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primary rat bone marrow derived macrophages bmdms  (Procell Inc)

 
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    Procell Inc primary rat bone marrow derived macrophages bmdms
    Primary Rat Bone Marrow Derived Macrophages Bmdms, supplied by Procell Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bmdms/bone+cells+marrow+mesenchymal+rat+stem/pmc12964221-259-0-15
    Average 86 stars, based on 1 article reviews
    primary rat bone marrow derived macrophages bmdms - by Bioz Stars, 2026-10
    86/100 stars

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    Cell Culture:

    Article Title: Topical framework nucleic acid transdermal delivery system for reprogramming cutaneous dendritic cells to maintain graft immune homeostasis
    Article Snippet: .. The mouse bone marrow–derived dendritic cell line DC2.4 was purchased from Procell Life Science & Technology Co., Ltd. (Wuhan, China) and cultured in a specialized medium (CM-0545), comprising RPMI-1640 (PM150110) supplemented with 10% fetal bovine serum (FBS, 164210-50) and 1% penicillin–streptomycin (P/S, PB180120 ), in a humidified incubator at 37 °C under 5% CO 2 atmosphere. ..

    Article Title: Potentials of BMSCs for regulating osteogenic-vascular-neural-lymphatic coupling in bone regeneration.
    Article Snippet: .. Human bone mesenchymal stem cells (hBMSCs) were purchased from Procell Life Science & Technology Co. Ltd and cultured in Minimum Essential Medium α (MEM α) (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 1% streptomycin and penicillin (Solarbio, Beijing, China) and 10% fetal bovine serum (Gibco, Thermo Fisher Scientific, Waltham, MA, USA) at 37 °C in a 5% CO2 atmosphere. ..



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    Co-culture of BMDMs and MEFs in stiff 3D hydrogels increases CD54 and CD140a expression on macrophages. BMDMs and MEFs were co-cultured in 3D collagen-vitronectin, collagen-vitronectin-5% alginate scaffolds, or 2D controls for 24 hours. The viability, proportion of each cell type and surface expression of key markers was determined by flow cytometry. (A) Representative gating strategy for the separation and analysis of co-cultured BMDMs and MEFs by flow cytometry. Sample shown are BMDMs and MEFs cultured in stiff collagen-vitronectin-5% alginate hydrogels. (B) Overall cell viability. Note that this represents a mixture of BMDMs and MEFs. (C) Proportion of BMDMs (CD45 + CD11b + F4/80 + ) and MEFs (CD45 - CD11b - F4/80 - ) in the indicated co-culture conditions. (D, E) Geometric mean fluorescence intensity (gMFI) of CD11b and F4/80 expression on co-cultured BMDMs. (F) Representative histograms for CD54 and CD140a expression on BMDMs (left) and MEFs (right). (G–J) The relative expression of CD54 (G, H) and CD140a (I, J) on BMDMs and MEFs after co-culture (G, I) or single-culture (H–J) in the indicated models. Some single-cultured BMDMs were also activated with LPS and IFN-γ or IL-4 and IL-13, as indicated. Data in G-J are the relative gMFI, calculated by dividing gMFI values by the mean gMFI of unactivated BMDMs cultured in 2D. B-E and G-J show the pooled means of 3–4 independent experiments. Errors are SEM. Groups in B, D, E, H and J were tested by one-way ANOVA; C, G and I by two-way ANOVA where for the latter overall ANOVA results are indicated above each graph. Post-hoc analyses were performed for one- and two-way ANOVAs where significance between groups is indicated as **p<0.01, ***p<0.001, ****p<0.0001, ns, not significant.

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    Figure Lengend Snippet: Co-culture of BMDMs and MEFs in stiff 3D hydrogels increases CD54 and CD140a expression on macrophages. BMDMs and MEFs were co-cultured in 3D collagen-vitronectin, collagen-vitronectin-5% alginate scaffolds, or 2D controls for 24 hours. The viability, proportion of each cell type and surface expression of key markers was determined by flow cytometry. (A) Representative gating strategy for the separation and analysis of co-cultured BMDMs and MEFs by flow cytometry. Sample shown are BMDMs and MEFs cultured in stiff collagen-vitronectin-5% alginate hydrogels. (B) Overall cell viability. Note that this represents a mixture of BMDMs and MEFs. (C) Proportion of BMDMs (CD45 + CD11b + F4/80 + ) and MEFs (CD45 - CD11b - F4/80 - ) in the indicated co-culture conditions. (D, E) Geometric mean fluorescence intensity (gMFI) of CD11b and F4/80 expression on co-cultured BMDMs. (F) Representative histograms for CD54 and CD140a expression on BMDMs (left) and MEFs (right). (G–J) The relative expression of CD54 (G, H) and CD140a (I, J) on BMDMs and MEFs after co-culture (G, I) or single-culture (H–J) in the indicated models. Some single-cultured BMDMs were also activated with LPS and IFN-γ or IL-4 and IL-13, as indicated. Data in G-J are the relative gMFI, calculated by dividing gMFI values by the mean gMFI of unactivated BMDMs cultured in 2D. B-E and G-J show the pooled means of 3–4 independent experiments. Errors are SEM. Groups in B, D, E, H and J were tested by one-way ANOVA; C, G and I by two-way ANOVA where for the latter overall ANOVA results are indicated above each graph. Post-hoc analyses were performed for one- and two-way ANOVAs where significance between groups is indicated as **p<0.01, ***p<0.001, ****p<0.0001, ns, not significant.

    Article Snippet: Where indicated, BMDM monocultures were activated with 10 ng/mL lipopolysaccharide (LPS; tlrl-3pelps, Invivogen) and 10 ng/mL murine interferon gamma (IFNγ; Abcam), or 10 ng/mL murine interleukin (IL-4; PeproTech) and 10 ng/mL IL-13 (PeproTech), as indicated.

    Techniques: Co-culture Assay, Expressing, Cell Culture, Flow Cytometry, Analysis, Fluorescence