spcas9 blastr pcbhcas9 blastr addgene (Addgene inc)

Addgene inc is a verified supplier

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Addgene inc spcas9 blastr pcbhcas9 blastr addgene
Spcas9 Blastr Pcbhcas9 Blastr Addgene, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/blast++sequence+alignment+software+package/spCas9-BlastR+(pCBhCas9-BlastR)+(Plasmid+%2371489)/pm40513559-753-211-213
Average 93 stars, based on 11 article reviews

spcas9 blastr pcbhcas9 blastr addgene - by Bioz Stars, 2026-10

93/100 stars

Images

other:

Article Title: Human airway submucosal gland organoids to study respiratory inflammation and infection.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER A83-01 Tocris Cat#2939 SB202190 Sigma-Aldrich Cat# S7067 N-Acetyl-L-cysteine Sigma-Aldrich Cat#A9165 Nicotinamide (1M) Sigma-Aldrich Cat#N0636 Lenti-XTM Concentrator Takara Bio Cat#631232 Recombinant RNasin® RNase Inhibitor, 10,000 U Promega Cat#N2515 GoScriptTM Reverse Transcriptase kit Promega Cat#A5000 Oligo(dT) 15 Primer, 500ug/ml 20ug Promega Cat#C1101 Random Primer, 500ug/ml 20ug Promega Cat#C1181 BbsI-HF NEB Cat#R3539S Y-27632 dihydrochloride Abmole Bioscience Cat#M1817 iQTM SYBR® Green Supermix BioRad Cat#1708887 DAPI, 4 ′ ,6-Diamidine-2 ′ -phenylindole dihydrochloride Sigma-Aldrich Cat#10236276001 DRAQ5 Biostatus Cat#DR05500 TRIzol Reagent Thermo Fisher Scientific Cat#15596026 Tissue Freezing Medium Leica Biosystems Cat#14020108926 VECTASHIELD® Antifade Mounting Medium VectorLabs Cat#H-1000-10 Nitrocellulose Membrane Bio-Rad Cat#1620115 Critical Commercial Assays NucleoSpin RNA, Mini kit for RNA purification MACHEREY-NAGEL Cat#740955.50 Miniprep DNA isolation kit Thermo Fisher Scientific Cat#K210003 NucleoBond Xtra Midi Plus kit for transfection-grade plasmid DNA MACHEREY-NAGEL Cat#740412.50 Quick-DNA Microprep Kit Zymo Research Cat#D3021 Lenti-XTM qRT-PCR Titration Kit Takara Bio Cat#631235 Amersham ECL Prime Western Blotting Detection Reagent Cytiva Cat#RPN2232 Deposited Data scRNA-seq profiling of human SMG and SAE organoids This paper GEO: GSE271084 Bulk RNA-seq profiling of human SMG organoids upon cytokine treatments This paper GEO: GSE271148 Bulk RNA-seq profiling of human SMG organoids upon virus infection This paper GEO: GSE271264 Processed human airway single-cell atlas Goldfarbmuren et al. 21 GEO: GSE134174 Recombinant DNA sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR) Addgene Cat#71485 spCas9-BlastR (pCBhCas9-BlastR) Addgene Cat#71489 Software and Algorithms FlowJo software (v.10.4) FlowJo https://www.flowjo.com Fiji NIH, Fiji developers https://imagej.net/Fiji Prism (v.8.2.0) GraphPad https://www.graphpad.com/ scientific-software/prism/ Rstudio Rstudio https://rstudio.com/ R (v.4.3.3) R Core https://www.r-project.org/ Seurat (v.5.1.0) Hao et al. 81 https://satijalab.org/seurat/index.html ggplot2 (v.3.5.1) Wickham 82 https://ggplot2.tidyverse.org/index.html DESeq2 (v.1.36.0) Love et al. 83 https://github.com/mikelove/DESeq2 pheatmap (v.1.0.12) Kolde, R. 84 https://cran.r-project.org/web/ packages/pheatmap/index.html (Continued on next page) Cell Stem Cell 32, 1–13.e1–e7, July 3, 2025 e2

Transfection:

Article Title: Predicting prime editing efficiency across diverse edit types and chromatin contexts with machine learning
Article Snippet: .. Next, transfection was performed with 1 μl of Lipofectamine 2000 with: PE: 750 ng of editor (pCMV-PE2-tagRFP-BleoR) and 250 ng of pegRNA ABE8e, BE4max, Cas9: 500 ng of editor, 250 ng of sgRNA, 200 ng of pCMV-Tol2 (Addgene no. 31823) and 50 ng CMV-GFP. (ABE8e: p2T-CMV-ABE8e-SpCas9-BlastR, BE4max: p2T-CMV-BE4max-BlastR (Addgene no. 152991), Cas9: p2T-CMV-SpCas9-WT-BlastR) Guide sequences are listed in Supplementary Table 1 . ..

Single Cell:

Article Title: Optimized human intestinal organoid model reveals interleukin-22-dependency of paneth cell formation.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Chemicals Advanced DMEM/F-12 GIBCO Cat#12634028 Opti-MEM I Reduced Serum Medium GIBCO Cat#11058021 Penicillin-Streptomycin (10,000 U/mL) GIBCO Cat#15140122 HEPES (1M) GIBCO Cat#15630056 GlutaMAX Supplement GIBCO Cat#35050038 Primocin (50 mg/mL) InvivoGen Cat#ant-pm-1 B27 Supplement (50X) ThermoFisher Cat#12587010 TrypLE Express Enzyme (1X), phenol red ThermoFisher Cat#12605010 Wnt surrogate-fc fusion protein U-PROTEIN EXPRESS BV Cat#N001-0.5mg Noggin-fc fusion protein conditioned medium U-PROTEIN EXPRESS BV Cat#N002 - 100 mL Animal-Free Recombinant Human EGF Peprotech Cat#AF-100-15 A83-01 Tocris Cat#2939 SB202190 Sigma Cat# S7067 Prostaglandin E2 10mg Tocris Cat#2296 Gastrin I (human) 1 mg Tocris Cat#3006 CHIR 99021 10mg Stemgent Cat# 04-0004-base Recombinant Human IL-22 Peprotech Cat#200-22 Recombinant Murine IL-22 Peprotech Cat#210-22 N-Acetyl-L-cysteine Sigma Cat#A9165 Nicotinamide (1M) Sigma Cat#N0636 Recombinant RNasin RNase Inhibitor, 10,000 U Promega Cat#N2515 GoScript Reverse Transcriptase kit Promega Cat#A5000 Oligo(dT) 15 Primer, 500ug/ml 20ug Promega Cat#C1101 Random Primer, 500ug/ml 20ug Promega Cat#C1181 AgeI-HF NEB Cat#R3552L BbsI-HF NEB Cat#R3539S RNeasy Mini Kit (250) Qiagen Cat#74106 BP-1-102 Selleckchem Cat#S7769 LY294002 Cayman Chemical Cat#70920 MK-2206 2HCl Selleckchem Cat#S1078 Rapamycin (AY-22989) Selleckchem Cat#S1039 Y-27632 dihydrochloride Abmole Bioscience Cat#M1817 iQ SYBR Green Supermix BioRad Cat#1708887 DAPI, 40,6-Diamidine-20-phenylindole dihydrochloride Sigma Cat#10236276001 Click-iT Plus EdU Alexa Fluor 647 Flow Cytometry Assay Kit ThermoFisher Cat#C10634 Antibodies Chr-A Antibody (C-20) Santa Cruz Cat#sc-1488; RRID:AB_2276319 Lysozyme EC 3.2.1.17 Dako Cat#A0099; RRID:AB_578661 Phospho-Stat3 (Tyr705) (D3A7) XP Rabbit mAb Cell Signaling Technology Cat#9145T; RRID:AB_2491009 Stat3 (79D7) Rabbit mAb Cell Signaling Technology Cat#4904T; RRID:AB_331269 Phospho-Akt (Ser473) Antibody Cell Signaling Technology Cat#9271T; RRID:AB_329825 Akt (pan) (11E7) Rabbit mAb Cell Signaling Technology Cat#4685S; RRID:AB_2225340 Phospho-S6 Ribosomal Protein (Ser235/236) (D57.2.2E) XP Rabbit mAb Cell Signaling Technology Cat#4858S; RRID:AB_916156 (Continued on next page) e1 Cell Stem Cell 29, 1333–1345.e1–e6, September 1, 2022 .. REAGENT or RESOURCE SOURCE IDENTIFIER S6 Ribosomal Protein (5G10) Rabbit mAb Cell Signaling Technology Cat#2217S; RRID:AB_331355 b-Actin (13E5) Rabbit mAb (HRP Conjugate) Cell Signaling Technology Cat#5125S; RRID:AB_1903890 Swine Anti-Rabbit Immunoglobulins/HRP Dako Cat#P0217; RRID:AB_2728719 Deposited data Proteomics profiling of hSIOs with or without addition of IL-22 and drug treatments this paper ProteomeXchange: PXD028746 scRNA-seq profiling of hSIOs with or without addition of IL-22 this paper GEO:GSE189423 Code required to reproduce the single-cell RNA-sequencing analysis this paper https://github.com/jeffdemartino/ PanethAnalysis Recombinant DNA sgBbsI (p2Tol-U6-2xBbsI-sgRNA-HygR) Arbab et al., 2015 Addgene, Cat#71485 spCas9-BlastR (pCBhCas9-BlastR) Arbab et al., 2015 Addgene, Cat#71489 Software and algorithms FlowJo software 10.4 FlowJo https://www.flowjo.com ImageJ 1.53 ImageJ https://imagej.nih.gov/ij/index.html Prism 8.2.0 GraphPad https://www.graphpad.com/scientific- software/prism/ Rstudio Rstudio https://rstudio.com/ R (v4.1.0) R https://www.r-project.org/ Seurat (v4.0.3) Stuart et al., 2019 https://satijalab.org/seurat/ scVelo python (v0.2.2, python v3.7) Bergen et al., 2020 https://scvelo.readthedocs.io/ destiny (v3.1.1) Angerer et al., 2016 https://bioconductor.org/packages/release/ bioc/html/destiny.html Slingshot (v2.0.0) Street et al., 2018 https://bioconductor.org/packages/release/ bioc/html/slingshot.html Experimental models: Organoid lines Donor1: N39 Beumer et al., 2020 HUB Donor2: NKI1 this paper NKI-Hubrecht Donor3: NKI2 this paper NKI-Hubrecht Donor4: NKI3 this paper NKI-Hubrecht Oligonucleotides qPCR primers this paper Table S1 Oligo Collection ..

RNA sequencing:

Recombinant:

Software:

Real-time Polymerase Chain Reaction:

CRISPR:

Article Title: CHD1 deletion stabilizes HIF1α to promote angiogenesis and glycolysis in prostate cancer.
Article Snippet: A Lactate Colorimetric/Fluorometric Assay Kit was purchased from BioVision (Milpitas, CA, USA) and was used according to the manufacturer’s instructions. .. Clustered regularly interspaced palindromic repeat (CRISPR)/ CRISPR-associated protein 9 (Cas9)-based CHD1 knockout CRISPR/Cas9-based CHD1 knockout was conducted with a Cas9 lentiviral vector (#71489, Addgene, Watertown, MA, USA) and a guide RNA (gRNA) vector (#84752, Addgene). ..

Article Title: CHD1 deletion stabilizes HIF1α to promote angiogenesis and glycolysis in prostate cancer
Article Snippet: A Lactate Colorimetric/Fluorometric Assay Kit was purchased from BioVision (Milpitas, CA, USA) and was used according to the manufacturer’s instructions. .. CRISPR/Cas9-based CHD1 knockout was conducted with a Cas9 lentiviral vector (#71489, Addgene, Watertown, MA, USA) and a guide RNA (gRNA) vector (#84752, Addgene). ..

Knock-Out:

Plasmid Preparation:

Article Title: High resolution photocatalytic mapping of SARS-CoV-2 Spike protein-host cell membrane interactions
Article Snippet: .. The cas9-carrying lentiviral vector spCas9-BlastR (plasmid no. 71489) was obtained from Addgene. pCG1-SARS-CoV-2_S plasmid bearing the codon-optimized form of the SARS-CoV-2 spike gene, and pVSV-ΔG-GFP-Fluc plasmid, carrying the replication-competent vesicular stomatitis virus (VSV) genome with the viral glycoprotein (G) gene being replaced with GFP and firefly luciferase, were obtained from Stefan Pöhlmann ( Hoffmann et al. , 2020 ). ..

Construct:

Article Title: Nr6a1 controls Hox expression dynamics and is a master regulator of vertebrate trunk development
Article Snippet: Once the ESCs were ready, they were pelleted and suspended in buffer R (ThermoFisher, MPK1025) at a density of 1*10 7 cells/ml. .. 100 μl of the ESCs were then mixed with the scCRISPR construct (Composition: 10 μl elute of HDR PCR product; 1 μl sgPal7 (Addgene, 71484); 1 μl spCas9-BlastR (Addgene, 71489). ..

Review