label-free surface plasma resonance (spr) based biosensors  (KU Leuven)

Journal: Biosensors

Article Title: DNA Origami and Their Application in Biosensors

doi: 10.3390/bios16050247

Figure Lengend Snippet: A schematic representation of an SPR DNA origami biosensor setup. A gold film has a light source directed at it through a glass prism. This generates the surface plasmons in the form of oscillating plasmon waves due to the free plasmons in the metal. The presence of DNA origami with the analytes that are immobilised onto the gold surface at precise and controlled positions shifts the angle of the reflected light, leading to the most optimal SPR signal to be monitored with a detector. Created with BioRender.com.

Article Snippet: 1990 , The first commercial SPR biosensor , This was created by a company known as Pharmacia Biacore, which determined molecules’ characteristics through affinity and kinetics [ ]. .

Techniques:

Journal: Npj Biosensing

Article Title: Plasmonic biosensors and actuators for integrated point-of-care diagnostics

doi: 10.1038/s44328-025-00050-1

Figure Lengend Snippet: a Biacore SPR sensor chip provides real-time, ready-to-go analysis of a wide range of molecular interactions, providing kinetics, affinity, and binding data (Credit: Cytiva Life Sciences (cytivalifesciences.com)). b Carterra SPR sensors integrate with HT-SPR technology, enabling fragments and small molecules screening and antibody discovery. c Detection principle of NG-Test CARBA 5 Lateral Flow Assay, NG Biotech. Reprinted with permission from ref. . Copyright 2018 Oxford Academic. d Principle of detection method for Nucleic Acid Lateral Flow Immunoassay (NALFIA), Pocket Diagnostic. Reprinted with permission from ref. . Copyright 2024 Springer Nature. e Klarite SERS substrate on an array of inverted pyramid structures (credit: optics.org). Reprinted with permission from ref. . Copyright 2011 Applied Spectroscopy. f Commercial Ocean Insight SERS substrate made of gold or silver, mounted in a microscope slide. Reprinted with permission from ref. . Copyright 2023 Springer Nature. g Nanopartz Plasmonic PCR and its 8-channels for POC system (credit: Nanopartz Inc. (nanopartz.com)). h Nexless P – IV transforms is an innovative Plasmonic PCR Technology, combining advanced plasmonic gold nanorod nanoparticles with real-time quantitative PCR. (Credit: Nexless Healthcare (nexlesshealthcare.ca)).

Article Snippet: Biacore SPR biosensors are designed with three core technologies: an optical detector system, a 50-nm layer of gold on the sensor chip, a microfluidic chip and liquid handling system .

Techniques: Binding Assay, Lateral Flow Assay, Diagnostic Assay, Spectroscopy, Microscopy, Real-time Polymerase Chain Reaction