Western Blot:Article Title: CARM1 regulates tubulin autoregulation through PI3KC2α R175 methylation.
Article Snippet: .. The following antibodies were used for Immunoblotting and immunoprecipitation: β-actin (Santa Cruz Biotechnology, Dallas, TX, USA, sc-47778), CARM1 (Bethyl Laboratories, Montgomery, TX, USA, A300-421A), Cyclin B1 (Cell Signaling Technology, Danvers, MA, USA, #12231), GFP (Santa Cruz Biotechnology, sc-9996), GST (Santa Cruz Biotechnology, sc-138), HA (Cell Signaling Technology, #3724), Histone H3 (Cell Signaling Technology, #9715), c-Myc (Santa Cruz Biotechnology, sc-40), PI3KC2α (BD Biosciences, Franklin Lakes, NJ, USA, #611046, Proteintech, Rosemont, IL, USA, 22028–1-AP, and Santa Cruz Biotechnology, sc-365290), Rb (Cell Signaling Technology, #9309), S6 (Cell Signaling Technology, #2217), phospho-S6 (Cell Signaling Technology, #4858), STMN1 (Cell Signaling Technology, #3352), TTC5 (Proteintech, 26112–1-AP), α-tubulin (Santa Cruz Biotechnology, sc-5286 and Cell Signaling Technology, #2144), β-tubulin (Sigma‒Aldrich, T8660), and γ-tubulin (Bethyl Laboratories, A302-631A). ..
Article Title: Reversible arginine methylation of PI3KC2α controls mitotic spindle dynamics
Article Snippet: As described previously [ ], PI3KC2α R175K and R175A mutants were generated using the Muta-Direct Site-Directed Mutagenesis Kit (iNtRON Biotechnology, Seongnam, Korea). .. The following antibodies were used for immunoblotting, immunoprecipitation, or immunostaining: actin (sc-47778; Santa Cruz Biotechnology, Dallas, TX, USA), CARM1 (A300-421 A; Bethyl Laboratories, Montgomery, TX, USA), Cyclin B1 (#12231; Cell Signaling Technology, Danvers, MA, USA), Cyclin D3 (#2936; Cell Signaling Technology), EB1 (#2164; Cell Signaling Technology), GFP (sc-9996; Santa Cruz Biotechnology), HA (#3724; Cell Signaling Technology), Histone H3 (#9715; Cell Signaling Technology), H3K9me2 (A-4035; Epigentek, Farmingdale, NY, USA), H3K9me3 (07-442; Sigma-Aldrich), p-H3S10 (#9701; Cell Signaling Technology), KDM4A (29977-1-AP; Proteintech, Rosemont, IL, USA), c-Myc (sc-40; Santa Cruz Biotechnology), PI3KC2α (#611046: BD Biosciences, Franklin Lakes, NJ, USA; 22028-1-AP: Proteintech; sc-365290: Santa Cruz Biotechnology), p-Ser (sc-81514; Santa Cruz Biotechnology), α-tubulin (sc-5286: Santa Cruz Biotechnology; #2144: Cell Signaling Technology), acetyl-α-tubulin (T7451; Sigma-Aldrich), tyrosinated-α-tubulin (MAB-1864-I; Sigma-Aldrich), and β-tubulin (T8660; Sigma-Aldrich). ..
Article Title: CARM1 regulates tubulin autoregulation through PI3KC2α R175 methylation
Article Snippet: .. The following antibodies were used for Immunoblotting and immunoprecipitation: β-actin (Santa Cruz Biotechnology, Dallas, TX, USA, sc-47778), CARM1 (Bethyl Laboratories, Montgomery, TX, USA, A300-421A), Cyclin B1 (Cell Signaling Technology, Danvers, MA, USA, #12231), GFP (Santa Cruz Biotechnology, sc-9996), GST (Santa Cruz Biotechnology, sc-138), HA (Cell Signaling Technology, #3724), Histone H3 (Cell Signaling Technology, #9715), c-Myc (Santa Cruz Biotechnology, sc-40), PI3KC2α (BD Biosciences, Franklin Lakes, NJ, USA, #611046, Proteintech, Rosemont, IL, USA, 22028–1-AP, and Santa Cruz Biotechnology, sc-365290), Rb (Cell Signaling Technology, #9309), S6 (Cell Signaling Technology, #2217), phospho-S6 (Cell Signaling Technology, #4858), STMN1 (Cell Signaling Technology, #3352), TTC5 (Proteintech, 26112–1-AP), α-tubulin (Santa Cruz Biotechnology, sc-5286 and Cell Signaling Technology, #2144), β-tubulin (Sigma‒Aldrich, T8660), and γ-tubulin (Bethyl Laboratories, A302-631A). ..
Article Title: KDM4A Erases the H3R17me2a Mark, Facilitating Chromosome Condensation
Article Snippet: .. The following antibodies were used for immunoblotting, immunoprecipitation, or immunostaining: actin (Santa Cruz Biotechnology, Dallas, TX, USA, sc‐47778, 1:10000), Aurora B (Abcam, Cambridge, UK, ab2254, 1:5000), Borealin (Novus Biologicals, Centennial, CO, USA, NBP1‐89951, 1:2000), CARM1 (Bethyl Laboratories, Montgomery, TX, USA, A300‐421A, 1:5000), Cyclin B1 (Cell Signaling Technology, Danvers, MA, USA, #12231, 1:2000), Flag (Cell Signaling Technology, #2368 and #8146, 1:5000), HA (Cell Signaling Technology, #3724, 1:5000), histone H3 (Cell Signaling Technology, #9715, 1:5000), H3K9me2 (EpigenTek, Farmingdale, NY, USA, #A‐4035, 1:5000), H3K9me3 (Merck Millipore, Billerica, MA, USA, 07–442, 1:5000 and Diagenode, Denville, NJ, USA, C15200146, 1:500), H3R2me2a (Merck Millipore, 07–585, 1:2000), H3R17me2a (Abcam, ab8284, 1:2000), H3R26me2a (EpigenTek, #A‐3707, 1:2000), H3S10ph (Cell Signaling Technology, #9701, 1:5000), H3T3ph (Abcam, ab78351, 1:5000), INCENP (Thermo Fisher Scientific, 39–2800, 1:2000), KDM1A (Cell Signaling Technology, #2184, 1:5000), KDM3A (Proteintech, Rosemont, IL, USA, 12835‐1‐AP, 1:2000), KDM4A (Proteintech, 29977‐1‐AP, 1:5000), KDM4C (Novus Biologicals, NBP1‐49600, 1:5000), PRMT6 (Bethyl Laboratories, Montgomery, A300‐929A, 1:2000), p‐Ser (Santa Cruz Biotechnology, sc‐81514, 1:1000), p‐Ser PKC substrate (Cell Signaling Technology, #6967, 1:2000), Sp1 (Merck Millipore, CS200631 , 1:5000), Survivin (Abcam, ab76424, 1:2000), Suv39h1 (Cell Signaling Technology, #8729, 1:2000 and Santa Cruz Biotechnology, sc‐23961, 1:500), Suv39h2 (Proteintech, 11338‐1‐AP, 1:1000), and α‐tubulin (Santa Cruz Biotechnology, sc‐5286, 1:500). .. Horseradish peroxidase (HRP)‐conjugated secondary antibodies (111‐035‐003 and 115‐035‐003) were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA, USA).
Article Title: Context-specific applications of CARM1 inhibitors: functional profiles of EZM2302 and TP-064
Article Snippet: GFP-LC3 (#21073) plasmid was purchased from Addgene (Watertown, MA, USA). .. The following antibodies were used for immunoblotting or immunoprecipitation: Actin (Santa Cruz Biotechnology, Dallas, TX, USA, sc-47778), AMPK (Cell Signaling Technology, Danvers, MA, USA, #5832), p-AMPK (Cell Signaling Technology, #2535) CARM1 (Bethyl Laboratories, Montgomery, TX, USA, A300-421 A), DRP1 (BD Biosciences, Franklin Lakes, NJ, USA, #611113), GAPDH (Santa Cruz Biotechnology, sc-25778), histone H3 (Cell Signaling Technology, #9715), H3R2me2a (Sigma-Aldrich, St. Louis, MO, USA, 07–585), H3R17me2a (Abcam, Cambridge, UK, ab8284), H3R26me2a (EpigenTek, Farmingdale, NY, USA, #A-3707), LC3 (Cell Signaling Technology, #12741), p300 (abcam, ab14984), PARP (Santa Cruz Biotechnology, sc-8007), and HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA, USA, #111-035-003, #115-035-003). ..
Article Title: CARM1 phosphorylation at S595 by p38γ MAPK drives ROS-mediated cellular senescence
Article Snippet: .. The following antibodies were used for immunoblotting, immunoprecipitation, or immunostaining: Actin (Santa Cruz Biotechnology, Dallas, TX, USA, sc-47778), CARM1 (Bethyl Laboratories, Montgomery, TX, USA, A300-421A), DRP1 (BD Biosciences, Franklin Lakes, NJ, USA, #611113), ERK (Cell Signaling Technology, #9102), phospho-ERK (Cell Signaling Technology, #9101), GFP (Santa Cruz Biotechnology, sc-9996), HA (Cell Signaling Technology, #3724), histone H3 (Cell Signaling Technology, #9715), HSP70/HSP72 (Enzo Life Sciences, Farmingdale, NY, USA, ADI-SPA-810-F), JNK (Cell Signaling Technology, #9258), phospho-JNK (Cell Signaling Technology, #9251), Mff (ProteinTech, Rosemont, IL, 17090-1-AP), OXPHOS (Abcam, Cambridge, UK, ab110411), p16 (Cell Signaling Technology, #29271), p21 (Cell Signaling Technology, #64016, #2947), p38 MAPK (Cell Signaling Technology, #9212), phospho-p38 MAPK (Cell Signaling Technology, #9211), PARP (Santa Cruz Biotechnology, sc-8007), PGC-1α (Santa Cruz Biotechnology, sc-518025), TFAM (Cell Signaling Technology, #8076; Boster Bio, PB9447), TOM20 (ProteinTech, 11802-1-AP; Abnova, H00009804-M01), VDAC1 (Santa Cruz Biotechnology, sc-390996), Alexa FluorTM-conjugated secondary antibodies (Bethyl Laboratories, A90-116D4, A90-138D2, A120-101D4, A120-101F), and HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA, USA, #111-035-003, #115-035-003). ..
Article Title: Context-specific applications of CARM1 inhibitors: functional profiles of EZM2302 and TP-064.
Article Snippet: GFP-LC3 (#21073) plasmid was purchased from Addgene (Watertown, MA, USA). .. The following antibodies were used for immunoblotting or immunoprecipitation: Actin (Santa Cruz Biotechnology, Dallas, TX, USA, sc-47778), AMPK (Cell Signaling Technology, Danvers, MA, USA, #5832), p-AMPK (Cell Signaling Technology, #2535) CARM1 (Bethyl Laboratories, Montgomery, TX, USA, A300-421 A), DRP1 (BD Biosciences, Franklin Lakes, NJ, USA, #611113), GAPDH (Santa Cruz Biotechnology, sc-25778), histone H3 (Cell Signaling Technology, #9715), H3R2me2a (SigmaAldrich, St. Louis, MO, USA, 07–585), H3R17me2a (Abcam, Cambridge, UK, ab8284), H3R26me2a (EpigenTek, Farmingdale, NY, USA, #A-3707), LC3 (Cell Signaling Technology, #12741), p300 (abcam, ab14984), PARP (Santa Cruz Biotechnology, sc-8007), and HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA, USA, #111-035-003, #115- 035-003). ..
Article Title: Reversible arginine methylation of PI3KC2α controls mitotic spindle dynamics.
Article Snippet: As described previously [12], PI3KC2α R175K and R175A mutants were generated using the Muta-Direct Site-Directed Mutagenesis Kit (iNtRON Biotechnology, Seongnam, Korea). .. The following antibodies were used for immunoblotting, immunoprecipitation, or immunostaining: actin (sc-47778; Santa Cruz Biotechnology, Dallas, TX, USA), CARM1 (A300-421 A; Bethyl Laboratories, Montgomery, TX, USA), Cyclin B1 (#12231; Cell Signaling Technology, Danvers, MA, USA), Cyclin D3 (#2936; Cell Signaling Technology), EB1 (#2164; Cell Signaling Technology), GFP (sc-9996; Santa Cruz Biotechnology), HA (See figure on previous page.) ..
Immunoprecipitation:Article Title: CARM1 regulates tubulin autoregulation through PI3KC2α R175 methylation.
Article Snippet: .. The following antibodies were used for Immunoblotting and immunoprecipitation: β-actin (Santa Cruz Biotechnology, Dallas, TX, USA, sc-47778), CARM1 (Bethyl Laboratories, Montgomery, TX, USA, A300-421A), Cyclin B1 (Cell Signaling Technology, Danvers, MA, USA, #12231), GFP (Santa Cruz Biotechnology, sc-9996), GST (Santa Cruz Biotechnology, sc-138), HA (Cell Signaling Technology, #3724), Histone H3 (Cell Signaling Technology, #9715), c-Myc (Santa Cruz Biotechnology, sc-40), PI3KC2α (BD Biosciences, Franklin Lakes, NJ, USA, #611046, Proteintech, Rosemont, IL, USA, 22028–1-AP, and Santa Cruz Biotechnology, sc-365290), Rb (Cell Signaling Technology, #9309), S6 (Cell Signaling Technology, #2217), phospho-S6 (Cell Signaling Technology, #4858), STMN1 (Cell Signaling Technology, #3352), TTC5 (Proteintech, 26112–1-AP), α-tubulin (Santa Cruz Biotechnology, sc-5286 and Cell Signaling Technology, #2144), β-tubulin (Sigma‒Aldrich, T8660), and γ-tubulin (Bethyl Laboratories, A302-631A). ..
Article Title: Reversible arginine methylation of PI3KC2α controls mitotic spindle dynamics
Article Snippet: As described previously [ ], PI3KC2α R175K and R175A mutants were generated using the Muta-Direct Site-Directed Mutagenesis Kit (iNtRON Biotechnology, Seongnam, Korea). .. The following antibodies were used for immunoblotting, immunoprecipitation, or immunostaining: actin (sc-47778; Santa Cruz Biotechnology, Dallas, TX, USA), CARM1 (A300-421 A; Bethyl Laboratories, Montgomery, TX, USA), Cyclin B1 (#12231; Cell Signaling Technology, Danvers, MA, USA), Cyclin D3 (#2936; Cell Signaling Technology), EB1 (#2164; Cell Signaling Technology), GFP (sc-9996; Santa Cruz Biotechnology), HA (#3724; Cell Signaling Technology), Histone H3 (#9715; Cell Signaling Technology), H3K9me2 (A-4035; Epigentek, Farmingdale, NY, USA), H3K9me3 (07-442; Sigma-Aldrich), p-H3S10 (#9701; Cell Signaling Technology), KDM4A (29977-1-AP; Proteintech, Rosemont, IL, USA), c-Myc (sc-40; Santa Cruz Biotechnology), PI3KC2α (#611046: BD Biosciences, Franklin Lakes, NJ, USA; 22028-1-AP: Proteintech; sc-365290: Santa Cruz Biotechnology), p-Ser (sc-81514; Santa Cruz Biotechnology), α-tubulin (sc-5286: Santa Cruz Biotechnology; #2144: Cell Signaling Technology), acetyl-α-tubulin (T7451; Sigma-Aldrich), tyrosinated-α-tubulin (MAB-1864-I; Sigma-Aldrich), and β-tubulin (T8660; Sigma-Aldrich). ..
Article Title: CARM1 regulates tubulin autoregulation through PI3KC2α R175 methylation
Article Snippet: .. The following antibodies were used for Immunoblotting and immunoprecipitation: β-actin (Santa Cruz Biotechnology, Dallas, TX, USA, sc-47778), CARM1 (Bethyl Laboratories, Montgomery, TX, USA, A300-421A), Cyclin B1 (Cell Signaling Technology, Danvers, MA, USA, #12231), GFP (Santa Cruz Biotechnology, sc-9996), GST (Santa Cruz Biotechnology, sc-138), HA (Cell Signaling Technology, #3724), Histone H3 (Cell Signaling Technology, #9715), c-Myc (Santa Cruz Biotechnology, sc-40), PI3KC2α (BD Biosciences, Franklin Lakes, NJ, USA, #611046, Proteintech, Rosemont, IL, USA, 22028–1-AP, and Santa Cruz Biotechnology, sc-365290), Rb (Cell Signaling Technology, #9309), S6 (Cell Signaling Technology, #2217), phospho-S6 (Cell Signaling Technology, #4858), STMN1 (Cell Signaling Technology, #3352), TTC5 (Proteintech, 26112–1-AP), α-tubulin (Santa Cruz Biotechnology, sc-5286 and Cell Signaling Technology, #2144), β-tubulin (Sigma‒Aldrich, T8660), and γ-tubulin (Bethyl Laboratories, A302-631A). ..
Article Title: KDM4A Erases the H3R17me2a Mark, Facilitating Chromosome Condensation
Article Snippet: .. The following antibodies were used for immunoblotting, immunoprecipitation, or immunostaining: actin (Santa Cruz Biotechnology, Dallas, TX, USA, sc‐47778, 1:10000), Aurora B (Abcam, Cambridge, UK, ab2254, 1:5000), Borealin (Novus Biologicals, Centennial, CO, USA, NBP1‐89951, 1:2000), CARM1 (Bethyl Laboratories, Montgomery, TX, USA, A300‐421A, 1:5000), Cyclin B1 (Cell Signaling Technology, Danvers, MA, USA, #12231, 1:2000), Flag (Cell Signaling Technology, #2368 and #8146, 1:5000), HA (Cell Signaling Technology, #3724, 1:5000), histone H3 (Cell Signaling Technology, #9715, 1:5000), H3K9me2 (EpigenTek, Farmingdale, NY, USA, #A‐4035, 1:5000), H3K9me3 (Merck Millipore, Billerica, MA, USA, 07–442, 1:5000 and Diagenode, Denville, NJ, USA, C15200146, 1:500), H3R2me2a (Merck Millipore, 07–585, 1:2000), H3R17me2a (Abcam, ab8284, 1:2000), H3R26me2a (EpigenTek, #A‐3707, 1:2000), H3S10ph (Cell Signaling Technology, #9701, 1:5000), H3T3ph (Abcam, ab78351, 1:5000), INCENP (Thermo Fisher Scientific, 39–2800, 1:2000), KDM1A (Cell Signaling Technology, #2184, 1:5000), KDM3A (Proteintech, Rosemont, IL, USA, 12835‐1‐AP, 1:2000), KDM4A (Proteintech, 29977‐1‐AP, 1:5000), KDM4C (Novus Biologicals, NBP1‐49600, 1:5000), PRMT6 (Bethyl Laboratories, Montgomery, A300‐929A, 1:2000), p‐Ser (Santa Cruz Biotechnology, sc‐81514, 1:1000), p‐Ser PKC substrate (Cell Signaling Technology, #6967, 1:2000), Sp1 (Merck Millipore, CS200631 , 1:5000), Survivin (Abcam, ab76424, 1:2000), Suv39h1 (Cell Signaling Technology, #8729, 1:2000 and Santa Cruz Biotechnology, sc‐23961, 1:500), Suv39h2 (Proteintech, 11338‐1‐AP, 1:1000), and α‐tubulin (Santa Cruz Biotechnology, sc‐5286, 1:500). .. Horseradish peroxidase (HRP)‐conjugated secondary antibodies (111‐035‐003 and 115‐035‐003) were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA, USA).
Article Title: Context-specific applications of CARM1 inhibitors: functional profiles of EZM2302 and TP-064
Article Snippet: GFP-LC3 (#21073) plasmid was purchased from Addgene (Watertown, MA, USA). .. The following antibodies were used for immunoblotting or immunoprecipitation: Actin (Santa Cruz Biotechnology, Dallas, TX, USA, sc-47778), AMPK (Cell Signaling Technology, Danvers, MA, USA, #5832), p-AMPK (Cell Signaling Technology, #2535) CARM1 (Bethyl Laboratories, Montgomery, TX, USA, A300-421 A), DRP1 (BD Biosciences, Franklin Lakes, NJ, USA, #611113), GAPDH (Santa Cruz Biotechnology, sc-25778), histone H3 (Cell Signaling Technology, #9715), H3R2me2a (Sigma-Aldrich, St. Louis, MO, USA, 07–585), H3R17me2a (Abcam, Cambridge, UK, ab8284), H3R26me2a (EpigenTek, Farmingdale, NY, USA, #A-3707), LC3 (Cell Signaling Technology, #12741), p300 (abcam, ab14984), PARP (Santa Cruz Biotechnology, sc-8007), and HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA, USA, #111-035-003, #115-035-003). ..
Article Title: CARM1 phosphorylation at S595 by p38γ MAPK drives ROS-mediated cellular senescence
Article Snippet: .. The following antibodies were used for immunoblotting, immunoprecipitation, or immunostaining: Actin (Santa Cruz Biotechnology, Dallas, TX, USA, sc-47778), CARM1 (Bethyl Laboratories, Montgomery, TX, USA, A300-421A), DRP1 (BD Biosciences, Franklin Lakes, NJ, USA, #611113), ERK (Cell Signaling Technology, #9102), phospho-ERK (Cell Signaling Technology, #9101), GFP (Santa Cruz Biotechnology, sc-9996), HA (Cell Signaling Technology, #3724), histone H3 (Cell Signaling Technology, #9715), HSP70/HSP72 (Enzo Life Sciences, Farmingdale, NY, USA, ADI-SPA-810-F), JNK (Cell Signaling Technology, #9258), phospho-JNK (Cell Signaling Technology, #9251), Mff (ProteinTech, Rosemont, IL, 17090-1-AP), OXPHOS (Abcam, Cambridge, UK, ab110411), p16 (Cell Signaling Technology, #29271), p21 (Cell Signaling Technology, #64016, #2947), p38 MAPK (Cell Signaling Technology, #9212), phospho-p38 MAPK (Cell Signaling Technology, #9211), PARP (Santa Cruz Biotechnology, sc-8007), PGC-1α (Santa Cruz Biotechnology, sc-518025), TFAM (Cell Signaling Technology, #8076; Boster Bio, PB9447), TOM20 (ProteinTech, 11802-1-AP; Abnova, H00009804-M01), VDAC1 (Santa Cruz Biotechnology, sc-390996), Alexa FluorTM-conjugated secondary antibodies (Bethyl Laboratories, A90-116D4, A90-138D2, A120-101D4, A120-101F), and HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA, USA, #111-035-003, #115-035-003). ..
Article Title: Context-specific applications of CARM1 inhibitors: functional profiles of EZM2302 and TP-064.
Article Snippet: GFP-LC3 (#21073) plasmid was purchased from Addgene (Watertown, MA, USA). .. The following antibodies were used for immunoblotting or immunoprecipitation: Actin (Santa Cruz Biotechnology, Dallas, TX, USA, sc-47778), AMPK (Cell Signaling Technology, Danvers, MA, USA, #5832), p-AMPK (Cell Signaling Technology, #2535) CARM1 (Bethyl Laboratories, Montgomery, TX, USA, A300-421 A), DRP1 (BD Biosciences, Franklin Lakes, NJ, USA, #611113), GAPDH (Santa Cruz Biotechnology, sc-25778), histone H3 (Cell Signaling Technology, #9715), H3R2me2a (SigmaAldrich, St. Louis, MO, USA, 07–585), H3R17me2a (Abcam, Cambridge, UK, ab8284), H3R26me2a (EpigenTek, Farmingdale, NY, USA, #A-3707), LC3 (Cell Signaling Technology, #12741), p300 (abcam, ab14984), PARP (Santa Cruz Biotechnology, sc-8007), and HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA, USA, #111-035-003, #115- 035-003). ..
Article Title: Reversible arginine methylation of PI3KC2α controls mitotic spindle dynamics.
Article Snippet: As described previously [12], PI3KC2α R175K and R175A mutants were generated using the Muta-Direct Site-Directed Mutagenesis Kit (iNtRON Biotechnology, Seongnam, Korea). .. The following antibodies were used for immunoblotting, immunoprecipitation, or immunostaining: actin (sc-47778; Santa Cruz Biotechnology, Dallas, TX, USA), CARM1 (A300-421 A; Bethyl Laboratories, Montgomery, TX, USA), Cyclin B1 (#12231; Cell Signaling Technology, Danvers, MA, USA), Cyclin D3 (#2936; Cell Signaling Technology), EB1 (#2164; Cell Signaling Technology), GFP (sc-9996; Santa Cruz Biotechnology), HA (See figure on previous page.) ..
Immunostaining:Article Title: Reversible arginine methylation of PI3KC2α controls mitotic spindle dynamics
Article Snippet: As described previously [ ], PI3KC2α R175K and R175A mutants were generated using the Muta-Direct Site-Directed Mutagenesis Kit (iNtRON Biotechnology, Seongnam, Korea). .. The following antibodies were used for immunoblotting, immunoprecipitation, or immunostaining: actin (sc-47778; Santa Cruz Biotechnology, Dallas, TX, USA), CARM1 (A300-421 A; Bethyl Laboratories, Montgomery, TX, USA), Cyclin B1 (#12231; Cell Signaling Technology, Danvers, MA, USA), Cyclin D3 (#2936; Cell Signaling Technology), EB1 (#2164; Cell Signaling Technology), GFP (sc-9996; Santa Cruz Biotechnology), HA (#3724; Cell Signaling Technology), Histone H3 (#9715; Cell Signaling Technology), H3K9me2 (A-4035; Epigentek, Farmingdale, NY, USA), H3K9me3 (07-442; Sigma-Aldrich), p-H3S10 (#9701; Cell Signaling Technology), KDM4A (29977-1-AP; Proteintech, Rosemont, IL, USA), c-Myc (sc-40; Santa Cruz Biotechnology), PI3KC2α (#611046: BD Biosciences, Franklin Lakes, NJ, USA; 22028-1-AP: Proteintech; sc-365290: Santa Cruz Biotechnology), p-Ser (sc-81514; Santa Cruz Biotechnology), α-tubulin (sc-5286: Santa Cruz Biotechnology; #2144: Cell Signaling Technology), acetyl-α-tubulin (T7451; Sigma-Aldrich), tyrosinated-α-tubulin (MAB-1864-I; Sigma-Aldrich), and β-tubulin (T8660; Sigma-Aldrich). ..
Article Title: KDM4A Erases the H3R17me2a Mark, Facilitating Chromosome Condensation
Article Snippet: .. The following antibodies were used for immunoblotting, immunoprecipitation, or immunostaining: actin (Santa Cruz Biotechnology, Dallas, TX, USA, sc‐47778, 1:10000), Aurora B (Abcam, Cambridge, UK, ab2254, 1:5000), Borealin (Novus Biologicals, Centennial, CO, USA, NBP1‐89951, 1:2000), CARM1 (Bethyl Laboratories, Montgomery, TX, USA, A300‐421A, 1:5000), Cyclin B1 (Cell Signaling Technology, Danvers, MA, USA, #12231, 1:2000), Flag (Cell Signaling Technology, #2368 and #8146, 1:5000), HA (Cell Signaling Technology, #3724, 1:5000), histone H3 (Cell Signaling Technology, #9715, 1:5000), H3K9me2 (EpigenTek, Farmingdale, NY, USA, #A‐4035, 1:5000), H3K9me3 (Merck Millipore, Billerica, MA, USA, 07–442, 1:5000 and Diagenode, Denville, NJ, USA, C15200146, 1:500), H3R2me2a (Merck Millipore, 07–585, 1:2000), H3R17me2a (Abcam, ab8284, 1:2000), H3R26me2a (EpigenTek, #A‐3707, 1:2000), H3S10ph (Cell Signaling Technology, #9701, 1:5000), H3T3ph (Abcam, ab78351, 1:5000), INCENP (Thermo Fisher Scientific, 39–2800, 1:2000), KDM1A (Cell Signaling Technology, #2184, 1:5000), KDM3A (Proteintech, Rosemont, IL, USA, 12835‐1‐AP, 1:2000), KDM4A (Proteintech, 29977‐1‐AP, 1:5000), KDM4C (Novus Biologicals, NBP1‐49600, 1:5000), PRMT6 (Bethyl Laboratories, Montgomery, A300‐929A, 1:2000), p‐Ser (Santa Cruz Biotechnology, sc‐81514, 1:1000), p‐Ser PKC substrate (Cell Signaling Technology, #6967, 1:2000), Sp1 (Merck Millipore, CS200631 , 1:5000), Survivin (Abcam, ab76424, 1:2000), Suv39h1 (Cell Signaling Technology, #8729, 1:2000 and Santa Cruz Biotechnology, sc‐23961, 1:500), Suv39h2 (Proteintech, 11338‐1‐AP, 1:1000), and α‐tubulin (Santa Cruz Biotechnology, sc‐5286, 1:500). .. Horseradish peroxidase (HRP)‐conjugated secondary antibodies (111‐035‐003 and 115‐035‐003) were purchased from Jackson ImmunoResearch Laboratories (West Grove, PA, USA).
Article Title: CARM1 phosphorylation at S595 by p38γ MAPK drives ROS-mediated cellular senescence
Article Snippet: .. The following antibodies were used for immunoblotting, immunoprecipitation, or immunostaining: Actin (Santa Cruz Biotechnology, Dallas, TX, USA, sc-47778), CARM1 (Bethyl Laboratories, Montgomery, TX, USA, A300-421A), DRP1 (BD Biosciences, Franklin Lakes, NJ, USA, #611113), ERK (Cell Signaling Technology, #9102), phospho-ERK (Cell Signaling Technology, #9101), GFP (Santa Cruz Biotechnology, sc-9996), HA (Cell Signaling Technology, #3724), histone H3 (Cell Signaling Technology, #9715), HSP70/HSP72 (Enzo Life Sciences, Farmingdale, NY, USA, ADI-SPA-810-F), JNK (Cell Signaling Technology, #9258), phospho-JNK (Cell Signaling Technology, #9251), Mff (ProteinTech, Rosemont, IL, 17090-1-AP), OXPHOS (Abcam, Cambridge, UK, ab110411), p16 (Cell Signaling Technology, #29271), p21 (Cell Signaling Technology, #64016, #2947), p38 MAPK (Cell Signaling Technology, #9212), phospho-p38 MAPK (Cell Signaling Technology, #9211), PARP (Santa Cruz Biotechnology, sc-8007), PGC-1α (Santa Cruz Biotechnology, sc-518025), TFAM (Cell Signaling Technology, #8076; Boster Bio, PB9447), TOM20 (ProteinTech, 11802-1-AP; Abnova, H00009804-M01), VDAC1 (Santa Cruz Biotechnology, sc-390996), Alexa FluorTM-conjugated secondary antibodies (Bethyl Laboratories, A90-116D4, A90-138D2, A120-101D4, A120-101F), and HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA, USA, #111-035-003, #115-035-003). ..
Article Title: Reversible arginine methylation of PI3KC2α controls mitotic spindle dynamics.
Article Snippet: As described previously [12], PI3KC2α R175K and R175A mutants were generated using the Muta-Direct Site-Directed Mutagenesis Kit (iNtRON Biotechnology, Seongnam, Korea). .. The following antibodies were used for immunoblotting, immunoprecipitation, or immunostaining: actin (sc-47778; Santa Cruz Biotechnology, Dallas, TX, USA), CARM1 (A300-421 A; Bethyl Laboratories, Montgomery, TX, USA), Cyclin B1 (#12231; Cell Signaling Technology, Danvers, MA, USA), Cyclin D3 (#2936; Cell Signaling Technology), EB1 (#2164; Cell Signaling Technology), GFP (sc-9996; Santa Cruz Biotechnology), HA (See figure on previous page.) ..
Pyrolysis Gas Chromatography:Article Title: CARM1 phosphorylation at S595 by p38γ MAPK drives ROS-mediated cellular senescence
Article Snippet: .. The following antibodies were used for immunoblotting, immunoprecipitation, or immunostaining: Actin (Santa Cruz Biotechnology, Dallas, TX, USA, sc-47778), CARM1 (Bethyl Laboratories, Montgomery, TX, USA, A300-421A), DRP1 (BD Biosciences, Franklin Lakes, NJ, USA, #611113), ERK (Cell Signaling Technology, #9102), phospho-ERK (Cell Signaling Technology, #9101), GFP (Santa Cruz Biotechnology, sc-9996), HA (Cell Signaling Technology, #3724), histone H3 (Cell Signaling Technology, #9715), HSP70/HSP72 (Enzo Life Sciences, Farmingdale, NY, USA, ADI-SPA-810-F), JNK (Cell Signaling Technology, #9258), phospho-JNK (Cell Signaling Technology, #9251), Mff (ProteinTech, Rosemont, IL, 17090-1-AP), OXPHOS (Abcam, Cambridge, UK, ab110411), p16 (Cell Signaling Technology, #29271), p21 (Cell Signaling Technology, #64016, #2947), p38 MAPK (Cell Signaling Technology, #9212), phospho-p38 MAPK (Cell Signaling Technology, #9211), PARP (Santa Cruz Biotechnology, sc-8007), PGC-1α (Santa Cruz Biotechnology, sc-518025), TFAM (Cell Signaling Technology, #8076; Boster Bio, PB9447), TOM20 (ProteinTech, 11802-1-AP; Abnova, H00009804-M01), VDAC1 (Santa Cruz Biotechnology, sc-390996), Alexa FluorTM-conjugated secondary antibodies (Bethyl Laboratories, A90-116D4, A90-138D2, A120-101D4, A120-101F), and HRP-conjugated secondary antibodies (Jackson ImmunoResearch Laboratories, West Grove, PA, USA, #111-035-003, #115-035-003). ..
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