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bfa  (Applichem inc)

 
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    Structured Review

    Applichem inc bfa
    Bfa, supplied by Applichem inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/bfa/bfa/pmc06071996-79-0-4
    Average 90 stars, based on 1 article reviews
    bfa - by Bioz Stars, 2026-10
    90/100 stars

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    other:

    Article Title: The Carboxy Terminus of the Ligand Peptide Determines the Stability of the MHC Class I Molecule H-2K b : A Combined Molecular Dynamics and Experimental Study
    Article Snippet: RMA-S cells [ ] were kept overnight at 25°C and at t = 0 treated with 10 μg/ml BFA (Applichem or Alfa Aesar) and with 10 μM peptide and transferred to 37°C for a maximum of 4 hours.

    Article Title: Distinct mechanisms survey the structural integrity of HLA-B*27:05 intracellularly and at the surface
    Article Snippet: BFA was purchased from AppliChem (Darmstadt, Germany) and used at 10 μg/ml.

    Article Title: Evidence for a COP-I-independent transport route from the Golgi complex to the endoplasmic reticulum.
    Article Snippet: *Cell Biology and Biophysics Programme, EMBL Heidelberg, Meyerhofstrasse 1, 69117 Heidelberg, Germany ‡Department of Biochemistry, Virginia Polytechnic Institute and State University, Blacksburg, Virginia 24061, USA ¶Institut Curie, UMR 144 Curie/CNRS, 26, rue d’Ulm, F-75248 Paris Cedex 05, France §Department of Biological Sciences, University of Warwick, Coventry CV4 7AL, UK #e-mail: Pepperko@EMBL-Heidelberg.de **email: Nilsson@EMBL-Heidelberg.de †These authors contributed equally to this work

    Concentration Assay:

    Article Title: F-Actin Organization and Pollen Tube Tip Growth in Arabidopsis Are Dependent on the Gametophyte-Specific Armadillo Repeat Protein ARO1
    Article Snippet: For drug treatments, 2.5 mM LatB (Sigma-Aldrich) was dissolved in 100% DMSO and diluted to a final concentration of 20 nM in liquid PGM. .. BFA (AppliChem) was dissolved in 5 mg/mL methanol and diluted to a final concentration of 5 μg/mL in liquid PGM. ..



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    KNOLLE as part of SNARE complex (E>>G:SNAP33/ big3 , estradiol-induced, +EST) either trapped at TGN (+ brefeldin A, BFA) or trafficked to the cell division plane (-BFA) was tested for ubiquitination. Lines for comparison: Con, non-transgenic control; G:KN, GFP-tagged KNOLLE.Protein extracts from seedlings treated with wortmannin (Wm) and NEM (+Wm/NEM) or untreated (-Wm/NEM) were immunoprecipitated with the GFP trap (IP(α-GFP)) and the enriched fractions probed with anti-GFP, anti-ubiquitin and anti-KNOLLE antibodies; bottom panel, prolonged exposure. Expected sizes: GFP:KNOLLE, 65 kDa; (UBQ)n-GFP:KNOLLE, 80-120 kDa (UBQ moiety 15-60 kDa); endogenous KNOLLE, 33 kDa; (UBQ)n-KNOLLE, 50-100 kDa. Brackets, ubiquitinated bands of GFP:KNOLLE; wide braces, range of (UBQ)n of KNOLLE between 50 and 100 kDa; narrow braces, the highest (UBQ)n band of KNOLLE at approximately 100 kDa. Asterisk, GFP:KNOLLE; double asterisks, GFP:SNAP33; hashtags, cross-reacting bands of anti-KNOLLE antiserum; numbers on the left, protein sizes in kDa.

    Journal: bioRxiv

    Article Title: Degradation of cytokinesis-specific Qa-SNARE KNOLLE is regulated by context-dependent ubiquitination

    doi: 10.64898/2026.05.13.724867

    Figure Lengend Snippet: KNOLLE as part of SNARE complex (E>>G:SNAP33/ big3 , estradiol-induced, +EST) either trapped at TGN (+ brefeldin A, BFA) or trafficked to the cell division plane (-BFA) was tested for ubiquitination. Lines for comparison: Con, non-transgenic control; G:KN, GFP-tagged KNOLLE.Protein extracts from seedlings treated with wortmannin (Wm) and NEM (+Wm/NEM) or untreated (-Wm/NEM) were immunoprecipitated with the GFP trap (IP(α-GFP)) and the enriched fractions probed with anti-GFP, anti-ubiquitin and anti-KNOLLE antibodies; bottom panel, prolonged exposure. Expected sizes: GFP:KNOLLE, 65 kDa; (UBQ)n-GFP:KNOLLE, 80-120 kDa (UBQ moiety 15-60 kDa); endogenous KNOLLE, 33 kDa; (UBQ)n-KNOLLE, 50-100 kDa. Brackets, ubiquitinated bands of GFP:KNOLLE; wide braces, range of (UBQ)n of KNOLLE between 50 and 100 kDa; narrow braces, the highest (UBQ)n band of KNOLLE at approximately 100 kDa. Asterisk, GFP:KNOLLE; double asterisks, GFP:SNAP33; hashtags, cross-reacting bands of anti-KNOLLE antiserum; numbers on the left, protein sizes in kDa.

    Article Snippet: Estradiol induction and brefeldin A (BFA) treatment: 50 μM BFA (50 mM stock in DMSO/Ethanol, Invitrogen) was applied for 30 minutes, followed by 20 μM estradiol (20 mM stock in DMSO, Sigma-aldrich) for 3 hours and 30 minutes in the presence of BFA and subsequently wortmannin and NEM were added as described above.

    Techniques: Ubiquitin Proteomics, Comparison, Transgenic Assay, Control, Immunoprecipitation