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dapi solution  (Thermo Fisher)


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    Thermo Fisher dapi solution
    Dapi Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blocking+solution+containing+dapi/PBS/pmc12682456-66-34-36
    Average 99 stars, based on 1 article reviews
    dapi solution - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    other:

    Article Title: Derivatives of dolaproine-dolaisoleuine peptides
    Article Snippet: After 72 h of acclimatization, the mice are implanted with SW780 human bladder cancer cells (2×106 cells/mouse), suspended in 50% complete cultrex (Trevigen, Inc.) mixed with PBS (Gibco), and the tumor growth rate is monitored.

    Incubation:

    Article Title: Biomineralized outer membrane vesicles for synergistic immuno-photodynamic therapy of oral squamous cell carcinoma
    Article Snippet: .. First, OMVs containing 1 mg of total protein were dispersed in 1 mL of PBS (Gibco) and incubated overnight in a constant-temperature shaking incubator (200 rpm) at 37 °C to reach equilibrium. ..

    Article Title: Compound design of a patient-derived 3D cell culture system modelling early peritoneal endometriosis
    Article Snippet: Cells were permeabilised with 0.25% (v/v) Triton X-100 (Thermo Fisher Scientific, 85111) in PBS for 10 min at RT and blocked with 2% (w/v) bovine serum albumin (BSA; Sigma-Aldrich, A3803) for 1 h at RT. .. Cells were incubated with primary antibodies overnight at 4°C , followed by three 15 min PBS washes and incubation with secondary antibodies AlexaFluorTM 488 goat-anti-mouse IgG1 (Invitrogen, A21121) and AlexaFluorTM 568 goat-anti-rabbit (Invitrogen, A11011) at 1:1000 dilution, and DAPI (Sigma-Aldrich, D9542) in a 1:500 dilution, for 1 h at RT. .. The slides were mounted using FluoromountTM (Invitrogen, 00-4958-02).

    Article Title: Immunomodulatory topographies regulate myofibroblast differentiation and influence fibrous encapsulation of glaucoma drainage devices
    Article Snippet: Next, the cells were permeabilized with 0.5 % Triton X-100 (Merck) dissolved in PBS for 10 min and blocked with 3 % (w/v) bovine serum albumin (Merck) in PBS for 30 min and then with 5 % (v/v) goat serum (Fisher Scientific) dissolved in PBS for 30 min. Next, cells were incubated with a primary antibody using mouse monoclonal anti α-smooth muscle actin diluted in PBS (1:600; Sigma: A5228) overnight at 4 °C. .. The next day, the cells were washed 3 times with PBS and then incubated with a secondary antibody goat anti-mouse Alexa Flour 488 diluted in PBS (1:500; Molecular probes: A21121) and incubated in the dark for 1 h at room temperature. .. For the α-SMA screen, cells were washed three times with PBS and stained with phalloidin-TRITC diluted in PBS (1:200, ThermoFisher) for 45 min and washed 3 times again with PBS and stained with 4′,6-Diamidino-2-Phenylindole (DAPI) (Sigma Aldrich; 1:500) for 15 min. For the macrophage screen, the cell membrane was stained using CellMaskTM orange (1:1000; ThermoFisher) for 10 min at room temperature.

    Staining:

    Article Title: Immunomodulatory topographies regulate myofibroblast differentiation and influence fibrous encapsulation of glaucoma drainage devices
    Article Snippet: The next day, the cells were washed 3 times with PBS and then incubated with a secondary antibody goat anti-mouse Alexa Flour 488 diluted in PBS (1:500; Molecular probes: A21121) and incubated in the dark for 1 h at room temperature. .. For the α-SMA screen, cells were washed three times with PBS and stained with phalloidin-TRITC diluted in PBS (1:200, ThermoFisher) for 45 min and washed 3 times again with PBS and stained with 4′,6-Diamidino-2-Phenylindole (DAPI) (Sigma Aldrich; 1:500) for 15 min. For the macrophage screen, the cell membrane was stained using CellMaskTM orange (1:1000; ThermoFisher) for 10 min at room temperature. .. Afterward, cells were washed with PBS 3 times and stained with DAPI (Sigma Aldrich; 1:500) for 15 min. After staining, all the surfaces were washed three times with PBS after which they were mounted onto a glass slide using Mowiol (Sigma).

    Membrane:

    Article Title: Immunomodulatory topographies regulate myofibroblast differentiation and influence fibrous encapsulation of glaucoma drainage devices
    Article Snippet: The next day, the cells were washed 3 times with PBS and then incubated with a secondary antibody goat anti-mouse Alexa Flour 488 diluted in PBS (1:500; Molecular probes: A21121) and incubated in the dark for 1 h at room temperature. .. For the α-SMA screen, cells were washed three times with PBS and stained with phalloidin-TRITC diluted in PBS (1:200, ThermoFisher) for 45 min and washed 3 times again with PBS and stained with 4′,6-Diamidino-2-Phenylindole (DAPI) (Sigma Aldrich; 1:500) for 15 min. For the macrophage screen, the cell membrane was stained using CellMaskTM orange (1:1000; ThermoFisher) for 10 min at room temperature. .. Afterward, cells were washed with PBS 3 times and stained with DAPI (Sigma Aldrich; 1:500) for 15 min. After staining, all the surfaces were washed three times with PBS after which they were mounted onto a glass slide using Mowiol (Sigma).

    Sequencing:

    Article Title: Haplotype-resolved genome assemblies of BJ and IMR-90 human fibroblast cell lines reveal extensive structural variation and enable reanalysis of historical sequencing data
    Article Snippet: Both cell lines were authenticated by STR profiling at ATCC ( https://www.atcc.org/ , last accessed 27 February 2026). .. Cells for sequencing were harvested with 0.25% Trypsin–ethylenediaminetetraacetic acid (EDTA) (Thermo Fisher, Cat. No. 25200072), washed with 1× PBS (pH 7.4; Thermo Fisher, Cat. No. 10010023), counted on a CountessTM II FL Automated Cell Counter (Thermo Fisher, Cat. No. AMQAF1000), and used as input for NEB Monarch HMW genomic DNA (gDNA) isolation, Hi-C crosslinking, RNeasy Plus Mini isolation, or frozen as dry pellets and stored at −80°C for use in Oxford Nanopore Technologies (ONT) Ultra-Long (UL) library preparations. .. Estimated cumulative population doubling level (PDL) calculations were performed using PDL = 3.32 (logXe-logXb) + S where log is the base 10 logarithm, Xb is the cell number at the beginning of the incubation period, Xe is the cell number at the end of the incubation period, and S is the starting PDL (ref ATCC Animal Cell Culture Guide ACCG-042024-v08, p6).

    Isolation:

    Article Title: Haplotype-resolved genome assemblies of BJ and IMR-90 human fibroblast cell lines reveal extensive structural variation and enable reanalysis of historical sequencing data
    Article Snippet: Both cell lines were authenticated by STR profiling at ATCC ( https://www.atcc.org/ , last accessed 27 February 2026). .. Cells for sequencing were harvested with 0.25% Trypsin–ethylenediaminetetraacetic acid (EDTA) (Thermo Fisher, Cat. No. 25200072), washed with 1× PBS (pH 7.4; Thermo Fisher, Cat. No. 10010023), counted on a CountessTM II FL Automated Cell Counter (Thermo Fisher, Cat. No. AMQAF1000), and used as input for NEB Monarch HMW genomic DNA (gDNA) isolation, Hi-C crosslinking, RNeasy Plus Mini isolation, or frozen as dry pellets and stored at −80°C for use in Oxford Nanopore Technologies (ONT) Ultra-Long (UL) library preparations. .. Estimated cumulative population doubling level (PDL) calculations were performed using PDL = 3.32 (logXe-logXb) + S where log is the base 10 logarithm, Xb is the cell number at the beginning of the incubation period, Xe is the cell number at the end of the incubation period, and S is the starting PDL (ref ATCC Animal Cell Culture Guide ACCG-042024-v08, p6).

    Hi-C:

    Article Title: Haplotype-resolved genome assemblies of BJ and IMR-90 human fibroblast cell lines reveal extensive structural variation and enable reanalysis of historical sequencing data
    Article Snippet: Both cell lines were authenticated by STR profiling at ATCC ( https://www.atcc.org/ , last accessed 27 February 2026). .. Cells for sequencing were harvested with 0.25% Trypsin–ethylenediaminetetraacetic acid (EDTA) (Thermo Fisher, Cat. No. 25200072), washed with 1× PBS (pH 7.4; Thermo Fisher, Cat. No. 10010023), counted on a CountessTM II FL Automated Cell Counter (Thermo Fisher, Cat. No. AMQAF1000), and used as input for NEB Monarch HMW genomic DNA (gDNA) isolation, Hi-C crosslinking, RNeasy Plus Mini isolation, or frozen as dry pellets and stored at −80°C for use in Oxford Nanopore Technologies (ONT) Ultra-Long (UL) library preparations. .. Estimated cumulative population doubling level (PDL) calculations were performed using PDL = 3.32 (logXe-logXb) + S where log is the base 10 logarithm, Xb is the cell number at the beginning of the incubation period, Xe is the cell number at the end of the incubation period, and S is the starting PDL (ref ATCC Animal Cell Culture Guide ACCG-042024-v08, p6).



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    (A) The primary screen was performed on MCF10A cells in a 384-well format. Genes belonging to a subset of the human genome were knocked down in six replicate plates for 72 h by RNAi along with the listed controls. In a duplicate experiment, cells were stimulated with EGTA. Control wells were distributed on the plates as shown. NT: non-targeting KD – cells were transfected with non-targeting siRNA. No siRNA: cells received RNAiMAX only. Positive control (pos ctrl) – cells were transfected with siRNA against PSENEN. Transfection pos ctrl: cells were transfected with siRNA against polo-like kinase 1 (PLK1) as a cytotoxic indicator of transfection efficiency. Outer wells (yellow) were filled with media to prevent evaporation in experimental wells. (B) To assess endogenous NOTCH1 localization, cells in plates were subjected to automated IF <t>and</t> <t>DAPI/phalloidin</t> labeling to demarcate nuclei and the cell cortex, respectively. The script steps of the image acquisition and analysis developed in-house are as follows: (1) The nuclei were segmented based on DAPI signal. (2) The cell surface was segmented using the phalloidin signal and overlaid on the NOTCH1 channel (3) to quantify levels of cell surface NOTCH1 (4), and the area between the cell cortex and the nuclei was used to determine levels of cytoplasmic NOTCH1 (5). (6) The phalloidin mask was also used to count cells and establish cell-to-cell boundaries. (7) Nuclear size was used as readout of cell viability as compact; pyknotic nuclei identify dead cells. Using such a pipeline, each gene KD was defined by its effects on the amount of NOTCH1 in the cell cortex, in the cytoplasm or in the nucleus.
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    Two-dimensional immunofluorescence stain of isolated bovine muscle satellite cells (BSCs). ( A ) Proliferating bovine satellite stained for <t>DAPI,</t> actin cytoskeleton (Phalloidin), and Pax7, a nuclear marker of satellite cells. Stains show a highly pure satellite cell population, following isolation and pre-plating protocol. ( B ) Following one week of differentiation, cells were stained for DAPI, actin cytoskeleton (Phalloidin), <t>and</t> <t>Troponin</t> T (CT3), a marker of myogenesis. Scale bars are 200 µm.
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    Image Search Results


    (A) The primary screen was performed on MCF10A cells in a 384-well format. Genes belonging to a subset of the human genome were knocked down in six replicate plates for 72 h by RNAi along with the listed controls. In a duplicate experiment, cells were stimulated with EGTA. Control wells were distributed on the plates as shown. NT: non-targeting KD – cells were transfected with non-targeting siRNA. No siRNA: cells received RNAiMAX only. Positive control (pos ctrl) – cells were transfected with siRNA against PSENEN. Transfection pos ctrl: cells were transfected with siRNA against polo-like kinase 1 (PLK1) as a cytotoxic indicator of transfection efficiency. Outer wells (yellow) were filled with media to prevent evaporation in experimental wells. (B) To assess endogenous NOTCH1 localization, cells in plates were subjected to automated IF and DAPI/phalloidin labeling to demarcate nuclei and the cell cortex, respectively. The script steps of the image acquisition and analysis developed in-house are as follows: (1) The nuclei were segmented based on DAPI signal. (2) The cell surface was segmented using the phalloidin signal and overlaid on the NOTCH1 channel (3) to quantify levels of cell surface NOTCH1 (4), and the area between the cell cortex and the nuclei was used to determine levels of cytoplasmic NOTCH1 (5). (6) The phalloidin mask was also used to count cells and establish cell-to-cell boundaries. (7) Nuclear size was used as readout of cell viability as compact; pyknotic nuclei identify dead cells. Using such a pipeline, each gene KD was defined by its effects on the amount of NOTCH1 in the cell cortex, in the cytoplasm or in the nucleus.

    Journal: Life Science Alliance

    Article Title: Novel determinants of NOTCH1 trafficking and signaling in breast epithelial cells

    doi: 10.26508/lsa.202403122

    Figure Lengend Snippet: (A) The primary screen was performed on MCF10A cells in a 384-well format. Genes belonging to a subset of the human genome were knocked down in six replicate plates for 72 h by RNAi along with the listed controls. In a duplicate experiment, cells were stimulated with EGTA. Control wells were distributed on the plates as shown. NT: non-targeting KD – cells were transfected with non-targeting siRNA. No siRNA: cells received RNAiMAX only. Positive control (pos ctrl) – cells were transfected with siRNA against PSENEN. Transfection pos ctrl: cells were transfected with siRNA against polo-like kinase 1 (PLK1) as a cytotoxic indicator of transfection efficiency. Outer wells (yellow) were filled with media to prevent evaporation in experimental wells. (B) To assess endogenous NOTCH1 localization, cells in plates were subjected to automated IF and DAPI/phalloidin labeling to demarcate nuclei and the cell cortex, respectively. The script steps of the image acquisition and analysis developed in-house are as follows: (1) The nuclei were segmented based on DAPI signal. (2) The cell surface was segmented using the phalloidin signal and overlaid on the NOTCH1 channel (3) to quantify levels of cell surface NOTCH1 (4), and the area between the cell cortex and the nuclei was used to determine levels of cytoplasmic NOTCH1 (5). (6) The phalloidin mask was also used to count cells and establish cell-to-cell boundaries. (7) Nuclear size was used as readout of cell viability as compact; pyknotic nuclei identify dead cells. Using such a pipeline, each gene KD was defined by its effects on the amount of NOTCH1 in the cell cortex, in the cytoplasm or in the nucleus.

    Article Snippet: Next, the cells were incubated for 1 h with 1% BSA blocking solution containing DAPI at 1:4,500 (Cat #: D9542; Merck Life Sciences), phalloidin at 1:350 (Cat #: P5282; Merck Life Sciences), and Alexa Fluor 488 anti-rat secondary antibody at 1:400 (Cat #: A21208; Thermo Fisher Scientific).

    Techniques: Control, Transfection, Positive Control, Evaporation, Labeling

    Two-dimensional immunofluorescence stain of isolated bovine muscle satellite cells (BSCs). ( A ) Proliferating bovine satellite stained for DAPI, actin cytoskeleton (Phalloidin), and Pax7, a nuclear marker of satellite cells. Stains show a highly pure satellite cell population, following isolation and pre-plating protocol. ( B ) Following one week of differentiation, cells were stained for DAPI, actin cytoskeleton (Phalloidin), and Troponin T (CT3), a marker of myogenesis. Scale bars are 200 µm.

    Journal: Foods

    Article Title: Extracellular Heme Proteins Influence Bovine Myosatellite Cell Proliferation and the Color of Cell-Based Meat

    doi: 10.3390/foods8100521

    Figure Lengend Snippet: Two-dimensional immunofluorescence stain of isolated bovine muscle satellite cells (BSCs). ( A ) Proliferating bovine satellite stained for DAPI, actin cytoskeleton (Phalloidin), and Pax7, a nuclear marker of satellite cells. Stains show a highly pure satellite cell population, following isolation and pre-plating protocol. ( B ) Following one week of differentiation, cells were stained for DAPI, actin cytoskeleton (Phalloidin), and Troponin T (CT3), a marker of myogenesis. Scale bars are 200 µm.

    Article Snippet: For BAMs, secondary antibodies for Troponin T were diluted in blocking solution containing DAPI (Thermo Fisher, #62248, 1:1000) and added to constructs for 1 h at room temperature.

    Techniques: Immunofluorescence, Staining, Isolation, Marker

    Confocal immunofluorescent imaging of BAMs. BAMs generated from BSC, BSC + Hb, or BSC + Mb (3 mg/mL for both heme proteins) were stained after eight days of differentiation for DAPI, actin cytoskeleton (Phalloidin), and Troponin T (CT3), a marker of myogenesis. Images show multinucleated myotube formation in BSC and BSC + Mb constructs, though not in BSC + Hb constructs. Scale bars are 200 µm.

    Journal: Foods

    Article Title: Extracellular Heme Proteins Influence Bovine Myosatellite Cell Proliferation and the Color of Cell-Based Meat

    doi: 10.3390/foods8100521

    Figure Lengend Snippet: Confocal immunofluorescent imaging of BAMs. BAMs generated from BSC, BSC + Hb, or BSC + Mb (3 mg/mL for both heme proteins) were stained after eight days of differentiation for DAPI, actin cytoskeleton (Phalloidin), and Troponin T (CT3), a marker of myogenesis. Images show multinucleated myotube formation in BSC and BSC + Mb constructs, though not in BSC + Hb constructs. Scale bars are 200 µm.

    Article Snippet: For BAMs, secondary antibodies for Troponin T were diluted in blocking solution containing DAPI (Thermo Fisher, #62248, 1:1000) and added to constructs for 1 h at room temperature.

    Techniques: Imaging, Generated, Staining, Marker, Construct