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bmp 4 protein  (R&D Systems)


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    Structured Review

    R&D Systems bmp 4 protein
    Bmp 4 Protein, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 124 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bmp4/Recombinant+Mouse+BMP-4+Protein/pm40136675-48-9-14
    Average 95 stars, based on 124 article reviews
    bmp 4 protein - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Incubation:

    Article Title: An ancient enhancer rapidly evolving in the human lineage promotes neural development and cognitive flexibility
    Article Snippet: .. After 24 hours, the media were replaced with DMEM F12/RPMI (1:1) medium (Gibco) supplemented with NaHCO 3 (2.5 μg/ml; Sigma-Aldrich), 1% GlutaMAX (Gibco), glucose (5.5 mM; Sigma-Aldrich), 0.1% fatty acid free bovine serum albumin (BSA) (Sigma-Aldrich), and insulin-transferrin-selenium-ethanolamine supplement (ITS-X; 1:100; Gibco) supplemented with wingless-type MMTV integration site family, member 3A (WNT3A; 20 ng/ml), bone morphogenetic protein 4 (BMP4) (10 ng/ml; R&D Systems), and activin A (AA; 100 ng/ml) for 1 day, followed by incubation for 3 days with the same media containing AA (100 ng/ml) only. ..

    Recombinant:

    Article Title: Brachiopod genome unveils the evolution of BMP signalling in bilaterian body patterning.
    Article Snippet: .. Exogenous recombinant mouse BMP4 (mBMP4; R&D Systems 5020-BP) was used to overactivate the BMP pathway88. ..

    Article Title: YAP regulates transcriptional programs for layer-specific periosteal expansion during fracture repair
    Article Snippet: As the proteins we were probing for were all in the range of 50 to 60 kDa, we used a light chain–specific, horseradish peroxidase–conjugated secondary antibody for detection (cat. no. 45262, Cell Signaling Technology). .. Recombinant mouse BMP4 (cat. no. 5020-BP, R&D Systems, Minneapolis, MN) was reconstituted in 0.9% sterile saline solution (cat. no. S5825, Teknova, Hollister, CA) at 12.5 μg/ml. ..

    Article Title: Cellular quiescence uncouples the proteome from the transcriptome in neural stem cells.
    Article Snippet: .. NSCs were then propagated in Dulbecco’s modified Eagle’s medium (DMEM)/F12 with Glutamax (Thermo Fischer Scientific 31331093)+N-2 MAX supplement (R&D Systems AR009) + penicillin–streptomycin (Thermo Fischer Scientific 15140)+ 2 mg/ml laminin (Sigma L2020)+ 5 mg/ml heparin (Sigma H3393-50KU)+ 20 ng/ml recombinant murine fibroblast growth factor 2 (FGF-2) (PeproTech 450-33)+ 20 ng/ml recombinant murine epidermal growth factor (EGF) (PeproTech 315-09) in tissue culture incubators at 37 °C with 5% CO2. qNSC induction was performed with 50 ng/ml BMP4 (R&D Systems 5020-BP-010) and qNSCs cultures were never passaged. .. Mouse cells were fixed in 4% paraformaldehyde (PFA) (Thermo Fisher Scientific J61899.AK) in PBS for 10 min at RT, washed in PBS, then stored 0.02% sodium azide in PBS at 4 °C.

    Article Title: Acute regulation of murine adipose tissue lipolysis and insulin resistance by the TGFβ superfamily protein GDF3
    Article Snippet: .. Recombinant proteins Recombinants Species Catalog No Company Used for GDF3 Mouse 9009-GD-010 R&D Systems Flow Cytometry, BLI, ITC, luciferase reporter assay, lipolysis GDF3 Human 5754-G3-010 R&D Systems BLI GDF3 Human 5754-G3-010/CF R&D Systems SPR BMP2 Human N/A Vicki Rosen lab Flow Cytometry, lipolysis BMP2 Human/ Mouse/ Rat 120-02C-10UG PeproTech ITC, lipolysis BMP4 Mouse 5020-BP-010 R&D Systems ITC, lipolysis BMP7 Mouse 5666-BP-010 R&D Systems Flow Cytometry BMP9 Human 3209-BP-010 R&D Systems Flow Cytometry, lipolysis BMP10 Mouse 6038-BP-025 R&D Systems Flow Cytometry, lipolysis TGFβ1 Human T7039 Millipore Sigma Flow Cytometry, lipolysis BMPRII-Fc Human/ Mouse 811-BR-100 R&D Systems SPR, BLI, ITC TGFBRII-Fc Human 341-BR-050/CF R&D Systems SPR ACTRIIA-Fc Human 340-R2-100/CF R&D Systems SPR ACTRIIB-Fc Human 339-RB-100/CF R&D Systems SPR IgG-Fc Human 160024 Gator Bio BLI IgG-Fc Mouse 160004 Gator Bio BLI Table S2. ..

    Article Title: Brachiopod genome unveils the evolution of BMP signalling in bilaterian body patterning
    Article Snippet: .. Exogenous recombinant mouse BMP4 (mBMP4; R&D Systems 5020-BP) was used to overactivate the BMP pathway . ..

    Article Title: Cellular quiescence uncouples the proteome from the transcriptome in neural stem cells
    Article Snippet: .. NSCs were then propagated in Dulbecco’s modified Eagle’s medium (DMEM)/F-12 with Glutamax (Thermo Fischer Scientific 31331093) + N-2 MAX supplement (R&D Systems AR009) + penicillin–streptomycin (Thermo Fischer Scientific 15140) + 2 mg/ml laminin (Sigma L2020) + 5 mg/ml heparin (Sigma H3393-50KU) + 20 ng/ml recombinant murine fibroblast growth factor 2 (FGF-2) (PeproTech 450-33) + 20 ng/ml recombinant murine epidermal growth factor (EGF) (PeproTech 315-09) in tissue culture incubators at 37 °C with 5% CO 2 . qNSC induction was performed with 50 ng/ml BMP4 (R&D Systems 5020-BP-010) and qNSCs cultures were never passaged. .. Mouse cells were fixed in 4% paraformaldehyde (PFA) (Thermo Fisher Scientific J61899.AK) in PBS for 10 min at RT, washed in PBS, then stored 0.02% sodium azide in PBS at 4 °C.

    Sterility:

    Article Title: YAP regulates transcriptional programs for layer-specific periosteal expansion during fracture repair
    Article Snippet: As the proteins we were probing for were all in the range of 50 to 60 kDa, we used a light chain–specific, horseradish peroxidase–conjugated secondary antibody for detection (cat. no. 45262, Cell Signaling Technology). .. Recombinant mouse BMP4 (cat. no. 5020-BP, R&D Systems, Minneapolis, MN) was reconstituted in 0.9% sterile saline solution (cat. no. S5825, Teknova, Hollister, CA) at 12.5 μg/ml. ..

    Saline:

    Article Title: YAP regulates transcriptional programs for layer-specific periosteal expansion during fracture repair
    Article Snippet: As the proteins we were probing for were all in the range of 50 to 60 kDa, we used a light chain–specific, horseradish peroxidase–conjugated secondary antibody for detection (cat. no. 45262, Cell Signaling Technology). .. Recombinant mouse BMP4 (cat. no. 5020-BP, R&D Systems, Minneapolis, MN) was reconstituted in 0.9% sterile saline solution (cat. no. S5825, Teknova, Hollister, CA) at 12.5 μg/ml. ..

    Modification:

    Article Title: Cellular quiescence uncouples the proteome from the transcriptome in neural stem cells.
    Article Snippet: .. NSCs were then propagated in Dulbecco’s modified Eagle’s medium (DMEM)/F12 with Glutamax (Thermo Fischer Scientific 31331093)+N-2 MAX supplement (R&D Systems AR009) + penicillin–streptomycin (Thermo Fischer Scientific 15140)+ 2 mg/ml laminin (Sigma L2020)+ 5 mg/ml heparin (Sigma H3393-50KU)+ 20 ng/ml recombinant murine fibroblast growth factor 2 (FGF-2) (PeproTech 450-33)+ 20 ng/ml recombinant murine epidermal growth factor (EGF) (PeproTech 315-09) in tissue culture incubators at 37 °C with 5% CO2. qNSC induction was performed with 50 ng/ml BMP4 (R&D Systems 5020-BP-010) and qNSCs cultures were never passaged. .. Mouse cells were fixed in 4% paraformaldehyde (PFA) (Thermo Fisher Scientific J61899.AK) in PBS for 10 min at RT, washed in PBS, then stored 0.02% sodium azide in PBS at 4 °C.

    Article Title: Cellular quiescence uncouples the proteome from the transcriptome in neural stem cells
    Article Snippet: .. NSCs were then propagated in Dulbecco’s modified Eagle’s medium (DMEM)/F-12 with Glutamax (Thermo Fischer Scientific 31331093) + N-2 MAX supplement (R&D Systems AR009) + penicillin–streptomycin (Thermo Fischer Scientific 15140) + 2 mg/ml laminin (Sigma L2020) + 5 mg/ml heparin (Sigma H3393-50KU) + 20 ng/ml recombinant murine fibroblast growth factor 2 (FGF-2) (PeproTech 450-33) + 20 ng/ml recombinant murine epidermal growth factor (EGF) (PeproTech 315-09) in tissue culture incubators at 37 °C with 5% CO 2 . qNSC induction was performed with 50 ng/ml BMP4 (R&D Systems 5020-BP-010) and qNSCs cultures were never passaged. .. Mouse cells were fixed in 4% paraformaldehyde (PFA) (Thermo Fisher Scientific J61899.AK) in PBS for 10 min at RT, washed in PBS, then stored 0.02% sodium azide in PBS at 4 °C.

    Flow Cytometry:

    Article Title: Acute regulation of murine adipose tissue lipolysis and insulin resistance by the TGFβ superfamily protein GDF3
    Article Snippet: .. Recombinant proteins Recombinants Species Catalog No Company Used for GDF3 Mouse 9009-GD-010 R&D Systems Flow Cytometry, BLI, ITC, luciferase reporter assay, lipolysis GDF3 Human 5754-G3-010 R&D Systems BLI GDF3 Human 5754-G3-010/CF R&D Systems SPR BMP2 Human N/A Vicki Rosen lab Flow Cytometry, lipolysis BMP2 Human/ Mouse/ Rat 120-02C-10UG PeproTech ITC, lipolysis BMP4 Mouse 5020-BP-010 R&D Systems ITC, lipolysis BMP7 Mouse 5666-BP-010 R&D Systems Flow Cytometry BMP9 Human 3209-BP-010 R&D Systems Flow Cytometry, lipolysis BMP10 Mouse 6038-BP-025 R&D Systems Flow Cytometry, lipolysis TGFβ1 Human T7039 Millipore Sigma Flow Cytometry, lipolysis BMPRII-Fc Human/ Mouse 811-BR-100 R&D Systems SPR, BLI, ITC TGFBRII-Fc Human 341-BR-050/CF R&D Systems SPR ACTRIIA-Fc Human 340-R2-100/CF R&D Systems SPR ACTRIIB-Fc Human 339-RB-100/CF R&D Systems SPR IgG-Fc Human 160024 Gator Bio BLI IgG-Fc Mouse 160004 Gator Bio BLI Table S2. ..

    Luciferase:

    Article Title: Acute regulation of murine adipose tissue lipolysis and insulin resistance by the TGFβ superfamily protein GDF3
    Article Snippet: .. Recombinant proteins Recombinants Species Catalog No Company Used for GDF3 Mouse 9009-GD-010 R&D Systems Flow Cytometry, BLI, ITC, luciferase reporter assay, lipolysis GDF3 Human 5754-G3-010 R&D Systems BLI GDF3 Human 5754-G3-010/CF R&D Systems SPR BMP2 Human N/A Vicki Rosen lab Flow Cytometry, lipolysis BMP2 Human/ Mouse/ Rat 120-02C-10UG PeproTech ITC, lipolysis BMP4 Mouse 5020-BP-010 R&D Systems ITC, lipolysis BMP7 Mouse 5666-BP-010 R&D Systems Flow Cytometry BMP9 Human 3209-BP-010 R&D Systems Flow Cytometry, lipolysis BMP10 Mouse 6038-BP-025 R&D Systems Flow Cytometry, lipolysis TGFβ1 Human T7039 Millipore Sigma Flow Cytometry, lipolysis BMPRII-Fc Human/ Mouse 811-BR-100 R&D Systems SPR, BLI, ITC TGFBRII-Fc Human 341-BR-050/CF R&D Systems SPR ACTRIIA-Fc Human 340-R2-100/CF R&D Systems SPR ACTRIIB-Fc Human 339-RB-100/CF R&D Systems SPR IgG-Fc Human 160024 Gator Bio BLI IgG-Fc Mouse 160004 Gator Bio BLI Table S2. ..

    Reporter Assay:

    Article Title: Acute regulation of murine adipose tissue lipolysis and insulin resistance by the TGFβ superfamily protein GDF3
    Article Snippet: .. Recombinant proteins Recombinants Species Catalog No Company Used for GDF3 Mouse 9009-GD-010 R&D Systems Flow Cytometry, BLI, ITC, luciferase reporter assay, lipolysis GDF3 Human 5754-G3-010 R&D Systems BLI GDF3 Human 5754-G3-010/CF R&D Systems SPR BMP2 Human N/A Vicki Rosen lab Flow Cytometry, lipolysis BMP2 Human/ Mouse/ Rat 120-02C-10UG PeproTech ITC, lipolysis BMP4 Mouse 5020-BP-010 R&D Systems ITC, lipolysis BMP7 Mouse 5666-BP-010 R&D Systems Flow Cytometry BMP9 Human 3209-BP-010 R&D Systems Flow Cytometry, lipolysis BMP10 Mouse 6038-BP-025 R&D Systems Flow Cytometry, lipolysis TGFβ1 Human T7039 Millipore Sigma Flow Cytometry, lipolysis BMPRII-Fc Human/ Mouse 811-BR-100 R&D Systems SPR, BLI, ITC TGFBRII-Fc Human 341-BR-050/CF R&D Systems SPR ACTRIIA-Fc Human 340-R2-100/CF R&D Systems SPR ACTRIIB-Fc Human 339-RB-100/CF R&D Systems SPR IgG-Fc Human 160024 Gator Bio BLI IgG-Fc Mouse 160004 Gator Bio BLI Table S2. ..

    SPR Assay:

    Article Title: Acute regulation of murine adipose tissue lipolysis and insulin resistance by the TGFβ superfamily protein GDF3
    Article Snippet: .. Recombinant proteins Recombinants Species Catalog No Company Used for GDF3 Mouse 9009-GD-010 R&D Systems Flow Cytometry, BLI, ITC, luciferase reporter assay, lipolysis GDF3 Human 5754-G3-010 R&D Systems BLI GDF3 Human 5754-G3-010/CF R&D Systems SPR BMP2 Human N/A Vicki Rosen lab Flow Cytometry, lipolysis BMP2 Human/ Mouse/ Rat 120-02C-10UG PeproTech ITC, lipolysis BMP4 Mouse 5020-BP-010 R&D Systems ITC, lipolysis BMP7 Mouse 5666-BP-010 R&D Systems Flow Cytometry BMP9 Human 3209-BP-010 R&D Systems Flow Cytometry, lipolysis BMP10 Mouse 6038-BP-025 R&D Systems Flow Cytometry, lipolysis TGFβ1 Human T7039 Millipore Sigma Flow Cytometry, lipolysis BMPRII-Fc Human/ Mouse 811-BR-100 R&D Systems SPR, BLI, ITC TGFBRII-Fc Human 341-BR-050/CF R&D Systems SPR ACTRIIA-Fc Human 340-R2-100/CF R&D Systems SPR ACTRIIB-Fc Human 339-RB-100/CF R&D Systems SPR IgG-Fc Human 160024 Gator Bio BLI IgG-Fc Mouse 160004 Gator Bio BLI Table S2. ..



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    Image Search Results


    Signaling factors required for generation of human ARC in vitro (A) Hypothalamic development in vivo and in vitro differentiation of hPSC into hypothalamic cells. (B) Principal-component analysis (PCA) of day 16 RT-qPCR data (RC17), clustering according to ARC- and PVN-associated genes. Each dot represents one differentiation batch ( n = 113). (C) Estimated marginal means assessing the effect of factors on PC1, adjusted for covariates. (D) ICC/ISH at days 16 and 50 for ARC protocol with or without BMP4. Scale bars: 100 μm. (E) ARC differentiation protocol and maturation options. (F–H) ICC of day 50 in 2D (F) and 3D (G and H), scale bars: 50 and 100 μm. (I) Quantification of day 50 3D spheroids (AGRP: n = 14, 4.53% ± 0.69%, mean ± SEM; αMSH: n = 6, 5.90% ± 1.19%). (J) Xenotransplantation of day 16 ARC progenitors into nude rats. (J′) Top: ICC for human neural cell adhesion molecule (NCAM) highlights graft site, scale bar: 2 mm. Bottom: IF for human nuclear antigen (HuNu), scale bar: 200 μm. (K–Q) IF for ARC neuronal markers in grafts. Scale bars: 100 μm (K and O), 50 μm (N), 25 μm (L, M, P, and Q).

    Journal: Cell Stem Cell

    Article Title: Generation of human appetite-regulating neurons and tanycytes from pluripotent stem cells

    doi: 10.1016/j.stem.2026.05.005

    Figure Lengend Snippet: Signaling factors required for generation of human ARC in vitro (A) Hypothalamic development in vivo and in vitro differentiation of hPSC into hypothalamic cells. (B) Principal-component analysis (PCA) of day 16 RT-qPCR data (RC17), clustering according to ARC- and PVN-associated genes. Each dot represents one differentiation batch ( n = 113). (C) Estimated marginal means assessing the effect of factors on PC1, adjusted for covariates. (D) ICC/ISH at days 16 and 50 for ARC protocol with or without BMP4. Scale bars: 100 μm. (E) ARC differentiation protocol and maturation options. (F–H) ICC of day 50 in 2D (F) and 3D (G and H), scale bars: 50 and 100 μm. (I) Quantification of day 50 3D spheroids (AGRP: n = 14, 4.53% ± 0.69%, mean ± SEM; αMSH: n = 6, 5.90% ± 1.19%). (J) Xenotransplantation of day 16 ARC progenitors into nude rats. (J′) Top: ICC for human neural cell adhesion molecule (NCAM) highlights graft site, scale bar: 2 mm. Bottom: IF for human nuclear antigen (HuNu), scale bar: 200 μm. (K–Q) IF for ARC neuronal markers in grafts. Scale bars: 100 μm (K and O), 50 μm (N), 25 μm (L, M, P, and Q).

    Article Snippet: BMP4 , Miltenyi Biotec , Cat#130-111-168.

    Techniques: In Vitro, In Vivo, Quantitative RT-PCR

    Duration of BMP stimulation controls ARC versus VMH identity (A) BMP signaling in the developing chick tuberal hypothalamus. Adapted from Chinnaiya et al. vFB, ventral forebrain. (B) Experimental design to simulate BMP withdrawal. (C) ICC/ISH for days 16 and 50 across BMP4 withdrawal conditions. Scale bar: 100 μm. (D) Day 16 RT-qPCR (RC17, KOLF2.1J, BIONi010) across BMP4 withdrawal conditions. Mean ± SEM, Kruskal-Wallis with Dunn’s multiple comparisons test: FGF10 : −BMP versus days 5–14 p < 0.0001; days 5–7 versus days 5–14 p < 0.0001; days 5–9 versus days 5–14 p = 0.0040; days 5–11 versus days 5–14 p = 0.013; SOX14 : −BMP versus days 5–14 p < 0.0001; days 5–7 versus days 5–14 p = 0.0004; days 5–9 versus days 5–14 p = 0.0011; days 5–11 versus days 5–14 p = 0.0020. (E) Day 50 RT-qPCR (RC17, KOLF2.1J, BIONi010) across BMP4 withdrawal conditions. Mean ± SEM, Kruskal-Wallis with Dunn’s test: AGRP : −BMP versus days 5–14 p = 0.0080; days 5–7 versus days 5–14 p = 0.0269 NR5A1 : −BMP versus days 5–14 p = 0.0016; days 5–7 versus days 5–14 p = 0.0162. (F and G) UMAPs of day 16 (F) and days 50 + 80 + 110 (G) snRNA-seq ( n = 3; RC17, KOLF2.1J, BIONi010) and annotated clusters with proportional representation. ARC- and VMH-specific clusters are marked with a box. (H) Spatial projection probability of days 50 + 80 + 110 clusters onto human adult reference. (I) 3D staining for Wisteria floribunda agglutinin (WFA), scale bar: 100 μm.

    Journal: Cell Stem Cell

    Article Title: Generation of human appetite-regulating neurons and tanycytes from pluripotent stem cells

    doi: 10.1016/j.stem.2026.05.005

    Figure Lengend Snippet: Duration of BMP stimulation controls ARC versus VMH identity (A) BMP signaling in the developing chick tuberal hypothalamus. Adapted from Chinnaiya et al. vFB, ventral forebrain. (B) Experimental design to simulate BMP withdrawal. (C) ICC/ISH for days 16 and 50 across BMP4 withdrawal conditions. Scale bar: 100 μm. (D) Day 16 RT-qPCR (RC17, KOLF2.1J, BIONi010) across BMP4 withdrawal conditions. Mean ± SEM, Kruskal-Wallis with Dunn’s multiple comparisons test: FGF10 : −BMP versus days 5–14 p < 0.0001; days 5–7 versus days 5–14 p < 0.0001; days 5–9 versus days 5–14 p = 0.0040; days 5–11 versus days 5–14 p = 0.013; SOX14 : −BMP versus days 5–14 p < 0.0001; days 5–7 versus days 5–14 p = 0.0004; days 5–9 versus days 5–14 p = 0.0011; days 5–11 versus days 5–14 p = 0.0020. (E) Day 50 RT-qPCR (RC17, KOLF2.1J, BIONi010) across BMP4 withdrawal conditions. Mean ± SEM, Kruskal-Wallis with Dunn’s test: AGRP : −BMP versus days 5–14 p = 0.0080; days 5–7 versus days 5–14 p = 0.0269 NR5A1 : −BMP versus days 5–14 p = 0.0016; days 5–7 versus days 5–14 p = 0.0162. (F and G) UMAPs of day 16 (F) and days 50 + 80 + 110 (G) snRNA-seq ( n = 3; RC17, KOLF2.1J, BIONi010) and annotated clusters with proportional representation. ARC- and VMH-specific clusters are marked with a box. (H) Spatial projection probability of days 50 + 80 + 110 clusters onto human adult reference. (I) 3D staining for Wisteria floribunda agglutinin (WFA), scale bar: 100 μm.

    Article Snippet: BMP4 , Miltenyi Biotec , Cat#130-111-168.

    Techniques: Quantitative RT-PCR, Staining

    Different POMC subtypes are dependent on BMP timing (A) UMAP of days 50 + 80 + 110 snRNA-seq data from G with POMC clusters and their % across BMP4 exposure timings. (B) Key marker genes across POMC + ARC and VMH clusters in in vitro dataset and human fetal and adult reference datasets. , , (C) MetaNeighbor cluster similarity analysis comparing in vitro POMC⁺ clusters to hypothalamic reference datasets. , , (D) Expression of ARC/VMH-related markers on spatial transcriptomics reference. (E) DEG between in vitro POMC⁺ subtypes, spatial DEGs from HYPOMAP are highlighted. Benjamini-Hochberg corrected p values. (F) Prediction probabilities from spatial mapping of in vitro POMC clusters to HYPOMAP. (G) Hybridization chain reaction (HCR) of chick embryo heads at HH17 and HH20, n = 7–10. Scale bar: 100 μm.

    Journal: Cell Stem Cell

    Article Title: Generation of human appetite-regulating neurons and tanycytes from pluripotent stem cells

    doi: 10.1016/j.stem.2026.05.005

    Figure Lengend Snippet: Different POMC subtypes are dependent on BMP timing (A) UMAP of days 50 + 80 + 110 snRNA-seq data from G with POMC clusters and their % across BMP4 exposure timings. (B) Key marker genes across POMC + ARC and VMH clusters in in vitro dataset and human fetal and adult reference datasets. , , (C) MetaNeighbor cluster similarity analysis comparing in vitro POMC⁺ clusters to hypothalamic reference datasets. , , (D) Expression of ARC/VMH-related markers on spatial transcriptomics reference. (E) DEG between in vitro POMC⁺ subtypes, spatial DEGs from HYPOMAP are highlighted. Benjamini-Hochberg corrected p values. (F) Prediction probabilities from spatial mapping of in vitro POMC clusters to HYPOMAP. (G) Hybridization chain reaction (HCR) of chick embryo heads at HH17 and HH20, n = 7–10. Scale bar: 100 μm.

    Article Snippet: BMP4 , Miltenyi Biotec , Cat#130-111-168.

    Techniques: Marker, In Vitro, Expressing, Spatial Transcriptomics, Hybridization