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bluescript sk (1)  (Agilent technologies)


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    Structured Review

    Agilent technologies bluescript sk (1)
    Bluescript Sk (1), supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bluescript+sk+1/pm12606435-46-6-9
    Average 90 stars, based on 1 article reviews
    bluescript sk (1) - by Bioz Stars, 2026-10
    90/100 stars

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    Related Articles

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Activation of class IA PI3K stimulates DNA synthesis in human airway smooth muscle cells
    Article Snippet: Krymskaya, Vera P., Alaina J. Ammit, Rebecca K. Hoffman, Andrew J. Eszterhas, and Reynold A. Panettieri, Jr. Activation of class IA PI3K stimulates DNA synthesis in human airway smooth muscle cells.. Am J Physiol Lung Cell Mol Physiol 280: L1009–L1018, 2001.—The precise mechanisms that regulate increases in airway smooth muscle (ASM) mass in asthma are unknown.. This study determined whether class IA phosphatidylinositol 3-kinase (PI3K) is sufficient to stimulate DNA synthesis and characterized the PI3K isoforms expressed in human ASM cells.

    Sequencing:

    Article Title: Activation of class IA PI3K stimulates DNA synthesis in human airway smooth muscle cells
    Article Snippet: Krymskaya, Vera P., Alaina J. Ammit, Rebecca K. Hoffman, Andrew J. Eszterhas, and Reynold A. Panettieri, Jr. Activation of class IA PI3K stimulates DNA synthesis in human airway smooth muscle cells.. Am J Physiol Lung Cell Mol Physiol 280: L1009–L1018, 2001.—The precise mechanisms that regulate increases in airway smooth muscle (ASM) mass in asthma are unknown.. This study determined whether class IA phosphatidylinositol 3-kinase (PI3K) is sufficient to stimulate DNA synthesis and characterized the PI3K isoforms expressed in human ASM cells.

    Article Title: The Cryptococcus neoformans STE11alpha gene is similar to other fungal mitogen-activated protein kinase kinase kinase (MAPKKK) genes but is mating type specific.
    Article Snippet: .. All routine subcloning procedures were performed in Bluescript SK1 (Stratagene). pC5 is the C. neoformans URA5 gene in Bluescript SK1. pACT contains the C. neoformans actin coding sequence in pCRII (Invitrogen) and was used as a control probe during Northern and Southern analysis. ..

    other:

    Article Title: Drosophila dec-1 eggshell proteins are differentially distributed via a multistep extracellular processing and localization pathway.
    Article Snippet: An ApaI–PstI fragment generated by using mutated 39 and wild-type 59 PCR primers was subcloned into Bluescript SK(1) (Stratagene).

    Article Title: Local high-capacity adenovirus-mediated mCTLA4Ig and mCD40Ig expression prolongs recombinant gene expression in skeletal muscle.
    Article Snippet: To construct the bicistronic vector AdmCTLA4Ig/mCD40Ig, expressing both mCTLA4Ig and mCD40Ig in a ingle vector, a 0.6-kb IRES (internal ribosome entry sequence from enephalomyocarditis virus) was first subcloned into the EcoRI/SmaI site of Bluescript SK(1) (Stratagene, La Jolla, CA), resulting in pSK-IRES.

    Article Title: Native tesmin is a 60-kilodalton protein that undergoes dynamic changes in its localization during spermatogenesis in mice.
    Article Snippet: Restriction fragments were subcloned into the Bluescript SK (1) (Stratagene), and insert DNAs were sequenced from both ends by primer walking.

    Control:

    Article Title: Preproenkephalin mRNA is expressed by C1 and non-C1 barosensitive bulbospinal neurons in the rostral ventrolateral medulla of the rat.
    Article Snippet: The autonomic regions of the thoracolumbar spinal cord receive a dense enkephalinergic (ENK) innervation from supraspinal sources, including the rostral ventrolateral medulla (RVLM).. In the present study, we sought to determine whether the barosensitive bulbospinal (BSBS) neurons of the RVLM express preproenkephalin (PPE) mRNA.. After injection of Fluoro-Gold (FG) into the upper thoracic spinal cord, neurons with PPE mRNA (PPE neurons) were retrogradely labeled throughout the ventrolateral medulla.

    Article Title: The Cryptococcus neoformans STE11alpha gene is similar to other fungal mitogen-activated protein kinase kinase kinase (MAPKKK) genes but is mating type specific.
    Article Snippet: .. All routine subcloning procedures were performed in Bluescript SK1 (Stratagene). pC5 is the C. neoformans URA5 gene in Bluescript SK1. pACT contains the C. neoformans actin coding sequence in pCRII (Invitrogen) and was used as a control probe during Northern and Southern analysis. ..

    Produced:

    Article Title: The Products of theDrosophila stonedLocus Interact with Synaptic Vesicles via Synaptotagmin
    Article Snippet: .. A pGEX-4T-1 clone containing the SpeI–EcoRV fragment was produced by sequential subcloning of this fragment into Bluescript SK 1 (Stratagene, La Jolla, CA), pMal, then via Bluescript SK 1, into the pGEX vector (see Fig. 5A, Construct 1). ..

    Subcloning:

    Article Title: The Products of theDrosophila stonedLocus Interact with Synaptic Vesicles via Synaptotagmin
    Article Snippet: .. A pGEX-4T-1 clone containing the SpeI–EcoRV fragment was produced by sequential subcloning of this fragment into Bluescript SK 1 (Stratagene, La Jolla, CA), pMal, then via Bluescript SK 1, into the pGEX vector (see Fig. 5A, Construct 1). ..

    Article Title: The Cryptococcus neoformans STE11alpha gene is similar to other fungal mitogen-activated protein kinase kinase kinase (MAPKKK) genes but is mating type specific.
    Article Snippet: .. All routine subcloning procedures were performed in Bluescript SK1 (Stratagene). pC5 is the C. neoformans URA5 gene in Bluescript SK1. pACT contains the C. neoformans actin coding sequence in pCRII (Invitrogen) and was used as a control probe during Northern and Southern analysis. ..

    Plasmid Preparation:

    Article Title: The Products of theDrosophila stonedLocus Interact with Synaptic Vesicles via Synaptotagmin
    Article Snippet: .. A pGEX-4T-1 clone containing the SpeI–EcoRV fragment was produced by sequential subcloning of this fragment into Bluescript SK 1 (Stratagene, La Jolla, CA), pMal, then via Bluescript SK 1, into the pGEX vector (see Fig. 5A, Construct 1). ..

    Construct:

    Article Title: The Products of theDrosophila stonedLocus Interact with Synaptic Vesicles via Synaptotagmin
    Article Snippet: .. A pGEX-4T-1 clone containing the SpeI–EcoRV fragment was produced by sequential subcloning of this fragment into Bluescript SK 1 (Stratagene, La Jolla, CA), pMal, then via Bluescript SK 1, into the pGEX vector (see Fig. 5A, Construct 1). ..

    Northern Blot:

    Article Title: The Cryptococcus neoformans STE11alpha gene is similar to other fungal mitogen-activated protein kinase kinase kinase (MAPKKK) genes but is mating type specific.
    Article Snippet: .. All routine subcloning procedures were performed in Bluescript SK1 (Stratagene). pC5 is the C. neoformans URA5 gene in Bluescript SK1. pACT contains the C. neoformans actin coding sequence in pCRII (Invitrogen) and was used as a control probe during Northern and Southern analysis. ..



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