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Nacalai blocking solution
Blocking Solution, supplied by Nacalai, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blocking+one+p+solution/blocking+solution/pm42289336-77-8-12
Average 86 stars, based on 1 article reviews
blocking solution - by Bioz Stars, 2026-11
86/100 stars

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Article Title: CDKAL1 dysfunction impairs lysine codon translation in podocytes and accelerates chronic kidney disease
Article Snippet: Both cell lines were transduced with lentivirus made by transfecting HEK293FT cells with the sgRNA-containing Lenti-CRISPR v2 Blast, psPAX2 (Addgene), and pMD2.G (Addgene).

Article Title: CDKAL1 dysfunction impairs lysine codon translation in podocytes and accelerates chronic kidney disease.
Article Snippet: Following a 2 h-incubation, the medium in the lower chamber was replaced with RPMI medium supplemented with 40 mg/mL bovine serum albumin (BSA, SigmaAldrich), which had been filtered through a 0.22-μm filter (Millex), and the cells were incubated for an additional 2 h at 37 °C.

Confocal:

Article Title: Spontaneous tauopathy with parkinsonism in an aged cynomolgus macaque
Article Snippet: Antibody binding was visualized using a peroxidase-polymer-based detection system (Histofine Simple Stain MAX-PO kit; Nichirei) with diaminobenzidine as the chromogen. .. For confocal microscopy, sections were similarly processed for antigen retrieval before being incubated in blocking solution (Cat# 06349–64, Nacalai Tesque) for 10 min. .. This was followed by overnight incubation at 4 °C with primary antibodies, and then a 1-h incubation at room temperature with Alexa Fluor Plus 488, 594, or 647-conjugated secondary antibodies (Thermo Fisher Scientific).

Microscopy:

Article Title: Spontaneous tauopathy with parkinsonism in an aged cynomolgus macaque
Article Snippet: Antibody binding was visualized using a peroxidase-polymer-based detection system (Histofine Simple Stain MAX-PO kit; Nichirei) with diaminobenzidine as the chromogen. .. For confocal microscopy, sections were similarly processed for antigen retrieval before being incubated in blocking solution (Cat# 06349–64, Nacalai Tesque) for 10 min. .. This was followed by overnight incubation at 4 °C with primary antibodies, and then a 1-h incubation at room temperature with Alexa Fluor Plus 488, 594, or 647-conjugated secondary antibodies (Thermo Fisher Scientific).

Incubation:

Article Title: Spontaneous tauopathy with parkinsonism in an aged cynomolgus macaque
Article Snippet: Antibody binding was visualized using a peroxidase-polymer-based detection system (Histofine Simple Stain MAX-PO kit; Nichirei) with diaminobenzidine as the chromogen. .. For confocal microscopy, sections were similarly processed for antigen retrieval before being incubated in blocking solution (Cat# 06349–64, Nacalai Tesque) for 10 min. .. This was followed by overnight incubation at 4 °C with primary antibodies, and then a 1-h incubation at room temperature with Alexa Fluor Plus 488, 594, or 647-conjugated secondary antibodies (Thermo Fisher Scientific).

Article Title: Optimum Serum Concentration Enhances Migration of MDA-MB-231 Triple-Negative Breast Cancer Cells and Promotes Intracellular Delivery of Proapoptotic Domain via Cell-Penetrating Peptides
Article Snippet: .. Samples were blocked with blocking solution (Nakalai Tesque) and incubated for 60 min at 25 °C. .. Nuclear and actin staining was performed using 300 nM DAPI and Alexa Fluor 488 Phalloidin (1:200, Invitrogen) in PBS for 20 min at 25 °C, followed by three 20 min rinses with PBS.

Article Title: Role of dynamic changes in human sperm heat shock protein A2 in male infertility.
Article Snippet: .. After washing, the slides were 145 blocked with blocking solution (Blocking One-P; Nacalai Tesque, Inc., Kyoto, Japan) and incubated for 146 1 h at room temperature. ..

Blocking Assay:

Article Title: Spontaneous tauopathy with parkinsonism in an aged cynomolgus macaque
Article Snippet: Antibody binding was visualized using a peroxidase-polymer-based detection system (Histofine Simple Stain MAX-PO kit; Nichirei) with diaminobenzidine as the chromogen. .. For confocal microscopy, sections were similarly processed for antigen retrieval before being incubated in blocking solution (Cat# 06349–64, Nacalai Tesque) for 10 min. .. This was followed by overnight incubation at 4 °C with primary antibodies, and then a 1-h incubation at room temperature with Alexa Fluor Plus 488, 594, or 647-conjugated secondary antibodies (Thermo Fisher Scientific).

Article Title: Optimum Serum Concentration Enhances Migration of MDA-MB-231 Triple-Negative Breast Cancer Cells and Promotes Intracellular Delivery of Proapoptotic Domain via Cell-Penetrating Peptides
Article Snippet: .. Samples were blocked with blocking solution (Nakalai Tesque) and incubated for 60 min at 25 °C. .. Nuclear and actin staining was performed using 300 nM DAPI and Alexa Fluor 488 Phalloidin (1:200, Invitrogen) in PBS for 20 min at 25 °C, followed by three 20 min rinses with PBS.

Article Title: High CD36 Expression Predicts Aggressive Invasion and Recurrence in Oral Squamous Cell Carcinoma
Article Snippet: .. To prevent nonspecific binding of the antibodies, the sections were immersed in a blocking solution (Nacalai Tesque, Kyoto, Japan) for 15 min. .. Subsequently, the sections were incubated with the primary antibody (anti-CD36 rabbit monoclonal antibody, 1:100; clone EPR6573; Abcam, Cambridge, UK) overnight at 4 °C.

Article Title: Selenoprotein P deletion ameliorates metabolic stress-associated anxiety-like behavior in male mice.
Article Snippet: .. RNAscope ISH) After RNAscope ISH, sections were permeabilized with 0.3% Triton X-100 in PBS and treated with a blocking solution (Blocking one histo, Nacalai Tesque, #L2E9350) containing 0.3% Triton X-100 in PBS. .. The sections were incubated overnight at 4 °C with anti-GS (Abcam, Cat# ab228590, rabbit, 1:500, RRID: AB_3665798), anti-Iba1 (Wako, Cat# 019-19741, RRID: AB_839504, rabbit, 1:1000), or NeuN (Cell Signaling Technology Cat# 12943, RRID: AB_2630395) primary antibodies and washed in PBS.

Article Title: Role of dynamic changes in human sperm heat shock protein A2 in male infertility.
Article Snippet: .. After washing, the slides were 145 blocked with blocking solution (Blocking One-P; Nacalai Tesque, Inc., Kyoto, Japan) and incubated for 146 1 h at room temperature. ..

Article Title: CDKAL1 dysfunction impairs lysine codon translation in podocytes and accelerates chronic kidney disease
Article Snippet: O.C.T.Compund , Tissue–Tek , 4583. .. Blocking solution , Nacalai Tasque , 06349-64. .. Dual-Luciferase Reporter Assay System , Promega , E1910.

Binding Assay:

Article Title: High CD36 Expression Predicts Aggressive Invasion and Recurrence in Oral Squamous Cell Carcinoma
Article Snippet: .. To prevent nonspecific binding of the antibodies, the sections were immersed in a blocking solution (Nacalai Tesque, Kyoto, Japan) for 15 min. .. Subsequently, the sections were incubated with the primary antibody (anti-CD36 rabbit monoclonal antibody, 1:100; clone EPR6573; Abcam, Cambridge, UK) overnight at 4 °C.

RNAscope:

Article Title: Selenoprotein P deletion ameliorates metabolic stress-associated anxiety-like behavior in male mice.
Article Snippet: .. RNAscope ISH) After RNAscope ISH, sections were permeabilized with 0.3% Triton X-100 in PBS and treated with a blocking solution (Blocking one histo, Nacalai Tesque, #L2E9350) containing 0.3% Triton X-100 in PBS. .. The sections were incubated overnight at 4 °C with anti-GS (Abcam, Cat# ab228590, rabbit, 1:500, RRID: AB_3665798), anti-Iba1 (Wako, Cat# 019-19741, RRID: AB_839504, rabbit, 1:1000), or NeuN (Cell Signaling Technology Cat# 12943, RRID: AB_2630395) primary antibodies and washed in PBS.

In Situ Hybridization:

Article Title: Selenoprotein P deletion ameliorates metabolic stress-associated anxiety-like behavior in male mice.
Article Snippet: .. RNAscope ISH) After RNAscope ISH, sections were permeabilized with 0.3% Triton X-100 in PBS and treated with a blocking solution (Blocking one histo, Nacalai Tesque, #L2E9350) containing 0.3% Triton X-100 in PBS. .. The sections were incubated overnight at 4 °C with anti-GS (Abcam, Cat# ab228590, rabbit, 1:500, RRID: AB_3665798), anti-Iba1 (Wako, Cat# 019-19741, RRID: AB_839504, rabbit, 1:1000), or NeuN (Cell Signaling Technology Cat# 12943, RRID: AB_2630395) primary antibodies and washed in PBS.



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