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bluescript  (Agilent technologies)


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    Structured Review

    Agilent technologies bluescript
    Bluescript, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bluescript/us12065495-1418-18-19
    Average 90 stars, based on 1 article reviews
    bluescript - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Cloning:

    Article Title: Isolation and molecular analysis of Umhda2 a gene encoding a histone deacetylase from Ustilago maydis.
    Article Snippet: By use of the polymerase chain reaction and synthetic oligonucleotides designed from conserved regions, we amplified a fragment of a gene from Ustilago maydis encoding a putative histone deacetylase.. With this probe we isolated the full gene from a minigenomic library.. The gene (designated as Umhda2) contains an open reading frame (ORF) of 1701 bp encoding a protein of 566 amino acids.

    Article Title: Compositions and methods comprising anti-NRP2 antibodies
    Article Snippet: Such vectors include, but are not limited to, the multifunctional E. coli cloning and expression vectors such as BLUESCRIPT (Stratagene), in which the sequence encoding the polypeptide of interest may be ligated into the vector in frame with sequences for the amino-terminal Met and the subsequent 7 residues of β-galactosidase so that a hybrid protein is produced; pIN vectors (Van Heeke & Schuster, J. Biol. .. Such vectors include, but are not limited to, the multifunctional E. coli cloning and expression vectors such as BLUESCRIPT (Stratagene), in which the sequence encoding the polypeptide of interest may be ligated into the vector in frame with sequences for the amino-terminal Met and the subsequent 7 residues of β-galactosidase so that a hybrid protein is produced; pIN vectors (Van Heeke & Schuster, J. Biol. .. 264:5503 5509 (1989)); and the like. pGEX Vectors (Promega, Madison, Wis.) may also be used to express foreign polypeptides as fusion proteins with glutathione S-transferase (GST).

    Plasmid Preparation:

    Article Title: Isolation and molecular analysis of Umhda2 a gene encoding a histone deacetylase from Ustilago maydis.
    Article Snippet: By use of the polymerase chain reaction and synthetic oligonucleotides designed from conserved regions, we amplified a fragment of a gene from Ustilago maydis encoding a putative histone deacetylase.. With this probe we isolated the full gene from a minigenomic library.. The gene (designated as Umhda2) contains an open reading frame (ORF) of 1701 bp encoding a protein of 566 amino acids.

    Article Title: Compositions and methods comprising anti-NRP2 antibodies
    Article Snippet: Such vectors include, but are not limited to, the multifunctional E. coli cloning and expression vectors such as BLUESCRIPT (Stratagene), in which the sequence encoding the polypeptide of interest may be ligated into the vector in frame with sequences for the amino-terminal Met and the subsequent 7 residues of β-galactosidase so that a hybrid protein is produced; pIN vectors (Van Heeke & Schuster, J. Biol. .. Such vectors include, but are not limited to, the multifunctional E. coli cloning and expression vectors such as BLUESCRIPT (Stratagene), in which the sequence encoding the polypeptide of interest may be ligated into the vector in frame with sequences for the amino-terminal Met and the subsequent 7 residues of β-galactosidase so that a hybrid protein is produced; pIN vectors (Van Heeke & Schuster, J. Biol. .. 264:5503 5509 (1989)); and the like. pGEX Vectors (Promega, Madison, Wis.) may also be used to express foreign polypeptides as fusion proteins with glutathione S-transferase (GST).

    Article Title: Species-Specific Elements in the Large T-Antigen J Domain Are Required for Cellular Transformation and DNA Replication by Simian Virus 40
    Article Snippet: .. The J domain for each chimera was PCR amplified and cloned into the Bgl II and Apa I sites in the polylinker of pSKB, a Bluescript (Stratagene)-derived vector in which the Pst I site was replaced with a Bgl II site. ..

    other:

    Article Title: mRNA Molecules Containing Murine Leukemia Virus Packaging Signals Are Encapsidated as Dimers
    Article Snippet: An HaSV-specific riboprobe template, complementary to the v- ras H gene, was made by XbaI digestion of a Bluescript (Stratagene)-based plasmid containing the insert of pBS9 ( 19 ).

    Article Title: An Efficient Method to Generate Chromosomal Rearrangements by Targeted DNA Double-Strand Breaks in Drosophila melanogaster
    Article Snippet: DNA Constructs DE16 was constructed by using a Bluescript (Stratagene)-based vector containing two loxP sites (a gift from K. Basler, University of Zurich, Zurich, Switzerland).

    Article Title: An Efficient Method to Generate Chromosomal Rearrangements by Targeted DNA Double-Strand Breaks in Drosophila melanogaster
    Article Snippet: DE16 was constructed by using a Bluescript (Stratagene)-based vector containing two loxP sites (a gift from K. Basler, University of Zurich, Zurich, Switzerland).

    Expressing:

    Article Title: Compositions and methods comprising anti-NRP2 antibodies
    Article Snippet: Such vectors include, but are not limited to, the multifunctional E. coli cloning and expression vectors such as BLUESCRIPT (Stratagene), in which the sequence encoding the polypeptide of interest may be ligated into the vector in frame with sequences for the amino-terminal Met and the subsequent 7 residues of β-galactosidase so that a hybrid protein is produced; pIN vectors (Van Heeke & Schuster, J. Biol. .. Such vectors include, but are not limited to, the multifunctional E. coli cloning and expression vectors such as BLUESCRIPT (Stratagene), in which the sequence encoding the polypeptide of interest may be ligated into the vector in frame with sequences for the amino-terminal Met and the subsequent 7 residues of β-galactosidase so that a hybrid protein is produced; pIN vectors (Van Heeke & Schuster, J. Biol. .. 264:5503 5509 (1989)); and the like. pGEX Vectors (Promega, Madison, Wis.) may also be used to express foreign polypeptides as fusion proteins with glutathione S-transferase (GST).

    Sequencing:

    Article Title: Compositions and methods comprising anti-NRP2 antibodies
    Article Snippet: Such vectors include, but are not limited to, the multifunctional E. coli cloning and expression vectors such as BLUESCRIPT (Stratagene), in which the sequence encoding the polypeptide of interest may be ligated into the vector in frame with sequences for the amino-terminal Met and the subsequent 7 residues of β-galactosidase so that a hybrid protein is produced; pIN vectors (Van Heeke & Schuster, J. Biol. .. Such vectors include, but are not limited to, the multifunctional E. coli cloning and expression vectors such as BLUESCRIPT (Stratagene), in which the sequence encoding the polypeptide of interest may be ligated into the vector in frame with sequences for the amino-terminal Met and the subsequent 7 residues of β-galactosidase so that a hybrid protein is produced; pIN vectors (Van Heeke & Schuster, J. Biol. .. 264:5503 5509 (1989)); and the like. pGEX Vectors (Promega, Madison, Wis.) may also be used to express foreign polypeptides as fusion proteins with glutathione S-transferase (GST).

    Produced:

    Article Title: Compositions and methods comprising anti-NRP2 antibodies
    Article Snippet: Such vectors include, but are not limited to, the multifunctional E. coli cloning and expression vectors such as BLUESCRIPT (Stratagene), in which the sequence encoding the polypeptide of interest may be ligated into the vector in frame with sequences for the amino-terminal Met and the subsequent 7 residues of β-galactosidase so that a hybrid protein is produced; pIN vectors (Van Heeke & Schuster, J. Biol. .. Such vectors include, but are not limited to, the multifunctional E. coli cloning and expression vectors such as BLUESCRIPT (Stratagene), in which the sequence encoding the polypeptide of interest may be ligated into the vector in frame with sequences for the amino-terminal Met and the subsequent 7 residues of β-galactosidase so that a hybrid protein is produced; pIN vectors (Van Heeke & Schuster, J. Biol. .. 264:5503 5509 (1989)); and the like. pGEX Vectors (Promega, Madison, Wis.) may also be used to express foreign polypeptides as fusion proteins with glutathione S-transferase (GST).

    Polymerase Chain Reaction:

    Article Title: Species-Specific Elements in the Large T-Antigen J Domain Are Required for Cellular Transformation and DNA Replication by Simian Virus 40
    Article Snippet: .. The J domain for each chimera was PCR amplified and cloned into the Bgl II and Apa I sites in the polylinker of pSKB, a Bluescript (Stratagene)-derived vector in which the Pst I site was replaced with a Bgl II site. ..

    Amplification:

    Article Title: Species-Specific Elements in the Large T-Antigen J Domain Are Required for Cellular Transformation and DNA Replication by Simian Virus 40
    Article Snippet: .. The J domain for each chimera was PCR amplified and cloned into the Bgl II and Apa I sites in the polylinker of pSKB, a Bluescript (Stratagene)-derived vector in which the Pst I site was replaced with a Bgl II site. ..

    Clone Assay:

    Article Title: Species-Specific Elements in the Large T-Antigen J Domain Are Required for Cellular Transformation and DNA Replication by Simian Virus 40
    Article Snippet: .. The J domain for each chimera was PCR amplified and cloned into the Bgl II and Apa I sites in the polylinker of pSKB, a Bluescript (Stratagene)-derived vector in which the Pst I site was replaced with a Bgl II site. ..



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