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Meso Scale Diagnostics LLC msd platform
Msd Platform, supplied by Meso Scale Diagnostics LLC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bovine+microarray+platform/msd+platform/pm22621944-18-40-24
Average 90 stars, based on 1 article reviews
msd platform - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Enzyme-linked Immunosorbent Assay:

Article Title: Anti-TIGIT antibodies
Article Snippet: Where A=31282-PE %31282 at concentration x and B=31282-PE %31282 untreated for the detection with this clone, while for detection with clone 32959-AF647, A=32959-AF647*%31282 at concentration x and B=32959-AF647%31282 untreated. .. The ELISA assay is based on the Meso Scale Discovery (MSD) Platform using electrochemiluminescence detection technology. ..

Electrochemiluminescence:

Article Title: Anti-TIGIT antibodies
Article Snippet: Where A=31282-PE %31282 at concentration x and B=31282-PE %31282 untreated for the detection with this clone, while for detection with clone 32959-AF647, A=32959-AF647*%31282 at concentration x and B=32959-AF647%31282 untreated. .. The ELISA assay is based on the Meso Scale Discovery (MSD) Platform using electrochemiluminescence detection technology. ..

Article Title: Anti-TREM2 antibodies and methods of use thereof
Article Snippet: .. For anti-TREM2 antibody PK analysis, the total antibody concentrations in monkey plasma were quantified using a generic anti-human IgG sandwich electrochemiluminescence immunoassay (ECLIA) on a Meso Scale Discovery (MSD) platform. .. Briefly, 1% casein-based PBS blocking buffer (Thermo Scientific, MA) was added to an MSD GOLD 96-well small-spot streptavidin-coated microtiter plate (Meso Scale Discovery, MD) and incubated for approximately 1 hr.

other:

Article Title: Antiretroviral Therapy at Conception Leads to Lower Peripheral CD49a + NK Cells and Higher SERPINB2
Article Snippet: For this analysis, we used both the Luminex and the Mesoscale platform (MSD) with custom multiplex panels that we had previously validated for sensitivity and accuracy on a different set of samples.

Article Title: Anti-TCR antibody molecules and uses thereof
Article Snippet: Cytokines (IFNγ, IL-2, TNFα, IL-1β or IL-6) were measured using MSD technology platform (MesoScale Discovery) as described in the manufacturer's protocol.

Clinical Proteomics:

Article Title: Anti-TREM2 antibodies and methods of use thereof
Article Snippet: .. For anti-TREM2 antibody PK analysis, the total antibody concentrations in monkey plasma were quantified using a generic anti-human IgG sandwich electrochemiluminescence immunoassay (ECLIA) on a Meso Scale Discovery (MSD) platform. .. Briefly, 1% casein-based PBS blocking buffer (Thermo Scientific, MA) was added to an MSD GOLD 96-well small-spot streptavidin-coated microtiter plate (Meso Scale Discovery, MD) and incubated for approximately 1 hr.

Article Title: Immunomodulating urea azalides
Article Snippet: .. To collect plasma, plates were centrifuged at 2,000×g for 10 minutes and 100 μL of supernatant was transferred to a 96 well polypropylene plate that was sealed and stored at −80° C. Samples were assayed for cytokines using the Meso Scale Discovery (MSD) platform (described below) from plasma collected directly from drug treated animals as well as plasma collected from the whole blood assay. .. Cytokine Detection—An MSD U-Plex cytokine assay was developed following the MSD U-Plex protocol guide and included biotinylated capture antibodies against: bovine Interleukin (IL)-1β (Biorad), bovine IL-6 (R&D Systems) and bovine tumor necrosis factor (TNF)-α (R&D Systems).

Article Title: Murine modeling of menstruation identifies immune correlates of protection during Chlamydia muridarum challenge.
Article Snippet: .. CVL supernatant and blood plasma were measured and analyzed for cytokine/chemokines through the Emory Multiplexed Immunoassay Core using the Meso Scale Discovery (MSD) platform using a murine and NHP multiplex assay kit in one batch run per species. ..

Whole Blood Assay:

Article Title: Immunomodulating urea azalides
Article Snippet: .. To collect plasma, plates were centrifuged at 2,000×g for 10 minutes and 100 μL of supernatant was transferred to a 96 well polypropylene plate that was sealed and stored at −80° C. Samples were assayed for cytokines using the Meso Scale Discovery (MSD) platform (described below) from plasma collected directly from drug treated animals as well as plasma collected from the whole blood assay. .. Cytokine Detection—An MSD U-Plex cytokine assay was developed following the MSD U-Plex protocol guide and included biotinylated capture antibodies against: bovine Interleukin (IL)-1β (Biorad), bovine IL-6 (R&D Systems) and bovine tumor necrosis factor (TNF)-α (R&D Systems).

Cell Culture:

Article Title: Generation of cynomolgus monkey capsid-specific positive control cells for IFNγ ELISpot assays for adeno-associated gene therapy applications
Article Snippet: .. Secreted cytokines and chemokines analysis Cytokines and chemokines in the cell culture supernatants were analyzed on a Meso Scale Discovery (MSD) platform using customized multiplex assays (Rockville, MD). ..

Multiplex Assay:

Article Title: Generation of cynomolgus monkey capsid-specific positive control cells for IFNγ ELISpot assays for adeno-associated gene therapy applications
Article Snippet: .. Secreted cytokines and chemokines analysis Cytokines and chemokines in the cell culture supernatants were analyzed on a Meso Scale Discovery (MSD) platform using customized multiplex assays (Rockville, MD). ..

Article Title: Murine modeling of menstruation identifies immune correlates of protection during Chlamydia muridarum challenge.
Article Snippet: .. CVL supernatant and blood plasma were measured and analyzed for cytokine/chemokines through the Emory Multiplexed Immunoassay Core using the Meso Scale Discovery (MSD) platform using a murine and NHP multiplex assay kit in one batch run per species. ..



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Microarray analysis and validation of KDM4D mRNA expression in bMECs stimulated with Mycoplasma bovis ; bovine mammary epithelial cells (bMECs) were incubated with live M. bovis at an multiplicity of infection of 1,000 for 6 h. The data are expressed from three (microarray) or four (real-time polymerase chain reaction [PCR]) independent experiments. Significant difference ( * p <0.05) compared with unstimulated bMECs.

Journal: Journal of Veterinary Science

Article Title: Innate immune response of bovine mammary epithelial cells to Mycoplasma bovis

doi: 10.4142/jvs.2018.19.1.79

Figure Lengend Snippet: Microarray analysis and validation of KDM4D mRNA expression in bMECs stimulated with Mycoplasma bovis ; bovine mammary epithelial cells (bMECs) were incubated with live M. bovis at an multiplicity of infection of 1,000 for 6 h. The data are expressed from three (microarray) or four (real-time polymerase chain reaction [PCR]) independent experiments. Significant difference ( * p <0.05) compared with unstimulated bMECs.

Article Snippet: The gene expression dataset was obtained via Agilent single-color microarray platform (4 × 44K bovine gene expression array, grid ID 023647).

Techniques: Microarray, Expressing, Incubation, Infection, Real-time Polymerase Chain Reaction