blunt sharp forceps (Fisher Scientific)
90
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Fisher Scientific
blunt sharp forceps
Blunt Sharp Forceps, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blunt+sharp+forceps/stainless+steel+forceps/pmc11717720-57-2-4
Average 90 stars, based on 1 article reviews
Blunt Sharp Forceps, supplied by Fisher Scientific, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blunt+sharp+forceps/stainless+steel+forceps/pmc11717720-57-2-4
Average 90 stars, based on 1 article reviews
blunt sharp forceps - by Bioz Stars,
2026-11
90/100 stars
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Recombinant:Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials 70% (v/v) ethanol (in water) for decontaminating materials and equipment Viable M. oryzae filter frozen stocks (e.g., Guy11; see Basic Protocol 1) Complete medium (CM) sterile plates (see recipe), with or without antibiotics (see step 1) Circulating Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: Clean several sets of forceps (stainless steel blunt-end forceps, Fisherbrand, Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials Viable M. oryzae filter frozen stocks (see Basic Protocol 1) 70% (v/v) ethanol (in water) for decontaminating materials and equipment Complete medium (CM) sterile plates (see recipe) Liquid CM (see recipe) Circulating Class II flow hood (BSC Class II or equivalent) Stainless-steel surgical scalpel handle and scalpel (e.g., Fisher Scientific cat. no. 11890862 and 12961645) Article Title: Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber Article Snippet: Article Title: Cryopreservation Method for Preventing Freeze-Fracture of Small Muscle Samples Article Snippet: Blunt and Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins (DPBS, powder, no calcium, no magnesium) Gibco, Thermo Fisher Scientific #21600010 Benzonase nuclease (HC purity >99%) (if extravesicular DNA digestion is performed) Novagen, Merck #71206 Pierce BCA protein assay Thermo Scientific #23225 Critical commercial assays qEVoriginal/70 nm Gen 2 Column IZON #ICO-70, 1004105 Amicon Ultra-2 Centrifugal Filter Units (Centrifugal concentrator, 3 kDa MWCO, 2 mL sample volume) Merck, Millipore #UFL200324 AMPure XP beads for MV DNA isolation and purification (if DNA isolation for rDNA-seq is performed) Beckman Coulter #A63881 Experimental models: Organisms/strains C57BL/6J Maintained under specific pathogen-free conditions; age- and sex-matched (8–24 weeks), co-housed The Jackson Laboratory #:000664 RRID: IMSR_JAX:000664 Other Nalgene vacuum filtration system with a filter pore size of 0.2 mm Merck #Z358207 Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon Article Snippet: Forceps (stainless steel blunt-end forceps) , Staining:Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials 70% (v/v) ethanol (in water) for decontaminating materials and equipment Viable M. oryzae filter frozen stocks (e.g., Guy11; see Basic Protocol 1) Complete medium (CM) sterile plates (see recipe), with or without antibiotics (see step 1) Circulating Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: Clean several sets of forceps (stainless steel blunt-end forceps, Fisherbrand, Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials Viable M. oryzae filter frozen stocks (see Basic Protocol 1) 70% (v/v) ethanol (in water) for decontaminating materials and equipment Complete medium (CM) sterile plates (see recipe) Liquid CM (see recipe) Circulating Class II flow hood (BSC Class II or equivalent) Stainless-steel surgical scalpel handle and scalpel (e.g., Fisher Scientific cat. no. 11890862 and 12961645) Article Title: Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber Article Snippet: Article Title: Cryopreservation Method for Preventing Freeze-Fracture of Small Muscle Samples Article Snippet: Blunt and Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins (DPBS, powder, no calcium, no magnesium) Gibco, Thermo Fisher Scientific #21600010 Benzonase nuclease (HC purity >99%) (if extravesicular DNA digestion is performed) Novagen, Merck #71206 Pierce BCA protein assay Thermo Scientific #23225 Critical commercial assays qEVoriginal/70 nm Gen 2 Column IZON #ICO-70, 1004105 Amicon Ultra-2 Centrifugal Filter Units (Centrifugal concentrator, 3 kDa MWCO, 2 mL sample volume) Merck, Millipore #UFL200324 AMPure XP beads for MV DNA isolation and purification (if DNA isolation for rDNA-seq is performed) Beckman Coulter #A63881 Experimental models: Organisms/strains C57BL/6J Maintained under specific pathogen-free conditions; age- and sex-matched (8–24 weeks), co-housed The Jackson Laboratory #:000664 RRID: IMSR_JAX:000664 Other Nalgene vacuum filtration system with a filter pore size of 0.2 mm Merck #Z358207 Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon Article Snippet: Forceps (stainless steel blunt-end forceps) , Saline:Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials 70% (v/v) ethanol (in water) for decontaminating materials and equipment Viable M. oryzae filter frozen stocks (e.g., Guy11; see Basic Protocol 1) Complete medium (CM) sterile plates (see recipe), with or without antibiotics (see step 1) Circulating Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: Clean several sets of forceps (stainless steel blunt-end forceps, Fisherbrand, Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials Viable M. oryzae filter frozen stocks (see Basic Protocol 1) 70% (v/v) ethanol (in water) for decontaminating materials and equipment Complete medium (CM) sterile plates (see recipe) Liquid CM (see recipe) Circulating Class II flow hood (BSC Class II or equivalent) Stainless-steel surgical scalpel handle and scalpel (e.g., Fisher Scientific cat. no. 11890862 and 12961645) Article Title: Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber Article Snippet: Article Title: Cryopreservation Method for Preventing Freeze-Fracture of Small Muscle Samples Article Snippet: Blunt and Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins (DPBS, powder, no calcium, no magnesium) Gibco, Thermo Fisher Scientific #21600010 Benzonase nuclease (HC purity >99%) (if extravesicular DNA digestion is performed) Novagen, Merck #71206 Pierce BCA protein assay Thermo Scientific #23225 Critical commercial assays qEVoriginal/70 nm Gen 2 Column IZON #ICO-70, 1004105 Amicon Ultra-2 Centrifugal Filter Units (Centrifugal concentrator, 3 kDa MWCO, 2 mL sample volume) Merck, Millipore #UFL200324 AMPure XP beads for MV DNA isolation and purification (if DNA isolation for rDNA-seq is performed) Beckman Coulter #A63881 Experimental models: Organisms/strains C57BL/6J Maintained under specific pathogen-free conditions; age- and sex-matched (8–24 weeks), co-housed The Jackson Laboratory #:000664 RRID: IMSR_JAX:000664 Other Nalgene vacuum filtration system with a filter pore size of 0.2 mm Merck #Z358207 Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon Article Snippet: Forceps (stainless steel blunt-end forceps) , Protease Inhibitor:Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials 70% (v/v) ethanol (in water) for decontaminating materials and equipment Viable M. oryzae filter frozen stocks (e.g., Guy11; see Basic Protocol 1) Complete medium (CM) sterile plates (see recipe), with or without antibiotics (see step 1) Circulating Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: Clean several sets of forceps (stainless steel blunt-end forceps, Fisherbrand, Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials Viable M. oryzae filter frozen stocks (see Basic Protocol 1) 70% (v/v) ethanol (in water) for decontaminating materials and equipment Complete medium (CM) sterile plates (see recipe) Liquid CM (see recipe) Circulating Class II flow hood (BSC Class II or equivalent) Stainless-steel surgical scalpel handle and scalpel (e.g., Fisher Scientific cat. no. 11890862 and 12961645) Article Title: Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber Article Snippet: Article Title: Cryopreservation Method for Preventing Freeze-Fracture of Small Muscle Samples Article Snippet: Blunt and Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins (DPBS, powder, no calcium, no magnesium) Gibco, Thermo Fisher Scientific #21600010 Benzonase nuclease (HC purity >99%) (if extravesicular DNA digestion is performed) Novagen, Merck #71206 Pierce BCA protein assay Thermo Scientific #23225 Critical commercial assays qEVoriginal/70 nm Gen 2 Column IZON #ICO-70, 1004105 Amicon Ultra-2 Centrifugal Filter Units (Centrifugal concentrator, 3 kDa MWCO, 2 mL sample volume) Merck, Millipore #UFL200324 AMPure XP beads for MV DNA isolation and purification (if DNA isolation for rDNA-seq is performed) Beckman Coulter #A63881 Experimental models: Organisms/strains C57BL/6J Maintained under specific pathogen-free conditions; age- and sex-matched (8–24 weeks), co-housed The Jackson Laboratory #:000664 RRID: IMSR_JAX:000664 Other Nalgene vacuum filtration system with a filter pore size of 0.2 mm Merck #Z358207 Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon Article Snippet: Forceps (stainless steel blunt-end forceps) , Picogreen Assay:Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials 70% (v/v) ethanol (in water) for decontaminating materials and equipment Viable M. oryzae filter frozen stocks (e.g., Guy11; see Basic Protocol 1) Complete medium (CM) sterile plates (see recipe), with or without antibiotics (see step 1) Circulating Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: Clean several sets of forceps (stainless steel blunt-end forceps, Fisherbrand, Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials Viable M. oryzae filter frozen stocks (see Basic Protocol 1) 70% (v/v) ethanol (in water) for decontaminating materials and equipment Complete medium (CM) sterile plates (see recipe) Liquid CM (see recipe) Circulating Class II flow hood (BSC Class II or equivalent) Stainless-steel surgical scalpel handle and scalpel (e.g., Fisher Scientific cat. no. 11890862 and 12961645) Article Title: Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber Article Snippet: Article Title: Cryopreservation Method for Preventing Freeze-Fracture of Small Muscle Samples Article Snippet: Blunt and Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins (DPBS, powder, no calcium, no magnesium) Gibco, Thermo Fisher Scientific #21600010 Benzonase nuclease (HC purity >99%) (if extravesicular DNA digestion is performed) Novagen, Merck #71206 Pierce BCA protein assay Thermo Scientific #23225 Critical commercial assays qEVoriginal/70 nm Gen 2 Column IZON #ICO-70, 1004105 Amicon Ultra-2 Centrifugal Filter Units (Centrifugal concentrator, 3 kDa MWCO, 2 mL sample volume) Merck, Millipore #UFL200324 AMPure XP beads for MV DNA isolation and purification (if DNA isolation for rDNA-seq is performed) Beckman Coulter #A63881 Experimental models: Organisms/strains C57BL/6J Maintained under specific pathogen-free conditions; age- and sex-matched (8–24 weeks), co-housed The Jackson Laboratory #:000664 RRID: IMSR_JAX:000664 Other Nalgene vacuum filtration system with a filter pore size of 0.2 mm Merck #Z358207 Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon Article Snippet: Forceps (stainless steel blunt-end forceps) , Software:Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials 70% (v/v) ethanol (in water) for decontaminating materials and equipment Viable M. oryzae filter frozen stocks (e.g., Guy11; see Basic Protocol 1) Complete medium (CM) sterile plates (see recipe), with or without antibiotics (see step 1) Circulating Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: Clean several sets of forceps (stainless steel blunt-end forceps, Fisherbrand, Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials Viable M. oryzae filter frozen stocks (see Basic Protocol 1) 70% (v/v) ethanol (in water) for decontaminating materials and equipment Complete medium (CM) sterile plates (see recipe) Liquid CM (see recipe) Circulating Class II flow hood (BSC Class II or equivalent) Stainless-steel surgical scalpel handle and scalpel (e.g., Fisher Scientific cat. no. 11890862 and 12961645) Article Title: Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber Article Snippet: Article Title: Cryopreservation Method for Preventing Freeze-Fracture of Small Muscle Samples Article Snippet: Blunt and Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins (DPBS, powder, no calcium, no magnesium) Gibco, Thermo Fisher Scientific #21600010 Benzonase nuclease (HC purity >99%) (if extravesicular DNA digestion is performed) Novagen, Merck #71206 Pierce BCA protein assay Thermo Scientific #23225 Critical commercial assays qEVoriginal/70 nm Gen 2 Column IZON #ICO-70, 1004105 Amicon Ultra-2 Centrifugal Filter Units (Centrifugal concentrator, 3 kDa MWCO, 2 mL sample volume) Merck, Millipore #UFL200324 AMPure XP beads for MV DNA isolation and purification (if DNA isolation for rDNA-seq is performed) Beckman Coulter #A63881 Experimental models: Organisms/strains C57BL/6J Maintained under specific pathogen-free conditions; age- and sex-matched (8–24 weeks), co-housed The Jackson Laboratory #:000664 RRID: IMSR_JAX:000664 Other Nalgene vacuum filtration system with a filter pore size of 0.2 mm Merck #Z358207 Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon Article Snippet: Forceps (stainless steel blunt-end forceps) , Membrane:Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials 70% (v/v) ethanol (in water) for decontaminating materials and equipment Viable M. oryzae filter frozen stocks (e.g., Guy11; see Basic Protocol 1) Complete medium (CM) sterile plates (see recipe), with or without antibiotics (see step 1) Circulating Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: Clean several sets of forceps (stainless steel blunt-end forceps, Fisherbrand, Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials Viable M. oryzae filter frozen stocks (see Basic Protocol 1) 70% (v/v) ethanol (in water) for decontaminating materials and equipment Complete medium (CM) sterile plates (see recipe) Liquid CM (see recipe) Circulating Class II flow hood (BSC Class II or equivalent) Stainless-steel surgical scalpel handle and scalpel (e.g., Fisher Scientific cat. no. 11890862 and 12961645) Article Title: Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber Article Snippet: Article Title: Cryopreservation Method for Preventing Freeze-Fracture of Small Muscle Samples Article Snippet: Blunt and Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins (DPBS, powder, no calcium, no magnesium) Gibco, Thermo Fisher Scientific #21600010 Benzonase nuclease (HC purity >99%) (if extravesicular DNA digestion is performed) Novagen, Merck #71206 Pierce BCA protein assay Thermo Scientific #23225 Critical commercial assays qEVoriginal/70 nm Gen 2 Column IZON #ICO-70, 1004105 Amicon Ultra-2 Centrifugal Filter Units (Centrifugal concentrator, 3 kDa MWCO, 2 mL sample volume) Merck, Millipore #UFL200324 AMPure XP beads for MV DNA isolation and purification (if DNA isolation for rDNA-seq is performed) Beckman Coulter #A63881 Experimental models: Organisms/strains C57BL/6J Maintained under specific pathogen-free conditions; age- and sex-matched (8–24 weeks), co-housed The Jackson Laboratory #:000664 RRID: IMSR_JAX:000664 Other Nalgene vacuum filtration system with a filter pore size of 0.2 mm Merck #Z358207 Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon Article Snippet: Forceps (stainless steel blunt-end forceps) , Adhesive:Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials 70% (v/v) ethanol (in water) for decontaminating materials and equipment Viable M. oryzae filter frozen stocks (e.g., Guy11; see Basic Protocol 1) Complete medium (CM) sterile plates (see recipe), with or without antibiotics (see step 1) Circulating Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: Clean several sets of forceps (stainless steel blunt-end forceps, Fisherbrand, Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials Viable M. oryzae filter frozen stocks (see Basic Protocol 1) 70% (v/v) ethanol (in water) for decontaminating materials and equipment Complete medium (CM) sterile plates (see recipe) Liquid CM (see recipe) Circulating Class II flow hood (BSC Class II or equivalent) Stainless-steel surgical scalpel handle and scalpel (e.g., Fisher Scientific cat. no. 11890862 and 12961645) Article Title: Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber Article Snippet: Article Title: Cryopreservation Method for Preventing Freeze-Fracture of Small Muscle Samples Article Snippet: Blunt and Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins (DPBS, powder, no calcium, no magnesium) Gibco, Thermo Fisher Scientific #21600010 Benzonase nuclease (HC purity >99%) (if extravesicular DNA digestion is performed) Novagen, Merck #71206 Pierce BCA protein assay Thermo Scientific #23225 Critical commercial assays qEVoriginal/70 nm Gen 2 Column IZON #ICO-70, 1004105 Amicon Ultra-2 Centrifugal Filter Units (Centrifugal concentrator, 3 kDa MWCO, 2 mL sample volume) Merck, Millipore #UFL200324 AMPure XP beads for MV DNA isolation and purification (if DNA isolation for rDNA-seq is performed) Beckman Coulter #A63881 Experimental models: Organisms/strains C57BL/6J Maintained under specific pathogen-free conditions; age- and sex-matched (8–24 weeks), co-housed The Jackson Laboratory #:000664 RRID: IMSR_JAX:000664 Other Nalgene vacuum filtration system with a filter pore size of 0.2 mm Merck #Z358207 Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon Article Snippet: Forceps (stainless steel blunt-end forceps) , Blocking Assay:Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials 70% (v/v) ethanol (in water) for decontaminating materials and equipment Viable M. oryzae filter frozen stocks (e.g., Guy11; see Basic Protocol 1) Complete medium (CM) sterile plates (see recipe), with or without antibiotics (see step 1) Circulating Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: Clean several sets of forceps (stainless steel blunt-end forceps, Fisherbrand, Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials Viable M. oryzae filter frozen stocks (see Basic Protocol 1) 70% (v/v) ethanol (in water) for decontaminating materials and equipment Complete medium (CM) sterile plates (see recipe) Liquid CM (see recipe) Circulating Class II flow hood (BSC Class II or equivalent) Stainless-steel surgical scalpel handle and scalpel (e.g., Fisher Scientific cat. no. 11890862 and 12961645) Article Title: Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber Article Snippet: Article Title: Cryopreservation Method for Preventing Freeze-Fracture of Small Muscle Samples Article Snippet: Blunt and Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins (DPBS, powder, no calcium, no magnesium) Gibco, Thermo Fisher Scientific #21600010 Benzonase nuclease (HC purity >99%) (if extravesicular DNA digestion is performed) Novagen, Merck #71206 Pierce BCA protein assay Thermo Scientific #23225 Critical commercial assays qEVoriginal/70 nm Gen 2 Column IZON #ICO-70, 1004105 Amicon Ultra-2 Centrifugal Filter Units (Centrifugal concentrator, 3 kDa MWCO, 2 mL sample volume) Merck, Millipore #UFL200324 AMPure XP beads for MV DNA isolation and purification (if DNA isolation for rDNA-seq is performed) Beckman Coulter #A63881 Experimental models: Organisms/strains C57BL/6J Maintained under specific pathogen-free conditions; age- and sex-matched (8–24 weeks), co-housed The Jackson Laboratory #:000664 RRID: IMSR_JAX:000664 Other Nalgene vacuum filtration system with a filter pore size of 0.2 mm Merck #Z358207 Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon Article Snippet: Forceps (stainless steel blunt-end forceps) , Microscopy:Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials 70% (v/v) ethanol (in water) for decontaminating materials and equipment Viable M. oryzae filter frozen stocks (e.g., Guy11; see Basic Protocol 1) Complete medium (CM) sterile plates (see recipe), with or without antibiotics (see step 1) Circulating Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: Clean several sets of forceps (stainless steel blunt-end forceps, Fisherbrand, Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials Viable M. oryzae filter frozen stocks (see Basic Protocol 1) 70% (v/v) ethanol (in water) for decontaminating materials and equipment Complete medium (CM) sterile plates (see recipe) Liquid CM (see recipe) Circulating Class II flow hood (BSC Class II or equivalent) Stainless-steel surgical scalpel handle and scalpel (e.g., Fisher Scientific cat. no. 11890862 and 12961645) Article Title: Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber Article Snippet: Article Title: Cryopreservation Method for Preventing Freeze-Fracture of Small Muscle Samples Article Snippet: Blunt and Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins (DPBS, powder, no calcium, no magnesium) Gibco, Thermo Fisher Scientific #21600010 Benzonase nuclease (HC purity >99%) (if extravesicular DNA digestion is performed) Novagen, Merck #71206 Pierce BCA protein assay Thermo Scientific #23225 Critical commercial assays qEVoriginal/70 nm Gen 2 Column IZON #ICO-70, 1004105 Amicon Ultra-2 Centrifugal Filter Units (Centrifugal concentrator, 3 kDa MWCO, 2 mL sample volume) Merck, Millipore #UFL200324 AMPure XP beads for MV DNA isolation and purification (if DNA isolation for rDNA-seq is performed) Beckman Coulter #A63881 Experimental models: Organisms/strains C57BL/6J Maintained under specific pathogen-free conditions; age- and sex-matched (8–24 weeks), co-housed The Jackson Laboratory #:000664 RRID: IMSR_JAX:000664 Other Nalgene vacuum filtration system with a filter pore size of 0.2 mm Merck #Z358207 Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon Article Snippet: Forceps (stainless steel blunt-end forceps) , Electron Microscopy:Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials 70% (v/v) ethanol (in water) for decontaminating materials and equipment Viable M. oryzae filter frozen stocks (e.g., Guy11; see Basic Protocol 1) Complete medium (CM) sterile plates (see recipe), with or without antibiotics (see step 1) Circulating Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: Clean several sets of forceps (stainless steel blunt-end forceps, Fisherbrand, Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials Viable M. oryzae filter frozen stocks (see Basic Protocol 1) 70% (v/v) ethanol (in water) for decontaminating materials and equipment Complete medium (CM) sterile plates (see recipe) Liquid CM (see recipe) Circulating Class II flow hood (BSC Class II or equivalent) Stainless-steel surgical scalpel handle and scalpel (e.g., Fisher Scientific cat. no. 11890862 and 12961645) Article Title: Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber Article Snippet: Article Title: Cryopreservation Method for Preventing Freeze-Fracture of Small Muscle Samples Article Snippet: Blunt and Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins (DPBS, powder, no calcium, no magnesium) Gibco, Thermo Fisher Scientific #21600010 Benzonase nuclease (HC purity >99%) (if extravesicular DNA digestion is performed) Novagen, Merck #71206 Pierce BCA protein assay Thermo Scientific #23225 Critical commercial assays qEVoriginal/70 nm Gen 2 Column IZON #ICO-70, 1004105 Amicon Ultra-2 Centrifugal Filter Units (Centrifugal concentrator, 3 kDa MWCO, 2 mL sample volume) Merck, Millipore #UFL200324 AMPure XP beads for MV DNA isolation and purification (if DNA isolation for rDNA-seq is performed) Beckman Coulter #A63881 Experimental models: Organisms/strains C57BL/6J Maintained under specific pathogen-free conditions; age- and sex-matched (8–24 weeks), co-housed The Jackson Laboratory #:000664 RRID: IMSR_JAX:000664 Other Nalgene vacuum filtration system with a filter pore size of 0.2 mm Merck #Z358207 Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon Article Snippet: Forceps (stainless steel blunt-end forceps) , Bicinchoninic Acid Protein Assay:Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials 70% (v/v) ethanol (in water) for decontaminating materials and equipment Viable M. oryzae filter frozen stocks (e.g., Guy11; see Basic Protocol 1) Complete medium (CM) sterile plates (see recipe), with or without antibiotics (see step 1) Circulating Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: Clean several sets of forceps (stainless steel blunt-end forceps, Fisherbrand, Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials Viable M. oryzae filter frozen stocks (see Basic Protocol 1) 70% (v/v) ethanol (in water) for decontaminating materials and equipment Complete medium (CM) sterile plates (see recipe) Liquid CM (see recipe) Circulating Class II flow hood (BSC Class II or equivalent) Stainless-steel surgical scalpel handle and scalpel (e.g., Fisher Scientific cat. no. 11890862 and 12961645) Article Title: Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber Article Snippet: Article Title: Cryopreservation Method for Preventing Freeze-Fracture of Small Muscle Samples Article Snippet: Blunt and Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins (DPBS, powder, no calcium, no magnesium) Gibco, Thermo Fisher Scientific #21600010 Benzonase nuclease (HC purity >99%) (if extravesicular DNA digestion is performed) Novagen, Merck #71206 Pierce BCA protein assay Thermo Scientific #23225 Critical commercial assays qEVoriginal/70 nm Gen 2 Column IZON #ICO-70, 1004105 Amicon Ultra-2 Centrifugal Filter Units (Centrifugal concentrator, 3 kDa MWCO, 2 mL sample volume) Merck, Millipore #UFL200324 AMPure XP beads for MV DNA isolation and purification (if DNA isolation for rDNA-seq is performed) Beckman Coulter #A63881 Experimental models: Organisms/strains C57BL/6J Maintained under specific pathogen-free conditions; age- and sex-matched (8–24 weeks), co-housed The Jackson Laboratory #:000664 RRID: IMSR_JAX:000664 Other Nalgene vacuum filtration system with a filter pore size of 0.2 mm Merck #Z358207 Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon Article Snippet: Forceps (stainless steel blunt-end forceps) , DNA Extraction:Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials 70% (v/v) ethanol (in water) for decontaminating materials and equipment Viable M. oryzae filter frozen stocks (e.g., Guy11; see Basic Protocol 1) Complete medium (CM) sterile plates (see recipe), with or without antibiotics (see step 1) Circulating Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: Clean several sets of forceps (stainless steel blunt-end forceps, Fisherbrand, Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials Viable M. oryzae filter frozen stocks (see Basic Protocol 1) 70% (v/v) ethanol (in water) for decontaminating materials and equipment Complete medium (CM) sterile plates (see recipe) Liquid CM (see recipe) Circulating Class II flow hood (BSC Class II or equivalent) Stainless-steel surgical scalpel handle and scalpel (e.g., Fisher Scientific cat. no. 11890862 and 12961645) Article Title: Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber Article Snippet: Article Title: Cryopreservation Method for Preventing Freeze-Fracture of Small Muscle Samples Article Snippet: Blunt and Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins (DPBS, powder, no calcium, no magnesium) Gibco, Thermo Fisher Scientific #21600010 Benzonase nuclease (HC purity >99%) (if extravesicular DNA digestion is performed) Novagen, Merck #71206 Pierce BCA protein assay Thermo Scientific #23225 Critical commercial assays qEVoriginal/70 nm Gen 2 Column IZON #ICO-70, 1004105 Amicon Ultra-2 Centrifugal Filter Units (Centrifugal concentrator, 3 kDa MWCO, 2 mL sample volume) Merck, Millipore #UFL200324 AMPure XP beads for MV DNA isolation and purification (if DNA isolation for rDNA-seq is performed) Beckman Coulter #A63881 Experimental models: Organisms/strains C57BL/6J Maintained under specific pathogen-free conditions; age- and sex-matched (8–24 weeks), co-housed The Jackson Laboratory #:000664 RRID: IMSR_JAX:000664 Other Nalgene vacuum filtration system with a filter pore size of 0.2 mm Merck #Z358207 Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon Article Snippet: Forceps (stainless steel blunt-end forceps) , Purification:Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials 70% (v/v) ethanol (in water) for decontaminating materials and equipment Viable M. oryzae filter frozen stocks (e.g., Guy11; see Basic Protocol 1) Complete medium (CM) sterile plates (see recipe), with or without antibiotics (see step 1) Circulating Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: Clean several sets of forceps (stainless steel blunt-end forceps, Fisherbrand, Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials Viable M. oryzae filter frozen stocks (see Basic Protocol 1) 70% (v/v) ethanol (in water) for decontaminating materials and equipment Complete medium (CM) sterile plates (see recipe) Liquid CM (see recipe) Circulating Class II flow hood (BSC Class II or equivalent) Stainless-steel surgical scalpel handle and scalpel (e.g., Fisher Scientific cat. no. 11890862 and 12961645) Article Title: Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber Article Snippet: Article Title: Cryopreservation Method for Preventing Freeze-Fracture of Small Muscle Samples Article Snippet: Blunt and Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins (DPBS, powder, no calcium, no magnesium) Gibco, Thermo Fisher Scientific #21600010 Benzonase nuclease (HC purity >99%) (if extravesicular DNA digestion is performed) Novagen, Merck #71206 Pierce BCA protein assay Thermo Scientific #23225 Critical commercial assays qEVoriginal/70 nm Gen 2 Column IZON #ICO-70, 1004105 Amicon Ultra-2 Centrifugal Filter Units (Centrifugal concentrator, 3 kDa MWCO, 2 mL sample volume) Merck, Millipore #UFL200324 AMPure XP beads for MV DNA isolation and purification (if DNA isolation for rDNA-seq is performed) Beckman Coulter #A63881 Experimental models: Organisms/strains C57BL/6J Maintained under specific pathogen-free conditions; age- and sex-matched (8–24 weeks), co-housed The Jackson Laboratory #:000664 RRID: IMSR_JAX:000664 Other Nalgene vacuum filtration system with a filter pore size of 0.2 mm Merck #Z358207 Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon Article Snippet: Forceps (stainless steel blunt-end forceps) , Suction Filtration:Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials 70% (v/v) ethanol (in water) for decontaminating materials and equipment Viable M. oryzae filter frozen stocks (e.g., Guy11; see Basic Protocol 1) Complete medium (CM) sterile plates (see recipe), with or without antibiotics (see step 1) Circulating Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: Clean several sets of forceps (stainless steel blunt-end forceps, Fisherbrand, Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials Viable M. oryzae filter frozen stocks (see Basic Protocol 1) 70% (v/v) ethanol (in water) for decontaminating materials and equipment Complete medium (CM) sterile plates (see recipe) Liquid CM (see recipe) Circulating Class II flow hood (BSC Class II or equivalent) Stainless-steel surgical scalpel handle and scalpel (e.g., Fisher Scientific cat. no. 11890862 and 12961645) Article Title: Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber Article Snippet: Article Title: Cryopreservation Method for Preventing Freeze-Fracture of Small Muscle Samples Article Snippet: Blunt and Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins (DPBS, powder, no calcium, no magnesium) Gibco, Thermo Fisher Scientific #21600010 Benzonase nuclease (HC purity >99%) (if extravesicular DNA digestion is performed) Novagen, Merck #71206 Pierce BCA protein assay Thermo Scientific #23225 Critical commercial assays qEVoriginal/70 nm Gen 2 Column IZON #ICO-70, 1004105 Amicon Ultra-2 Centrifugal Filter Units (Centrifugal concentrator, 3 kDa MWCO, 2 mL sample volume) Merck, Millipore #UFL200324 AMPure XP beads for MV DNA isolation and purification (if DNA isolation for rDNA-seq is performed) Beckman Coulter #A63881 Experimental models: Organisms/strains C57BL/6J Maintained under specific pathogen-free conditions; age- and sex-matched (8–24 weeks), co-housed The Jackson Laboratory #:000664 RRID: IMSR_JAX:000664 Other Nalgene vacuum filtration system with a filter pore size of 0.2 mm Merck #Z358207 Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon Article Snippet: Forceps (stainless steel blunt-end forceps) , Pore Size:Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials 70% (v/v) ethanol (in water) for decontaminating materials and equipment Viable M. oryzae filter frozen stocks (e.g., Guy11; see Basic Protocol 1) Complete medium (CM) sterile plates (see recipe), with or without antibiotics (see step 1) Circulating Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: Clean several sets of forceps (stainless steel blunt-end forceps, Fisherbrand, Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials Viable M. oryzae filter frozen stocks (see Basic Protocol 1) 70% (v/v) ethanol (in water) for decontaminating materials and equipment Complete medium (CM) sterile plates (see recipe) Liquid CM (see recipe) Circulating Class II flow hood (BSC Class II or equivalent) Stainless-steel surgical scalpel handle and scalpel (e.g., Fisher Scientific cat. no. 11890862 and 12961645) Article Title: Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber Article Snippet: Article Title: Cryopreservation Method for Preventing Freeze-Fracture of Small Muscle Samples Article Snippet: Blunt and Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins (DPBS, powder, no calcium, no magnesium) Gibco, Thermo Fisher Scientific #21600010 Benzonase nuclease (HC purity >99%) (if extravesicular DNA digestion is performed) Novagen, Merck #71206 Pierce BCA protein assay Thermo Scientific #23225 Critical commercial assays qEVoriginal/70 nm Gen 2 Column IZON #ICO-70, 1004105 Amicon Ultra-2 Centrifugal Filter Units (Centrifugal concentrator, 3 kDa MWCO, 2 mL sample volume) Merck, Millipore #UFL200324 AMPure XP beads for MV DNA isolation and purification (if DNA isolation for rDNA-seq is performed) Beckman Coulter #A63881 Experimental models: Organisms/strains C57BL/6J Maintained under specific pathogen-free conditions; age- and sex-matched (8–24 weeks), co-housed The Jackson Laboratory #:000664 RRID: IMSR_JAX:000664 Other Nalgene vacuum filtration system with a filter pore size of 0.2 mm Merck #Z358207 Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon Article Snippet: Forceps (stainless steel blunt-end forceps) , Sterility:Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials 70% (v/v) ethanol (in water) for decontaminating materials and equipment Viable M. oryzae filter frozen stocks (e.g., Guy11; see Basic Protocol 1) Complete medium (CM) sterile plates (see recipe), with or without antibiotics (see step 1) Circulating Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: Clean several sets of forceps (stainless steel blunt-end forceps, Fisherbrand, Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials Viable M. oryzae filter frozen stocks (see Basic Protocol 1) 70% (v/v) ethanol (in water) for decontaminating materials and equipment Complete medium (CM) sterile plates (see recipe) Liquid CM (see recipe) Circulating Class II flow hood (BSC Class II or equivalent) Stainless-steel surgical scalpel handle and scalpel (e.g., Fisher Scientific cat. no. 11890862 and 12961645) Article Title: Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber Article Snippet: Article Title: Cryopreservation Method for Preventing Freeze-Fracture of Small Muscle Samples Article Snippet: Blunt and Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins (DPBS, powder, no calcium, no magnesium) Gibco, Thermo Fisher Scientific #21600010 Benzonase nuclease (HC purity >99%) (if extravesicular DNA digestion is performed) Novagen, Merck #71206 Pierce BCA protein assay Thermo Scientific #23225 Critical commercial assays qEVoriginal/70 nm Gen 2 Column IZON #ICO-70, 1004105 Amicon Ultra-2 Centrifugal Filter Units (Centrifugal concentrator, 3 kDa MWCO, 2 mL sample volume) Merck, Millipore #UFL200324 AMPure XP beads for MV DNA isolation and purification (if DNA isolation for rDNA-seq is performed) Beckman Coulter #A63881 Experimental models: Organisms/strains C57BL/6J Maintained under specific pathogen-free conditions; age- and sex-matched (8–24 weeks), co-housed The Jackson Laboratory #:000664 RRID: IMSR_JAX:000664 Other Nalgene vacuum filtration system with a filter pore size of 0.2 mm Merck #Z358207 Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon Article Snippet: Forceps (stainless steel blunt-end forceps) , Low Protein Binding:Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials 70% (v/v) ethanol (in water) for decontaminating materials and equipment Viable M. oryzae filter frozen stocks (e.g., Guy11; see Basic Protocol 1) Complete medium (CM) sterile plates (see recipe), with or without antibiotics (see step 1) Circulating Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: Clean several sets of forceps (stainless steel blunt-end forceps, Fisherbrand, Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials Viable M. oryzae filter frozen stocks (see Basic Protocol 1) 70% (v/v) ethanol (in water) for decontaminating materials and equipment Complete medium (CM) sterile plates (see recipe) Liquid CM (see recipe) Circulating Class II flow hood (BSC Class II or equivalent) Stainless-steel surgical scalpel handle and scalpel (e.g., Fisher Scientific cat. no. 11890862 and 12961645) Article Title: Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber Article Snippet: Article Title: Cryopreservation Method for Preventing Freeze-Fracture of Small Muscle Samples Article Snippet: Blunt and Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins (DPBS, powder, no calcium, no magnesium) Gibco, Thermo Fisher Scientific #21600010 Benzonase nuclease (HC purity >99%) (if extravesicular DNA digestion is performed) Novagen, Merck #71206 Pierce BCA protein assay Thermo Scientific #23225 Critical commercial assays qEVoriginal/70 nm Gen 2 Column IZON #ICO-70, 1004105 Amicon Ultra-2 Centrifugal Filter Units (Centrifugal concentrator, 3 kDa MWCO, 2 mL sample volume) Merck, Millipore #UFL200324 AMPure XP beads for MV DNA isolation and purification (if DNA isolation for rDNA-seq is performed) Beckman Coulter #A63881 Experimental models: Organisms/strains C57BL/6J Maintained under specific pathogen-free conditions; age- and sex-matched (8–24 weeks), co-housed The Jackson Laboratory #:000664 RRID: IMSR_JAX:000664 Other Nalgene vacuum filtration system with a filter pore size of 0.2 mm Merck #Z358207 Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon Article Snippet: Forceps (stainless steel blunt-end forceps) , Transferring:Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials 70% (v/v) ethanol (in water) for decontaminating materials and equipment Viable M. oryzae filter frozen stocks (e.g., Guy11; see Basic Protocol 1) Complete medium (CM) sterile plates (see recipe), with or without antibiotics (see step 1) Circulating Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: Clean several sets of forceps (stainless steel blunt-end forceps, Fisherbrand, Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials Viable M. oryzae filter frozen stocks (see Basic Protocol 1) 70% (v/v) ethanol (in water) for decontaminating materials and equipment Complete medium (CM) sterile plates (see recipe) Liquid CM (see recipe) Circulating Class II flow hood (BSC Class II or equivalent) Stainless-steel surgical scalpel handle and scalpel (e.g., Fisher Scientific cat. no. 11890862 and 12961645) Article Title: Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber Article Snippet: Article Title: Cryopreservation Method for Preventing Freeze-Fracture of Small Muscle Samples Article Snippet: Blunt and Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins (DPBS, powder, no calcium, no magnesium) Gibco, Thermo Fisher Scientific #21600010 Benzonase nuclease (HC purity >99%) (if extravesicular DNA digestion is performed) Novagen, Merck #71206 Pierce BCA protein assay Thermo Scientific #23225 Critical commercial assays qEVoriginal/70 nm Gen 2 Column IZON #ICO-70, 1004105 Amicon Ultra-2 Centrifugal Filter Units (Centrifugal concentrator, 3 kDa MWCO, 2 mL sample volume) Merck, Millipore #UFL200324 AMPure XP beads for MV DNA isolation and purification (if DNA isolation for rDNA-seq is performed) Beckman Coulter #A63881 Experimental models: Organisms/strains C57BL/6J Maintained under specific pathogen-free conditions; age- and sex-matched (8–24 weeks), co-housed The Jackson Laboratory #:000664 RRID: IMSR_JAX:000664 Other Nalgene vacuum filtration system with a filter pore size of 0.2 mm Merck #Z358207 Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon Article Snippet: Forceps (stainless steel blunt-end forceps) , Convection:Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials 70% (v/v) ethanol (in water) for decontaminating materials and equipment Viable M. oryzae filter frozen stocks (e.g., Guy11; see Basic Protocol 1) Complete medium (CM) sterile plates (see recipe), with or without antibiotics (see step 1) Circulating Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: Clean several sets of forceps (stainless steel blunt-end forceps, Fisherbrand, Article Title: A Basic Guide to the Growth and Manipulation of the Blast Fungus, Magnaporthe oryzae. Article Snippet: Materials Viable M. oryzae filter frozen stocks (see Basic Protocol 1) 70% (v/v) ethanol (in water) for decontaminating materials and equipment Complete medium (CM) sterile plates (see recipe) Liquid CM (see recipe) Circulating Class II flow hood (BSC Class II or equivalent) Stainless-steel surgical scalpel handle and scalpel (e.g., Fisher Scientific cat. no. 11890862 and 12961645) Article Title: Protocol to decellularize porcine right ventricular outflow tracts using a 3D printed flow chamber Article Snippet: Article Title: Cryopreservation Method for Preventing Freeze-Fracture of Small Muscle Samples Article Snippet: Blunt and Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon. Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals, peptides, and recombinant proteins (DPBS, powder, no calcium, no magnesium) Gibco, Thermo Fisher Scientific #21600010 Benzonase nuclease (HC purity >99%) (if extravesicular DNA digestion is performed) Novagen, Merck #71206 Pierce BCA protein assay Thermo Scientific #23225 Critical commercial assays qEVoriginal/70 nm Gen 2 Column IZON #ICO-70, 1004105 Amicon Ultra-2 Centrifugal Filter Units (Centrifugal concentrator, 3 kDa MWCO, 2 mL sample volume) Merck, Millipore #UFL200324 AMPure XP beads for MV DNA isolation and purification (if DNA isolation for rDNA-seq is performed) Beckman Coulter #A63881 Experimental models: Organisms/strains C57BL/6J Maintained under specific pathogen-free conditions; age- and sex-matched (8–24 weeks), co-housed The Jackson Laboratory #:000664 RRID: IMSR_JAX:000664 Other Nalgene vacuum filtration system with a filter pore size of 0.2 mm Merck #Z358207 Article Title: Protocol for isolation of microbiota-derived membrane vesicles from mouse blood and colon Article Snippet: Forceps (stainless steel blunt-end forceps) , |