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mrna microarray service  (Phalanx Biotech)

 
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    Structured Review

    Phalanx Biotech mrna microarray service
    Expression levels of ( A ) BDH2 and ( B ) LCN2 <t>mRNA</t> in BM in MDS and control patients, including de novo CN-AML and normal BM. The expression levels of the BDH2 and LCN2 genes were normalized to the internal control β-actin to obtain the relative threshold cycle (ΔC T ). BDH2, hydroxybutyrate dehydrogenase type 2; BM, bone marrow; CN-AML, cytogenetically normal acute myeloid leukemia; LCN2, lipocalin 2; MDS, myelodysplastic syndrome; RA, refractory anemia; RAEB, refractory anemia with excess blasts; RARS, refractory anemia with ringed sideroblasts.
    Mrna Microarray Service, supplied by Phalanx Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biotech+microarray+service/mrna+microarray+service/pmc07246807-174-12-15
    Average 90 stars, based on 1 article reviews
    mrna microarray service - by Bioz Stars, 2026-11
    90/100 stars

    Images

    1) Product Images from "The Effects of Human BDH2 on the Cell Cycle, Differentiation, and Apoptosis and Associations with Leukemia Transformation in Myelodysplastic Syndrome"

    Article Title: The Effects of Human BDH2 on the Cell Cycle, Differentiation, and Apoptosis and Associations with Leukemia Transformation in Myelodysplastic Syndrome

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms21093033

    Expression levels of ( A ) BDH2 and ( B ) LCN2 mRNA in BM in MDS and control patients, including de novo CN-AML and normal BM. The expression levels of the BDH2 and LCN2 genes were normalized to the internal control β-actin to obtain the relative threshold cycle (ΔC T ). BDH2, hydroxybutyrate dehydrogenase type 2; BM, bone marrow; CN-AML, cytogenetically normal acute myeloid leukemia; LCN2, lipocalin 2; MDS, myelodysplastic syndrome; RA, refractory anemia; RAEB, refractory anemia with excess blasts; RARS, refractory anemia with ringed sideroblasts.
    Figure Legend Snippet: Expression levels of ( A ) BDH2 and ( B ) LCN2 mRNA in BM in MDS and control patients, including de novo CN-AML and normal BM. The expression levels of the BDH2 and LCN2 genes were normalized to the internal control β-actin to obtain the relative threshold cycle (ΔC T ). BDH2, hydroxybutyrate dehydrogenase type 2; BM, bone marrow; CN-AML, cytogenetically normal acute myeloid leukemia; LCN2, lipocalin 2; MDS, myelodysplastic syndrome; RA, refractory anemia; RAEB, refractory anemia with excess blasts; RARS, refractory anemia with ringed sideroblasts.

    Techniques Used: Expressing, Control

    RNA microarray analysis of BDH2 targeting genes. Group 1: genes related to mitochondria; group 2: genes that have been reported as oncogenes or tumor suppressor genes; group 3: genes that function to control the survival, growth, differentiation, proliferation, and effector function of tissues and cells. All of those genes showed statistical significance with p < 0.05.
    Figure Legend Snippet: RNA microarray analysis of BDH2 targeting genes. Group 1: genes related to mitochondria; group 2: genes that have been reported as oncogenes or tumor suppressor genes; group 3: genes that function to control the survival, growth, differentiation, proliferation, and effector function of tissues and cells. All of those genes showed statistical significance with p < 0.05.

    Techniques Used: Microarray, Control

    Related Articles

    Microarray:

    Article Title: The Effects of Human BDH2 on the Cell Cycle, Differentiation, and Apoptosis and Associations with Leukemia Transformation in Myelodysplastic Syndrome
    Article Snippet: Slides measuring 5 μm in thickness were cut from representative tissue blocks, IHC staining was performed using a monoclonal antibody against human BDH2 (sc-104197, Santa Cruz Biotechnology, Dallas, TX, USA), and counterstaining was done with hematoxylin ( ). .. In order to find the target genes of BDH2, we used an mRNA microarray service (Phalanx Biotech Group, Inc., Taiwan) on shRNA-BDH2-3 THP1 cells and empty vector transfected THP1 cells. ..

    shRNA:

    Article Title: The Effects of Human BDH2 on the Cell Cycle, Differentiation, and Apoptosis and Associations with Leukemia Transformation in Myelodysplastic Syndrome
    Article Snippet: Slides measuring 5 μm in thickness were cut from representative tissue blocks, IHC staining was performed using a monoclonal antibody against human BDH2 (sc-104197, Santa Cruz Biotechnology, Dallas, TX, USA), and counterstaining was done with hematoxylin ( ). .. In order to find the target genes of BDH2, we used an mRNA microarray service (Phalanx Biotech Group, Inc., Taiwan) on shRNA-BDH2-3 THP1 cells and empty vector transfected THP1 cells. ..

    Plasmid Preparation:

    Article Title: The Effects of Human BDH2 on the Cell Cycle, Differentiation, and Apoptosis and Associations with Leukemia Transformation in Myelodysplastic Syndrome
    Article Snippet: Slides measuring 5 μm in thickness were cut from representative tissue blocks, IHC staining was performed using a monoclonal antibody against human BDH2 (sc-104197, Santa Cruz Biotechnology, Dallas, TX, USA), and counterstaining was done with hematoxylin ( ). .. In order to find the target genes of BDH2, we used an mRNA microarray service (Phalanx Biotech Group, Inc., Taiwan) on shRNA-BDH2-3 THP1 cells and empty vector transfected THP1 cells. ..

    Transfection:

    Article Title: The Effects of Human BDH2 on the Cell Cycle, Differentiation, and Apoptosis and Associations with Leukemia Transformation in Myelodysplastic Syndrome
    Article Snippet: Slides measuring 5 μm in thickness were cut from representative tissue blocks, IHC staining was performed using a monoclonal antibody against human BDH2 (sc-104197, Santa Cruz Biotechnology, Dallas, TX, USA), and counterstaining was done with hematoxylin ( ). .. In order to find the target genes of BDH2, we used an mRNA microarray service (Phalanx Biotech Group, Inc., Taiwan) on shRNA-BDH2-3 THP1 cells and empty vector transfected THP1 cells. ..



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