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Phalanx Biotech onearraytm microarray service
Co-expression of K5 and K8 in prostate epithelium. a FACS profile of CFP and RFP fluorescing prostate cells from double transgenic K5/CFP + K18/RFP mice. The CFP−/RFP+ cells in the upper left, and the CFP+/RFP+ cells are the cell populations whose gene expression profiles were compared by <t>microarray</t> analysis. b Single cell RT-PCR analysis of prostate cells from double transgenic K5/CFP + K18/RFP mice. K5 amplicon is 346 bp, and K18 amplicon is 427 bp. c, d Sections of: c proximal region of ventral prostate lobe from adult FVB/NJ male (bar = 20 μm), and d urogenital sinus from FVB/NJ day 16 fetus, immunostained for both K5 and K8 (bar = 100 μm). Cells co-expressing K5 and K8 are yellow, some of which are indicted by the arrows in panel c. See Fig. S8 for images of separate fluorescent channels of urogenital sinus
Onearraytm Microarray Service, supplied by Phalanx Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/biotech+microarray+service/onearraytm+microarray+service/pmc03090697-122-11-4
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onearraytm microarray service - by Bioz Stars, 2026-10
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Article Title: Epithelial cell-targeted transgene expression enables isolation of cyan fluorescent protein (CFP)-expressing prostate stem/progenitor cells

Journal:

doi: 10.1007/s11248-010-9478-2

Co-expression of K5 and K8 in prostate epithelium. a FACS profile of CFP and RFP fluorescing prostate cells from double transgenic K5/CFP + K18/RFP mice. The CFP−/RFP+ cells in the upper left, and the CFP+/RFP+ cells are the cell populations whose gene expression profiles were compared by microarray analysis. b Single cell RT-PCR analysis of prostate cells from double transgenic K5/CFP + K18/RFP mice. K5 amplicon is 346 bp, and K18 amplicon is 427 bp. c, d Sections of: c proximal region of ventral prostate lobe from adult FVB/NJ male (bar = 20 μm), and d urogenital sinus from FVB/NJ day 16 fetus, immunostained for both K5 and K8 (bar = 100 μm). Cells co-expressing K5 and K8 are yellow, some of which are indicted by the arrows in panel c. See Fig. S8 for images of separate fluorescent channels of urogenital sinus
Figure Legend Snippet: Co-expression of K5 and K8 in prostate epithelium. a FACS profile of CFP and RFP fluorescing prostate cells from double transgenic K5/CFP + K18/RFP mice. The CFP−/RFP+ cells in the upper left, and the CFP+/RFP+ cells are the cell populations whose gene expression profiles were compared by microarray analysis. b Single cell RT-PCR analysis of prostate cells from double transgenic K5/CFP + K18/RFP mice. K5 amplicon is 346 bp, and K18 amplicon is 427 bp. c, d Sections of: c proximal region of ventral prostate lobe from adult FVB/NJ male (bar = 20 μm), and d urogenital sinus from FVB/NJ day 16 fetus, immunostained for both K5 and K8 (bar = 100 μm). Cells co-expressing K5 and K8 are yellow, some of which are indicted by the arrows in panel c. See Fig. S8 for images of separate fluorescent channels of urogenital sinus

Techniques Used: Expressing, Transgenic Assay, Gene Expression, Microarray, Reverse Transcription Polymerase Chain Reaction, Amplification

Related Articles

Amplification:

Article Title: Epithelial cell-targeted transgene expression enables isolation of cyan fluorescent protein (CFP)-expressing prostate stem/progenitor cells
Article Snippet: .. Samples were sent to Phalanx Biotech Group (CA) for amplification and OneArrayTM Microarray service, including single color labeling, three replications, hybridizations, scanning, signal extractions, and basic data analysis. .. The mouse OneArray™ consists of 29,922 mouse genome probes and 1,880 experimental control probes (see http://ww.phalanxbiotech.com for more information.

Microarray:

Article Title: Epithelial cell-targeted transgene expression enables isolation of cyan fluorescent protein (CFP)-expressing prostate stem/progenitor cells
Article Snippet: .. Samples were sent to Phalanx Biotech Group (CA) for amplification and OneArrayTM Microarray service, including single color labeling, three replications, hybridizations, scanning, signal extractions, and basic data analysis. .. The mouse OneArray™ consists of 29,922 mouse genome probes and 1,880 experimental control probes (see http://ww.phalanxbiotech.com for more information.

Labeling:

Article Title: Epithelial cell-targeted transgene expression enables isolation of cyan fluorescent protein (CFP)-expressing prostate stem/progenitor cells
Article Snippet: .. Samples were sent to Phalanx Biotech Group (CA) for amplification and OneArrayTM Microarray service, including single color labeling, three replications, hybridizations, scanning, signal extractions, and basic data analysis. .. The mouse OneArray™ consists of 29,922 mouse genome probes and 1,880 experimental control probes (see http://ww.phalanxbiotech.com for more information.



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