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image cytometer  (Revvity)


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    Structured Review

    Revvity image cytometer
    Image Cytometer, supplied by Revvity, used in various techniques. Bioz Stars score: 96/100, based on 4276 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/benchtop+liquid+handler/Cellometer+Spectrum+Image+Cytometer/pm31704838-66-16-21
    Average 96 stars, based on 4276 article reviews
    image cytometer - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: Single‐Cell Profiling Reveals RAB13 + Endothelial Cells and Profibrotic Mesenchymal Cells in Aged Human Bone Marrow
    Article Snippet: .. After digestion, the collagenized bone fraction was filtered through a 70 μm filter into a collection tube, pooled with BM fraction, and centrifuged at 1500 rpm and 4°C for 5 min. To determine live cell concentration and viability, 10 μL of each sample was stained with acridine orange and propidium iodide (AO/PI) solution (Nexcelom) and analyzed with Cellometer K2 Image Cytometer (Nexcelom Bioscience). ..

    Staining:

    Article Title: Single‐Cell Profiling Reveals RAB13 + Endothelial Cells and Profibrotic Mesenchymal Cells in Aged Human Bone Marrow
    Article Snippet: .. After digestion, the collagenized bone fraction was filtered through a 70 μm filter into a collection tube, pooled with BM fraction, and centrifuged at 1500 rpm and 4°C for 5 min. To determine live cell concentration and viability, 10 μL of each sample was stained with acridine orange and propidium iodide (AO/PI) solution (Nexcelom) and analyzed with Cellometer K2 Image Cytometer (Nexcelom Bioscience). ..

    Article Title: Multiplexed high-content imaging uncovers morphological diversity of lymphocyte activation and dysfunction
    Article Snippet: .. Cell counts were obtained on a CellometerTM Spectrum image cytometer (Revvity) or the Cellaca TM MX high-throughput cell counter (Revvity) with AO/PI staining (Nexcelom CS2-0106). .. The Jurkat, Clone E6-1 cell line was purchased from ATCC (#TIB-152 TM ) and cultured in RPMI-1640 (Gibco TM , #11875093), supplemented with 10% heat-inactivated fetal bovine serum (FBS, Sigma-Aldrich ® #F9665-500ML), 2 mM L-glutamine (Gibco TM , #25030081), 1mM sodium pyruvate (Gibco TM , #11360070), 1X MEM non-essential amino acids (Gibco TM , #11140050), 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco TM , #15140122).

    Cytometry:

    Article Title: Single‐Cell Profiling Reveals RAB13 + Endothelial Cells and Profibrotic Mesenchymal Cells in Aged Human Bone Marrow
    Article Snippet: .. After digestion, the collagenized bone fraction was filtered through a 70 μm filter into a collection tube, pooled with BM fraction, and centrifuged at 1500 rpm and 4°C for 5 min. To determine live cell concentration and viability, 10 μL of each sample was stained with acridine orange and propidium iodide (AO/PI) solution (Nexcelom) and analyzed with Cellometer K2 Image Cytometer (Nexcelom Bioscience). ..

    Article Title: Multiplexed high-content imaging uncovers morphological diversity of lymphocyte activation and dysfunction
    Article Snippet: .. Cell counts were obtained on a CellometerTM Spectrum image cytometer (Revvity) or the Cellaca TM MX high-throughput cell counter (Revvity) with AO/PI staining (Nexcelom CS2-0106). .. The Jurkat, Clone E6-1 cell line was purchased from ATCC (#TIB-152 TM ) and cultured in RPMI-1640 (Gibco TM , #11875093), supplemented with 10% heat-inactivated fetal bovine serum (FBS, Sigma-Aldrich ® #F9665-500ML), 2 mM L-glutamine (Gibco TM , #25030081), 1mM sodium pyruvate (Gibco TM , #11360070), 1X MEM non-essential amino acids (Gibco TM , #11140050), 100 U/ml penicillin and 100 μg/ml streptomycin (Gibco TM , #15140122).

    Article Title: Contributing to a (cell) cultural shift: Human platelet lysate is a suitable replacement for fetal calf serum when culturing the HTR8/SVneo extravillous trophoblast cell line.
    Article Snippet: While suitable as a universal supplement in cell culture, fetal calf serum (FCS) has several downsides, including its bovine origin and batch-to-batch variability.. In this study, we investigated human platelet lysate (HPL) as a potential replacement for FCS when culturing an extravillous trophoblast cell line.. HTR8/SVneo cells were cultured in conventional medium supplemented with 10% FCS or serum-free medium supplemented with varying concentrations of HPL for 24 h or 3 weeks, whereafter LDH release, metabolic activity and cytokine release were assessed.

    Article Title: Processes for production of tumor infiltrating lymphocytes and uses of same in immunotherapy
    Article Snippet: .. 5.3.2.1 Cell counts and viability: TIL samples were counted and viability determined using a Cellometer K2 automated cell counter (Nexcelom Bioscience, Lawrence, MA) according to SOP LAB-003 Rev 000 Cellometer K2 Image Cytometer Automatic Cell Counter. ..

    Fourier Transform Infrared Spectroscopy:

    Article Title: Design of tertiary Z-scheme CuO–ZnO@MXene photocatalyst with superior visible-light activity for decomposition of nanoplastics
    Article Snippet: Nitrogen adsorption-desorption isotherms were obtained using a Micromeritics TriStar II analyzer after degassing samples at 85 ◦C for 24 h. The Brunauer-Emmett-Teller (BET) and Barrett-Joyner-Halenda (BJH) methods were applied to determine surface area and pore-size distribution, respectively. .. Fourier-transform infrared (FTIR) spectra (4000–400 cm− 1) were recorded on a PerkinElmer Spectrum 100 spectrometer (ATR mode) to identify functional groups, including metal–oxygen bonds, surface hydroxyls, and MXene terminations. .. Diffuse reflectance UV–Vis spectra (200–800 nm) were collected using a Thermo Scientific spectrophotometer with an integrating sphere.

    Article Title: Application of highly dispersed copper catalysts in CO2 hydrogenation through surfactant introduction
    Article Snippet: To determine the reduction properties of catalysts, hydrogen temperature-programmed reduction (H2-TPR) analysis was conducted on 100 mg of the catalyst in 10 % H2/Ar from 50 to 500 ◦C at a heating rate of 10 ◦C/min using the Autochem II 2920 instrument (Micromeritics, Waste to Energy and Catalyst Laboratory of Ajou University). .. Fourier-transform infrared spectroscopy (FT-IR) was performed using a Spectrum 3 (PerkinElmer). .. X-ray photoelectron spectroscopy (XPS) analysis was conducted using the a K-Alpha system (Thermo Fisher Scientific).

    Functional Assay:

    Article Title: Design of tertiary Z-scheme CuO–ZnO@MXene photocatalyst with superior visible-light activity for decomposition of nanoplastics
    Article Snippet: Nitrogen adsorption-desorption isotherms were obtained using a Micromeritics TriStar II analyzer after degassing samples at 85 ◦C for 24 h. The Brunauer-Emmett-Teller (BET) and Barrett-Joyner-Halenda (BJH) methods were applied to determine surface area and pore-size distribution, respectively. .. Fourier-transform infrared (FTIR) spectra (4000–400 cm− 1) were recorded on a PerkinElmer Spectrum 100 spectrometer (ATR mode) to identify functional groups, including metal–oxygen bonds, surface hydroxyls, and MXene terminations. .. Diffuse reflectance UV–Vis spectra (200–800 nm) were collected using a Thermo Scientific spectrophotometer with an integrating sphere.

    other:

    Article Title: Cationically Modified PVA-Based Electrospun Nanofiber Membrane for Adsorptive PFAS Removal from Water
    Article Snippet: Functional group analysis of the adsorbent samples before and after PFAS adsorption was carried out using Fourier transform infrared spectroscopy (FTIR; PerkinElmer Spectrum 100, Waltham, MA, USA).

    Automated:

    Article Title: Contributing to a (cell) cultural shift: Human platelet lysate is a suitable replacement for fetal calf serum when culturing the HTR8/SVneo extravillous trophoblast cell line.
    Article Snippet: While suitable as a universal supplement in cell culture, fetal calf serum (FCS) has several downsides, including its bovine origin and batch-to-batch variability.. In this study, we investigated human platelet lysate (HPL) as a potential replacement for FCS when culturing an extravillous trophoblast cell line.. HTR8/SVneo cells were cultured in conventional medium supplemented with 10% FCS or serum-free medium supplemented with varying concentrations of HPL for 24 h or 3 weeks, whereafter LDH release, metabolic activity and cytokine release were assessed.



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