lentief1 flag p2a blast (Addgene inc)
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lentief1 flag p2a blast
Lentief1 Flag P2a Blast, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blast++sequence+alignment+software+package/Lenti%E2%80%90Cas9%E2%80%902A%E2%80%90Blast+(Plasmid+%2373310)/pmc11647120-351-9-19
Average 95 stars, based on 99 article reviews
Lentief1 Flag P2a Blast, supplied by Addgene inc, used in various techniques. Bioz Stars score: 95/100, based on 99 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blast++sequence+alignment+software+package/Lenti%E2%80%90Cas9%E2%80%902A%E2%80%90Blast+(Plasmid+%2373310)/pmc11647120-351-9-19
Average 95 stars, based on 99 article reviews
lentief1 flag p2a blast - by Bioz Stars,
2026-10
95/100 stars
Images
1) Product Images from "Increased CSN5 expression enhances the sensitivity to lenalidomide in multiple myeloma cells"
Article Title: Increased CSN5 expression enhances the sensitivity to lenalidomide in multiple myeloma cells
Journal: iScience
doi: 10.1016/j.isci.2024.111399
Figure Legend Snippet: Overexpression of CSN5 induces LEN sensitivity (A) Induction of LEN sensitivity in RPMI8226, KMS11, KMS12BM, and MM.1S cells overexpressing FLAG-CSN5. Empty vector (lentiEF1-FLAG-P2A-Blast) was used as a control. We used 100 μM and 1 μM LEN for LEN-resistant and -sensitive cells, respectively. Data represent the mean ± standard deviation ( n = 5). Student's t test. (B) Relationship between LEN sensitivity and the endogenous CSN5 expression levels in the 15 cell lines. Correlations between protein levels and cell viability were evaluated using simple linear regression in GraphPad Prism 8. Statistical significance was set at p < 0.05. (C) Western blots representing the expression of CUL4A and CSN5 in RPMI8226 cells overexpressing WT FLAG-CSN5 or FLAG-CSN5-H138Q. β-actin was used as a loading control. The larger proportion of neddylated CUL4A suggested that CSN5-H138Q decreased deneddylation activity. (D) LEN sensitivity in RPMI8226 cells overexpressing FLAG-CSN5-H138Q. The lentiEF1-FLAG-P2A-Blast vector harboring FLAG-tagged CSN5 or FLAG-tagged CSN5 with H138Q mutation was used for lentiviral infection with RPMI8226. We used 100 μM LEN in this assay. Data represent the mean ± standard deviation ( n = 5). Student's t test. (E) LEN response pathway (CRBN axis) involved in the antitumor mechanism of LEN. (F) Western blots of IKZF1, IKFZ3, IRF4, CSN5 expression levels after treatment with the indicated dose of LEN in FLAG-CSN5- or empty vector-overexpressing RPMI8226 cells. β-Αctin was used as the loading control. The protein bands in western blotting of IKZF1, IKZF3 and IRF4 were quantified using iBright Analysis Software. Band densities of IKZF1, IKZF3 and IRF4 were normalized to that of β-actin. Data represent the mean ( n = 2).
Techniques Used: Over Expression, Plasmid Preparation, Control, Standard Deviation, Expressing, Western Blot, Activity Assay, Mutagenesis, Infection, Software
Figure Legend Snippet:
Techniques Used: Recombinant, Sequencing, Modification, Bicinchoninic Acid Protein Assay, Mass Spectrometry, Western Blot, Software
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