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brainphys medium supplemented with sm1 (stemcell technologies, #05792)  (STEMCELL Technologies Inc)

 
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    STEMCELL Technologies Inc brainphys medium supplemented with sm1 (stemcell technologies, #05792)
    Brainphys Medium Supplemented With Sm1 (Stemcell Technologies, #05792), supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/brainphys+medium/brainphys+plus+sm1/pm40536248-64-16-17
    Average 90 stars, based on 1 article reviews
    brainphys medium supplemented with sm1 (stemcell technologies, #05792) - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: Swift induction of human spinal lower motor neurons and robust ALS cell screening via single-cell imaging.
    Article Snippet: Quantitative reverse-transcription PCR was performed using Premix Ex Taq II (Takara Bio Inc., Shiga, Japan) on a ViiA 7 real-time PCR system (Thermo Fisher Stem Cell Reports j Vol.

    Article Title: OAS1: A Protective Mechanism for Alzheimer's Disease? An Exploration of Data and Possible Mechanisms.
    Article Snippet: After incubation at room temperature overnight, slides were imaged using a Zeiss LSM 780 confocal microscope (Oberkochen, Germany).

    Article Title: An antisense oligonucleotide-based strategy to ameliorate cognitive dysfunction in the 22q11.2 Deletion Syndrome
    Article Snippet: On day 0, lentiviruses were added in fresh basic media, containing always DMEM/F12 (catalog#11330032, Thermo Fisher Scientific, Waltham, MA, USA), human BDNF (10 ng/ml), human NT-3 (10 ng/ml, catalog#450–03, PeproTech, East Windsor, NJ, USA), mouse laminin (0.1 μg/ml, catalog#354232, Corning, NY, USA), N2 supplement, B27 plus supplement, non-essential amino acids (NEAA, catalog#SH30238.01, Cytiva, Marlborough, MA, USA) supplement and doxycycline (1 mg/ml, catalog#D9891-1G, Sigma-Aldrich, St. Louis, MO, USA) to induce TetO gene.

    In Vitro:

    Article Title: Monitoring of activity-driven trafficking of endogenous synaptic proteins through proximity labeling.
    Article Snippet: After 5 days in vitro, the culture medium was partially replaced with BrainPhys Neuronal Medium supplemented with 2% SM1 (STEMCELL Technologies, 05792), 12.5 mM Glucose, and 10 μm 50-fluoro-20-deoxyuridine (FUDR; Thermo Fisher Scientific, 10144760), an antimitotic drug that suppresses growth of glial cells. .. After 5 days in vitro, the culture medium was partially replaced with BrainPhys Neuronal Medium supplemented with 2% SM1 (STEMCELL Technologies, 05792), 12.5 mM Glucose, and 10 μm 50-fluoro-20-deoxyuridine (FUDR; Thermo Fisher Scientific, 10144760), an antimitotic drug that suppresses growth of glial cells. .. Pregnant wild-type Sprague Dawley Crl:CD (SD) rats were purchased from Charles River Laboratories.

    Article Title: Monitoring of activity-driven trafficking of endogenous synaptic proteins through proximity labeling
    Article Snippet: Lastly, 4 million neurons were plated per 10 cm dish, which were previously coated with 0.1 mg/ml poly-D-lysine (Merck, P2636). .. After 5 days in vitro, the culture medium was partially replaced with BrainPhys Neuronal Medium supplemented with 2% SM1 (STEMCELL Technologies, 05792), 12.5 mM Glucose, and 10 μM 5′-fluoro-2′-deoxyuridine (FUDR; Thermo Fisher Scientific, 10144760), an antimitotic drug that suppresses growth of glial cells. ..

    Cell Culture:

    Article Title: Low-Glucose Culture Conditions Bias Neuronal Energetics Towards Oxidative Phosphorylation.
    Article Snippet: .. In a subset of experiments described below, we cultured neuronal cells in BrainPhys medium supplemented with SM1 (StemCell Technologies, #05792) following media preparation and media exchange as described in Faria- Pereira et al. (2022). .. In a subset of experiments described below, we cultured neuronal cells in BrainPhys medium supplemented with SM1 (StemCell Technologies, #05792) following media preparation and media exchange as described in Faria- Pereira et al. (2022).

    Article Title: Low‐Glucose Culture Conditions Bias Neuronal Energetics Towards Oxidative Phosphorylation
    Article Snippet: .. In a subset of experiments described below, we cultured neuronal cells in BrainPhys medium supplemented with SM1 (StemCell Technologies, #05792) following media preparation and media exchange as described in Faria‐Pereira et al. ( ). .. To measure cell viability, inferred from cellular reductive capacity, culture medium was replaced with fresh medium containing 10% (v/v, 1X) alamarBlue Cell Viability Reagent (ThermoFisher; #DAL1100) and stored in an incubator for 3 h. Then, 570 nm absorbance was measured on a multi‐well plate reader (RRID:SCR_020300, SpectraMax M5 Multi‐Mode Microplate Reader, Molecular Devices, San Jose, CA, US) and corrected for 600 nm absorbance, as per the protocol provided by the manufacturer.



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