tgf-beta 1-plex b111206  (QuantoBio)

Journal: JHEP Reports

Article Title: TAF15 in tumor-associated macrophages enhances protumorigenic polarization and promotes cholangiocarcinoma progression

doi: 10.1016/j.jhepr.2025.101545

Figure Lengend Snippet: TAF15 facilitates M2-like polarization of macrophages, enhancing TGFBI secretion and promoting the progression of ICC. (A) Reactome enrichment analysis of DEGs is depicted, with the top 10 enriched terms displayed. (B) Expression heatmaps of extracellular matrix organization. (C) Upper: a UMAP plot is colored by different clusters. Lower: a dot plot represents the mean expression of TGFBI across various clusters. (D) Western blot analysis examines the expression of TGFBI in different groups. (H) The colony formation ability of ICC cells treated with 0, 5, and 10 μg/ml recombinant TGFBI protein is assessed. (F–I) ELISA analysis measures the levels of TGFBI in the different CM of TAMs. Data are presented as mean ± SD, one-way ANOVA was performed for statistical analysis, n = 3. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001. CM, conditional media; DEGs, differentially expressed genes; ICC, intrahepatic cholangiocarcinoma; TAF15, TATA-binding protein-associated factor 15; TGFBI, transforming growth factor-beta induced.

Article Snippet: The transforming growth factor-beta induced (TGFBI) ELISA test kit was purchased from CUSABIO (CSB-EL023450MO, CSB-E16665h, Wuhan, China).

Techniques: Expressing, Western Blot, Recombinant, Enzyme-linked Immunosorbent Assay, Binding Assay

Journal: JHEP Reports

Article Title: TAF15 in tumor-associated macrophages enhances protumorigenic polarization and promotes cholangiocarcinoma progression

doi: 10.1016/j.jhepr.2025.101545

Figure Lengend Snippet: M2pepLNP-siTaf15 shows promise in targeting macrophages for the treatment of ICC. (A,B) In vivo and in vitro small animal imaging was conducted using free IR780 and M2pepLNP/IR780 in ICC mice via the tail vein. (C) Self-luminescence (Dil) with F4/80 multicolor immunofluorescence. (D,E) Western blot and QPCR analysis of Taf15 in BMDM after transfection with small interference for 48 h. (F) A schematic diagram illustrates the encapsulation of siTaf15 by M2pep-liposomes. (G) A schematic diagram depicts the treatment regimen of ICC mice with liposome materials. (H) Differential morphology of the liver is observed after treatment with M2pepLNP coated with siNc and siTaf15. (I) Weight of the liver is measured following treatment with M2LNP coated with siNc and siTaf15. (J) H&E and IHC analyses are conducted for CK19 and TAF15 in liver tissues treated with M2pepLNP-siNc and M2pepLNP-siTaf15. (K) H&E analysis of heart, lung, spleen, kidney in mice treated with M2pepLNP-siNc and M2pepLNP-siTaf15. (L) Flow cytometry analysis the expression of CD206 and CD86 of macrophage in mice liver. (M) ELISA analysis measures the levels of TGFBI in eyeball blood and liver tissue from mice. Data are presented as mean ± SD, unpaired two-tailed Student’s t test. ∗ p <0.05, ∗∗ p <0.01, ∗∗∗ p <0.001, ∗∗∗∗ p <0.0001. BMDM, bone marrow-derived macrophages; ICC, intrahepatic cholangiocarcinoma; IHC, immunohistochemistry; QPCR, quantitative PCR; TAF15, TATA-binding protein-associated factor 15; TGFBI, transforming growth factor-beta induced.

Article Snippet: The transforming growth factor-beta induced (TGFBI) ELISA test kit was purchased from CUSABIO (CSB-EL023450MO, CSB-E16665h, Wuhan, China).

Techniques: In Vivo, In Vitro, Imaging, Immunofluorescence, Western Blot, Transfection, Encapsulation, Liposomes, Flow Cytometry, Expressing, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Derivative Assay, Immunohistochemistry, Real-time Polymerase Chain Reaction, Binding Assay