upar probe (ATCC)
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90
Structured Review
ATCC
upar probe
Upar Probe, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bluescript/uPAR%2FBluescript/pmc00084441-196-14-21
Average 90 stars, based on 4 article reviews
Upar Probe, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/bluescript/uPAR%2FBluescript/pmc00084441-196-14-21
Average 90 stars, based on 4 article reviews
upar probe - by Bioz Stars,
2026-11
90/100 stars
Images
1) Product Images from "The von Hippel-Lindau Tumor Suppressor Gene Inhibits Hepatocyte Growth Factor/Scatter Factor-Induced Invasion and Branching Morphogenesis in Renal Carcinoma Cells"
Article Title: The von Hippel-Lindau Tumor Suppressor Gene Inhibits Hepatocyte Growth Factor/Scatter Factor-Induced Invasion and Branching Morphogenesis in Renal Carcinoma Cells
Journal:
doi:
Figure Legend Snippet: uPA and uPAR induction in 786-0 RCC cells. Subconfluent cultures of cells were incubated for 7 h at 37°C in DMEM–10% FBS supplemented with 200 ng of HGF/SF per ml or unsupplemented. Total RNA extraction and Northern hybridization were performed as described in Materials and Methods. The blots were probed or reprobed with a 32P-labeled human uPA probe, a human uPAR probe, and a human glyceraldehyde 3-phosphate dehydrogenase (GAPDH) probe (52). Mut, VHL-negative 786-0 RCC cells; WT, VHL-positive 786-0 RCC cells.
Techniques Used: Incubation, RNA Extraction, Northern Blot, Hybridization, Labeling
Related Articles
Isolation:Article Title: The von Hippel-Lindau Tumor Suppressor Gene Inhibits Hepatocyte Growth Factor/Scatter Factor-Induced Invasion and Branching Morphogenesis in Renal Carcinoma Cells Article Snippet: .. The probes included a uPA probe isolated as a 1.5-kb fragment (ATCC 57328), a Article Title: The von Hippel-Lindau Tumor Suppressor Gene Inhibits Hepatocyte Growth Factor/Scatter Factor-Induced Invasion and Branching Morphogenesis in Renal Carcinoma Cells Article Snippet: .. The probes included a uPA probe isolated as a 1.5-kb fragment (ATCC 57328), a Plasmid Preparation:Article Title: Both increased stability and transcription contribute to the induction of the urokinase plasminogen activator receptor (uPAR) message by hypoxia. Article Snippet: Both hypoxia and overexpression of the urokinase plasminogen activator receptor (uPAR) are associated with a poor clinical outcome in human cancers.. Hypoxia has been shown to induce uPAR expression in breast cancer cells and to increase their invasion through Matrigel, a phenomenon which can be blocked using an anti-uPAR antibody.. We examined expression of uPAR mRNA in MCF7 human breast carcinoma cells under hypoxia and found that an increase in the level of the message could be detected at 1% oxygen but was most marked at 0.2 or 0.05% oxygen with an induction of 9to 20-fold over baseline. Article Title: Urokinase Receptor mRNA Stability Involves Tyrosine Phosphorylation in Lung Epithelial Cells Article Snippet: Interaction between urokinase-type plasminogen activator (uPA) and its receptor (uPAR) localizes cellular proteolysis and promotes cellular proliferation and migration, effects that may contribute to the pathogenesis of lung inflammation and neoplasia.. Enhanced uPAR expression as well as stabilization of uPAR mRNA by transforming growth factorand phorbol myristate acetate (PMA) shares a common mechanism involving phosphorylation and dephosphorylation of a uPAR mRNA-binding protein (uPAR mRNABp).. PMA-induced tyrosine phosphorylation of the uPAR mRNABp inhibited the uPAR mRNA–uPAR mRNABp interaction, stabilized uPAR mRNA and enhanced uPAR protein expression. Hybridization:Article Title: Both increased stability and transcription contribute to the induction of the urokinase plasminogen activator receptor (uPAR) message by hypoxia. Article Snippet: Both hypoxia and overexpression of the urokinase plasminogen activator receptor (uPAR) are associated with a poor clinical outcome in human cancers.. Hypoxia has been shown to induce uPAR expression in breast cancer cells and to increase their invasion through Matrigel, a phenomenon which can be blocked using an anti-uPAR antibody.. We examined expression of uPAR mRNA in MCF7 human breast carcinoma cells under hypoxia and found that an increase in the level of the message could be detected at 1% oxygen but was most marked at 0.2 or 0.05% oxygen with an induction of 9to 20-fold over baseline. |