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KAC Co Ltd blocking agent block ace
Blocking Agent Block Ace, supplied by KAC Co Ltd, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blocking+agent/blocking+agent+block+ace/pm36562292-78-25-29
Average 90 stars, based on 1 article reviews
blocking agent block ace - by Bioz Stars, 2026-10
90/100 stars

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Incubation:

Article Title: Association between Hepatic Oxidative Stress Related Factors and Activation of Wnt/β-Catenin Signaling in NAFLD-Induced Hepatocellular Carcinoma
Article Snippet: The slides were deparaffinized and incubated with BOND Novocastra Epitope Retrieval Solution pH 6 (Leica Biosystems, Nussloch, Germany) for 20 min at 100 °C for Heat-Induced Epitope Retrieval. .. After incubation with a 3% hydrogen peroxide solution for 5 min to inactivate the endogenous peroxidase, samples were treated with a blocking agent (Block Ace; KAC Co., Ltd., Kyoto, Japan) for 10 min to prevent a non-specific reaction. .. Samples were reacted with the anti-Glutamine synthetase antibody (Merck Millipore, Billerica, MA, USA, 1:500 dilution) as the primary antibody for 15 min. After that, the reaction with secondary antibody and color development was performed with BOND Polymer Refine Detection (Leica Biosystems, Nussloch, Germany).

Blocking Assay:

Article Title: Association between Hepatic Oxidative Stress Related Factors and Activation of Wnt/β-Catenin Signaling in NAFLD-Induced Hepatocellular Carcinoma
Article Snippet: The slides were deparaffinized and incubated with BOND Novocastra Epitope Retrieval Solution pH 6 (Leica Biosystems, Nussloch, Germany) for 20 min at 100 °C for Heat-Induced Epitope Retrieval. .. After incubation with a 3% hydrogen peroxide solution for 5 min to inactivate the endogenous peroxidase, samples were treated with a blocking agent (Block Ace; KAC Co., Ltd., Kyoto, Japan) for 10 min to prevent a non-specific reaction. .. Samples were reacted with the anti-Glutamine synthetase antibody (Merck Millipore, Billerica, MA, USA, 1:500 dilution) as the primary antibody for 15 min. After that, the reaction with secondary antibody and color development was performed with BOND Polymer Refine Detection (Leica Biosystems, Nussloch, Germany).

Article Title: The G protein‐coupled receptor ligand apelin‐13 ameliorates skeletal muscle atrophy induced by chronic kidney disease
Article Snippet: .. A 4 μm‐thick section of the frozen tibialis anterior muscle was obtained; mounting compound was washed with phosphate‐buffered saline (PBS) and then blocked with 4% blocking agent (block ace, KAC Co., Ltd., Hyogo, Japan) in PBS at room temperature (RT) for 30 min. After that, samples were reacted with a primary antibody (anti‐laminin) at RT for 60 min. After that, samples were reacted with secondary antibody (anti‐rabbit‐IgG‐Alexa546) at RT for 60 min in a dark condition. .. Samples were observed by a microscope (BZ‐X700 microscope, Keyence, Osaka, Japan).

Article Title: The G protein-coupled receptor ligand apelin-13 ameliorates skeletal muscle atrophy induced by chronic kidney disease.
Article Snippet: .. A 4 μm-thick section of the frozen tibialis anterior muscle was obtained; mounting compound was washed with phosphate-buffered saline (PBS) and then blocked with 4% blocking agent (block ace, KAC Co., Ltd., Hyogo, Japan) in PBS at room temperature (RT) for 30 min. After that, samples were reacted with a primary antibody (anti-laminin) at RT for 60 min. After that, samples were reacted with secondary antibody (anti-rabbit-IgG-Alexa546) at RT for 60 min in a dark condition. .. Samples were observed by a microscope (BZ-X700 microscope, Keyence, Osaka, Japan).

Saline:

Article Title: The G protein‐coupled receptor ligand apelin‐13 ameliorates skeletal muscle atrophy induced by chronic kidney disease
Article Snippet: .. A 4 μm‐thick section of the frozen tibialis anterior muscle was obtained; mounting compound was washed with phosphate‐buffered saline (PBS) and then blocked with 4% blocking agent (block ace, KAC Co., Ltd., Hyogo, Japan) in PBS at room temperature (RT) for 30 min. After that, samples were reacted with a primary antibody (anti‐laminin) at RT for 60 min. After that, samples were reacted with secondary antibody (anti‐rabbit‐IgG‐Alexa546) at RT for 60 min in a dark condition. .. Samples were observed by a microscope (BZ‐X700 microscope, Keyence, Osaka, Japan).

Article Title: The G protein-coupled receptor ligand apelin-13 ameliorates skeletal muscle atrophy induced by chronic kidney disease.
Article Snippet: .. A 4 μm-thick section of the frozen tibialis anterior muscle was obtained; mounting compound was washed with phosphate-buffered saline (PBS) and then blocked with 4% blocking agent (block ace, KAC Co., Ltd., Hyogo, Japan) in PBS at room temperature (RT) for 30 min. After that, samples were reacted with a primary antibody (anti-laminin) at RT for 60 min. After that, samples were reacted with secondary antibody (anti-rabbit-IgG-Alexa546) at RT for 60 min in a dark condition. .. Samples were observed by a microscope (BZ-X700 microscope, Keyence, Osaka, Japan).



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