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normal horse serum  (Vector Laboratories)


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    Structured Review

    Vector Laboratories normal horse serum
    Normal Horse Serum, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 452 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blocking+solution/2%2E5%25+Normal+Horse+Serum+Blocking+Solution/pmc13090982-233-10-13
    Average 96 stars, based on 452 article reviews
    normal horse serum - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: AAA ATPase TRIP13 is overexpressed in prostate cancer and promotes tumor progression
    Article Snippet: Subsequently, endogenous peroxidase was blocked by incubating the sections in BLOXALL solution (SP-6000-100, Vector Laboratories, CA, USA) for 10min and washing with TBS. .. Normal horse serum (S-2012-50, Vector Laboratories, CA, USA) was used to block nonspecific binding, and sections were probed with primary antibodies for PCNA, vimentin, and E-cadherin in normal horse serum and incubated for 1 hr at room temperature. .. Slides were then washed in TBS with Tween® 20 (TBS-T) and probed with ImmPRESS horseradish peroxidase anti-rabbit IgG (anti-rabbit, #MP-7401, Vector Laboratories, CA, USA) as a secondary antibody.

    Article Title: Intradiscal Cutibacterium acnes Sustains Modic Type 1‐Like Lesions Over Time in a Rat Lumbar Endplate Injury Model
    Article Snippet: .. Antigens were retrieved for 5 min with antigen‐retrieval buffer (#H3292, Sigma‐Aldrich Inc., St. Louis, MO, USA) followed by 20 min blocking for non‐specific binding with 2.5% normal horse serum (Vector Laboratories Inc., Burlingame, CA, USA). .. Sections were incubated overnight at 4°C in a dark, humidified chamber with a mouse monoclonal primary antibody against rat Substance P (SubP) (1:1000 dilution, #ab14184, Abcam, Cambridge, MA, USA) or rabbit polyclonal primary antibody against rat Glial Fibrillary Acidic Protein (GFAP) (1:2000, #ab7260, Abcam, Cambridge, MA, USA).

    Article Title: AdMSC spheroids encapsulating antioxidant hybrid protein carrier for irradiation-damaged salivary gland repair
    Article Snippet: .. Paraffin-embedded tissue sections (4 μm) were deparaffinized, rehydrated, and subjected to antigen retrieval by boiling in citrate buffer (Sigma-Aldrich, Cat. No. C9999) for 1 h. Slides were cooled to room temperature for 20 min and then placed on ice for another 20 min. Tissue sections were permeabilized using 0.5% Triton X-100 (Sigma-Aldrich, Cat. No. X100) and blocked with 2.5% Normal Horse Serum Blocking Solution (VECTOR, Cat. No. S012) for 30 min. .. Sections were incubated with primary antibodies against AQP5 (1:200, Abcam, ab78486) for acinar cells, α-SMA (1:200, Abcam, ab7817) for myoepithelial cells, MIST1 (1:50, Abcam, ab187978) for acinar cells, SOD2 (1:100, Abcam, ab13534), a ROS scavenging marker, 8-OHdG (1:50, Santa Cruz, sc393871), an oxidized DNA damage marker, NOX4 (1:100, GeneTex, GTX121929), an oxidative stress marker, CD31 (1:40, Novus Biologicals, NB600), an endothelial cell marker for ECs, VEGF-A (1:100, Abcam, ab52917) for angiogenesis marker, CD86 (1:100, Abcam, ab220188) for M1 marker, CD163 (1:200, Abcam, ab182422) for M2 marker, and cleaved caspase-3 (1:100, Cell Signaling Technology, S9661), an apoptosis marker.

    Binding Assay:

    Article Title: AAA ATPase TRIP13 is overexpressed in prostate cancer and promotes tumor progression
    Article Snippet: Subsequently, endogenous peroxidase was blocked by incubating the sections in BLOXALL solution (SP-6000-100, Vector Laboratories, CA, USA) for 10min and washing with TBS. .. Normal horse serum (S-2012-50, Vector Laboratories, CA, USA) was used to block nonspecific binding, and sections were probed with primary antibodies for PCNA, vimentin, and E-cadherin in normal horse serum and incubated for 1 hr at room temperature. .. Slides were then washed in TBS with Tween® 20 (TBS-T) and probed with ImmPRESS horseradish peroxidase anti-rabbit IgG (anti-rabbit, #MP-7401, Vector Laboratories, CA, USA) as a secondary antibody.

    Article Title: Intradiscal Cutibacterium acnes Sustains Modic Type 1‐Like Lesions Over Time in a Rat Lumbar Endplate Injury Model
    Article Snippet: .. Antigens were retrieved for 5 min with antigen‐retrieval buffer (#H3292, Sigma‐Aldrich Inc., St. Louis, MO, USA) followed by 20 min blocking for non‐specific binding with 2.5% normal horse serum (Vector Laboratories Inc., Burlingame, CA, USA). .. Sections were incubated overnight at 4°C in a dark, humidified chamber with a mouse monoclonal primary antibody against rat Substance P (SubP) (1:1000 dilution, #ab14184, Abcam, Cambridge, MA, USA) or rabbit polyclonal primary antibody against rat Glial Fibrillary Acidic Protein (GFAP) (1:2000, #ab7260, Abcam, Cambridge, MA, USA).

    Incubation:

    Article Title: AAA ATPase TRIP13 is overexpressed in prostate cancer and promotes tumor progression
    Article Snippet: Subsequently, endogenous peroxidase was blocked by incubating the sections in BLOXALL solution (SP-6000-100, Vector Laboratories, CA, USA) for 10min and washing with TBS. .. Normal horse serum (S-2012-50, Vector Laboratories, CA, USA) was used to block nonspecific binding, and sections were probed with primary antibodies for PCNA, vimentin, and E-cadherin in normal horse serum and incubated for 1 hr at room temperature. .. Slides were then washed in TBS with Tween® 20 (TBS-T) and probed with ImmPRESS horseradish peroxidase anti-rabbit IgG (anti-rabbit, #MP-7401, Vector Laboratories, CA, USA) as a secondary antibody.

    Article Title: FOXM1 expression is induced by the brain microenvironment and supports CRC brain metastatic adaptation
    Article Snippet: .. Sections were placed in a 3% hydrogen peroxide for 30 min to quench any endogenous peroxidase activity, followed by several washes of PBS with Tween 20 (PBST) and then incubated with 2.5% normal horse serum (ImmPRESS universal polymer kit peroxidase, Vector Laboratories, Newark, CA, USA) for 20 min. Then, the slides were incubated with primary antibodies diluted in antibody diluent (Zytomed Systems, Berlin, Germany) overnight. .. Antibodies used: anti-FOXM1 antibody (sc-376471, Santa Cruz, Dallas, TX, USA, 1:250) and anti-FASN antibody.

    Article Title: Chimeric antigen receptor T cell targeting CD44E variant in HCC holds therapeutic potential
    Article Snippet: .. After antigen retrieval treatment, the slides were blocked with 2.5% normal horse serum (Vector Laboratories, S2012-50) for 2 h and incubated with 60 μL of 2.5% normal horse serum containing primary antibodies for CD44E (10 μg/mL), at 4°C overnight. .. Slides were washed in PBS, treated with Dako REAL Peroxidase-Blocking Solution (Dako, S2023) for 30 min, rinsed, and incubated with 60 μL of Dako REAL Envision HRP Rabbit/Mouse (Dako, K5007) for 1 h. The sections were developed sequentially using Dako REAL EnVision Detection system (Dako, K5007) for 2 min.

    Activity Assay:

    Article Title: FOXM1 expression is induced by the brain microenvironment and supports CRC brain metastatic adaptation
    Article Snippet: .. Sections were placed in a 3% hydrogen peroxide for 30 min to quench any endogenous peroxidase activity, followed by several washes of PBS with Tween 20 (PBST) and then incubated with 2.5% normal horse serum (ImmPRESS universal polymer kit peroxidase, Vector Laboratories, Newark, CA, USA) for 20 min. Then, the slides were incubated with primary antibodies diluted in antibody diluent (Zytomed Systems, Berlin, Germany) overnight. .. Antibodies used: anti-FOXM1 antibody (sc-376471, Santa Cruz, Dallas, TX, USA, 1:250) and anti-FASN antibody.

    Polymer:

    Article Title: FOXM1 expression is induced by the brain microenvironment and supports CRC brain metastatic adaptation
    Article Snippet: .. Sections were placed in a 3% hydrogen peroxide for 30 min to quench any endogenous peroxidase activity, followed by several washes of PBS with Tween 20 (PBST) and then incubated with 2.5% normal horse serum (ImmPRESS universal polymer kit peroxidase, Vector Laboratories, Newark, CA, USA) for 20 min. Then, the slides were incubated with primary antibodies diluted in antibody diluent (Zytomed Systems, Berlin, Germany) overnight. .. Antibodies used: anti-FOXM1 antibody (sc-376471, Santa Cruz, Dallas, TX, USA, 1:250) and anti-FASN antibody.



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