brainphys imaging optimized medium (biom) (STEMCELL Technologies Inc)
90
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STEMCELL Technologies Inc
brainphys imaging optimized medium (biom)
Brainphys Imaging Optimized Medium (Biom), supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/brainphys+medium/brainphys+imaging+optimized+medium/pmc10171804-685-5-10
Average 90 stars, based on 1 article reviews
Brainphys Imaging Optimized Medium (Biom), supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/brainphys+medium/brainphys+imaging+optimized+medium/pmc10171804-685-5-10
Average 90 stars, based on 1 article reviews
brainphys imaging optimized medium (biom) - by Bioz Stars,
2026-11
90/100 stars
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Imaging:Article Title: Synaptic deregulation of cholinergic projection neurons causes olfactory dysfunction across five fly Parkinsonism models Article Snippet: .. For calcium imaging, cell culture medium was switched to Article Title: Sporadic ALS iPSC-derived motor neurons show axonal defects linked to altered axon guidance pathways. Article Snippet: .. Axonal transport detection and analysis Motor neurons (DIV 22, 30, and 39) from sALS patients and controls were stained with MitoTrackergreen (50 nM, Invitrogen, Cat N° M7514) for 20 min and then washed and kept in Article Title: A 3D human iPSC-derived multi-cell type neurosphere system to model cellular responses to chronic amyloidosis Article Snippet: .. Patch-clamp recordings on microglial cells were performed on hiNS(+) either ctrl or Aβ (3w/5w) treated after plating them on coverslips, which allowed moving them to a 2-photon microscope for patch-clamp and image recordings (Zeiss 510Meta microscope with a Ti:Sapphire Chameleon laser). hiMG were stained with 2 μL tomato-lectin- 488 (for patch-clamp) or 594 (for process motility quantification) (Thermo Scientific, L32470 and L32471, respectively) per well for 30 min to allow for the identification of microglia in neurospheres using the 2-photon microscope. hiNS were supplied with a continuous perfusion of carbogen (95% O 2 /5% CO 2 ) infused Article Title: TDP43 autoregulation gives rise to dominant negative isoforms that are tightly controlled by transcriptional and post-translational mechanisms Article Snippet: .. At in vitro day (DIV) 4, neurons were transfected with 100 ng of a control fluorescent plasmid to mark cell bodies and 100 ng of an experimental construct using Lipofectamine 2000 as previously described., , , , Following transfection, cells were placed in either Neurobasal Complete Media (Neurobasal [Gibco 21103-049], 1x B27 Supplement [Gibco, 17504-044], 1x Glutamax, 100 units/mL Pen Strep), NEUMO photostable medium with SOS (Cell Guidance Systems, M07-500), or Article Title: Synaptic deregulation of cholinergic projection neurons causes olfactory dysfunction across 5 fly Parkinsonism models Article Snippet: .. For calcium imaging, cell culture medium was switched to Article Title: Single-cell RNA-sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS - and TARDBP -ALS Article Snippet: .. For tracking mitochondria, motor neurons were cultured in Article Title: A 3D human iPSC-derived multi-cell type neurosphere system to model cellular responses to chronic amyloidosis. Article Snippet: .. Patch-clamp recordings on microglial cells were performed on hiNS(+) either ctrl or Aβ (3w/5w) treated after plating them on coverslips, which allowed moving them to a 2-photon microscope for patch-clamp and image recordings (Zeiss 510Meta microscope with a Ti:Sapphire Chameleon laser). hiMG were stained with 2 μL tomato-lectin- 488 (for patch-clamp) or 594 (for process motility quantification) (Thermo Scientific, L32470 and L32471, respectively) per well for 30 min to allow for the identification of microglia in neurospheres using the 2-photon microscope. hiNS were supplied with a continuous perfusion of carbogen (95% O2/5% CO2) infused Cell Culture:Article Title: Synaptic deregulation of cholinergic projection neurons causes olfactory dysfunction across five fly Parkinsonism models Article Snippet: .. For calcium imaging, cell culture medium was switched to Article Title: Synaptic deregulation of cholinergic projection neurons causes olfactory dysfunction across 5 fly Parkinsonism models Article Snippet: .. For calcium imaging, cell culture medium was switched to Article Title: Single-cell RNA-sequencing reveals early mitochondrial dysfunction unique to motor neurons shared across FUS - and TARDBP -ALS Article Snippet: .. For tracking mitochondria, motor neurons were cultured in Staining:Article Title: Sporadic ALS iPSC-derived motor neurons show axonal defects linked to altered axon guidance pathways. Article Snippet: .. Axonal transport detection and analysis Motor neurons (DIV 22, 30, and 39) from sALS patients and controls were stained with MitoTrackergreen (50 nM, Invitrogen, Cat N° M7514) for 20 min and then washed and kept in Article Title: A 3D human iPSC-derived multi-cell type neurosphere system to model cellular responses to chronic amyloidosis Article Snippet: .. Patch-clamp recordings on microglial cells were performed on hiNS(+) either ctrl or Aβ (3w/5w) treated after plating them on coverslips, which allowed moving them to a 2-photon microscope for patch-clamp and image recordings (Zeiss 510Meta microscope with a Ti:Sapphire Chameleon laser). hiMG were stained with 2 μL tomato-lectin- 488 (for patch-clamp) or 594 (for process motility quantification) (Thermo Scientific, L32470 and L32471, respectively) per well for 30 min to allow for the identification of microglia in neurospheres using the 2-photon microscope. hiNS were supplied with a continuous perfusion of carbogen (95% O 2 /5% CO 2 ) infused Article Title: A 3D human iPSC-derived multi-cell type neurosphere system to model cellular responses to chronic amyloidosis. Article Snippet: .. Patch-clamp recordings on microglial cells were performed on hiNS(+) either ctrl or Aβ (3w/5w) treated after plating them on coverslips, which allowed moving them to a 2-photon microscope for patch-clamp and image recordings (Zeiss 510Meta microscope with a Ti:Sapphire Chameleon laser). hiMG were stained with 2 μL tomato-lectin- 488 (for patch-clamp) or 594 (for process motility quantification) (Thermo Scientific, L32470 and L32471, respectively) per well for 30 min to allow for the identification of microglia in neurospheres using the 2-photon microscope. hiNS were supplied with a continuous perfusion of carbogen (95% O2/5% CO2) infused Patch Clamp:Article Title: A 3D human iPSC-derived multi-cell type neurosphere system to model cellular responses to chronic amyloidosis Article Snippet: .. Patch-clamp recordings on microglial cells were performed on hiNS(+) either ctrl or Aβ (3w/5w) treated after plating them on coverslips, which allowed moving them to a 2-photon microscope for patch-clamp and image recordings (Zeiss 510Meta microscope with a Ti:Sapphire Chameleon laser). hiMG were stained with 2 μL tomato-lectin- 488 (for patch-clamp) or 594 (for process motility quantification) (Thermo Scientific, L32470 and L32471, respectively) per well for 30 min to allow for the identification of microglia in neurospheres using the 2-photon microscope. hiNS were supplied with a continuous perfusion of carbogen (95% O 2 /5% CO 2 ) infused Article Title: A 3D human iPSC-derived multi-cell type neurosphere system to model cellular responses to chronic amyloidosis. Article Snippet: .. Patch-clamp recordings on microglial cells were performed on hiNS(+) either ctrl or Aβ (3w/5w) treated after plating them on coverslips, which allowed moving them to a 2-photon microscope for patch-clamp and image recordings (Zeiss 510Meta microscope with a Ti:Sapphire Chameleon laser). hiMG were stained with 2 μL tomato-lectin- 488 (for patch-clamp) or 594 (for process motility quantification) (Thermo Scientific, L32470 and L32471, respectively) per well for 30 min to allow for the identification of microglia in neurospheres using the 2-photon microscope. hiNS were supplied with a continuous perfusion of carbogen (95% O2/5% CO2) infused Microscopy:Article Title: A 3D human iPSC-derived multi-cell type neurosphere system to model cellular responses to chronic amyloidosis Article Snippet: .. Patch-clamp recordings on microglial cells were performed on hiNS(+) either ctrl or Aβ (3w/5w) treated after plating them on coverslips, which allowed moving them to a 2-photon microscope for patch-clamp and image recordings (Zeiss 510Meta microscope with a Ti:Sapphire Chameleon laser). hiMG were stained with 2 μL tomato-lectin- 488 (for patch-clamp) or 594 (for process motility quantification) (Thermo Scientific, L32470 and L32471, respectively) per well for 30 min to allow for the identification of microglia in neurospheres using the 2-photon microscope. hiNS were supplied with a continuous perfusion of carbogen (95% O 2 /5% CO 2 ) infused Article Title: A 3D human iPSC-derived multi-cell type neurosphere system to model cellular responses to chronic amyloidosis. Article Snippet: .. Patch-clamp recordings on microglial cells were performed on hiNS(+) either ctrl or Aβ (3w/5w) treated after plating them on coverslips, which allowed moving them to a 2-photon microscope for patch-clamp and image recordings (Zeiss 510Meta microscope with a Ti:Sapphire Chameleon laser). hiMG were stained with 2 μL tomato-lectin- 488 (for patch-clamp) or 594 (for process motility quantification) (Thermo Scientific, L32470 and L32471, respectively) per well for 30 min to allow for the identification of microglia in neurospheres using the 2-photon microscope. hiNS were supplied with a continuous perfusion of carbogen (95% O2/5% CO2) infused In Vitro:Article Title: TDP43 autoregulation gives rise to dominant negative isoforms that are tightly controlled by transcriptional and post-translational mechanisms Article Snippet: .. At in vitro day (DIV) 4, neurons were transfected with 100 ng of a control fluorescent plasmid to mark cell bodies and 100 ng of an experimental construct using Lipofectamine 2000 as previously described., , , , Following transfection, cells were placed in either Neurobasal Complete Media (Neurobasal [Gibco 21103-049], 1x B27 Supplement [Gibco, 17504-044], 1x Glutamax, 100 units/mL Pen Strep), NEUMO photostable medium with SOS (Cell Guidance Systems, M07-500), or Transfection:Article Title: TDP43 autoregulation gives rise to dominant negative isoforms that are tightly controlled by transcriptional and post-translational mechanisms Article Snippet: .. At in vitro day (DIV) 4, neurons were transfected with 100 ng of a control fluorescent plasmid to mark cell bodies and 100 ng of an experimental construct using Lipofectamine 2000 as previously described., , , , Following transfection, cells were placed in either Neurobasal Complete Media (Neurobasal [Gibco 21103-049], 1x B27 Supplement [Gibco, 17504-044], 1x Glutamax, 100 units/mL Pen Strep), NEUMO photostable medium with SOS (Cell Guidance Systems, M07-500), or Control:Article Title: TDP43 autoregulation gives rise to dominant negative isoforms that are tightly controlled by transcriptional and post-translational mechanisms Article Snippet: .. At in vitro day (DIV) 4, neurons were transfected with 100 ng of a control fluorescent plasmid to mark cell bodies and 100 ng of an experimental construct using Lipofectamine 2000 as previously described., , , , Following transfection, cells were placed in either Neurobasal Complete Media (Neurobasal [Gibco 21103-049], 1x B27 Supplement [Gibco, 17504-044], 1x Glutamax, 100 units/mL Pen Strep), NEUMO photostable medium with SOS (Cell Guidance Systems, M07-500), or Plasmid Preparation:Article Title: TDP43 autoregulation gives rise to dominant negative isoforms that are tightly controlled by transcriptional and post-translational mechanisms Article Snippet: .. At in vitro day (DIV) 4, neurons were transfected with 100 ng of a control fluorescent plasmid to mark cell bodies and 100 ng of an experimental construct using Lipofectamine 2000 as previously described., , , , Following transfection, cells were placed in either Neurobasal Complete Media (Neurobasal [Gibco 21103-049], 1x B27 Supplement [Gibco, 17504-044], 1x Glutamax, 100 units/mL Pen Strep), NEUMO photostable medium with SOS (Cell Guidance Systems, M07-500), or Construct:Article Title: TDP43 autoregulation gives rise to dominant negative isoforms that are tightly controlled by transcriptional and post-translational mechanisms Article Snippet: .. At in vitro day (DIV) 4, neurons were transfected with 100 ng of a control fluorescent plasmid to mark cell bodies and 100 ng of an experimental construct using Lipofectamine 2000 as previously described., , , , Following transfection, cells were placed in either Neurobasal Complete Media (Neurobasal [Gibco 21103-049], 1x B27 Supplement [Gibco, 17504-044], 1x Glutamax, 100 units/mL Pen Strep), NEUMO photostable medium with SOS (Cell Guidance Systems, M07-500), or Incubation:Article Title: TDP43 autoregulation gives rise to dominant negative isoforms that are tightly controlled by transcriptional and post-translational mechanisms Article Snippet: .. At in vitro day (DIV) 4, neurons were transfected with 100 ng of a control fluorescent plasmid to mark cell bodies and 100 ng of an experimental construct using Lipofectamine 2000 as previously described., , , , Following transfection, cells were placed in either Neurobasal Complete Media (Neurobasal [Gibco 21103-049], 1x B27 Supplement [Gibco, 17504-044], 1x Glutamax, 100 units/mL Pen Strep), NEUMO photostable medium with SOS (Cell Guidance Systems, M07-500), or |