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plasmid pwzl blast gfp  (Addgene inc)


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    Structured Review

    Addgene inc plasmid pwzl blast gfp
    KEY RESOURCES TABLE
    Plasmid Pwzl Blast Gfp, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 33 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blast++sequence+alignment+software+package/pWZL+Blast+GFP+(Plasmid+%2312269)/pmc06499081-116-0-12
    Average 93 stars, based on 33 article reviews
    plasmid pwzl blast gfp - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "METTL13 methylation of eEF1A increases translational output to promote tumorigenesis"

    Article Title: METTL13 methylation of eEF1A increases translational output to promote tumorigenesis

    Journal: Cell

    doi: 10.1016/j.cell.2018.11.038

    KEY RESOURCES TABLE
    Figure Legend Snippet: KEY RESOURCES TABLE

    Techniques Used: Virus, Plasmid Preparation, Recombinant, Protease Inhibitor, Sequencing, Modification, Bicinchoninic Acid Protein Assay, Activity Assay, Enzyme-linked Immunosorbent Assay, Staining, Silver Staining, Mutagenesis, Control, Software, Membrane

    Related Articles

    Expressing:

    Article Title: Targeting WNK1 in blood cancers
    Article Snippet: .. For lentivirus production, 293FT cells were co-transfected with sgRNA expressing lentiviral vectors (see above) and psPAX2 lentiviral plasmid (Addgene #12269) and pMD2.G plasmid (Addgene #12259) using the calcium phosphate transfection method. .. 48-72 hours after transfection viral supernatants were harvested and spun onto RetroNectin® (TaKaRa: T202) coated 6 well plates for 2 hours at 2000 g. After spinning the plates were washed briefly in PBS and cells were spun onto the plates at 400 g for 5 min.

    Article Title: Novel role of zinc-finger protein 518 in heterochromatin formation on α-satellite DNA
    Article Snippet: .. To construct the retroviral expression vector (pDEST253), tetR cassettes containing the TetR-YFP-encoding region and Gateway reading frame cassette were PCR-amplified from pDEST152JTY and inserted into pWZL-Blast-GFP between Xho I and Sal I sites using the In-Fusion HD Cloning Kit. pWZL-Blast-GFP was obtained from Addgene, plasmid #12269. .. To overexpress the target proteins in human or Escherichia coli cells, plasmids were generated by DNA recombination between the entry plasmids mentioned above and pDEST131NGFP (for GFP fusion proteins), pDEST152JTY (for TetR-YFP fusion proteins), pDEST155Halo (for Halo-tagged proteins), pDEST253JTY (for TetR-YFP fusion proteins using retrovirus infection), pDEST174HATMBP (for HAT [KDHLIHNVHKEEHAHNK]-MBP fusion proteins) or pDEST172HGST (for His 6 -GST fusion proteins) using Gateway LR Clonase II Enzyme mix (#11 791 020, Thermo Fisher Scientific) ( , ).

    Plasmid Preparation:

    Article Title: Targeting WNK1 in blood cancers
    Article Snippet: .. For lentivirus production, 293FT cells were co-transfected with sgRNA expressing lentiviral vectors (see above) and psPAX2 lentiviral plasmid (Addgene #12269) and pMD2.G plasmid (Addgene #12259) using the calcium phosphate transfection method. .. 48-72 hours after transfection viral supernatants were harvested and spun onto RetroNectin® (TaKaRa: T202) coated 6 well plates for 2 hours at 2000 g. After spinning the plates were washed briefly in PBS and cells were spun onto the plates at 400 g for 5 min.

    Article Title: Chlamydia trachomatis impairs T cell priming by inducing dendritic cell death.
    Article Snippet: To target two genes simultaneously, sgRNA nucleotides were cloned into the C-CHIME plasmid vector pXPR_219 (Addgene ID# 164559, kindly provided by Arlene Sharpe) using BsmBI (New England Biosciences) followed by BfuAI (New England Biosciences) restriction digestion. .. Lentiviral constructs were generated by transfecting 293T cells (ATCC) grown in DMEM (Corning) supplemented with 10% FBS with pMD2.G (Addgene #12259), psPAX2 (Addgene #12269), and the edited pXPR_219 plasmid. ..

    Article Title: BRD9 inhibition overcomes oncolytic virus therapy resistance in glioblastoma.
    Article Snippet: CRISPR-mediated pooled library lentivirus production The CRISPR Mouse Knockout Pooled Library (Brie) (Addgene, #73632), which comprises 78,637 sgRNAs targeting 19,674 genes, was utilized for the generation of lentivirus. .. Cotransfection of the library plasmid, psPAX plasmid (Addgene, #12269), and pMD2. .. G plasmid (Addgene, #12259) was carried out in HEK293T cells using polyethyleneimine transfection reagent (1 mg/mL; Polyscience, #23966-1) following the manufacturer’s protocol.

    Article Title: Chlamydia trachomatis impairs T cell priming by inducing dendritic cell death
    Article Snippet: To target two genes simultaneously, sgRNA nucleotides were cloned into the C-CHIME plasmid vector pXPR_219 (Addgene ID# 164559, kindly provided by Arlene Sharpe) using BsmBI (New England Biosciences) followed by BfuAI (New England Biosciences) restriction digestion. .. Lentiviral constructs were generated by transfecting 293T cells (ATCC) grown in DMEM (Corning) supplemented with 10% FBS with pMD2.G (Addgene #12259), psPAX2 (Addgene #12269), and the edited pXPR_219 plasmid. ..

    Article Title: Novel role of zinc-finger protein 518 in heterochromatin formation on α-satellite DNA
    Article Snippet: .. To construct the retroviral expression vector (pDEST253), tetR cassettes containing the TetR-YFP-encoding region and Gateway reading frame cassette were PCR-amplified from pDEST152JTY and inserted into pWZL-Blast-GFP between Xho I and Sal I sites using the In-Fusion HD Cloning Kit. pWZL-Blast-GFP was obtained from Addgene, plasmid #12269. .. To overexpress the target proteins in human or Escherichia coli cells, plasmids were generated by DNA recombination between the entry plasmids mentioned above and pDEST131NGFP (for GFP fusion proteins), pDEST152JTY (for TetR-YFP fusion proteins), pDEST155Halo (for Halo-tagged proteins), pDEST253JTY (for TetR-YFP fusion proteins using retrovirus infection), pDEST174HATMBP (for HAT [KDHLIHNVHKEEHAHNK]-MBP fusion proteins) or pDEST172HGST (for His 6 -GST fusion proteins) using Gateway LR Clonase II Enzyme mix (#11 791 020, Thermo Fisher Scientific) ( , ).

    Transfection:

    Article Title: Targeting WNK1 in blood cancers
    Article Snippet: .. For lentivirus production, 293FT cells were co-transfected with sgRNA expressing lentiviral vectors (see above) and psPAX2 lentiviral plasmid (Addgene #12269) and pMD2.G plasmid (Addgene #12259) using the calcium phosphate transfection method. .. 48-72 hours after transfection viral supernatants were harvested and spun onto RetroNectin® (TaKaRa: T202) coated 6 well plates for 2 hours at 2000 g. After spinning the plates were washed briefly in PBS and cells were spun onto the plates at 400 g for 5 min.

    Construct:

    Article Title: Chlamydia trachomatis impairs T cell priming by inducing dendritic cell death.
    Article Snippet: To target two genes simultaneously, sgRNA nucleotides were cloned into the C-CHIME plasmid vector pXPR_219 (Addgene ID# 164559, kindly provided by Arlene Sharpe) using BsmBI (New England Biosciences) followed by BfuAI (New England Biosciences) restriction digestion. .. Lentiviral constructs were generated by transfecting 293T cells (ATCC) grown in DMEM (Corning) supplemented with 10% FBS with pMD2.G (Addgene #12259), psPAX2 (Addgene #12269), and the edited pXPR_219 plasmid. ..

    Article Title: Chlamydia trachomatis impairs T cell priming by inducing dendritic cell death
    Article Snippet: To target two genes simultaneously, sgRNA nucleotides were cloned into the C-CHIME plasmid vector pXPR_219 (Addgene ID# 164559, kindly provided by Arlene Sharpe) using BsmBI (New England Biosciences) followed by BfuAI (New England Biosciences) restriction digestion. .. Lentiviral constructs were generated by transfecting 293T cells (ATCC) grown in DMEM (Corning) supplemented with 10% FBS with pMD2.G (Addgene #12259), psPAX2 (Addgene #12269), and the edited pXPR_219 plasmid. ..

    Article Title: Novel role of zinc-finger protein 518 in heterochromatin formation on α-satellite DNA
    Article Snippet: .. To construct the retroviral expression vector (pDEST253), tetR cassettes containing the TetR-YFP-encoding region and Gateway reading frame cassette were PCR-amplified from pDEST152JTY and inserted into pWZL-Blast-GFP between Xho I and Sal I sites using the In-Fusion HD Cloning Kit. pWZL-Blast-GFP was obtained from Addgene, plasmid #12269. .. To overexpress the target proteins in human or Escherichia coli cells, plasmids were generated by DNA recombination between the entry plasmids mentioned above and pDEST131NGFP (for GFP fusion proteins), pDEST152JTY (for TetR-YFP fusion proteins), pDEST155Halo (for Halo-tagged proteins), pDEST253JTY (for TetR-YFP fusion proteins using retrovirus infection), pDEST174HATMBP (for HAT [KDHLIHNVHKEEHAHNK]-MBP fusion proteins) or pDEST172HGST (for His 6 -GST fusion proteins) using Gateway LR Clonase II Enzyme mix (#11 791 020, Thermo Fisher Scientific) ( , ).

    Generated:

    Article Title: Chlamydia trachomatis impairs T cell priming by inducing dendritic cell death.
    Article Snippet: To target two genes simultaneously, sgRNA nucleotides were cloned into the C-CHIME plasmid vector pXPR_219 (Addgene ID# 164559, kindly provided by Arlene Sharpe) using BsmBI (New England Biosciences) followed by BfuAI (New England Biosciences) restriction digestion. .. Lentiviral constructs were generated by transfecting 293T cells (ATCC) grown in DMEM (Corning) supplemented with 10% FBS with pMD2.G (Addgene #12259), psPAX2 (Addgene #12269), and the edited pXPR_219 plasmid. ..

    Article Title: Chlamydia trachomatis impairs T cell priming by inducing dendritic cell death
    Article Snippet: To target two genes simultaneously, sgRNA nucleotides were cloned into the C-CHIME plasmid vector pXPR_219 (Addgene ID# 164559, kindly provided by Arlene Sharpe) using BsmBI (New England Biosciences) followed by BfuAI (New England Biosciences) restriction digestion. .. Lentiviral constructs were generated by transfecting 293T cells (ATCC) grown in DMEM (Corning) supplemented with 10% FBS with pMD2.G (Addgene #12259), psPAX2 (Addgene #12269), and the edited pXPR_219 plasmid. ..

    other:

    Article Title: BRD9 inhibition overcomes oncolytic virus therapy resistance in glioblastoma
    Article Snippet: The anti-mouse CD8 antibody (Bioxcell, #BE0223) was used for CD8 + T cell depletion in vivo and diluted in PBS before intraperitoneal injection at the concentration of 8 mg/kg on day 2, 6, and 10.

    Cotransfection:

    Article Title: BRD9 inhibition overcomes oncolytic virus therapy resistance in glioblastoma.
    Article Snippet: CRISPR-mediated pooled library lentivirus production The CRISPR Mouse Knockout Pooled Library (Brie) (Addgene, #73632), which comprises 78,637 sgRNAs targeting 19,674 genes, was utilized for the generation of lentivirus. .. Cotransfection of the library plasmid, psPAX plasmid (Addgene, #12269), and pMD2. .. G plasmid (Addgene, #12259) was carried out in HEK293T cells using polyethyleneimine transfection reagent (1 mg/mL; Polyscience, #23966-1) following the manufacturer’s protocol.

    Retroviral:

    Article Title: Novel role of zinc-finger protein 518 in heterochromatin formation on α-satellite DNA
    Article Snippet: .. To construct the retroviral expression vector (pDEST253), tetR cassettes containing the TetR-YFP-encoding region and Gateway reading frame cassette were PCR-amplified from pDEST152JTY and inserted into pWZL-Blast-GFP between Xho I and Sal I sites using the In-Fusion HD Cloning Kit. pWZL-Blast-GFP was obtained from Addgene, plasmid #12269. .. To overexpress the target proteins in human or Escherichia coli cells, plasmids were generated by DNA recombination between the entry plasmids mentioned above and pDEST131NGFP (for GFP fusion proteins), pDEST152JTY (for TetR-YFP fusion proteins), pDEST155Halo (for Halo-tagged proteins), pDEST253JTY (for TetR-YFP fusion proteins using retrovirus infection), pDEST174HATMBP (for HAT [KDHLIHNVHKEEHAHNK]-MBP fusion proteins) or pDEST172HGST (for His 6 -GST fusion proteins) using Gateway LR Clonase II Enzyme mix (#11 791 020, Thermo Fisher Scientific) ( , ).

    Polymerase Chain Reaction:

    Article Title: Novel role of zinc-finger protein 518 in heterochromatin formation on α-satellite DNA
    Article Snippet: .. To construct the retroviral expression vector (pDEST253), tetR cassettes containing the TetR-YFP-encoding region and Gateway reading frame cassette were PCR-amplified from pDEST152JTY and inserted into pWZL-Blast-GFP between Xho I and Sal I sites using the In-Fusion HD Cloning Kit. pWZL-Blast-GFP was obtained from Addgene, plasmid #12269. .. To overexpress the target proteins in human or Escherichia coli cells, plasmids were generated by DNA recombination between the entry plasmids mentioned above and pDEST131NGFP (for GFP fusion proteins), pDEST152JTY (for TetR-YFP fusion proteins), pDEST155Halo (for Halo-tagged proteins), pDEST253JTY (for TetR-YFP fusion proteins using retrovirus infection), pDEST174HATMBP (for HAT [KDHLIHNVHKEEHAHNK]-MBP fusion proteins) or pDEST172HGST (for His 6 -GST fusion proteins) using Gateway LR Clonase II Enzyme mix (#11 791 020, Thermo Fisher Scientific) ( , ).

    Cloning:

    Article Title: Novel role of zinc-finger protein 518 in heterochromatin formation on α-satellite DNA
    Article Snippet: .. To construct the retroviral expression vector (pDEST253), tetR cassettes containing the TetR-YFP-encoding region and Gateway reading frame cassette were PCR-amplified from pDEST152JTY and inserted into pWZL-Blast-GFP between Xho I and Sal I sites using the In-Fusion HD Cloning Kit. pWZL-Blast-GFP was obtained from Addgene, plasmid #12269. .. To overexpress the target proteins in human or Escherichia coli cells, plasmids were generated by DNA recombination between the entry plasmids mentioned above and pDEST131NGFP (for GFP fusion proteins), pDEST152JTY (for TetR-YFP fusion proteins), pDEST155Halo (for Halo-tagged proteins), pDEST253JTY (for TetR-YFP fusion proteins using retrovirus infection), pDEST174HATMBP (for HAT [KDHLIHNVHKEEHAHNK]-MBP fusion proteins) or pDEST172HGST (for His 6 -GST fusion proteins) using Gateway LR Clonase II Enzyme mix (#11 791 020, Thermo Fisher Scientific) ( , ).



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