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carboxymethylated dextran biosensor chips (cm5)  (Biacore)

 
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    Structured Review

    Biacore carboxymethylated dextran biosensor chips (cm5)
    Carboxymethylated Dextran Biosensor Chips (Cm5), supplied by Biacore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/biosensor+chip+cm5/biacore+3000+instrument/us12364736-917-2-6
    Average 90 stars, based on 1 article reviews
    carboxymethylated dextran biosensor chips (cm5) - by Bioz Stars, 2026-11
    90/100 stars

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    other:

    Article Title: Methods for treating heterotopic ossification
    Article Snippet: Such techniques include ELISAs, surface plasmon resonance binding assays (e.g., the BiacoreTM binding assay, Biacore AB, Uppsala, Sweden), sandwich assays (e.g., the paramagnetic bead system of IGEN International, Inc., Gaithersburg, Maryland), western blots, immunoprecipitation assays, and immunohistochemistry.

    Article Title: ALK7 binding proteins and uses thereof
    Article Snippet: In particular embodiments, the variant ALK7-binding protein has at least one characteristic selected from: (a) decreases the formation of a complex containing ALK7, a type II receptor (e.g., ActRIIA or ActRIIB), and one or more TGF-beta superfamily ligands (e.g., activin B, activin AB, Nodal, GDF1, GDF3 and/or GDF8) on the surface of cells expressing ALK7 and the ActRII receptor in the presence of the one or more TGF-beta superfamily ligands; (b) competes with one or more type II receptors for binding to ALK7; (c) competes with one or more TGF-beta superfamily ligands (e.g., activin B, activin AB, Nodal, GDF1, GDF3 and/or GDF8) for binding to ALK7; (d) decreases the phosphorylation of ALK7 in cells expressing ALK7 and a type II receptor (e.g., ActRIIA or ActRIIB) in the presence of one or more TGF-beta super family ligands (e.g., GDF1, GDF3, GDF8, activin B, activin AB, and/or Nodal); (e) decreases the phosphorylation of Smads (e.g., Smad2 and/or Smad3) in cells expressing ALK7 and a type II receptor (e.g., ActRIIA and/or ActRIIB) in the presence of one or more TGF-beta ligands (e.g., GDF1, GDF3, GDF8, activin B, activin AB, and/or Nodal); (f) binds to ALK7 with a KD of ≤1 nM and ≥1 pM (e.g., as determined by BIACORE® analysis), and (g) decreases the formation of a complex containing ALK7, a co-receptor (e.g., cripto and/or cryptic), and one or more TGF-beta superfamily ligands (e.g., Nodal).

    Article Title: ALK7 binding proteins and uses thereof
    Article Snippet: In some embodiments, an ALK7-binding protein binds to ALK7 with a KD of <1 nM and ≥1 pM (e.g., as determined by BIACORE® analysis).

    Binding Assay:

    Article Title: ALK7 binding proteins and uses thereof
    Article Snippet: The BIACORE® assay described above is an exemplary assay used to determine if two ALK7-binding proteins such as anti-ALK7 antibodies compete with/block each other for binding ALK7. .. On rare occasions, particular ALK7-binding proteins may not bind to ALK7-Fc coupled via anti-Fc IgG to a CM5 BIACORE® chip (this might occur when the relevant binding site on ALK7 is masked or destroyed by ALK7 linkage to Fc). ..

    Article Title: Anti-CD40 antibodies and uses thereof
    Article Snippet: 5.4 Quantitative Analysis of NK003 Dynamics and Affinity by Surface Plasmon Resonance Biacore T200 (GE Healthcare) was used to detect the affinity of the antibodies NK003, NK004. .. Data processing was performed using BIAevaluation software S200, the necessary software of Biacore T200 instrument and binding constants (Ka), dissociation constants (Kd), and equilibrium dissociation constants (KD) were calculated. .. The antigen to be tested, i.e. human CD40 recombinant protein (Acrobiosystems, CD 0-H5228) was diluted gradiently with a Running buffer (HBS-EP+, GE) at concentrations of 2 μM, 1 μM, 500 nM, 250 nM, 125 nM, 62.5 nM, 31.25 nM.

    Article Title: ALK7 binding proteins and uses thereof
    Article Snippet: .. Likewise, known assays are available for readily selecting anti-ALK7-antibodies displaying desirable features (e.g., assays for determining binding affinity to ALK7; cross-blocking assays such as the BIACORE®-based human ALK7-binding protein competition binding assays described herein). ..

    SPR Assay:

    Article Title: NKp46 binding agents
    Article Snippet: .. Competition and Affinity Study by Surface Plasmon Resonance (SPR) Biacore T100 General Procedure and Reagents SPR measurements were performed on a Biacore T100 apparatus (Biacore GE Healthcare) at 25° C. In all Biacore experiments HBS-EP+(Biacore GE Healthcare) and NaOH 10 mM NaCl 500 mM served as running buffer and regeneration buffer respectively. .. After two washes in staining buffer, cells incubated with anti-NKp46 antibodies were stained for 30 min at 4° C. with mouse anti-human IgG1-PE monoclonal antibodies from Miltenyi (dil. 1/10).

    Article Title: Antibodies binding to phospho-tau comprising phosphorylated Ser396 and Ser404 and methods of detecting thereof
    Article Snippet: In accordance with the present invention, the antibody-based molecule of the present invention binds to its Tau epitope with an affinity corresponding to a KD of about 10−7 M or less, such as about 10−8 M or less, such as about 10−9 M or less when determined by, for instance, surface plasmon resonance (SPR) technology in a Biacore 3000 instrument (preferably using the antibody as the ligand and the antigen as the analyte). .. In accordance with the present invention, the antibody-based molecule of the present invention binds to its Tau epitope with an affinity corresponding to a KD of about 10−7 M or less, such as about 10−8 M or less, such as about 10−9 M or less when determined by, for instance, surface plasmon resonance (SPR) technology in a Biacore 3000 instrument (preferably using the antibody as the ligand and the antigen as the analyte). ..

    Software:

    Article Title: Anti-CD40 antibodies and uses thereof
    Article Snippet: 5.4 Quantitative Analysis of NK003 Dynamics and Affinity by Surface Plasmon Resonance Biacore T200 (GE Healthcare) was used to detect the affinity of the antibodies NK003, NK004. .. Data processing was performed using BIAevaluation software S200, the necessary software of Biacore T200 instrument and binding constants (Ka), dissociation constants (Kd), and equilibrium dissociation constants (KD) were calculated. .. The antigen to be tested, i.e. human CD40 recombinant protein (Acrobiosystems, CD 0-H5228) was diluted gradiently with a Running buffer (HBS-EP+, GE) at concentrations of 2 μM, 1 μM, 500 nM, 250 nM, 125 nM, 62.5 nM, 31.25 nM.



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