. (F) The expres sion of BRAP homologous protein in mice detected by western blot with anti-BRAP antibody. The protein extracts of hippocampus tissues of the mice was used in western blot after 21-day of CUMS treatment. The expression of b-actin from the same sample was used as loading controls. Data are presented as mean ± SD. p <.05, p <.01, p <.001,p <.0001, n ¼ 8.

Article Snippet: The transferred PVDF membranes were incubated with the following primary antibodies with dilutions indicated respectively: b-actin (Sigma, cat: A5441, 1:10,000), BDNF (Abcam, cat: ab108319, 1:1000), Trk-B (Boster, cat: BA0623, 1:1000), BRAP (Abcam, cat: ab181073, 1:1000), GluN2A (CUSABIO, cat: CSB-PA14129A0Rb, 1:500), PSD95 (Sangon Biotech, cat: D261092, 1:500), synaptophysin (ABclonal, cat: A6344, 1:1000).

Techniques: Suspension, Western Blot, Expressing

Journal: Stress (Amsterdam, Netherlands)

doi: 10.1080/10253890.2022.2155513

Techniques: Suspension, Western Blot, Expressing

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anti β tubulin mouse monoclonal antibody (Proteintech)

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