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blasticidin  (InvivoGen)


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  • 99

    Structured Review

    InvivoGen blasticidin
    Blasticidin, supplied by InvivoGen, used in various techniques. Bioz Stars score: 99/100, based on 3895 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bl/Blasticidin/pmc13068831-8-0-2
    Average 99 stars, based on 3895 article reviews
    blasticidin - by Bioz Stars, 2026-11
    99/100 stars

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    Related Articles

    Selection:

    Article Title: Functional divergence of the Bhanja virus NSs protein modulates interferon antagonism and viral fitness across divergent lineages
    Article Snippet: GMEM (ThermoFisher) with 10% FBS and 10% tryptose phosphate broth (TPB) for BHK-21 and BSR-T7/5. .. Selection maintained with 1 mg/ml G-418 (Promega; T7-expressing lines), 10 μg/ml Blasticidin (InvivoGen; A549-NPro, Huh7 variants), or 10 μg/ml Blasticidin + 2 μg/ml Puromycin (A549-VNPro). ..

    Article Title: A high-confidence inventory of the proteins in the mitochondrion-related organelle of Giardia.
    Article Snippet: .. One day post transfection, the drug selection was initiated by replacing the spent media with fresh media containing the appropriate selective antibiotic for the construct: Puromycin (50 μg/mL, InvivoGen, Cat. #ant-pr-5b) or Blasticidin (70 μg/mL, InvivoGen, Cat. # ant-bl-5b). ..

    Article Title: Functional redundancy, complementation, and cross-talk between CH25h and IFITM3 in infection and in IFITM3 trafficking and secretion.
    Article Snippet: Genetic ablation of CH25h was obtained following three rounds of HIV-mediated gene transduction of A549 cells with Cas9 and CRISPR-bearing HIV-1-based lentiviral vectors (gift of Feng Zhang, obtained from Addgene catalog numbers: 52963 and 52962). .. Selection of pools of control-KO or CH25h-KO cells was obtained following blasticidin (Invivogen, catalog number: ant-bl-1) and puromycin (Sigma, catalog number: P8833) selection thanks to the resistance genes coded by the two vectors. ..

    Article Title: CHD8-Dependent Chromatin Licensing Sustains Trophoblast Stem Cell Transcriptional Programs.
    Article Snippet: .. The infected hTSCs were treated with 5 μg/mL Blasticidin solution (Invivogen, #ant-bl-1) for two days for positive selection. ..

    Transfection:

    Article Title: A high-confidence inventory of the proteins in the mitochondrion-related organelle of Giardia.
    Article Snippet: .. One day post transfection, the drug selection was initiated by replacing the spent media with fresh media containing the appropriate selective antibiotic for the construct: Puromycin (50 μg/mL, InvivoGen, Cat. #ant-pr-5b) or Blasticidin (70 μg/mL, InvivoGen, Cat. # ant-bl-5b). ..

    Article Title: Helicobacter pylori Outer Membrane Vesicles Engage TLR2 Through NapA to Promote Epithelial Association and Inflammatory Signalling
    Article Snippet: NF‐κB reporter activity was verified by stimulating cells with TNF (20 ng/mL, R&D Systems) for 5 h, followed by cell lysis and measurement of NanoLuc luciferase activity using the Nano‐Glo Luciferase Assay System (Promega) on a TECAN Infinite M200 PRO plate reader according to the manufacturer's instructions. .. A pool of NF‐κB reporter cells (derived from three independent clones) was then transfected with either pDuo‐TLR2/1, pDuo‐TLR2/6 or the control pDuo‐MCS plasmid (InvivoGen) and selected with blasticidin (10 μg/mL) for 6 days. ..

    Construct:

    Article Title: A high-confidence inventory of the proteins in the mitochondrion-related organelle of Giardia.
    Article Snippet: .. One day post transfection, the drug selection was initiated by replacing the spent media with fresh media containing the appropriate selective antibiotic for the construct: Puromycin (50 μg/mL, InvivoGen, Cat. #ant-pr-5b) or Blasticidin (70 μg/mL, InvivoGen, Cat. # ant-bl-5b). ..

    Derivative Assay:

    Article Title: Helicobacter pylori Outer Membrane Vesicles Engage TLR2 Through NapA to Promote Epithelial Association and Inflammatory Signalling
    Article Snippet: NF‐κB reporter activity was verified by stimulating cells with TNF (20 ng/mL, R&D Systems) for 5 h, followed by cell lysis and measurement of NanoLuc luciferase activity using the Nano‐Glo Luciferase Assay System (Promega) on a TECAN Infinite M200 PRO plate reader according to the manufacturer's instructions. .. A pool of NF‐κB reporter cells (derived from three independent clones) was then transfected with either pDuo‐TLR2/1, pDuo‐TLR2/6 or the control pDuo‐MCS plasmid (InvivoGen) and selected with blasticidin (10 μg/mL) for 6 days. ..

    Control:

    Article Title: Helicobacter pylori Outer Membrane Vesicles Engage TLR2 Through NapA to Promote Epithelial Association and Inflammatory Signalling
    Article Snippet: NF‐κB reporter activity was verified by stimulating cells with TNF (20 ng/mL, R&D Systems) for 5 h, followed by cell lysis and measurement of NanoLuc luciferase activity using the Nano‐Glo Luciferase Assay System (Promega) on a TECAN Infinite M200 PRO plate reader according to the manufacturer's instructions. .. A pool of NF‐κB reporter cells (derived from three independent clones) was then transfected with either pDuo‐TLR2/1, pDuo‐TLR2/6 or the control pDuo‐MCS plasmid (InvivoGen) and selected with blasticidin (10 μg/mL) for 6 days. ..

    Article Title: Functional redundancy, complementation, and cross-talk between CH25h and IFITM3 in infection and in IFITM3 trafficking and secretion.
    Article Snippet: Genetic ablation of CH25h was obtained following three rounds of HIV-mediated gene transduction of A549 cells with Cas9 and CRISPR-bearing HIV-1-based lentiviral vectors (gift of Feng Zhang, obtained from Addgene catalog numbers: 52963 and 52962). .. Selection of pools of control-KO or CH25h-KO cells was obtained following blasticidin (Invivogen, catalog number: ant-bl-1) and puromycin (Sigma, catalog number: P8833) selection thanks to the resistance genes coded by the two vectors. ..

    Plasmid Preparation:

    Article Title: Helicobacter pylori Outer Membrane Vesicles Engage TLR2 Through NapA to Promote Epithelial Association and Inflammatory Signalling
    Article Snippet: NF‐κB reporter activity was verified by stimulating cells with TNF (20 ng/mL, R&D Systems) for 5 h, followed by cell lysis and measurement of NanoLuc luciferase activity using the Nano‐Glo Luciferase Assay System (Promega) on a TECAN Infinite M200 PRO plate reader according to the manufacturer's instructions. .. A pool of NF‐κB reporter cells (derived from three independent clones) was then transfected with either pDuo‐TLR2/1, pDuo‐TLR2/6 or the control pDuo‐MCS plasmid (InvivoGen) and selected with blasticidin (10 μg/mL) for 6 days. ..

    Cell Culture:

    Article Title: Antibody-TLR3 agonist-conjugates program the tumor microenvironment and induce immune responses leading to tumor regression in experimental EGFRvIII-positive glioma.
    Article Snippet: Expression levels of EGFRvIII in Bs153resE cells were stabilized by treating with the EGFR inhibitor erlotinib (10 μM, Santa Cruz) before experiments. .. The human reporter cell line HEKBluehTLR3 (Invivogen, Germany) and derivatives cultured in DMEM with 4.5 g/l glucose, 10% [v/v] heat-inactivated FCS, 100 μg/ml normocin, 30 μg/ml blasticidin and 100 μg/ml zeocin (all antibiotics from Invivogen). .. Cells were cultured under standardized conditions at 37 ◦C, 80% rH, and 5% CO2 in an incubator.

    Infection:

    Article Title: CHD8-Dependent Chromatin Licensing Sustains Trophoblast Stem Cell Transcriptional Programs.
    Article Snippet: .. The infected hTSCs were treated with 5 μg/mL Blasticidin solution (Invivogen, #ant-bl-1) for two days for positive selection. ..

    other:

    Article Title: A USP28-BRD4 axis contributes to the acceleration of breast cancer growth by hyperglycemia.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Recombinant GST-USP28 This Paper Recombinant GST-USP28-K702R This Paper Recombinant His-KAT8 This Paper Recombinant GST-SIRT1 This Paper Recombinant GST-BD1 This Paper Recombinant GST-BD2 This Paper Recombinant His-BD1 This Paper Recombinant His-BD2 This Paper Recombinant GST-UBA52 This Paper Recombinant GST-PreScission This Paper Canagliflozin MCE CAT# HY-10451 Acetyl coenzyme A MCE CAT# HY-114293 Streptozotocin targetmol CAT# T1507 β-Nicotinamide adenine dinucleotide(NAD) Aladdin CAT# N1492516-1g Polybrene Sigma CAT# H9268 Protein G Agarose beads GenScript CAT# L00664 Protein A/G-Magnetic Beads MCE CAT# HY-K0202 Protein A-Agarose beads GenScript CAT# L00210 PES 0.45 μm filter Millipore CAT# SLHP033RB TRizol reagent Thermo CAT# 15596018 Restriction Enzymes NEB Doxycycline Hyclate MCE CAT# HY-N0565B Cycloheximide Sigma CAT# C7698 BAY-876 MCE CAT# HY-100017 2-deoxy-glucose MCE CAT# HY-13966 USP28-Peptide Abmart USP28-K702Ac peptide Abmart Ni-NTA Genscript CAT# L00666 Glutathione Resin Genscript CAT# L00206 Streptavidin-conjugated magnetic beads Genscript CAT# L00936 MG132 MCE CAT# HY-13259 Citric acid monohydrate Sinopharm CAT# 5949–29-1 PVDF Millipore CAT# IPVH00010 Nitrocellulose (NC) membrane Bio-Rad CAT# 1620112 Sodium citrate Aladdin Reagent Inc CAT# 68–04-2 Sodium Acetate SinoPharm CAT# 10018718 Glucose SinoPharm CAT# 63005518 Guanidinium-HCl aladdin CAT# 50–01-1 Imidazole MACKLIN CAT# 288–32-4 Urea Sinopharm CAT# 57–13-6 Puromycin InvivoGen CAT# ANT-PR-1



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