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biotinylated goat anti-rabbit igg antibody  (Vector Laboratories)


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    Structured Review

    Vector Laboratories biotinylated goat anti-rabbit igg antibody
    Biotinylated Goat Anti Rabbit Igg Antibody, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 19687 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blocking+buffer+vecstain+elite+abc+kit/Biotinylated+Goat+Anti-Rabbit+IgG+Antibody/custom%40ba-1000%4026045782
    Average 96 stars, based on 19687 article reviews
    biotinylated goat anti-rabbit igg antibody - by Bioz Stars, 2026-10
    96/100 stars

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    Incubation:

    Article Title: Gamma-Oryzanol Solid Dispersion Attenuates Dexamethasone-Induced Depressive-like Behaviors in Rats Associated with Calbindin-1 and Calcium Signaling.
    Article Snippet: Following a rinse with TBS-T, membranes were incubated with primary antibodies forCalb1 (1:1000 dilution, cat #C2724, Sigma-Aldrich, St. Louis, MO, USA) for 4 h at room temperature, followed by overnight incubation at 4 ◦C, and anti-beta-actin (1:1000 dilution, cat #8H10D10, Cell Signaling Technology, Danvers, MA, USA) was incubated overnight at 4 ◦C. .. After three washes, membranes were incubated for 1 h with secondary antibodies: biotinylated goat anti-rabbit IgG (1:200 dilution; Cat# BA-1000, Vector Laboratories, Newark, CA, USA) for Calb1 and goat anti-mouse IgG (1:400 dilution, Cat# A-1000, Vector Laboratories, Newark, CA, USA) for beta-actin. ..

    Article Title: LEAP2 mediates long-term inhibition of orexigenic neurons of the hypothalamus in mice.
    Article Snippet: .. Sections were then incubated with anti-c - Fos antibody (Abcam, Cat# ab214672; RRID: AB_2939046; 1:20,000) for 48 h at 4 °C, followed by a biotinylated anti-r abbit antibody (Vector Laboratories, Cat# BA-1000; 1:3,000; RRID: AB_2313606) for 1 h at room temperature and an avidin–peroxidase complex (Vectastain Elite ABC kit) according to the manufacturer’s instructions. ..

    Article Title: Exposure to an environmentally relevant phthalate mixture impairs ovarian endothelial cell function in vitro.
    Article Snippet: .. Slides were incubated for 1.5 hr at room temperature with a recombinant rabbit primary antibody for Ki-67 (1:600 dilution; Fisher Scientific; Waltham, MA), rinsed with PBS, then incubated for 30 min with a goat anti-rabbit IgG biotinylated antibody (1.5 mg/ml; Vector Laboratories; Newark, CA). .. Color was developed using the Vectastain ABC kit (Vector Laboratories; Newark, CA).

    Transcranial Magnetic Stimulation:

    Article Title: Buffaloed in Brandenburg: Germany’s first brush with foot-and-mouth disease after four decades of freedom
    Article Snippet: .. The primary antibody (in-house polyclonal rabbit anti-FMDV serum, “MKS-3ABC-3D”) was applied for 1 hour at RT (1:1000; diluted in TBS), the secondary biotinylated goat anti-rabbit antibody (BA-1000-1.5, Vector Laboratories, USA; RRID: AB_2313606; 1:200 diluted in TBS) was applied for 30 min at RT. .. The color was developed by incubating the slides with ABC solution (Vectastain Elite ABC kit; Vector Laboratories), followed by exposure to 3-amino-9-ethylcarbazole substrate (Dako, USA).

    Avidin-Biotin Assay:

    Article Title: LEAP2 mediates long-term inhibition of orexigenic neurons of the hypothalamus in mice.
    Article Snippet: .. Sections were then incubated with anti-c - Fos antibody (Abcam, Cat# ab214672; RRID: AB_2939046; 1:20,000) for 48 h at 4 °C, followed by a biotinylated anti-r abbit antibody (Vector Laboratories, Cat# BA-1000; 1:3,000; RRID: AB_2313606) for 1 h at room temperature and an avidin–peroxidase complex (Vectastain Elite ABC kit) according to the manufacturer’s instructions. ..

    Sequencing:

    Article Title: Apc-deficient pituitary stem cells drive adamantinomatous craniopharyngioma through senescence-associated inflammation.
    Article Snippet: .. Reagents and tools table Reagent/resource Reference or source Identifier or catalogue number Experimental models Rosa26CAGLoxpSTOPLoxpTdTomato Jax-Lab stock #007905 ApcLoxp(Exon15)/Loxp(Exon15) Jax-Lab stock #029275 © The Author(s) EMBO Molecular Medicine 15 Reagent/resource Reference or source Identifier or catalogue number ApcLoxp(Exon1-15)/Loxp(Exon1-15) Jax-Lab stock #009045 Tcf/Lef-1:H2B-GFP Jax-Lab stock #032838 Sox2CreERT2/+ Jax-Lab stock #017593 Ctnnb1LoxP(exon3)/+ M Taketo (Harada, N. et al, 1999) Provided by M Taketo (Harada, N. et al, 1999) Antibodies Rabbit αphosphorylated-histone H3 (α-pHH3) 1:500 dilution, Millipore, 06-570 Millipore 06-570 Rabbit α-PRL 1:500 dilution The National Hormone and Peptide Program (NHPP), Harbour-UCLA Medical Centre Rabbit α-LH 1:500 dilution The National Hormone and Peptide Program (NHPP), Harbour-UCLA Medical Centre Rabbit α-TSH 1:500 dilution The National Hormone and Peptide Program (NHPP), Harbour-UCLA Medical Centre Rabbit α-GH 1:500 dilution The National Hormone and Peptide Program (NHPP), Harbour-UCLA Medical Centre Rabbit α-Pomc 1:500 dilution The National Hormone and Peptide Program (NHPP), Harbour-UCLA Medical Centre Rabbit α-GSU 1:500 dilution The National Hormone and Peptide Program (NHPP), Harbour-UCLA Medical Centre Goat α-Sox2 1:200 dilution, Statech, GT15098 Statech, GT15098 Rabbit α-Sox9 1:500 dilution Millipore (AB5535) Mouse α-β-Catenin 1:300 dilution, SIGMA, C7082 SIGMA (C7082) Chicken α-GFP 1:100 Abcam (ab13970) Abcam (ab13970) Mouse α-APC CT (clone C-APC 28.9) 1:1000 Millipore (MAB3786) Millipore (MAB3786) Rabbit α-p21 [EPR18021] 1:1000 (Abcam) ab188224 Abcam (ab188224) Rabbit α-pSTAT3 1:300 Cell Signalling Cell Signalling (9145) Rabbit α-NF-kB (p65) 1:300 (Abcam) ab16502 Abcam (ab16502) Rabbit α-β-Catenin 1:300 (Abcam) Abcam (ab16051) Rat α-CDKN2A/ p16INK4a antibody [PABLO33B]) 1:300 (Abcam) ab241543 Abcam (ab241543) Mouse α-H2A.X phosphor (Ser 139) 1:150 Biolegend G13402 Biolegend (G13402) Reagent/resource Reference or source Identifier or catalogue number Mouse α-β-Actin 1:5000 Merck A5441 Merck (A5441) Rabbit α-Vinculin 1:1000 dilution Abcam (ab129002) Biotinylated goat antirabbit antibody 1:300 dilution, Vector Laboratories, BA-1000 Vector Laboratories (BA-1000) Biotinylated horse antigoat antibody 1:200 dilution, Vector Laboratories, BA-9500 Vector Laboratories (BA-9500) Donkey anti-goat antibody, Alexa Fluor 568 1:300, Invitrogen, A-11057 Invitrogen (A11057) Goat anti-mouse antibody, Alexa Fluor 488 1:300, Invitrogen, A-11001 Invitrogen (A11001) Goat-anti-mouse IRDye 800CW (1:1000) 926-32210 LI-COR 926-32210 LICOR Goat-anti-Rabbit IRDye 680RD (1:1000) 926-68071 LI-COR 926-68071 LICOR Oligonucleotides and other sequence-based reagents Apc (exon 15) FW: ACGAAGACCACAGGCAAATC REV: CCACAAAGTTCCACATGCATTAC Merck Axin2 FW: ACGAAGACCACAGGCAAATC REV: CCACAAAGTTCCACATGCATTAC Merck Lef1 FW: ACTGTCAGGCGACACTTCCATG REV: GTGCTCCTGTTTGACCTGAGGT Merck Wnt7a FW: TTCGCCAAGGTCTTCGTGGATG REV: TACAGGAGCCTGACACACCATG Merck Ccl2 FW: TTAAAAACCTGGATCGGAACCAA REV: GTCTGGACCCATTCCTTCTTGG Merck Fgf3 FW: GCAAGCTCTACTGCGCTACCAA REV: CACTTCCACCGCAGTAATCTCC Merck Ccl3 FW: TGTACCATGACACTCTGCAAC REV: CAACGATGAATTGGCGTGGAA Merck Notum FW: CGTGGTACACTCAAGGATGTGC REV: GCCTTATGGCTGTCATGGAAGC Merck Gh FW: TCAGAATGCCCAGGCTGCTTTC REV: ACTGGATGAGCAGCAGCGAGAA Merck Tsh FW: GAGAGTGTGCCTACTGCCTG REV: ACAGACATCCTGAGAGAGTGC Merck Prl FW: CTCAGGCCATCTTGGAGAAG REV: TCGGAGAGAAGTCTGGCAGT Merck p21 (Cdkn1a) FW: GTCGCTGTCTTGCACTCTGG RE: CTCTTGCAGAAGACCAATCTGC Merck Software R version 4.3.0 (2023- 04-21) https://www.r-project.org NA 16 EMBO Molecular Medicine © The Author(s) Reagent/resource Reference or source Identifier or catalogue number GraphPad PRISM 11.0.1 (99) GraphPad PRISM Photoshop 2025 Adobe Other .. The transgenic lines Rosa26CAGLoxpSTOPLoxpTdTomato (stock #007905) (Madisen et al, 2010), ApcLoxp(Exon15)/Loxp(Exon15) (stock #029275) (RobanusMaandag et al, 2010), ApcLoxp(Exon1-15)/Loxp(Exon1-15) (stock #009045) (Cheung et al, 2010), Tcf/Lef-1:H2B-GFP (stock #032838) (FerrerVaquer et al, 2010) and the Sox2CreERT2/+ (stock #017593) (Arnold et al, 2011) were obtained from the JAX-lab and have been previously described.

    Recombinant:

    Article Title: Exposure to an environmentally relevant phthalate mixture impairs ovarian endothelial cell function in vitro.
    Article Snippet: .. Slides were incubated for 1.5 hr at room temperature with a recombinant rabbit primary antibody for Ki-67 (1:600 dilution; Fisher Scientific; Waltham, MA), rinsed with PBS, then incubated for 30 min with a goat anti-rabbit IgG biotinylated antibody (1.5 mg/ml; Vector Laboratories; Newark, CA). .. Color was developed using the Vectastain ABC kit (Vector Laboratories; Newark, CA).

    Blocking Assay:

    Article Title: 5-HT 2A receptors in layer 5 pyramidal neurons mediate the neuroplastic but not the hallucinogenic effects of psilocybin.
    Article Snippet: It is unclear how serotonin 2A receptors (5-HT2ARs) in cortical layer 5 pyramidal neurons differentially contribute to psilocybin-induced hallucinations versus neuroplasticity.. Here we show that psilocybin promotes synapse formation and maturation while accelerating the elimination of pre-existing synapses.. Cell type-specific manipulation further demonstrated that 5-HT2AR signaling in cortical layer 5 pyramidal neurons is necessary and sufficient for psilocybin-induced synaptic remodeling but dispensable for the head-twitch response, a rodent proxy of hallucination.

    Next-Generation Sequencing:

    Article Title: 5-HT 2A receptors in layer 5 pyramidal neurons mediate the neuroplastic but not the hallucinogenic effects of psilocybin.
    Article Snippet: It is unclear how serotonin 2A receptors (5-HT2ARs) in cortical layer 5 pyramidal neurons differentially contribute to psilocybin-induced hallucinations versus neuroplasticity.. Here we show that psilocybin promotes synapse formation and maturation while accelerating the elimination of pre-existing synapses.. Cell type-specific manipulation further demonstrated that 5-HT2AR signaling in cortical layer 5 pyramidal neurons is necessary and sufficient for psilocybin-induced synaptic remodeling but dispensable for the head-twitch response, a rodent proxy of hallucination.

    Staining:

    Article Title: 5-HT 2A receptors in layer 5 pyramidal neurons mediate the neuroplastic but not the hallucinogenic effects of psilocybin.
    Article Snippet: It is unclear how serotonin 2A receptors (5-HT2ARs) in cortical layer 5 pyramidal neurons differentially contribute to psilocybin-induced hallucinations versus neuroplasticity.. Here we show that psilocybin promotes synapse formation and maturation while accelerating the elimination of pre-existing synapses.. Cell type-specific manipulation further demonstrated that 5-HT2AR signaling in cortical layer 5 pyramidal neurons is necessary and sufficient for psilocybin-induced synaptic remodeling but dispensable for the head-twitch response, a rodent proxy of hallucination.

    Cell-Signaling:

    Article Title: TFEB drives ER stress-induced apoptosis and limits cyst growth in polycystic kidney disease
    Article Snippet: .. We used the following antibodies: TFEB (Bethyl, A303-673A, for mouse samples; Cell Signaling Technology, 37785, for human samples), LRP2 (BiCell Scientific, 31012), UMOD (R&D Systems, AF5175), AQP2 (Santa Cruz, sc-28629), GPRC5A (Novus Biologicals; NBP1-89743), YAP (Cell Signaling Technology, 14074), CHOP (Proteintech, 15204–1-AP), Ki67 (Abcam, 16667), GADD34 (Abcam, 9869), S6RP (Cell Signaling Technology, 2217), phospho-S6RP (Ser235/236; Cell Signaling Technology, 2211), TMSB4X (Proteintech, 19850–1-AP), Clusterin alpha (Abcam, ab184100), biotinylated secondary antibodies (Vector Laboratories, BA1000, BA-2001, and BA-4000), and Alexa Fluor–conjugated secondary antibodies (Thermo Fisher Scientific, A11015, A21247, and A31572). ..



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