dna binding buffer (Zymo Research)
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dna binding buffer
Dna Binding Buffer, supplied by Zymo Research, used in various techniques. Bioz Stars score: 96/100, based on 311 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/binding+buffer/DNA+Binding+Buffer/pm41818367-461-27-30
Average 96 stars, based on 311 article reviews
Dna Binding Buffer, supplied by Zymo Research, used in various techniques. Bioz Stars score: 96/100, based on 311 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/binding+buffer/DNA+Binding+Buffer/pm41818367-461-27-30
Average 96 stars, based on 311 article reviews
dna binding buffer - by Bioz Stars,
2026-11
96/100 stars
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Binding Assay:Article Title: First Report of High-Risk Rare blaSIM, blaSPM, and blaGIM Metallo-β-Lactamase Genes in Clinical Pseudomonas aeruginosa Isolates from Nigeria Article Snippet: After centrifugation at 10,000 × g for 1 minute, 400 μL of supernatant was transferred to Zymo-Spin IV filters and centrifuged. .. Filtrate was mixed with 1,200 μL of Article Title: Comparative analysis of stress markers, metabolic health, and gut microbiota in healthy and disabled dogs in long-term shelters in Thailand. Article Snippet: Lysates were centrifuged at ≥10,000 × g for 1 minute, and up to 400 μL of supernatant was transferred to a Zymo-SpinTM III-F filter, followed by centrifugation at 8,000 × g for 1 minute. .. The filtrate was mixed with 1,200 μL of Article Title: Prion propagation is controlled by a hierarchical network involving the nuclear Tfap2c and hnRNP K factors and the cytosolic mTORC1 complex Article Snippet: .. For the purification of the amplified product, the PCR reaction was mixed with five volumes of Article Title: Comparative analysis of stress markers, metabolic health, and gut microbiota in healthy and disabled dogs in long-term shelters in Thailand Article Snippet: Lysates were centrifuged at ≥10,000 × g for 1 minute, and up to 400 μL of supernatant was transferred to a Zymo-SpinTM III-F filter, followed by centrifugation at 8,000 × g for 1 minute. .. The filtrate was mixed with 1,200 μL of Article Title: Isolation, molecular profiling and antibiotics resistance of bacterial pathogens from street-vended soymilk Article Snippet: .. Supernatant (400 μL) was transferred to a Zymo-SpinTM IV Spin Filter, centrifuged at 7,000 × g for 1 minute, mixed with 1,200 μL Article Title: Evaluating Sequencing Strategies for Endometrial Microbiome Profiling in Endometrial Cancer: A Comparative Study of Short- and Long-Read 16S rRNA Approaches. Article Snippet: Genomic DNA was extracted using a ZymoBIOMICS DNA Microprep Kit (Zymo Research), with minor modifications to enhance microbial lysis. .. Briefly, 1 mL of swab/cell lysate (in DNA/RNA shield) was transferred to BashingBead Lysis Tubes and subjected to mechanical disruption using a Disruptor Genie bead beater (Scientific Industries) at maximum speed for 20 min. Lysates were centrifuged at 10 000 × g for 1 min, and 400 μL of supernatant was mixed with Article Title: Evaluating Sequencing Strategies for Endometrial Microbiome Profiling in Endometrial Cancer: A Comparative Study of Short‐ and Long‐Read 16S rRNA Approaches Article Snippet: Genomic DNA was extracted using a ZymoBIOMICS DNA Microprep Kit (Zymo Research), with minor modifications to enhance microbial lysis. .. Briefly, 1 mL of swab/cell lysate (in DNA/RNA shield) was transferred to BashingBead Lysis Tubes and subjected to mechanical disruption using a Disruptor Genie bead beater (Scientific Industries) at maximum speed for 20 min. Lysates were centrifuged at 10 000 × g for 1 min, and 400 μL of supernatant was mixed with Article Title: Prion propagation is controlled by a hierarchical network involving the nuclear Tfap2c and hnRNP K factors and the cytosolic mTORC1 complex. Article Snippet: .. For the purification of the amplified product, the PCR reaction was mixed with five volumes of Centrifugation:Article Title: Comparative analysis of stress markers, metabolic health, and gut microbiota in healthy and disabled dogs in long-term shelters in Thailand. Article Snippet: Lysates were centrifuged at ≥10,000 × g for 1 minute, and up to 400 μL of supernatant was transferred to a Zymo-SpinTM III-F filter, followed by centrifugation at 8,000 × g for 1 minute. .. The filtrate was mixed with 1,200 μL of Article Title: Comparative analysis of stress markers, metabolic health, and gut microbiota in healthy and disabled dogs in long-term shelters in Thailand Article Snippet: Lysates were centrifuged at ≥10,000 × g for 1 minute, and up to 400 μL of supernatant was transferred to a Zymo-SpinTM III-F filter, followed by centrifugation at 8,000 × g for 1 minute. .. The filtrate was mixed with 1,200 μL of Purification:Article Title: Prion propagation is controlled by a hierarchical network involving the nuclear Tfap2c and hnRNP K factors and the cytosolic mTORC1 complex Article Snippet: .. For the purification of the amplified product, the PCR reaction was mixed with five volumes of Article Title: Prion propagation is controlled by a hierarchical network involving the nuclear Tfap2c and hnRNP K factors and the cytosolic mTORC1 complex. Article Snippet: .. For the purification of the amplified product, the PCR reaction was mixed with five volumes of Amplification:Article Title: Prion propagation is controlled by a hierarchical network involving the nuclear Tfap2c and hnRNP K factors and the cytosolic mTORC1 complex Article Snippet: .. For the purification of the amplified product, the PCR reaction was mixed with five volumes of Article Title: Prion propagation is controlled by a hierarchical network involving the nuclear Tfap2c and hnRNP K factors and the cytosolic mTORC1 complex. Article Snippet: .. For the purification of the amplified product, the PCR reaction was mixed with five volumes of Polymerase Chain Reaction:Article Title: Prion propagation is controlled by a hierarchical network involving the nuclear Tfap2c and hnRNP K factors and the cytosolic mTORC1 complex Article Snippet: .. For the purification of the amplified product, the PCR reaction was mixed with five volumes of Article Title: Prion propagation is controlled by a hierarchical network involving the nuclear Tfap2c and hnRNP K factors and the cytosolic mTORC1 complex. Article Snippet: .. For the purification of the amplified product, the PCR reaction was mixed with five volumes of Lysis:Article Title: Evaluating Sequencing Strategies for Endometrial Microbiome Profiling in Endometrial Cancer: A Comparative Study of Short- and Long-Read 16S rRNA Approaches. Article Snippet: Genomic DNA was extracted using a ZymoBIOMICS DNA Microprep Kit (Zymo Research), with minor modifications to enhance microbial lysis. .. Briefly, 1 mL of swab/cell lysate (in DNA/RNA shield) was transferred to BashingBead Lysis Tubes and subjected to mechanical disruption using a Disruptor Genie bead beater (Scientific Industries) at maximum speed for 20 min. Lysates were centrifuged at 10 000 × g for 1 min, and 400 μL of supernatant was mixed with Article Title: Evaluating Sequencing Strategies for Endometrial Microbiome Profiling in Endometrial Cancer: A Comparative Study of Short‐ and Long‐Read 16S rRNA Approaches Article Snippet: Genomic DNA was extracted using a ZymoBIOMICS DNA Microprep Kit (Zymo Research), with minor modifications to enhance microbial lysis. .. Briefly, 1 mL of swab/cell lysate (in DNA/RNA shield) was transferred to BashingBead Lysis Tubes and subjected to mechanical disruption using a Disruptor Genie bead beater (Scientific Industries) at maximum speed for 20 min. Lysates were centrifuged at 10 000 × g for 1 min, and 400 μL of supernatant was mixed with Disruption:Article Title: Evaluating Sequencing Strategies for Endometrial Microbiome Profiling in Endometrial Cancer: A Comparative Study of Short- and Long-Read 16S rRNA Approaches. Article Snippet: Genomic DNA was extracted using a ZymoBIOMICS DNA Microprep Kit (Zymo Research), with minor modifications to enhance microbial lysis. .. Briefly, 1 mL of swab/cell lysate (in DNA/RNA shield) was transferred to BashingBead Lysis Tubes and subjected to mechanical disruption using a Disruptor Genie bead beater (Scientific Industries) at maximum speed for 20 min. Lysates were centrifuged at 10 000 × g for 1 min, and 400 μL of supernatant was mixed with Article Title: Evaluating Sequencing Strategies for Endometrial Microbiome Profiling in Endometrial Cancer: A Comparative Study of Short‐ and Long‐Read 16S rRNA Approaches Article Snippet: Genomic DNA was extracted using a ZymoBIOMICS DNA Microprep Kit (Zymo Research), with minor modifications to enhance microbial lysis. .. Briefly, 1 mL of swab/cell lysate (in DNA/RNA shield) was transferred to BashingBead Lysis Tubes and subjected to mechanical disruption using a Disruptor Genie bead beater (Scientific Industries) at maximum speed for 20 min. Lysates were centrifuged at 10 000 × g for 1 min, and 400 μL of supernatant was mixed with |
![( A ) Coculture (1:1) of parental B16F10 cells expressing pBabe-Cerulean (blue) and B16F10 cells expressing GsdmD-C3 and mCherry (red) (see movie S1). ( B ) Times of individual cell shrinkage and cell membrane lysis, as in (A). Unpaired t test. ( C ) Apoptotic phase (shrinkage-lysis interval) from (B). One-way ANOVA. ( D ) Quantification of annexin V + SIINFEKL peptide–pulsed B16F10 [as in (B) and (C)] that remains fluorescent when coincubated with activated OT-I T cells at 4 hours. Two-way ANOVA. ( E ) Quantification of SIINFEKL peptide–pulsed B16F10 cell populations with ectopic caspase-3-activatable GSDMD (GD-C3) by fluorescence signal at 24 hours of coincubation with activated OT-I T cells. ( F ) As in (E) with Gsdme. [(E) and (F)] Paired t test. ( G ) Number of GCamp6s spikes observed from SIINFEKL peptide–pulsed Neuro2a cells, coincubated with activated OT-I T cells, 10:1 T cell–to–target cell ratio. Images were taken every 2 min for a duration of 3 hours immediately following the additional of OT-I T cells, and numbers of observable GCamp6s spikes were counted and plotted (see movies S2 to S4 and fig. S5C). Unpaired t test. ( H ) Design for (I) and (J). Created in BioRender; B. Liu (2026), https://BioRender.com/j67ipmw . ( I ) Bioluminescence signal intensity of Neuro2a in C57BL/6 animals ( n = 9 animals per group). Two-way ANOVA. ( J ) Kaplan-Meyer survival curves from C57BL/6 mice injected with Neuro2a cells ( n = 9 animals per group). ( K ) Design for (L). Created in BioRender; B. Liu (2026), https://BioRender.com/j67ipmw . ( L ) Bioluminescence signal intensity of Neuro2a in C57BL/6 animals (control, n = 9 animals; Gsdme −/− , n = 8 animals). ( M ) Kaplan-Meyer survival curves from C57BL/6 mice rechallenged with Neuro2a cells [ n = 5 animals per group; log-rank (Mantel-Cox) test]. ( N ) As in (M) with B16F10 [ n = 12 animals in naïve group, 14 animals in rechallenge group; log-rank (Mantel-Cox) test]. * P < 0.05, ** P < 0.01, and **** P < 0.0001. iv, intravenous; ip, intraperitoneal.](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_6084/pmc13626084/pmc13626084__sciadv.aeb1137-f3.jpg)
![PGCs are more sensitive to DNA double-strand breaks than somatic cells. (A) Cell viability curves after 24 h treatment with increasing concentrations of etoposide (ETP; x-axis shows log 10 [ETP]). PGC viability dropped sharply even at low ETP doses, whereas CEF cells were more tolerant (viability data are mean ± SD of triplicates). (B) Apoptosis detection in PGCs treated with low-dose ETP. The proportion of Annexin V + /PI + cells (late apoptosis) was significantly elevated even at 0.03 µM ETP. (C) Western blot analysis of γ-H 2 AX protein in PGCs treated with varying ETP concentrations (left panel; β-actin as loading control). The bar graph (right) shows the ratio of γ-H 2 AX to β-actin band intensity, with a marked increase at 3 µM ETP (p-values are indicated in the figure). (D) Quantification of γ-H 2 AX foci per cell in THP-1 cells, male PGCs, and female PGCs after exposure to X-ray doses of 0, 2, 4, 6, and 8 Gy. After 48 h recovery, γ-H 2 AX foci increased significantly with higher radiation in THP-1, male PGCs, and female PGCs. Female PGC data points are red squares; male PGCs are blue squares; THP-1 are black circles. (E) Cell-cycle distribution of male vs. female PGCs after DNA damage. PGCs were irradiated (2, 4, 6 Gy), cultured 48 h, and analyzed by flow cytometry for cell-cycle phase (propidium iodide staining). Stacked bars show the percentage of cells in G 0 G 1 , S, and G 2 /M phases in untreated vs. irradiated cells. After damage, female PGCs predominantly arrested in G 2 /M (increased G 2 fraction), whereas male PGCs accumulated in S phase. Statistical significance by one-way ANOVA (p-values are indicated in the figure).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_1053/pmc13011053/pmc13011053__gr3.jpg)