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bleomycin sulfate  (Thermo Fisher)


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    Structured Review

    Thermo Fisher bleomycin sulfate
    Bleomycin Sulfate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bleomycin/Bleomycin+sulfate/pm42338185-162-0-2
    Average 94 stars, based on 1 article reviews
    bleomycin sulfate - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Nociceptor neurons suppress alveolar macrophage-induced Siglec-F + neutrophil-mediated inflammation to protect against pulmonary fibrosis.
    Article Snippet: In brief Sensory innervation impacts several lung diseases, but whether it influences pulmonary fibrosis (PF) is unclear.. Hiroki et al. report that depletion of TRPV1 nociceptors leads to worsening of inflammation-induced PF.. Mechanistically, nociceptor depletion results in aberrant production of the neuropeptide VIP by alveolar macrophages, which increases TGF-β1 levels and causes Siglec-F neutrophils to release NETs that contribute to PF pathology.

    Article Title: Substrate Stiffness and Viscoelasticity Influence Fibroblast Senescence.
    Article Snippet: IMR- 90 primary lung fibroblasts (ATCC) were grown in Gibco Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10 v/v% fetal bovine serum (FBS) and 1 v/v% antibioticantimycotic (1000 U/mL penicillin, 1000 μg/mL streptomycin, and 0.25 μg/mL amphotericin B) and used between passage 2 and 6 for all experiments. .. Bleomycin sulfate (ThermoFisher) was used to induce senescence by adding 10 mg/mL stock directly to the cell culture medium while cells were in flasks to achieve the desired final concentration. ..

    Mouse Assay:

    Article Title: Nociceptor neurons suppress alveolar macrophage-induced Siglec-F + neutrophil-mediated inflammation to protect against pulmonary fibrosis.
    Article Snippet: In brief Sensory innervation impacts several lung diseases, but whether it influences pulmonary fibrosis (PF) is unclear.. Hiroki et al. report that depletion of TRPV1 nociceptors leads to worsening of inflammation-induced PF.. Mechanistically, nociceptor depletion results in aberrant production of the neuropeptide VIP by alveolar macrophages, which increases TGF-β1 levels and causes Siglec-F neutrophils to release NETs that contribute to PF pathology.

    Injection:

    Article Title: Nociceptor neurons suppress alveolar macrophage-induced Siglec-F + neutrophil-mediated inflammation to protect against pulmonary fibrosis.
    Article Snippet: In brief Sensory innervation impacts several lung diseases, but whether it influences pulmonary fibrosis (PF) is unclear.. Hiroki et al. report that depletion of TRPV1 nociceptors leads to worsening of inflammation-induced PF.. Mechanistically, nociceptor depletion results in aberrant production of the neuropeptide VIP by alveolar macrophages, which increases TGF-β1 levels and causes Siglec-F neutrophils to release NETs that contribute to PF pathology.

    Staining:

    Article Title: Inhalable TFRC-Targeted Extracellular Vesicles Delivery of siTGF-β1 Alleviates Pulmonary Fibrosis via Dual Inhibition of Ferroptosis and Fibroblast Activation
    Article Snippet: Six- to eight-week-old male C57BL/6J mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China) and maintained at a constant temperature (25 ± 1 °C). .. DMEM culture medium, penicillin-streptomycin (PS) and Fetal bovine serum (Gibco), Bleomycin sulfate, Fer-1 and DFO (MCE), HE staining kit, Masson’s staining kit and 0.5% crystal violet staining solution (Yeasen), EPPB enhanced Prussian blue staining kit and FITC-Phalloidin (Solarbio), Transwell (Corning), CCK-8 and 4% PFA (Biosharp), C11-BODIPY581/591 (Merck), RhoNox-1, Deferoxamine (DFO), Bleomycin sulfate and Liproxstatin-1 (MCE), Dihydroethidium (Beyotime), BC-T4 culture medium (Baso), anti-CD34, anti-CD19 and anti-CD45 (BD), anti-CD90, anti-CD73 and anti-CD105 (Biolegend), anti-CD63, anti-Flotillin-1, anti-Calnexin and anti-TGF-β1 (HUABIO), anti-Collagen I (Abcam), anti-TFRC (Abways), anti-α-SMA (Proteintech), Recombinant Mouse/Rat TGF-beta 1 (Novoprotein, Shanghai, China). ..

    CCK-8 Assay:

    Article Title: Inhalable TFRC-Targeted Extracellular Vesicles Delivery of siTGF-β1 Alleviates Pulmonary Fibrosis via Dual Inhibition of Ferroptosis and Fibroblast Activation
    Article Snippet: Six- to eight-week-old male C57BL/6J mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China) and maintained at a constant temperature (25 ± 1 °C). .. DMEM culture medium, penicillin-streptomycin (PS) and Fetal bovine serum (Gibco), Bleomycin sulfate, Fer-1 and DFO (MCE), HE staining kit, Masson’s staining kit and 0.5% crystal violet staining solution (Yeasen), EPPB enhanced Prussian blue staining kit and FITC-Phalloidin (Solarbio), Transwell (Corning), CCK-8 and 4% PFA (Biosharp), C11-BODIPY581/591 (Merck), RhoNox-1, Deferoxamine (DFO), Bleomycin sulfate and Liproxstatin-1 (MCE), Dihydroethidium (Beyotime), BC-T4 culture medium (Baso), anti-CD34, anti-CD19 and anti-CD45 (BD), anti-CD90, anti-CD73 and anti-CD105 (Biolegend), anti-CD63, anti-Flotillin-1, anti-Calnexin and anti-TGF-β1 (HUABIO), anti-Collagen I (Abcam), anti-TFRC (Abways), anti-α-SMA (Proteintech), Recombinant Mouse/Rat TGF-beta 1 (Novoprotein, Shanghai, China). ..

    Recombinant:

    Article Title: Inhalable TFRC-Targeted Extracellular Vesicles Delivery of siTGF-β1 Alleviates Pulmonary Fibrosis via Dual Inhibition of Ferroptosis and Fibroblast Activation
    Article Snippet: Six- to eight-week-old male C57BL/6J mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China) and maintained at a constant temperature (25 ± 1 °C). .. DMEM culture medium, penicillin-streptomycin (PS) and Fetal bovine serum (Gibco), Bleomycin sulfate, Fer-1 and DFO (MCE), HE staining kit, Masson’s staining kit and 0.5% crystal violet staining solution (Yeasen), EPPB enhanced Prussian blue staining kit and FITC-Phalloidin (Solarbio), Transwell (Corning), CCK-8 and 4% PFA (Biosharp), C11-BODIPY581/591 (Merck), RhoNox-1, Deferoxamine (DFO), Bleomycin sulfate and Liproxstatin-1 (MCE), Dihydroethidium (Beyotime), BC-T4 culture medium (Baso), anti-CD34, anti-CD19 and anti-CD45 (BD), anti-CD90, anti-CD73 and anti-CD105 (Biolegend), anti-CD63, anti-Flotillin-1, anti-Calnexin and anti-TGF-β1 (HUABIO), anti-Collagen I (Abcam), anti-TFRC (Abways), anti-α-SMA (Proteintech), Recombinant Mouse/Rat TGF-beta 1 (Novoprotein, Shanghai, China). ..

    Cell Culture:

    Article Title: Substrate Stiffness and Viscoelasticity Influence Fibroblast Senescence.
    Article Snippet: IMR- 90 primary lung fibroblasts (ATCC) were grown in Gibco Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10 v/v% fetal bovine serum (FBS) and 1 v/v% antibioticantimycotic (1000 U/mL penicillin, 1000 μg/mL streptomycin, and 0.25 μg/mL amphotericin B) and used between passage 2 and 6 for all experiments. .. Bleomycin sulfate (ThermoFisher) was used to induce senescence by adding 10 mg/mL stock directly to the cell culture medium while cells were in flasks to achieve the desired final concentration. ..

    Article Title: ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition Zone
    Article Snippet: .. Depending on a specific strain, cells were cultured with or without the addition of 20 μg/ml paromomycin (Sigma-Aldrich), 15 μg/ml bleomycin (Invitrogen), or both antibiotics with 10 μg/ml paromomycin and 5 μg/ml bleomycin. ..

    Stable Transfection:

    Article Title: O -GlcNAcylation of XRCC4 controls its stability and confers resistance to DNA double-strand break damage in cancer cells
    Article Snippet: .. HeLa cell lines, stably integrated copy of the artificial recombination substrate DR-GFP with an I-SceI site (HR) or EJ5-GFP with two I-SceI sites (NHEJ), were transfected with I-Sce1 (Addgene, #26477) using Lipofectamine 2000 (Invitrogen) and incubated with 10 μg/mL bleomycin for 48 h. GFP expression was obtained by Fluorescence Activated Cell Sorting (FACS). ..

    Non-Homologous End Joining:

    Article Title: O -GlcNAcylation of XRCC4 controls its stability and confers resistance to DNA double-strand break damage in cancer cells
    Article Snippet: .. HeLa cell lines, stably integrated copy of the artificial recombination substrate DR-GFP with an I-SceI site (HR) or EJ5-GFP with two I-SceI sites (NHEJ), were transfected with I-Sce1 (Addgene, #26477) using Lipofectamine 2000 (Invitrogen) and incubated with 10 μg/mL bleomycin for 48 h. GFP expression was obtained by Fluorescence Activated Cell Sorting (FACS). ..

    Transfection:

    Article Title: O -GlcNAcylation of XRCC4 controls its stability and confers resistance to DNA double-strand break damage in cancer cells
    Article Snippet: .. HeLa cell lines, stably integrated copy of the artificial recombination substrate DR-GFP with an I-SceI site (HR) or EJ5-GFP with two I-SceI sites (NHEJ), were transfected with I-Sce1 (Addgene, #26477) using Lipofectamine 2000 (Invitrogen) and incubated with 10 μg/mL bleomycin for 48 h. GFP expression was obtained by Fluorescence Activated Cell Sorting (FACS). ..

    Incubation:

    Article Title: O -GlcNAcylation of XRCC4 controls its stability and confers resistance to DNA double-strand break damage in cancer cells
    Article Snippet: .. HeLa cell lines, stably integrated copy of the artificial recombination substrate DR-GFP with an I-SceI site (HR) or EJ5-GFP with two I-SceI sites (NHEJ), were transfected with I-Sce1 (Addgene, #26477) using Lipofectamine 2000 (Invitrogen) and incubated with 10 μg/mL bleomycin for 48 h. GFP expression was obtained by Fluorescence Activated Cell Sorting (FACS). ..

    Expressing:

    Article Title: O -GlcNAcylation of XRCC4 controls its stability and confers resistance to DNA double-strand break damage in cancer cells
    Article Snippet: .. HeLa cell lines, stably integrated copy of the artificial recombination substrate DR-GFP with an I-SceI site (HR) or EJ5-GFP with two I-SceI sites (NHEJ), were transfected with I-Sce1 (Addgene, #26477) using Lipofectamine 2000 (Invitrogen) and incubated with 10 μg/mL bleomycin for 48 h. GFP expression was obtained by Fluorescence Activated Cell Sorting (FACS). ..

    Fluorescence:

    Article Title: O -GlcNAcylation of XRCC4 controls its stability and confers resistance to DNA double-strand break damage in cancer cells
    Article Snippet: .. HeLa cell lines, stably integrated copy of the artificial recombination substrate DR-GFP with an I-SceI site (HR) or EJ5-GFP with two I-SceI sites (NHEJ), were transfected with I-Sce1 (Addgene, #26477) using Lipofectamine 2000 (Invitrogen) and incubated with 10 μg/mL bleomycin for 48 h. GFP expression was obtained by Fluorescence Activated Cell Sorting (FACS). ..

    FACS:

    Article Title: O -GlcNAcylation of XRCC4 controls its stability and confers resistance to DNA double-strand break damage in cancer cells
    Article Snippet: .. HeLa cell lines, stably integrated copy of the artificial recombination substrate DR-GFP with an I-SceI site (HR) or EJ5-GFP with two I-SceI sites (NHEJ), were transfected with I-Sce1 (Addgene, #26477) using Lipofectamine 2000 (Invitrogen) and incubated with 10 μg/mL bleomycin for 48 h. GFP expression was obtained by Fluorescence Activated Cell Sorting (FACS). ..

    Plasmid Preparation:

    Article Title: The Role of IgG Subclass in Antibody-Mediated Protection against Carbapenem-Resistant Klebsiella pneumoniae
    Article Snippet: .. The plasmid was transformed into MMC39 by electroporation and plated onto Lennox (low-salt) LB with pH adjusted to 8.0 and containing 50 g/ml bleomycin (Zeocin; Thermo Fisher). ..

    Transformation Assay:

    Article Title: The Role of IgG Subclass in Antibody-Mediated Protection against Carbapenem-Resistant Klebsiella pneumoniae
    Article Snippet: .. The plasmid was transformed into MMC39 by electroporation and plated onto Lennox (low-salt) LB with pH adjusted to 8.0 and containing 50 g/ml bleomycin (Zeocin; Thermo Fisher). ..

    Electroporation:

    Article Title: The Role of IgG Subclass in Antibody-Mediated Protection against Carbapenem-Resistant Klebsiella pneumoniae
    Article Snippet: .. The plasmid was transformed into MMC39 by electroporation and plated onto Lennox (low-salt) LB with pH adjusted to 8.0 and containing 50 g/ml bleomycin (Zeocin; Thermo Fisher). ..



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