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bone morphogenetic protein 4  (MedChemExpress)


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    MedChemExpress bone morphogenetic protein 4
    Bone Morphogenetic Protein 4, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 8 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bmp-4/BMP-4%2C+Human/pm42224858-40-9-42
    Average 95 stars, based on 8 article reviews
    bone morphogenetic protein 4 - by Bioz Stars, 2026-09
    95/100 stars

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    Recombinant:

    Article Title: POSTN + fibroblast-secreted small extracellular vesicles drive macrophage M2 polarization through BMP4/BMPR2/Smad signaling
    Article Snippet: The human monocytic leukemia cell line THP-1 was grown in a T25 culture flask supplemented with RPMI 1640 medium(Gibco; Thermo Fisher Scientific, Inc.) containing 50 ng/ml of phorbol 12-myristate 13-acetate (PMA; cat. no. P8139; Sigma-Aldrich; Merck KGaA) for 48 h. Then, they were maintained in fresh RPMI 1640 culture medium for 24 h to stabilize their growth. .. To confirm that BMP4 promotes macrophage polarization toward the M2 phenotype, THP-1 (2×10 6 cells) were seeded in 6-well plates and differentiated into macrophages using 50 ng/ml PMA for 48 h. After stabilization, macrophages were assigned to different groups and then either left unstimulated or stimulated for an additional 48 h with recombinant human BMP4 (50 or 100 ng/ml; cat. no. HY-P7007; MedChemExpress). .. Macrophages were stained with FITC anti-human CD163 antibody (cat. no. 333617; 1:50; Biolegend Inc.) and FITC anti-human CD206 antibody (cat. no. 321103; 1:50; Biolegend Inc.) at 4°C for 15 min and then centrifugation 1,000 × g at 4°C for 5 min. Pellets were resuspended in 1 ml PBS and analyzed the expression of M2 macrophage polarization biomarkers (CD163 and CD206) was then analyzed by Flow cytometry (NovoCyte 2040R; ACEA Biosciences) equipped with NovoExpress software (version 1.6.3; http://explore.agilent.com/ACEA-joins-Agilent ).

    Article Title: A CYP3A7 metabolically competent human induced pluripotent stem cell-derived hepatocyte-like cell (hHLC 3A7 ) model for drug safety assessment in the newborn.
    Article Snippet: Drug safety assessment in newborns is limited by ethical constraints and the fact that no appropriate human-relevant in vitro hepatic cell model system currently exists to study and predict drug metabolism and disposition in this fragile population.. Yet polypharmacy and off-label drug administration to newborns in the neonatal intensive care unit substantially increases the risk for drug adverse events in a population characterized by an immature hepatic drug metabolizing enzyme (DME) system.. To address this concern, we differentiated induced pluripotent stem cells from adult donors into human hepatocyte-like cells (hHLC3A7) exhibiting metabolic activity of the unique fetal/newborn CYP3A7.



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    Signaling factors required for generation of human ARC in vitro (A) Hypothalamic development in vivo and in vitro differentiation of hPSC into hypothalamic cells. (B) Principal-component analysis (PCA) of day 16 RT-qPCR data (RC17), clustering according to ARC- and PVN-associated genes. Each dot represents one differentiation batch ( n = 113). (C) Estimated marginal means assessing the effect of factors on PC1, adjusted for covariates. (D) ICC/ISH at days 16 and 50 for ARC protocol with or without <t>BMP4.</t> Scale bars: 100 μm. (E) ARC differentiation protocol and maturation options. (F–H) ICC of day 50 in 2D (F) and 3D (G and H), scale bars: 50 and 100 μm. (I) Quantification of day 50 3D spheroids (AGRP: n = 14, 4.53% ± 0.69%, mean ± SEM; αMSH: n = 6, 5.90% ± 1.19%). (J) Xenotransplantation of day 16 ARC progenitors into nude rats. (J′) Top: ICC for human neural cell adhesion molecule (NCAM) highlights graft site, scale bar: 2 mm. Bottom: IF for human nuclear antigen (HuNu), scale bar: 200 μm. (K–Q) IF for ARC neuronal markers in grafts. Scale bars: 100 μm (K and O), 50 μm (N), 25 μm (L, M, P, and Q).
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    Signaling factors required for generation of human ARC in vitro (A) Hypothalamic development in vivo and in vitro differentiation of hPSC into hypothalamic cells. (B) Principal-component analysis (PCA) of day 16 RT-qPCR data (RC17), clustering according to ARC- and PVN-associated genes. Each dot represents one differentiation batch ( n = 113). (C) Estimated marginal means assessing the effect of factors on PC1, adjusted for covariates. (D) ICC/ISH at days 16 and 50 for ARC protocol with or without <t>BMP4.</t> Scale bars: 100 μm. (E) ARC differentiation protocol and maturation options. (F–H) ICC of day 50 in 2D (F) and 3D (G and H), scale bars: 50 and 100 μm. (I) Quantification of day 50 3D spheroids (AGRP: n = 14, 4.53% ± 0.69%, mean ± SEM; αMSH: n = 6, 5.90% ± 1.19%). (J) Xenotransplantation of day 16 ARC progenitors into nude rats. (J′) Top: ICC for human neural cell adhesion molecule (NCAM) highlights graft site, scale bar: 2 mm. Bottom: IF for human nuclear antigen (HuNu), scale bar: 200 μm. (K–Q) IF for ARC neuronal markers in grafts. Scale bars: 100 μm (K and O), 50 μm (N), 25 μm (L, M, P, and Q).
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    Signaling factors required for generation of human ARC in vitro (A) Hypothalamic development in vivo and in vitro differentiation of hPSC into hypothalamic cells. (B) Principal-component analysis (PCA) of day 16 RT-qPCR data (RC17), clustering according to ARC- and PVN-associated genes. Each dot represents one differentiation batch ( n = 113). (C) Estimated marginal means assessing the effect of factors on PC1, adjusted for covariates. (D) ICC/ISH at days 16 and 50 for ARC protocol with or without <t>BMP4.</t> Scale bars: 100 μm. (E) ARC differentiation protocol and maturation options. (F–H) ICC of day 50 in 2D (F) and 3D (G and H), scale bars: 50 and 100 μm. (I) Quantification of day 50 3D spheroids (AGRP: n = 14, 4.53% ± 0.69%, mean ± SEM; αMSH: n = 6, 5.90% ± 1.19%). (J) Xenotransplantation of day 16 ARC progenitors into nude rats. (J′) Top: ICC for human neural cell adhesion molecule (NCAM) highlights graft site, scale bar: 2 mm. Bottom: IF for human nuclear antigen (HuNu), scale bar: 200 μm. (K–Q) IF for ARC neuronal markers in grafts. Scale bars: 100 μm (K and O), 50 μm (N), 25 μm (L, M, P, and Q).
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    Signaling factors required for generation of human ARC in vitro (A) Hypothalamic development in vivo and in vitro differentiation of hPSC into hypothalamic cells. (B) Principal-component analysis (PCA) of day 16 RT-qPCR data (RC17), clustering according to ARC- and PVN-associated genes. Each dot represents one differentiation batch ( n = 113). (C) Estimated marginal means assessing the effect of factors on PC1, adjusted for covariates. (D) ICC/ISH at days 16 and 50 for ARC protocol with or without <t>BMP4.</t> Scale bars: 100 μm. (E) ARC differentiation protocol and maturation options. (F–H) ICC of day 50 in 2D (F) and 3D (G and H), scale bars: 50 and 100 μm. (I) Quantification of day 50 3D spheroids (AGRP: n = 14, 4.53% ± 0.69%, mean ± SEM; αMSH: n = 6, 5.90% ± 1.19%). (J) Xenotransplantation of day 16 ARC progenitors into nude rats. (J′) Top: ICC for human neural cell adhesion molecule (NCAM) highlights graft site, scale bar: 2 mm. Bottom: IF for human nuclear antigen (HuNu), scale bar: 200 μm. (K–Q) IF for ARC neuronal markers in grafts. Scale bars: 100 μm (K and O), 50 μm (N), 25 μm (L, M, P, and Q).
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    Signaling factors required for generation of human ARC in vitro (A) Hypothalamic development in vivo and in vitro differentiation of hPSC into hypothalamic cells. (B) Principal-component analysis (PCA) of day 16 RT-qPCR data (RC17), clustering according to ARC- and PVN-associated genes. Each dot represents one differentiation batch ( n = 113). (C) Estimated marginal means assessing the effect of factors on PC1, adjusted for covariates. (D) ICC/ISH at days 16 and 50 for ARC protocol with or without <t>BMP4.</t> Scale bars: 100 μm. (E) ARC differentiation protocol and maturation options. (F–H) ICC of day 50 in 2D (F) and 3D (G and H), scale bars: 50 and 100 μm. (I) Quantification of day 50 3D spheroids (AGRP: n = 14, 4.53% ± 0.69%, mean ± SEM; αMSH: n = 6, 5.90% ± 1.19%). (J) Xenotransplantation of day 16 ARC progenitors into nude rats. (J′) Top: ICC for human neural cell adhesion molecule (NCAM) highlights graft site, scale bar: 2 mm. Bottom: IF for human nuclear antigen (HuNu), scale bar: 200 μm. (K–Q) IF for ARC neuronal markers in grafts. Scale bars: 100 μm (K and O), 50 μm (N), 25 μm (L, M, P, and Q).
    Lpc Medium, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    bone morphogenetic protein 4  (MedChemExpress)
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    Signaling factors required for generation of human ARC in vitro (A) Hypothalamic development in vivo and in vitro differentiation of hPSC into hypothalamic cells. (B) Principal-component analysis (PCA) of day 16 RT-qPCR data (RC17), clustering according to ARC- and PVN-associated genes. Each dot represents one differentiation batch ( n = 113). (C) Estimated marginal means assessing the effect of factors on PC1, adjusted for covariates. (D) ICC/ISH at days 16 and 50 for ARC protocol with or without <t>BMP4.</t> Scale bars: 100 μm. (E) ARC differentiation protocol and maturation options. (F–H) ICC of day 50 in 2D (F) and 3D (G and H), scale bars: 50 and 100 μm. (I) Quantification of day 50 3D spheroids (AGRP: n = 14, 4.53% ± 0.69%, mean ± SEM; αMSH: n = 6, 5.90% ± 1.19%). (J) Xenotransplantation of day 16 ARC progenitors into nude rats. (J′) Top: ICC for human neural cell adhesion molecule (NCAM) highlights graft site, scale bar: 2 mm. Bottom: IF for human nuclear antigen (HuNu), scale bar: 200 μm. (K–Q) IF for ARC neuronal markers in grafts. Scale bars: 100 μm (K and O), 50 μm (N), 25 μm (L, M, P, and Q).
    Bone Morphogenetic Protein 4, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Signaling factors required for generation of human ARC in vitro (A) Hypothalamic development in vivo and in vitro differentiation of hPSC into hypothalamic cells. (B) Principal-component analysis (PCA) of day 16 RT-qPCR data (RC17), clustering according to ARC- and PVN-associated genes. Each dot represents one differentiation batch ( n = 113). (C) Estimated marginal means assessing the effect of factors on PC1, adjusted for covariates. (D) ICC/ISH at days 16 and 50 for ARC protocol with or without BMP4. Scale bars: 100 μm. (E) ARC differentiation protocol and maturation options. (F–H) ICC of day 50 in 2D (F) and 3D (G and H), scale bars: 50 and 100 μm. (I) Quantification of day 50 3D spheroids (AGRP: n = 14, 4.53% ± 0.69%, mean ± SEM; αMSH: n = 6, 5.90% ± 1.19%). (J) Xenotransplantation of day 16 ARC progenitors into nude rats. (J′) Top: ICC for human neural cell adhesion molecule (NCAM) highlights graft site, scale bar: 2 mm. Bottom: IF for human nuclear antigen (HuNu), scale bar: 200 μm. (K–Q) IF for ARC neuronal markers in grafts. Scale bars: 100 μm (K and O), 50 μm (N), 25 μm (L, M, P, and Q).

    Journal: Cell Stem Cell

    Article Title: Generation of human appetite-regulating neurons and tanycytes from pluripotent stem cells

    doi: 10.1016/j.stem.2026.05.005

    Figure Lengend Snippet: Signaling factors required for generation of human ARC in vitro (A) Hypothalamic development in vivo and in vitro differentiation of hPSC into hypothalamic cells. (B) Principal-component analysis (PCA) of day 16 RT-qPCR data (RC17), clustering according to ARC- and PVN-associated genes. Each dot represents one differentiation batch ( n = 113). (C) Estimated marginal means assessing the effect of factors on PC1, adjusted for covariates. (D) ICC/ISH at days 16 and 50 for ARC protocol with or without BMP4. Scale bars: 100 μm. (E) ARC differentiation protocol and maturation options. (F–H) ICC of day 50 in 2D (F) and 3D (G and H), scale bars: 50 and 100 μm. (I) Quantification of day 50 3D spheroids (AGRP: n = 14, 4.53% ± 0.69%, mean ± SEM; αMSH: n = 6, 5.90% ± 1.19%). (J) Xenotransplantation of day 16 ARC progenitors into nude rats. (J′) Top: ICC for human neural cell adhesion molecule (NCAM) highlights graft site, scale bar: 2 mm. Bottom: IF for human nuclear antigen (HuNu), scale bar: 200 μm. (K–Q) IF for ARC neuronal markers in grafts. Scale bars: 100 μm (K and O), 50 μm (N), 25 μm (L, M, P, and Q).

    Article Snippet: BMP4 , Miltenyi Biotec , Cat#130-111-168.

    Techniques: In Vitro, In Vivo, Quantitative RT-PCR

    Duration of BMP stimulation controls ARC versus VMH identity (A) BMP signaling in the developing chick tuberal hypothalamus. Adapted from Chinnaiya et al. vFB, ventral forebrain. (B) Experimental design to simulate BMP withdrawal. (C) ICC/ISH for days 16 and 50 across BMP4 withdrawal conditions. Scale bar: 100 μm. (D) Day 16 RT-qPCR (RC17, KOLF2.1J, BIONi010) across BMP4 withdrawal conditions. Mean ± SEM, Kruskal-Wallis with Dunn’s multiple comparisons test: FGF10 : −BMP versus days 5–14 p < 0.0001; days 5–7 versus days 5–14 p < 0.0001; days 5–9 versus days 5–14 p = 0.0040; days 5–11 versus days 5–14 p = 0.013; SOX14 : −BMP versus days 5–14 p < 0.0001; days 5–7 versus days 5–14 p = 0.0004; days 5–9 versus days 5–14 p = 0.0011; days 5–11 versus days 5–14 p = 0.0020. (E) Day 50 RT-qPCR (RC17, KOLF2.1J, BIONi010) across BMP4 withdrawal conditions. Mean ± SEM, Kruskal-Wallis with Dunn’s test: AGRP : −BMP versus days 5–14 p = 0.0080; days 5–7 versus days 5–14 p = 0.0269 NR5A1 : −BMP versus days 5–14 p = 0.0016; days 5–7 versus days 5–14 p = 0.0162. (F and G) UMAPs of day 16 (F) and days 50 + 80 + 110 (G) snRNA-seq ( n = 3; RC17, KOLF2.1J, BIONi010) and annotated clusters with proportional representation. ARC- and VMH-specific clusters are marked with a box. (H) Spatial projection probability of days 50 + 80 + 110 clusters onto human adult reference. (I) 3D staining for Wisteria floribunda agglutinin (WFA), scale bar: 100 μm.

    Journal: Cell Stem Cell

    Article Title: Generation of human appetite-regulating neurons and tanycytes from pluripotent stem cells

    doi: 10.1016/j.stem.2026.05.005

    Figure Lengend Snippet: Duration of BMP stimulation controls ARC versus VMH identity (A) BMP signaling in the developing chick tuberal hypothalamus. Adapted from Chinnaiya et al. vFB, ventral forebrain. (B) Experimental design to simulate BMP withdrawal. (C) ICC/ISH for days 16 and 50 across BMP4 withdrawal conditions. Scale bar: 100 μm. (D) Day 16 RT-qPCR (RC17, KOLF2.1J, BIONi010) across BMP4 withdrawal conditions. Mean ± SEM, Kruskal-Wallis with Dunn’s multiple comparisons test: FGF10 : −BMP versus days 5–14 p < 0.0001; days 5–7 versus days 5–14 p < 0.0001; days 5–9 versus days 5–14 p = 0.0040; days 5–11 versus days 5–14 p = 0.013; SOX14 : −BMP versus days 5–14 p < 0.0001; days 5–7 versus days 5–14 p = 0.0004; days 5–9 versus days 5–14 p = 0.0011; days 5–11 versus days 5–14 p = 0.0020. (E) Day 50 RT-qPCR (RC17, KOLF2.1J, BIONi010) across BMP4 withdrawal conditions. Mean ± SEM, Kruskal-Wallis with Dunn’s test: AGRP : −BMP versus days 5–14 p = 0.0080; days 5–7 versus days 5–14 p = 0.0269 NR5A1 : −BMP versus days 5–14 p = 0.0016; days 5–7 versus days 5–14 p = 0.0162. (F and G) UMAPs of day 16 (F) and days 50 + 80 + 110 (G) snRNA-seq ( n = 3; RC17, KOLF2.1J, BIONi010) and annotated clusters with proportional representation. ARC- and VMH-specific clusters are marked with a box. (H) Spatial projection probability of days 50 + 80 + 110 clusters onto human adult reference. (I) 3D staining for Wisteria floribunda agglutinin (WFA), scale bar: 100 μm.

    Article Snippet: BMP4 , Miltenyi Biotec , Cat#130-111-168.

    Techniques: Quantitative RT-PCR, Staining

    Different POMC subtypes are dependent on BMP timing (A) UMAP of days 50 + 80 + 110 snRNA-seq data from G with POMC clusters and their % across BMP4 exposure timings. (B) Key marker genes across POMC + ARC and VMH clusters in in vitro dataset and human fetal and adult reference datasets. , , (C) MetaNeighbor cluster similarity analysis comparing in vitro POMC⁺ clusters to hypothalamic reference datasets. , , (D) Expression of ARC/VMH-related markers on spatial transcriptomics reference. (E) DEG between in vitro POMC⁺ subtypes, spatial DEGs from HYPOMAP are highlighted. Benjamini-Hochberg corrected p values. (F) Prediction probabilities from spatial mapping of in vitro POMC clusters to HYPOMAP. (G) Hybridization chain reaction (HCR) of chick embryo heads at HH17 and HH20, n = 7–10. Scale bar: 100 μm.

    Journal: Cell Stem Cell

    Article Title: Generation of human appetite-regulating neurons and tanycytes from pluripotent stem cells

    doi: 10.1016/j.stem.2026.05.005

    Figure Lengend Snippet: Different POMC subtypes are dependent on BMP timing (A) UMAP of days 50 + 80 + 110 snRNA-seq data from G with POMC clusters and their % across BMP4 exposure timings. (B) Key marker genes across POMC + ARC and VMH clusters in in vitro dataset and human fetal and adult reference datasets. , , (C) MetaNeighbor cluster similarity analysis comparing in vitro POMC⁺ clusters to hypothalamic reference datasets. , , (D) Expression of ARC/VMH-related markers on spatial transcriptomics reference. (E) DEG between in vitro POMC⁺ subtypes, spatial DEGs from HYPOMAP are highlighted. Benjamini-Hochberg corrected p values. (F) Prediction probabilities from spatial mapping of in vitro POMC clusters to HYPOMAP. (G) Hybridization chain reaction (HCR) of chick embryo heads at HH17 and HH20, n = 7–10. Scale bar: 100 μm.

    Article Snippet: BMP4 , Miltenyi Biotec , Cat#130-111-168.

    Techniques: Marker, In Vitro, Expressing, Spatial Transcriptomics, Hybridization