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brainphys neuronal medium without phenol red (STEMCELL Technologies Inc)

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STEMCELL Technologies Inc brainphys neuronal medium without phenol red
Brainphys Neuronal Medium Without Phenol Red, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/brainphys+medium/brainphys+phenol+red/pm40540399-337-16-18
Average 90 stars, based on 1 article reviews

brainphys neuronal medium without phenol red - by Bioz Stars, 2026-11

90/100 stars

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Recombinant:

Article Title: A protocol for isolation and culturing of mouse primary postmitotic photoreceptors and isolation of extracellular vesicles.
Article Snippet: .. STAR Protocols 5, 102875, March 15, 2024 5 Photoreceptor chemically defined medium Final concentrations Volume BrainPhys Neuronal Medium no phenol red STEMCELL Technologies Inc. Up to 50 mL 45.65 mL HEPES (1 M) 15 mM 0.750 mL 1003 ITS (1.0 mg/mL recombinant human insulin, 0.55 mg/mL human transferrin (substantially iron-free), and 0.5 mg/mL sodium selenite) Diluted from 1003 to 13 0.500 mL Taurine (50 mM) 1 mM 1 mL BSA (30%) 1% 1.6 mL Antibiotics Pen/ Strep 1000 mg/ml (Sigma) Diluted from 1003 to 13 0.500 mL *Aliquot Photoreceptor medium and store at 20 C, medium should be used within 1 week if kept at 4 C. Use within a week once complete medium is prepared. .. Dissection buffer Final concentrations Volume DMEM/F12 Up to 50 mL 48.20 mL HEPES (1 M) 15 mM 0.750 mL Taurine (50 mM) 1 mM 1 mL DNase (from Worthington kit) 0.5 U/mL 0.05 mL *Aliquot and store at 20 C, medium should be used within 1 week if kept at 4 C. Use within a week once thawed.

other:

Article Title: Single-nucleus multi-omics identifies shared and distinct pathways in Pick’s and Alzheimer’s disease
Article Snippet: UBE3A mutant and parental lines were transfected via Nucleofection (LONZA Cat #VPH-5022) of the PB-TO-hNGN2 (Addgene Cat #172115*) plasmid and purified in the presence of 200 ng/mL Puromycin (Invivogen ant-pr-1) until the majority of cells showed plasmid expression as determined by BFP expression.

Article Title: High-content analysis and Kinetic Image Cytometry identify toxicity and epigenetic effects of HIV antiretrovirals on human iPSC-neurons and primary neural precursor cells.
Article Snippet: Introduction: Despite viral suppression due to combination antiretroviral therapy (cART), HIV-associated neurocognitive disorders (HAND) continue to affect half of people with HIV, suggesting that certain antiretrovirals (ARVs) may contribute to HAND.. Methods: We examined the effects of nucleoside/nucleotide reverse transcriptase inhibitors tenofovir disoproxil fumarate (TDF) and emtricitabine (FTC) and the integrase inhibitors dolutegravir (DTG) and elvitegravir (EVG) on viability, structure, and function of glutamatergic neurons (a subtype of CNS neuron involved in cognition) derived from human induced pluripotent stem cells (hiPSC-neurons), and primary human neural precursor cells (hNPCs), which are responsible for neurogenesis.. Results: Using automated digital microscopy and image analysis (high content analysis, HCA), we found that DTG, EVG, and TDF decreased hiPSC-neuron viability, neurites, and synapses after 7 days of treatment.

Transfection:

Article Title: NMDA receptor autoantibodies primarily impair the extrasynaptic compartment.
Article Snippet: .. The medium was changed during transfection to BrainPhysTM medium without Phenol Red (Stemcell, #05791) supplemented with 1× B-27TM plus supplement. ..

Article Title: NMDA receptor autoantibodies primarily impair the extrasynaptic compartment
Article Snippet: .. The medium was changed during transfection to BrainPhys TM medium without Phenol Red (Stemcell, #05791) supplemented with 1× B-27 plus supplement. ..

Fluorescence:

Article Title: Human neuromuscular junction on micro-structured microfluidic devices implemented with a custom micro electrode array (MEA).
Article Snippet: .. When Ca2+ binds to the calmodulin domain, it triggers a change of conformation producing detectable fluorescence.45 Co-culture was differentiated in 20b4 microfluidic devices as described above and equilibrated in BrainPhys medium without phenol red (StemCell Technologies) prior recording. ..